Rh2DG545400

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0019387
Physical Location & Seq
Reverse (-)
77446847 .. 77447116
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG545400.1

Sequence Viewer

Length: 270 bp
ATGGAGCGTGTTGGAGTTCTGGCAATCTTTAGGTTTGGAACTCCAGCATGCAAAGGAATGGGGGACGGATACTTTCTTAAATCCACAAACGTTCCTGGTTCCGTGGTAGGCGTTGTTGGTAACCCAAATGGCGACTACACTTGTTGGGAAAGACCCGAAGACATGGACACTCCTCGAAAGTCCTACATAGTAACAAAACAAAAGCCAGGCTCTGAAGTCTCGGCCGAAACTGCAGTCGCCCTTGCAGCTAGCTTCTTCCATGGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

89

Amino Acids

9.5

Weight (kDa)

6.54

Isoelectric Point (pI)

14.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 24 - 84 2e-14 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 90
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 234
AjnI CCWGG 2 cut(s) 94, 205
AluBI AGCT 2 cut(s) 248, 252
AluI AGCT 2 cut(s) 248, 252
Alw26I GTCTC 1 cut(s) 223
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AsuNHI GCTAGC 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 1 cut(s) 257
BciT130I CCWGG 2 cut(s) 96, 207
BciVI GTATCC 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 1 cut(s) 249
BfmI CTRYAG 1 cut(s) 231
BfuI GTATCC 1 cut(s) 62
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 96, 207
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 2 cut(s) 96, 207
BmtI GCTAGC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 102, 259
BseBI CCWGG 2 cut(s) 96, 207
BseDI CCNNGG 2 cut(s) 102, 259
BseRI GAGGAG 1 cut(s) 162
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 225
BslFI GGGAC 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 224
Bsp19I CCATGG 1 cut(s) 259
BspANI GGCC 1 cut(s) 224
BspLI GGNNCC 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 235
BspOI GCTAGC 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 102, 259
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 2 cut(s) 96, 207
BstC8I GCNNGC 2 cut(s) 49, 250
BstDSI CCRYGG 2 cut(s) 102, 259
BstEII GGTNACC 1 cut(s) 119
BstMAI GTCTC 1 cut(s) 223
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 230, 245
BstNI CCWGG 2 cut(s) 96, 207
BstNSI RCATGY 1 cut(s) 51
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 94, 205
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 257
BstV2I GAAGAC 1 cut(s) 165
BstZI CGGCCG 1 cut(s) 222
BsuI GTATCC 1 cut(s) 62
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 2 cut(s) 102, 259
Cac8I GCNNGC 2 cut(s) 49, 250
CaiI CAGNNNCTG 1 cut(s) 212
CviAII CATG 3 cut(s) 48, 163, 260
CviJI RGCY 5 cut(s) 205, 210, 224, 248, 252
CviKI_1 RGCY 5 cut(s) 205, 210, 224, 248, 252
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 259
Eco52I CGGCCG 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 119
EcoO65I GGTNACC 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 94, 205
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 3 cut(s) 51, 166, 263
FaiI YATR 4 cut(s) 49, 164, 188, 261
FaqI GGGAC 1 cut(s) 77
FatI CATG 3 cut(s) 47, 162, 259
Fnu4HI GCNGC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 1 cut(s) 249
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 224
Hin1II CATG 3 cut(s) 51, 166, 263
Hpy188I TCNGA 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 51, 233, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 245
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 3 cut(s) 51, 166, 263
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 6 cut(s) 5, 57, 81, 108, 192, 219
Lsp1109I GCAGC 1 cut(s) 257
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 119, 190
MboII GAAGA 2 cut(s) 170, 247
MnlI CCTC 1 cut(s) 183
MseI TTAA 1 cut(s) 78
MspR9I CCNGG 2 cut(s) 96, 207
MvaI CCWGG 2 cut(s) 96, 207
MwoI GCNNNNNNNGC 2 cut(s) 230, 245
NcoI CCATGG 1 cut(s) 259
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 3 cut(s) 51, 166, 263
NlaIV GGNNCC 1 cut(s) 100
NmeAIII GCCGAG 1 cut(s) 200
NspI RCATGY 1 cut(s) 51
PaeI GCATGC 1 cut(s) 51
PkrI GCNGC 1 cut(s) 247
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 94, 205
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 94, 205
PspN4I GGNNCC 1 cut(s) 100
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 212
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 246
ScrFI CCNGG 2 cut(s) 96, 207
SetI ASST 4 cut(s) 35, 93, 250, 254
SfcI CTRYAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 51
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 2 cut(s) 94, 205
StyI CCWWGG 1 cut(s) 259
TaiI ACGT 1 cut(s) 93
TaqI TCGA 1 cut(s) 175
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 1 cut(s) 245
TspGWI ACGGA 2 cut(s) 81, 91
XceI RCATGY 1 cut(s) 51
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.