RLG00000020956

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019387
Physical Location & Seq
Reverse (-)
70482702 .. 70483274
573 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020956

Sequence Viewer

Length: 462 bp
ATGTTGGCCATGGCAACATTCACAAGCTCTGAAGGGCATTCGCAAAACTACGCGGATGCTTTGTCGAAGAGTATTTTGTTTTCCGAAGGACAGAGGTCGGAAGTTCAACTTTCCCATGGCCTTCACAACAACAATGTGGTCATGGAGCGTGTTGGAGTTCTGGCAATCTTTAGGTTTGGAACTCCAGCATGCAAAGGAATGGGGGACGGATACTTTCTTAAATCCACAAACGTTCCTGGTTCCGTGGTAGGCGTTGTTGGTAACCCAAATGGCGACTACACTTGTTGGGAAAGACCCGAAGACATGGACACTCCTCGAAAGTCCTACATAGTAACAAAACAAAAGCCAGGCTCTGAAGTCTCGGCCGAAACTGCAGCCGCCCTTGCAGCTTCTTCCATGGTGTTTAGAGGTTCGGACAACAGGTCAACAGGTACTCGGCTTTGCTTCTTAACCGAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

154

Amino Acids

16.43

Weight (kDa)

6.28

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 71 - 140 2.6e-19 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 53
AciI CCGC 2 cut(s) 53, 378
AclI AACGTT 1 cut(s) 231
AcoI YGGCCR 2 cut(s) 6, 363
AcuI CTGAAG 2 cut(s) 51, 375
AfaI GTAC 1 cut(s) 433
AgsI TTSAA 1 cut(s) 107
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 235, 346
AluBI AGCT 2 cut(s) 27, 389
AluI AGCT 2 cut(s) 27, 389
Alw26I GTCTC 1 cut(s) 364
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 3 cut(s) 6, 118, 363
ApeKI GCWGC 2 cut(s) 374, 386
BalI TGGCCA 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 386, 398
BciT130I CCWGG 2 cut(s) 237, 348
BciVI GTATCC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 364
BfmI CTRYAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 203
BisI GCNGC 3 cut(s) 375, 378, 387
BlsI GCNGC 3 cut(s) 376, 379, 388
Bme1390I CCNGG 2 cut(s) 237, 348
BmeRI GACNNNNNGTC 1 cut(s) 421
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 2 cut(s) 237, 348
BmsI GCATC 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 168
BsaJI CCNNGG 4 cut(s) 9, 115, 243, 396
BseBI CCWGG 2 cut(s) 237, 348
BseDI CCNNGG 4 cut(s) 9, 115, 243, 396
BseGI GGATG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 303
BseX3I CGGCCG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 386, 398
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 366
BshFI GGCC 3 cut(s) 8, 120, 365
BsiEI CGRYCG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 3 cut(s) 8, 120, 365
Bsp19I CCATGG 3 cut(s) 9, 115, 396
BspACI CCGC 2 cut(s) 53, 378
BspANI GGCC 3 cut(s) 8, 120, 365
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 376
BssECI CCNNGG 4 cut(s) 9, 115, 243, 396
BssT1I CCWWGG 3 cut(s) 9, 115, 396
Bst2UI CCWGG 2 cut(s) 237, 348
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 190
BstDSI CCRYGG 4 cut(s) 9, 115, 243, 396
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 1 cut(s) 61
BstFNI CGCG 1 cut(s) 53
BstMAI GTCTC 1 cut(s) 364
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 371, 383, 386
BstNI CCWGG 2 cut(s) 237, 348
BstNSI RCATGY 1 cut(s) 192
BstPAI GACNNNNGTC 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 235, 346
BstSFI CTRYAG 1 cut(s) 372
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 386, 398
BstV2I GAAGAC 1 cut(s) 306
BstZI CGGCCG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 3 cut(s) 8, 120, 365
BtgI CCRYGG 4 cut(s) 9, 115, 243, 396
BtsCI GGATG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 190
CaiI CAGNNNCTG 1 cut(s) 353
Csp6I GTAC 1 cut(s) 432
CviAII CATG 6 cut(s) 10, 116, 142, 189, 304, 397
CviQI GTAC 1 cut(s) 432
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 2 cut(s) 6, 363
EagI CGGCCG 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 62
EclXI CGGCCG 1 cut(s) 363
Eco130I CCWWGG 3 cut(s) 9, 115, 396
Eco52I CGGCCG 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 51, 375
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 235, 346
EcoT14I CCWWGG 3 cut(s) 9, 115, 396
ErhI CCWWGG 3 cut(s) 9, 115, 396
FaeI CATG 6 cut(s) 13, 119, 145, 192, 307, 400
FaiI YATR 8 cut(s) 11, 117, 143, 190, 305, 329, 398, 460
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 9, 115, 141, 188, 303, 396
Fnu4HI GCNGC 3 cut(s) 375, 378, 387
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 3 cut(s) 375, 378, 387
GluI GCNGC 3 cut(s) 375, 378, 387
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 3 cut(s) 8, 120, 365
Hin1II CATG 6 cut(s) 13, 119, 145, 192, 307, 400
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 5 cut(s) 31, 85, 100, 355, 415
Hpy8I GTNNAC 1 cut(s) 426
HpyAV CCTTC 3 cut(s) 26, 80, 131
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 192, 374, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 383, 386
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 6 cut(s) 13, 119, 145, 192, 307, 400
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 8 cut(s) 146, 198, 222, 249, 333, 360, 406, 414
Lsp1109I GCAGC 2 cut(s) 386, 398
LweI GCATC 1 cut(s) 46
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 260, 331
MboII GAAGA 3 cut(s) 79, 311, 384
MlsI TGGCCA 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 78, 133
MnlI CCTC 3 cut(s) 87, 324, 401
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 2 cut(s) 219, 449
Msp20I TGGCCA 1 cut(s) 8
MspR9I CCNGG 2 cut(s) 237, 348
Mva1269I GAATGC 1 cut(s) 37
MvaI CCWGG 2 cut(s) 237, 348
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 371, 383, 386
NcoI CCATGG 3 cut(s) 9, 115, 396
NlaIII CATG 6 cut(s) 13, 119, 145, 192, 307, 400
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 2 cut(s) 341, 415
NspI RCATGY 1 cut(s) 192
PaeI GCATGC 1 cut(s) 192
PctI GAATGC 1 cut(s) 37
PkrI GCNGC 3 cut(s) 376, 379, 388
PshAI GACNNNNGTC 1 cut(s) 94
Psp1406I AACGTT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 235, 346
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 2 cut(s) 235, 346
PspN4I GGNNCC 1 cut(s) 241
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
SaqAI TTAA 2 cut(s) 219, 449
SatI GCNGC 3 cut(s) 375, 378, 387
ScrFI CCNGG 2 cut(s) 237, 348
SetI ASST 8 cut(s) 29, 98, 176, 234, 391, 412, 425, 433
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 372
SphI GCATGC 1 cut(s) 192
SsiI CCGC 2 cut(s) 53, 378
StyD4I CCNGG 2 cut(s) 235, 346
StyI CCWWGG 3 cut(s) 9, 115, 396
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 65, 316
TauI GCSGC 1 cut(s) 380
Tru1I TTAA 2 cut(s) 219, 449
Tru9I TTAA 2 cut(s) 219, 449
TseI GCWGC 2 cut(s) 374, 386
TspGWI ACGGA 2 cut(s) 222, 232
XceI RCATGY 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.