RchiOBHm_Chr2g0156981

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019387
Physical Location & Seq
Reverse (-)
73490800 .. 73491363
564 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52574

Sequence Viewer

Length: 564 bp
ATGGAGCGTGTTGGACTTCTGGCAATCTTTAGGTTCGGAACTCCAGCATGCAAAGGAATGGGGGACGGATACTTTCTTAAATCCACAAACGTTCCTGGTTCTGTGGTAGGCGTTGTTGGTAACCCAAATGGCGACTACACTTGTTGGGAAAGACCTGAAGACATGGACACTCCTCGAACGTCCTACATAATAACAAAACAAAATCCAGGCTCTGAAGTCTCGGCCGAAACTGCAGCCGCCCTTGCAGCTTCTTCCATGGTGTTTAGAGGTTCGGACAACAGGTACTCGGCTTTGCTTCTTAACCGAGCCATAGAGGTTTTCGAGTTTGCAGACAAGTATAGAGGATCTTATAACAATAGCATTCCCAATGGAGCGTGCCCGTTTTATTGCGACTACAATGGATACATGGATGAGTTGGTTTGGGGAGCTACGTGGTTGTACAAGGCAACTAAGAGACCTTACTACTGGGGCTATGTCAAACACAACATACATAATTTAGGTCGCGACGTTACTCAGTTTGGATGGGATGTAAAGAATGTTGGCATTCACGTACTCTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

187

Amino Acids

20.86

Weight (kDa)

6.81

Isoelectric Point (pI)

26.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 24 - 185 3.4e-49 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccII CGCG 1 cut(s) 504
AciI CCGC 1 cut(s) 237
AclI AACGTT 1 cut(s) 90
AclWI GGATC 1 cut(s) 352
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 2 cut(s) 177, 234
AfaI GTAC 3 cut(s) 284, 440, 552
AjnI CCWGG 2 cut(s) 94, 205
AluBI AGCT 2 cut(s) 248, 428
AluI AGCT 2 cut(s) 248, 428
Alw26I GTCTC 2 cut(s) 223, 448
AlwI GGATC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 233, 245
BaeGI GKGCMC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 2 cut(s) 245, 257
BccI CCATC 1 cut(s) 516
BciT130I CCWGG 2 cut(s) 96, 207
BciVI GTATCC 2 cut(s) 62, 395
BcoDI GTCTC 2 cut(s) 223, 448
BfmI CTRYAG 1 cut(s) 231
BfuI GTATCC 2 cut(s) 62, 395
BisI GCNGC 3 cut(s) 234, 237, 246
BlsI GCNGC 3 cut(s) 235, 238, 247
Bme1390I CCNGG 2 cut(s) 96, 207
BmrFI CCNGG 2 cut(s) 96, 207
BmrI ACTGGG 1 cut(s) 475
BmuI ACTGGG 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 27
BsaAI YACGTR 2 cut(s) 432, 550
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 470
BseBI CCWGG 2 cut(s) 96, 207
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 3 cut(s) 415, 527, 532
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 2 cut(s) 245, 257
Bsh1236I CGCG 1 cut(s) 504
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 223, 448
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 2 cut(s) 360, 543
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 1 cut(s) 380
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 1 cut(s) 344
Bsp19I CCATGG 1 cut(s) 255
Bsp68I TCGCGA 1 cut(s) 504
BspACI CCGC 1 cut(s) 237
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 96, 207
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 2 cut(s) 432, 550
BstC8I GCNNGC 2 cut(s) 49, 376
BstDEI CTNAG 2 cut(s) 450, 513
BstDSI CCRYGG 1 cut(s) 255
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 3 cut(s) 415, 527, 532
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 1 cut(s) 347
BstMAI GTCTC 2 cut(s) 223, 448
BstMBI GATC 1 cut(s) 344
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 3 cut(s) 230, 242, 245
BstNI CCWGG 2 cut(s) 96, 207
BstNSI RCATGY 1 cut(s) 51
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 94, 205
BstSFI CTRYAG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 380
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 2 cut(s) 245, 257
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
BstZI CGGCCG 1 cut(s) 222
BsuI GTATCC 2 cut(s) 62, 395
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 3 cut(s) 415, 527, 532
BtuMI TCGCGA 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 49, 376
CaiI CAGNNNCTG 1 cut(s) 212
Csp6I GTAC 3 cut(s) 283, 439, 551
CviAII CATG 4 cut(s) 48, 163, 256, 406
CviJI RGCY 8 cut(s) 210, 224, 236, 248, 290, 308, 428, 471
CviKI_1 RGCY 8 cut(s) 210, 224, 236, 248, 290, 308, 428, 471
CviQI GTAC 3 cut(s) 283, 439, 551
DdeI CTNAG 2 cut(s) 450, 513
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 448
Eco52I CGGCCG 1 cut(s) 222
Eco57I CTGAAG 2 cut(s) 177, 234
Eco91I GGTNACC 1 cut(s) 119
EcoO65I GGTNACC 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 94, 205
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 51, 166, 259, 409
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 47, 162, 255, 405
Fnu4HI GCNGC 3 cut(s) 234, 237, 246
FokI GGATG 3 cut(s) 422, 534, 539
Fsp4HI GCNGC 3 cut(s) 234, 237, 246
GluI GCNGC 3 cut(s) 234, 237, 246
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 224
Hin1II CATG 4 cut(s) 51, 166, 259, 409
Hpy188I TCNGA 3 cut(s) 38, 214, 274
Hpy188III TCNNGA 1 cut(s) 503
Hpy99I CGWCG 1 cut(s) 509
HpyCH4IV ACGT 5 cut(s) 90, 179, 431, 507, 549
HpyCH4V TGCA 4 cut(s) 51, 233, 245, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 242, 245
HpyF3I CTNAG 2 cut(s) 450, 513
HpySE526I ACGT 5 cut(s) 90, 179, 431, 507, 549
Hsp92II CATG 4 cut(s) 51, 166, 259, 409
Kzo9I GATC 1 cut(s) 344
LmnI GCTCC 3 cut(s) 4, 371, 425
LpnPI CCDG 9 cut(s) 5, 57, 81, 108, 168, 192, 219, 265, 451
Lsp1109I GCAGC 2 cut(s) 245, 257
MaeII ACGT 5 cut(s) 90, 179, 431, 507, 549
MaeIII GTNAC 2 cut(s) 119, 508
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 3 cut(s) 170, 243, 549
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 1 cut(s) 493
MnlI CCTC 4 cut(s) 183, 260, 307, 335
MseI TTAA 3 cut(s) 78, 300, 562
MspR9I CCNGG 2 cut(s) 96, 207
Mva1269I GAATGC 2 cut(s) 360, 543
MvaI CCWGG 2 cut(s) 96, 207
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 230, 242, 245
NcoI CCATGG 1 cut(s) 255
NdeII GATC 1 cut(s) 344
NlaIII CATG 4 cut(s) 51, 166, 259, 409
NmeAIII GCCGAG 2 cut(s) 200, 266
NruI TCGCGA 1 cut(s) 504
NspI RCATGY 1 cut(s) 51
PaeI GCATGC 1 cut(s) 51
PctI GAATGC 2 cut(s) 360, 543
PkrI GCNGC 3 cut(s) 235, 238, 247
Ppu21I YACGTR 2 cut(s) 432, 550
PsiI TTATAA 1 cut(s) 351
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 94, 205
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 94, 205
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 1 cut(s) 344
RruI TCGCGA 1 cut(s) 504
RsaI GTAC 3 cut(s) 284, 440, 552
RsaNI GTAC 3 cut(s) 283, 439, 551
SaqAI TTAA 3 cut(s) 78, 300, 562
SatI GCNGC 3 cut(s) 234, 237, 246
Sau3AI GATC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 96, 207
SduI GDGCHC 1 cut(s) 380
SfcI CTRYAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 51
Sse9I AATT 1 cut(s) 493
SsiI CCGC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 94, 205
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 5 cut(s) 93, 182, 434, 510, 552
TaqI TCGA 2 cut(s) 175, 321
TasI AATT 1 cut(s) 493
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 239
Tru1I TTAA 3 cut(s) 78, 300, 562
Tru9I TTAA 3 cut(s) 78, 300, 562
TseI GCWGC 2 cut(s) 233, 245
TspGWI ACGGA 1 cut(s) 81
XceI RCATGY 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.