RLG00000017227

Flavonoid 3'-monooxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021147
Physical Location & Seq
Forward (+)
14335957 .. 14336370
414 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017227

Sequence Viewer

Length: 414 bp
ATGGAGCTGGTTTCCACTTGGCCCGTTCTAATTTTGGTCTGTCTAGCAACTCTAACCCTTCTCTCCAAACTTTACTTTTCCCAAAGCCGAAAACTGAAATCTCCACCAGGTCCTAGACCATGGCCTATTATCGGCAACCTAAACCTCATCGGTCCAATCCCTCACCAAAGCCTTCACAAACTCTCCCAAACTTATGGACCAATAATGCAGCTCAAGTTCGGATCCCGCCCGGTTGTCATTGCATCCTCTGCAGAAATGGCAAAGCAATTTCTCAAAACACATGATCATGTCTTTGCCTCTAGACCCCAAACTGCAGCTGGCAAGTACCTCACTTACAACTACCTCGACGTCACTTGGGCGCCTTACGGTGACTATTGGTGCCAAGGCCGCAAGATATTCAGACCTCGGTTGTGA

Protein Analysis

138

Amino Acids

15.57

Weight (kDa)

10.01

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 135 7.2e-17 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021147)

Species Orthologous Gene IDs
malus_domestica MD15G1218300.v1.1
rosa_chinensis RchiOBHm_Chr2g0145871
rosa_laevigata RLG00000017227
rosa_samantha Rh2BG169500 Rh2DG469600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 358, 378
AciI CCGC 2 cut(s) 226, 388
AclWI GGATC 2 cut(s) 216, 229
AcyI GRCGYC 2 cut(s) 348, 359
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 131
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 3 cut(s) 7, 211, 317
AluI AGCT 3 cut(s) 7, 211, 317
AlwI GGATC 2 cut(s) 216, 229
AoxI GGCC 3 cut(s) 20, 122, 385
ApeKI GCWGC 2 cut(s) 208, 314
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 4 cut(s) 21, 110, 152, 197
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 155, 380
AvaII GGWCC 3 cut(s) 110, 152, 197
BamHI GGATCC 1 cut(s) 221
BanI GGYRCC 2 cut(s) 358, 378
BbvI GCAGC 2 cut(s) 220, 326
BciT130I CCWGG 1 cut(s) 108
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 230
BfaI CTAG 3 cut(s) 44, 114, 300
BfmI CTRYAG 2 cut(s) 249, 312
BfoI RGCGCY 1 cut(s) 362
BisI GCNGC 3 cut(s) 209, 315, 388
BlsI GCNGC 3 cut(s) 210, 316, 389
Bme1390I CCNGG 2 cut(s) 108, 230
Bme18I GGWCC 3 cut(s) 110, 152, 197
BmgT120I GGNCC 4 cut(s) 21, 110, 152, 197
BmiI GGNNCC 3 cut(s) 223, 360, 380
BmrFI CCNGG 2 cut(s) 108, 230
BmsI GCATC 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 230
BsaHI GRCGYC 2 cut(s) 348, 359
BsaJI CCNNGG 3 cut(s) 119, 382, 404
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 3 cut(s) 119, 382, 404
BseGI GGATG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 220, 326
BshFI GGCC 3 cut(s) 22, 124, 387
BshNI GGYRCC 2 cut(s) 358, 378
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 131
BsnI GGCC 3 cut(s) 22, 124, 387
Bsp143I GATC 2 cut(s) 221, 283
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 2 cut(s) 226, 388
BspANI GGCC 3 cut(s) 22, 124, 387
BspLI GGNNCC 3 cut(s) 223, 360, 380
BspMAI CTGCAG 2 cut(s) 253, 316
BspPI GGATC 2 cut(s) 216, 229
BspT107I GGYRCC 2 cut(s) 358, 378
BsrDI GCAATG 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 119, 382, 404
BssMI GATC 2 cut(s) 221, 283
BssNI GRCGYC 2 cut(s) 348, 359
BssT1I CCWWGG 2 cut(s) 119, 382
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 368
BstACI GRCGYC 2 cut(s) 348, 359
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 319
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 242
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 2 cut(s) 224, 286
BstMBI GATC 2 cut(s) 221, 283
BstMWI GCNNNNNNNGC 3 cut(s) 248, 257, 387
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 106, 228
BstSFI CTRYAG 2 cut(s) 249, 312
BstV1I GCAGC 2 cut(s) 220, 326
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 221
BsuRI GGCC 3 cut(s) 22, 124, 387
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 319
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 4 cut(s) 21, 110, 152, 197
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 1 cut(s) 325
CviAII CATG 3 cut(s) 120, 281, 287
CviJI RGCY 8 cut(s) 7, 22, 87, 124, 171, 211, 317, 387
CviKI_1 RGCY 8 cut(s) 7, 22, 87, 124, 171, 211, 317, 387
CviQI GTAC 1 cut(s) 325
DinI GGCGCC 1 cut(s) 360
DpnI GATC 2 cut(s) 223, 285
DpnII GATC 2 cut(s) 221, 283
Eco130I CCWWGG 2 cut(s) 119, 382
Eco47I GGWCC 3 cut(s) 110, 152, 197
EcoO109I RGGNCCY 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 119, 382
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 119, 382
FaeI CATG 3 cut(s) 123, 284, 290
FaiI YATR 4 cut(s) 121, 195, 282, 288
FatI CATG 3 cut(s) 119, 280, 286
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 209, 315, 388
FokI GGATG 1 cut(s) 229
Fsp4HI GCNGC 3 cut(s) 209, 315, 388
FspBI CTAG 3 cut(s) 44, 114, 300
GlaI GCGC 1 cut(s) 360
GluI GCNGC 3 cut(s) 209, 315, 388
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 3 cut(s) 22, 124, 387
HapII CCGG 1 cut(s) 230
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 2 cut(s) 348, 359
Hin1II CATG 3 cut(s) 123, 284, 290
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HpaII CCGG 1 cut(s) 230
HphI GGTGA 2 cut(s) 155, 380
Hpy188I TCNGA 2 cut(s) 221, 401
Hpy188III TCNNGA 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 68, 182
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 208, 242, 251, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 257, 387
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 2 cut(s) 348, 359
Hsp92II CATG 3 cut(s) 123, 284, 290
HspAI GCGC 1 cut(s) 359
KasI GGCGCC 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 2 cut(s) 221, 283
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 4 cut(s) 93, 120, 243, 303
Lsp1109I GCAGC 2 cut(s) 220, 326
LweI GCATC 1 cut(s) 251
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 3 cut(s) 44, 114, 300
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 349, 368
MalI GATC 2 cut(s) 223, 285
MboI GATC 2 cut(s) 221, 283
MflI RGATCY 1 cut(s) 221
MluCI AATT 2 cut(s) 30, 266
Mly113I GGCGCC 1 cut(s) 359
MnlI CCTC 7 cut(s) 155, 171, 256, 307, 338, 353, 414
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 108, 230
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 3 cut(s) 248, 257, 387
NarI GGCGCC 1 cut(s) 359
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 119
NdeII GATC 2 cut(s) 221, 283
NlaIII CATG 3 cut(s) 123, 284, 290
NlaIV GGNNCC 3 cut(s) 223, 360, 380
NmuCI GTSAC 2 cut(s) 349, 368
PkrI GCNGC 3 cut(s) 210, 316, 389
PluTI GGCGCC 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 3 cut(s) 223, 360, 380
PspPI GGNCC 4 cut(s) 21, 110, 152, 197
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 2 cut(s) 253, 316
PsuI RGATCY 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 285
SatI GCNGC 3 cut(s) 209, 315, 388
Sau3AI GATC 2 cut(s) 221, 283
Sau96I GGNCC 4 cut(s) 21, 110, 152, 197
ScrFI CCNGG 2 cut(s) 108, 230
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 2 cut(s) 249, 312
SfoI GGCGCC 1 cut(s) 360
SinI GGWCC 3 cut(s) 110, 152, 197
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 2 cut(s) 30, 266
SsiI CCGC 2 cut(s) 226, 388
SspDI GGCGCC 1 cut(s) 358
SspMI CTAG 3 cut(s) 44, 114, 300
StyD4I CCNGG 2 cut(s) 106, 228
StyI CCWWGG 2 cut(s) 119, 382
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 345
TaqII GACCGA 1 cut(s) 140
TasI AATT 2 cut(s) 30, 266
TauI GCSGC 1 cut(s) 390
TseFI GTSAC 2 cut(s) 349, 368
TseI GCWGC 2 cut(s) 208, 314
Tsp45I GTSAC 2 cut(s) 349, 368
VpaK11BI GGWCC 3 cut(s) 110, 152, 197
XbaI TCTAGA 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XspI CTAG 3 cut(s) 44, 114, 300
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.