RchiOBHm_Chr2g0150911

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006025
Physical Location & Seq
Forward (+)
68567988 .. 68572077
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52016

Sequence Viewer

Length: 816 bp
ATGAAGATAAAGTGTAGAAATGCATTCAGGGATGAGCTTGCTTTACTTCAGAAGATACGACATCCCAATGTTGTCCAATTTTTGGGTGCTGTAACACAAAGCAGTCCAATGATGATTGTCACGGAATATCTACCCAAGGGAGATTTTCGTTTATATCTTAAAAGAAAAGGTGCCTTAAAGCCAACAGCAGCAGTGAAGTTTGCACTTGACATTGCCAGGGGGATGAATTATCTGCATGAGCATAAACCTGAAGCAATAATTCATCGAGATCTTGAGCCCTCAAATATATTGCGGGATGATTCTGGGCATCTGAAAGTTGCAGACTTTGGGGTGAGCAAGCTACTGATGGTCGCAAAGACAGTTAAAGAAGACAGACCTGTGACTACTGAAGGCACTTCTTGGAGATACGTGGCTCCTGAAGTTTACAGAAATGAAGAATATGATACCAAAGTGGATGTTTTTTCATTTGCCTTGATCTTGCAAGAGATGATTGAAGGTTGTCTACCATTTTCTACAAAACCAGAAAAAGAAGTACCTAAAGCATATGCTGCAAATGAGCGCCCACCTTTTAGAGCACCACCAAAGCTTTATGCACATGGATTAAAGGAGTTGATTGAGGAATGTTGGAGTCAGGACCCATTTAGGAGACCAACATTCAAGCAAATAATTAAGAGTTTGGATAACATTAATGACCAGTATGCTCGAAAAAGACACTGGAAGGTTATGCCACTTATGTGTCTCCAGAACCTGGAGAACATGGTGAAGAAGGGTCGCTCTAGTCCCAGCAGCCATTCATCCTGCTCTACGATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Pkinase_Tyr

Protein Analysis

271

Amino Acids

31.22

Weight (kDa)

9.44

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 6 - 226 9.3e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 7 - 224 5e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccB7I CCANNNNNTGG 2 cut(s) 82, 748
AccI GTMKAC 1 cut(s) 502
AciI CCGC 1 cut(s) 292
AcuI CTGAAG 4 cut(s) 32, 270, 408, 438
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 82, 748
AgsI TTSAA 2 cut(s) 494, 658
AjnI CCWGG 2 cut(s) 215, 747
AleI CACNNNNGTG 1 cut(s) 733
AluBI AGCT 3 cut(s) 37, 340, 586
AluI AGCT 3 cut(s) 37, 340, 586
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 2 cut(s) 640, 743
AlwNI CAGNNNCTG 1 cut(s) 748
ApeKI GCWGC 3 cut(s) 188, 548, 786
AseI ATTAAT 1 cut(s) 687
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 2 cut(s) 343, 772
AvaII GGWCC 1 cut(s) 634
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 279
BarI GAAGNNNNNNTAC 2 cut(s) 486, 518
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 200, 535, 798
BccI CCATC 1 cut(s) 340
BciT130I CCWGG 2 cut(s) 217, 749
BcoDI GTCTC 2 cut(s) 640, 743
BfaI CTAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 562
BglII AGATCT 1 cut(s) 268
BisI GCNGC 3 cut(s) 189, 549, 787
BlsI GCNGC 3 cut(s) 190, 550, 788
Bme1390I CCNGG 2 cut(s) 217, 749
Bme18I GGWCC 1 cut(s) 634
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 3 cut(s) 172, 414, 636
BmrFI CCNGG 2 cut(s) 217, 749
BmsI GCATC 1 cut(s) 316
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 725, 770
BpuEI CTTGAG 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 409
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 2 cut(s) 135, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 748
Bse1I ACTGG 2 cut(s) 694, 719
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 2 cut(s) 217, 749
BseDI CCNNGG 2 cut(s) 135, 216
BseGI GGATG 6 cut(s) 37, 61, 228, 301, 460, 794
BseLI CCNNNNNNNGG 2 cut(s) 82, 748
BseMI GCAATG 1 cut(s) 210
BseNI ACTGG 2 cut(s) 694, 719
BseXI GCAGC 3 cut(s) 200, 535, 798
BseYI CCCAGC 1 cut(s) 782
BshNI GGYRCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 577
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 2 cut(s) 82, 748
BsmAI GTCTC 2 cut(s) 640, 743
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 640
Bsp1286I GDGCHC 2 cut(s) 279, 577
Bsp143I GATC 2 cut(s) 268, 474
BspACI CCGC 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 172, 414, 636
BspT107I GGYRCC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 2 cut(s) 694, 719
BssECI CCNNGG 2 cut(s) 135, 216
BssMI GATC 2 cut(s) 268, 474
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 217, 749
Bst4CI ACNGT 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 39, 338
BstF5I GGATG 6 cut(s) 37, 61, 228, 301, 460, 794
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 2 cut(s) 271, 477
BstMAI GTCTC 2 cut(s) 640, 743
BstMBI GATC 2 cut(s) 268, 474
BstMWI GCNNNNNNNGC 1 cut(s) 548
BstNI CCWGG 2 cut(s) 217, 749
BstSCI CCNGG 2 cut(s) 215, 747
BstV1I GCAGC 3 cut(s) 200, 535, 798
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BtsCI GGATG 6 cut(s) 37, 61, 228, 301, 460, 794
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 712
Cac8I GCNNGC 2 cut(s) 39, 338
CaiI CAGNNNCTG 1 cut(s) 748
CfoI GCGC 1 cut(s) 561
Cfr13I GGNCC 1 cut(s) 634
Csp6I GTAC 1 cut(s) 533
CviAII CATG 3 cut(s) 236, 596, 757
CviJI RGCY 7 cut(s) 37, 181, 277, 340, 413, 586, 789
CviKI_1 RGCY 7 cut(s) 37, 181, 277, 340, 413, 586, 789
CviQI GTAC 1 cut(s) 533
DpnI GATC 2 cut(s) 270, 476
DpnII GATC 2 cut(s) 268, 474
Eco130I CCWWGG 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 634
Eco57I CTGAAG 4 cut(s) 32, 270, 408, 438
EcoO109I RGGNCCY 1 cut(s) 634
EcoRII CCWGG 2 cut(s) 215, 747
EcoT14I CCWWGG 1 cut(s) 135
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 3 cut(s) 239, 599, 760
FaqI GGGAC 1 cut(s) 765
FatI CATG 3 cut(s) 235, 595, 756
FauI CCCGC 1 cut(s) 285
FauNDI CATATG 1 cut(s) 544
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 3 cut(s) 189, 549, 787
FokI GGATG 6 cut(s) 44, 48, 235, 308, 467, 781
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 3 cut(s) 189, 549, 787
FspBI CTAG 1 cut(s) 777
GlaI GCGC 1 cut(s) 560
GluI GCNGC 3 cut(s) 189, 549, 787
GsaI CCCAGC 1 cut(s) 786
GsuI CTGGAG 2 cut(s) 725, 770
HaeII RGCGCY 1 cut(s) 562
HhaI GCGC 1 cut(s) 561
Hin1II CATG 3 cut(s) 239, 599, 760
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 2 cut(s) 299, 628
HphI GGTGA 2 cut(s) 343, 772
Hpy166II GTNNAC 2 cut(s) 424, 503
Hpy188I TCNGA 2 cut(s) 51, 312
Hpy188III TCNNGA 5 cut(s) 266, 272, 416, 632, 742
Hpy8I GTNNAC 2 cut(s) 424, 503
HpyAV CCTTC 4 cut(s) 383, 488, 712, 760
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 7 cut(s) 23, 203, 235, 320, 481, 551, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 548
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 3 cut(s) 239, 599, 760
HspAI GCGC 1 cut(s) 559
Kzo9I GATC 2 cut(s) 268, 474
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 3 cut(s) 200, 535, 798
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 777
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 91, 118, 379
MalI GATC 2 cut(s) 270, 476
MboI GATC 2 cut(s) 268, 474
MboII GAAGA 5 cut(s) 16, 64, 380, 446, 775
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 279, 577
MluCI AATT 4 cut(s) 77, 226, 258, 666
MlyI GAGTC 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 605
MnlI CCTC 2 cut(s) 289, 610
Mph1103I ATGCAT 1 cut(s) 25
MseI TTAA 6 cut(s) 159, 176, 363, 602, 669, 687
MslI CAYNNNNRTG 2 cut(s) 66, 733
MspR9I CCNGG 2 cut(s) 217, 749
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 2 cut(s) 217, 749
MwoI GCNNNNNNNGC 1 cut(s) 548
NdeI CATATG 1 cut(s) 544
NdeII GATC 2 cut(s) 268, 474
NlaIII CATG 3 cut(s) 239, 599, 760
NlaIV GGNNCC 3 cut(s) 172, 414, 636
NmuCI GTSAC 2 cut(s) 118, 379
NsiI ATGCAT 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 733
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 2 cut(s) 82, 748
PkrI GCNGC 3 cut(s) 190, 550, 788
PleI GAGTC 1 cut(s) 636
PpsI GAGTC 1 cut(s) 636
Ppu21I YACGTR 1 cut(s) 409
PpuMI RGGWCCY 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 687
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 2 cut(s) 215, 747
PspFI CCCAGC 1 cut(s) 782
PspGI CCWGG 2 cut(s) 215, 747
PspN4I GGNNCC 3 cut(s) 172, 414, 636
PspPI GGNCC 1 cut(s) 634
PspPPI RGGWCCY 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
RseI CAYNNNNRTG 2 cut(s) 66, 733
SaqAI TTAA 6 cut(s) 159, 176, 363, 602, 669, 687
SatI GCNGC 3 cut(s) 189, 549, 787
Sau3AI GATC 2 cut(s) 268, 474
Sau96I GGNCC 1 cut(s) 634
SchI GAGTC 1 cut(s) 637
ScrFI CCNGG 2 cut(s) 217, 749
SduI GDGCHC 2 cut(s) 279, 577
SfaNI GCATC 1 cut(s) 316
SinI GGWCC 1 cut(s) 634
SmiMI CAYNNNNRTG 2 cut(s) 66, 733
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 77, 226, 258, 666
SsiI CCGC 1 cut(s) 292
SspMI CTAG 1 cut(s) 777
StyD4I CCNGG 2 cut(s) 215, 747
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 411
TaqI TCGA 2 cut(s) 265, 703
TasI AATT 4 cut(s) 77, 226, 258, 666
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 6 cut(s) 159, 176, 363, 602, 669, 687
Tru9I TTAA 6 cut(s) 159, 176, 363, 602, 669, 687
TscAI CASTG 2 cut(s) 198, 719
TseFI GTSAC 2 cut(s) 118, 379
TseI GCWGC 3 cut(s) 188, 548, 786
Tsp45I GTSAC 2 cut(s) 118, 379
TspDTI ATGAA 6 cut(s) 17, 239, 251, 447, 453, 783
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 2 cut(s) 198, 719
Van91I CCANNNNNTGG 2 cut(s) 82, 748
VpaK11BI GGWCC 1 cut(s) 634
VspI ATTAAT 1 cut(s) 687
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 1 cut(s) 777
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.