Rw2G039620

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006025
Physical Location & Seq
Forward (+)
65065320 .. 65069732
4413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039620.1

Sequence Viewer

Length: 1374 bp
ATGGACCGCCAAGACTCCGTCACGGCCCGCTTCACTCTCGGCAGGCAGTCCTCGCTCGCCCCCGACCCGGACGACGGCTCGGTGGTCGAGCCGGCCGACGGGGCCCTTGACCCGAGGGTCCGACTCATGTACTTGGCCAACGAGGGCGACTTGGAAGGCATTCAGGAGCTCCTGGACTCCGGAACCAATGTCAATTTCACTGACATAGATGGACGCACCGCTCTCCACGTCGCCGCCTGTCAGGGCCGGACCGACGTCGTTCAGTTGCTCCTCCAAAGAGGCGCCGGCGTCAACCTCCAGGACCGCTGGGGCAGTACGCCCCTTGCGGATGCGGTGTACTATAAGAATGAGGATGTGATCAAGCTTTTAGAGCAGCACGGAGCTAAACTTCCGATGGCTCCTATGCATGTGGAGAATGCACGTGAAGTCCCCGAGTATGAGATTGATCCCAAGGAGCTTGATTTTTCAGACAGCGTTGACATAACCAAGGGAACCTACTGCATTGCATCTTGGCGTGGAATTCAAGTTGCAGTTAAGACGCTTGAGGAGAAAGTATTTGCTGATGAAGATAAAGTAAATGCATTCAGGGATGAGCTTGCTTTACTTCAGAAGATACGACATCCCAATGTTGTCCAATTTTTGGGTGCTGTAACACAAAGCAGTCCAATGATGATTGTCACGGAATATCTACCCAAGGGAGATTTTCGTTTATATCTTAAAAGAAAAGGTGCCTTAAAGCCAACAGCAGCAGTGAAGTTTGCACTTGACATTGCCAGGGGGATGAATTATCTGCATGAGCATAAACCTGAAGCAATAATTCATCGAGATCTTGAGCCCTCAAATATATTGCGGGATGATTCTGGGCATCTGAAAGTTGCAGACTTTGGGGTGAGCAAGCTACTGATAGTCGCAAAGACAGTTAAAGAAGACAGACCTGTGACTACTGAAGGCACTTCTTGGAGATATGTGGCTCCTGAAGTTTACAGAAATGAAGAATATGATACCAAAGTGGATGTTTTTTCATTTGCCTTGATCTTGCAAGAGATGATTGAAGGTTGTCTACCATTTTCTACAAAACCAGAAAAAGAAGTACCTAAAGCATATGCTGCAAATGAGCGCCCACCTTTTAGAGCACCACCAAAGCTTTATGCACATGGATTAAAGGAGTTGATTGAGGAATGTTGGAGTCAGGACCCATTTAGGAGACCAACATTCAAGCAAATAATTAAGAGTTTGGATAACATTAATGACCAGTATGCTCGAAAAAGACACTGGAAGGTTATGCCACTTATGTGTCTCCAGAACCTGGAGAACATGGTGAAGAAGGGTCGCTCTAGTCCCAGCAGCCATTCATCCTGCTCTACGATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Pkinase_Tyr

Protein Analysis

457

Amino Acids

51.57

Weight (kDa)

5.95

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 42 - 129 4.2e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 42 - 91 1.8e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 63 - 108 6.4e-08 Ankyrin repeats (many copies)
Ank PF00023 70 - 102 2.3e-09 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 161 - 412 1.7e-54 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 167 - 410 2.8e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 2 cut(s) 281, 728
AccB7I CCANNNNNTGG 2 cut(s) 640, 1306
AccBSI CCGCTC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 1060
AccIII TCCGGA 1 cut(s) 179
AciI CCGC 8 cut(s) 7, 28, 219, 234, 304, 326, 332, 850
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 2 cut(s) 93, 135
AcsI RAATTY 1 cut(s) 519
AcuI CTGAAG 4 cut(s) 590, 828, 966, 996
AcvI CACGTG 1 cut(s) 422
AcyI GRCGYC 3 cut(s) 255, 282, 288
AfaI GTAC 4 cut(s) 131, 316, 338, 1092
AfiI CCNNNNNNNGG 5 cut(s) 67, 74, 98, 640, 1306
AgsI TTSAA 3 cut(s) 524, 1052, 1216
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 4 cut(s) 171, 297, 773, 1305
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AleI CACNNNNGTG 1 cut(s) 1291
AluBI AGCT 7 cut(s) 169, 364, 383, 457, 595, 898, 1144
AluI AGCT 7 cut(s) 169, 364, 383, 457, 595, 898, 1144
Alw21I GWGCWC 2 cut(s) 171, 1135
Alw26I GTCTC 2 cut(s) 1198, 1301
AlwI GGATC 1 cut(s) 440
AlwNI CAGNNNCTG 1 cut(s) 1306
Ama87I CYCGRG 2 cut(s) 112, 431
Aor13HI TCCGGA 1 cut(s) 179
AoxI GGCC 5 cut(s) 24, 93, 102, 135, 244
ApaI GGGCCC 1 cut(s) 106
ApeKI GCWGC 4 cut(s) 373, 746, 1106, 1344
ApoI RAATTY 1 cut(s) 519
AseI ATTAAT 1 cut(s) 1245
Asp700I GAANNNNTTC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 284, 1119
AspS9I GGNCC 9 cut(s) 4, 25, 102, 103, 118, 244, 249, 301, 1192
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 901, 1330
AvaI CYCGRG 2 cut(s) 112, 431
AvaII GGWCC 5 cut(s) 4, 118, 249, 301, 1192
BaeGI GKGCMC 1 cut(s) 106
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BalI TGGCCA 1 cut(s) 137
BanI GGYRCC 2 cut(s) 281, 728
BanII GRGCYC 3 cut(s) 106, 171, 837
BarI GAAGNNNNNNTAC 2 cut(s) 1044, 1076
BbrPI CACGTG 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 2 cut(s) 171, 1135
BbvI GCAGC 4 cut(s) 385, 758, 1093, 1356
BccI CCATC 2 cut(s) 203, 388
BceAI ACGGC 2 cut(s) 39, 91
BciT130I CCWGG 4 cut(s) 173, 299, 775, 1307
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 1198, 1301
BfaI CTAG 1 cut(s) 1335
BfoI RGCGCY 2 cut(s) 285, 1120
BglI GCCNNNNNGGC 1 cut(s) 101
BglII AGATCT 1 cut(s) 826
BisI GCNGC 5 cut(s) 234, 374, 747, 1107, 1345
BlsI GCNGC 5 cut(s) 235, 375, 748, 1108, 1346
Bme1390I CCNGG 5 cut(s) 68, 173, 299, 775, 1307
Bme18I GGWCC 5 cut(s) 4, 118, 249, 301, 1192
BmeT110I CYCGRG 2 cut(s) 112, 431
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 9 cut(s) 4, 25, 102, 103, 118, 244, 249, 301, 1192
BmrFI CCNGG 5 cut(s) 68, 173, 299, 775, 1307
BmsI GCATC 3 cut(s) 319, 515, 874
BoxI GACNNNNGTC 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 933
BpmI CTGGAG 3 cut(s) 281, 1283, 1328
BpuEI CTTGAG 2 cut(s) 563, 851
BpuMI CCSGG 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 422
BsaHI GRCGYC 3 cut(s) 255, 282, 288
BsaI GGTCTC 1 cut(s) 1198
BsaJI CCNNGG 5 cut(s) 113, 450, 486, 693, 774
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 74, 98, 640, 1306
Bse118I RCCGGY 2 cut(s) 91, 284
Bse1I ACTGG 2 cut(s) 1252, 1277
Bse3DI GCAATG 2 cut(s) 501, 768
BseAI TCCGGA 1 cut(s) 179
BseBI CCWGG 4 cut(s) 173, 299, 775, 1307
BseDI CCNNGG 5 cut(s) 113, 450, 486, 693, 774
BseGI GGATG 8 cut(s) 334, 358, 595, 619, 786, 859, 1018, 1352
BseLI CCNNNNNNNGG 5 cut(s) 67, 74, 98, 640, 1306
BseMI GCAATG 2 cut(s) 501, 768
BseNI ACTGG 2 cut(s) 1252, 1277
BseRI GAGGAG 2 cut(s) 260, 560
BseSI GKGCMC 1 cut(s) 106
BseX3I CGGCCG 1 cut(s) 93
BseXI GCAGC 4 cut(s) 385, 758, 1093, 1356
BseYI CCCAGC 2 cut(s) 306, 1340
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 5 cut(s) 26, 95, 104, 137, 246
BshNI GGYRCC 2 cut(s) 281, 728
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 2 cut(s) 171, 1135
BsiHKCI CYCGRG 2 cut(s) 112, 431
BsiSI CCGG 5 cut(s) 68, 92, 180, 247, 285
BslFI GGGAC 2 cut(s) 413, 1323
BslI CCNNNNNNNGG 5 cut(s) 67, 74, 98, 640, 1306
BsmAI GTCTC 2 cut(s) 1198, 1301
BsmFI GGGAC 2 cut(s) 413, 1323
BsmI GAATGC 3 cut(s) 159, 421, 581
BsnI GGCC 5 cut(s) 26, 95, 104, 137, 246
Bso31I GGTCTC 1 cut(s) 1198
BsoBI CYCGRG 2 cut(s) 112, 431
Bsp120I GGGCCC 1 cut(s) 102
Bsp1286I GDGCHC 4 cut(s) 106, 171, 837, 1135
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 4 cut(s) 357, 445, 826, 1032
BspACI CCGC 8 cut(s) 7, 28, 219, 234, 304, 326, 332, 850
BspANI GGCC 5 cut(s) 26, 95, 104, 137, 246
BspEI TCCGGA 1 cut(s) 179
BspPI GGATC 1 cut(s) 440
BspT107I GGYRCC 2 cut(s) 281, 728
BspTNI GGTCTC 1 cut(s) 1198
BsrBI CCGCTC 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 501, 768
BsrFI RCCGGY 2 cut(s) 91, 284
BsrI ACTGG 2 cut(s) 1252, 1277
BssAI RCCGGY 2 cut(s) 91, 284
BssECI CCNNGG 5 cut(s) 113, 450, 486, 693, 774
BssMI GATC 4 cut(s) 357, 445, 826, 1032
BssNI GRCGYC 3 cut(s) 255, 282, 288
BssT1I CCWWGG 3 cut(s) 450, 486, 693
Bst2UI CCWGG 4 cut(s) 173, 299, 775, 1307
Bst4CI ACNGT 1 cut(s) 919
BstACI GRCGYC 3 cut(s) 255, 282, 288
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 7 cut(s) 28, 44, 57, 93, 286, 597, 896
BstF5I GGATG 8 cut(s) 334, 358, 595, 619, 786, 859, 1018, 1352
BstH2I RGCGCY 2 cut(s) 285, 1120
BstHHI GCGC 2 cut(s) 284, 1119
BstKTI GATC 4 cut(s) 360, 448, 829, 1035
BstMAI GTCTC 2 cut(s) 1198, 1301
BstMBI GATC 4 cut(s) 357, 445, 826, 1032
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 4 cut(s) 52, 101, 370, 1106
BstNI CCWGG 4 cut(s) 173, 299, 775, 1307
BstNSI RCATGY 1 cut(s) 410
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 5 cut(s) 66, 171, 297, 773, 1305
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 4 cut(s) 385, 758, 1093, 1356
BstV2I GAAGAC 1 cut(s) 933
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 5 cut(s) 26, 95, 104, 137, 246
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 8 cut(s) 334, 358, 595, 619, 786, 859, 1018, 1352
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 3 cut(s) 198, 756, 1270
Cac8I GCNNGC 7 cut(s) 28, 44, 57, 93, 286, 597, 896
CaiI CAGNNNCTG 1 cut(s) 1306
CfoI GCGC 2 cut(s) 284, 1119
Cfr10I RCCGGY 2 cut(s) 91, 284
Cfr13I GGNCC 9 cut(s) 4, 25, 102, 103, 118, 244, 249, 301, 1192
CpoI CGGWCCG 1 cut(s) 249
CseI GACGC 3 cut(s) 222, 277, 547
Csp6I GTAC 4 cut(s) 130, 315, 337, 1091
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 5 cut(s) 127, 407, 794, 1154, 1315
CviQI GTAC 4 cut(s) 130, 315, 337, 1091
DinI GGCGCC 1 cut(s) 283
DpnI GATC 4 cut(s) 359, 447, 828, 1034
DpnII GATC 4 cut(s) 357, 445, 826, 1032
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 2 cut(s) 93, 135
EagI CGGCCG 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 93
Eco130I CCWWGG 3 cut(s) 450, 486, 693
Eco24I GRGCYC 3 cut(s) 106, 171, 837
Eco31I GGTCTC 1 cut(s) 1198
Eco47I GGWCC 5 cut(s) 4, 118, 249, 301, 1192
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 4 cut(s) 590, 828, 966, 996
Eco72I CACGTG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 112, 431
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 3 cut(s) 102, 103, 1192
EcoRI GAATTC 1 cut(s) 519
EcoRII CCWGG 4 cut(s) 171, 297, 773, 1305
EcoT14I CCWWGG 3 cut(s) 450, 486, 693
EcoT22I ATGCAT 2 cut(s) 408, 583
EcoT38I GRGCYC 3 cut(s) 106, 171, 837
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 3 cut(s) 450, 486, 693
FaeI CATG 5 cut(s) 130, 410, 797, 1157, 1318
FaqI GGGAC 2 cut(s) 413, 1323
FatI CATG 5 cut(s) 126, 406, 793, 1153, 1314
FauI CCCGC 2 cut(s) 35, 843
FauNDI CATATG 1 cut(s) 1102
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 1060
Fnu4HI GCNGC 5 cut(s) 234, 374, 747, 1107, 1345
FokI GGATG 8 cut(s) 341, 365, 602, 606, 793, 866, 1025, 1339
FriOI GRGCYC 3 cut(s) 106, 171, 837
Fsp4HI GCNGC 5 cut(s) 234, 374, 747, 1107, 1345
FspBI CTAG 1 cut(s) 1335
GlaI GCGC 2 cut(s) 283, 1118
GluI GCNGC 5 cut(s) 234, 374, 747, 1107, 1345
GsaI CCCAGC 2 cut(s) 310, 1344
GsuI CTGGAG 3 cut(s) 281, 1283, 1328
HaeII RGCGCY 2 cut(s) 285, 1120
HaeIII GGCC 5 cut(s) 26, 95, 104, 137, 246
HapII CCGG 5 cut(s) 68, 92, 180, 247, 285
HgaI GACGC 3 cut(s) 222, 277, 547
HhaI GCGC 2 cut(s) 284, 1119
Hin1I GRCGYC 3 cut(s) 255, 282, 288
Hin1II CATG 5 cut(s) 130, 410, 797, 1157, 1318
Hin6I GCGC 2 cut(s) 282, 1117
HinP1I GCGC 2 cut(s) 282, 1117
HincII GTYRAC 2 cut(s) 292, 478
HindII GTYRAC 2 cut(s) 292, 478
HindIII AAGCTT 2 cut(s) 362, 1142
HinfI GANTC 5 cut(s) 14, 123, 176, 857, 1186
HpaII CCGG 5 cut(s) 68, 92, 180, 247, 285
HphI GGTGA 2 cut(s) 901, 1330
Hpy166II GTNNAC 5 cut(s) 292, 337, 478, 982, 1061
Hpy188I TCNGA 5 cut(s) 122, 393, 469, 609, 870
Hpy188III TCNNGA 7 cut(s) 164, 180, 824, 830, 974, 1190, 1300
Hpy8I GTNNAC 5 cut(s) 292, 337, 478, 982, 1061
Hpy99I CGWCG 5 cut(s) 77, 101, 233, 257, 260
HpyAV CCTTC 5 cut(s) 149, 941, 1046, 1270, 1318
HpyCH4III ACNGT 1 cut(s) 919
HpyCH4IV ACGT 3 cut(s) 228, 255, 421
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 101, 370, 1106
HpySE526I ACGT 3 cut(s) 228, 255, 421
Hsp92I GRCGYC 3 cut(s) 255, 282, 288
Hsp92II CATG 5 cut(s) 130, 410, 797, 1157, 1318
HspAI GCGC 2 cut(s) 282, 1117
KasI GGCGCC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 179
KroI GCCGGC 2 cut(s) 91, 284
KroNI GCCGGC 2 cut(s) 93, 286
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 4 cut(s) 357, 445, 826, 1032
LmnI GCTCC 7 cut(s) 166, 174, 273, 380, 403, 454, 976
Lsp1109I GCAGC 4 cut(s) 385, 758, 1093, 1356
LweI GCATC 3 cut(s) 319, 515, 874
MaeI CTAG 1 cut(s) 1335
MaeII ACGT 3 cut(s) 228, 255, 421
MaeIII GTNAC 4 cut(s) 19, 649, 676, 937
MalI GATC 4 cut(s) 359, 447, 828, 1034
MbiI CCGCTC 1 cut(s) 221
MboI GATC 4 cut(s) 357, 445, 826, 1032
MboII GAAGA 5 cut(s) 578, 622, 938, 1004, 1333
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 4 cut(s) 106, 171, 837, 1135
MlsI TGGCCA 1 cut(s) 137
MluCI AATT 6 cut(s) 193, 519, 635, 784, 816, 1224
MluNI TGGCCA 1 cut(s) 137
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 4 cut(s) 8, 117, 170, 1195
MmeI TCCRAC 2 cut(s) 145, 1163
Mox20I TGGCCA 1 cut(s) 137
Mph1103I ATGCAT 2 cut(s) 408, 583
MreI CGCCGGCG 1 cut(s) 284
MroI TCCGGA 1 cut(s) 179
MroNI GCCGGC 2 cut(s) 91, 284
MroXI GAANNNNTTC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 137
MseI TTAA 7 cut(s) 534, 717, 734, 921, 1160, 1227, 1245
MslI CAYNNNNRTG 2 cut(s) 624, 1291
Msp20I TGGCCA 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 5 cut(s) 68, 92, 180, 247, 285
MspR9I CCNGG 5 cut(s) 68, 173, 299, 775, 1307
Mva1269I GAATGC 3 cut(s) 159, 421, 581
MvaI CCWGG 4 cut(s) 173, 299, 775, 1307
MwoI GCNNNNNNNGC 4 cut(s) 52, 101, 370, 1106
NaeI GCCGGC 2 cut(s) 93, 286
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 1102
NdeII GATC 4 cut(s) 357, 445, 826, 1032
NgoMIV GCCGGC 2 cut(s) 91, 284
NlaIII CATG 5 cut(s) 130, 410, 797, 1157, 1318
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 3 cut(s) 19, 676, 937
NsiI ATGCAT 2 cut(s) 408, 583
NspI RCATGY 1 cut(s) 410
OliI CACNNNNGTG 1 cut(s) 1291
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 3 cut(s) 159, 421, 581
PdiI GCCGGC 2 cut(s) 93, 286
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 857
PflFI GACNNNGTC 1 cut(s) 17
PflMI CCANNNNNTGG 2 cut(s) 640, 1306
PfoI TCCNGGA 2 cut(s) 171, 297
PkrI GCNGC 5 cut(s) 235, 375, 748, 1108, 1346
PleI GAGTC 4 cut(s) 8, 117, 170, 1194
PluTI GGCGCC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
PpsI GAGTC 4 cut(s) 8, 117, 170, 1194
Ppu21I YACGTR 1 cut(s) 422
PpuMI RGGWCCY 1 cut(s) 1192
PshAI GACNNNNGTC 1 cut(s) 254
PshBI ATTAAT 1 cut(s) 1245
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 1192
Psp6I CCWGG 4 cut(s) 171, 297, 773, 1305
PspCI CACGTG 1 cut(s) 422
PspFI CCCAGC 2 cut(s) 306, 1340
PspGI CCWGG 4 cut(s) 171, 297, 773, 1305
PspOMI GGGCCC 1 cut(s) 102
PspPI GGNCC 9 cut(s) 4, 25, 102, 103, 118, 244, 249, 301, 1192
PspPPI RGGWCCY 1 cut(s) 1192
PstNI CAGNNNCTG 1 cut(s) 1306
PsuI RGATCY 1 cut(s) 826
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 4 cut(s) 131, 316, 338, 1092
RsaNI GTAC 4 cut(s) 130, 315, 337, 1091
RseI CAYNNNNRTG 2 cut(s) 624, 1291
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 7 cut(s) 534, 717, 734, 921, 1160, 1227, 1245
SatI GCNGC 5 cut(s) 234, 374, 747, 1107, 1345
Sau3AI GATC 4 cut(s) 357, 445, 826, 1032
Sau96I GGNCC 9 cut(s) 4, 25, 102, 103, 118, 244, 249, 301, 1192
SchI GAGTC 4 cut(s) 8, 117, 170, 1195
ScrFI CCNGG 5 cut(s) 68, 173, 299, 775, 1307
SduI GDGCHC 4 cut(s) 106, 171, 837, 1135
SfaNI GCATC 3 cut(s) 319, 515, 874
SfiI GGCCNNNNNGGCC 1 cut(s) 101
SfoI GGCGCC 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 5 cut(s) 4, 118, 249, 301, 1192
SmiMI CAYNNNNRTG 2 cut(s) 624, 1291
SmlI CTYRAG 2 cut(s) 542, 830
SmoI CTYRAG 2 cut(s) 542, 830
Sse9I AATT 6 cut(s) 193, 519, 635, 784, 816, 1224
SsiI CCGC 8 cut(s) 7, 28, 219, 234, 304, 326, 332, 850
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 1335
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 5 cut(s) 66, 171, 297, 773, 1305
StyI CCWWGG 3 cut(s) 450, 486, 693
TaaI ACNGT 1 cut(s) 919
TaiI ACGT 3 cut(s) 231, 258, 424
TaqI TCGA 3 cut(s) 87, 823, 1261
TaqII GACCGA 1 cut(s) 266
TasI AATT 6 cut(s) 193, 519, 635, 784, 816, 1224
TatI WGTACW 2 cut(s) 129, 336
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 1 cut(s) 857
Tru1I TTAA 7 cut(s) 534, 717, 734, 921, 1160, 1227, 1245
Tru9I TTAA 7 cut(s) 534, 717, 734, 921, 1160, 1227, 1245
TscAI CASTG 3 cut(s) 205, 756, 1277
TseFI GTSAC 3 cut(s) 19, 676, 937
TseI GCWGC 4 cut(s) 373, 746, 1106, 1344
Tsp45I GTSAC 3 cut(s) 19, 676, 937
TspDTI ATGAA 6 cut(s) 579, 797, 809, 1005, 1011, 1341
TspGWI ACGGA 3 cut(s) 7, 393, 695
TspRI CASTG 3 cut(s) 205, 756, 1277
Tth111I GACNNNGTC 1 cut(s) 17
Van91I CCANNNNNTGG 2 cut(s) 640, 1306
VpaK11BI GGWCC 5 cut(s) 4, 118, 249, 301, 1192
VspI ATTAAT 1 cut(s) 1245
XapI RAATTY 1 cut(s) 519
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 1060
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 1 cut(s) 1335
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 2 cut(s) 408, 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.