Rh2AG523200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019387
Physical Location & Seq
Reverse (-)
75036046 .. 75036873
828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG523200.1

Sequence Viewer

Length: 828 bp
ATGGAGCGTGTTGGAGTTCTGGCAATCTTTAGGTTCGGAACTCCAGCATGCAAAGGAATGGGGGACGGATACTTTCTTAAATTCACAAACGTTCCTGGTTCTGTGGTAGGCGTTGTTGGTAACCCAAATGGCGACTACACTTGTTGGGAAAGACCCGAAGACATGGACACTCCTCGAACGTCCTACATAGTAACAAAACAAAATCCAGGCTCTGAAGTCTCGGCCGAAACTGCAGCCGCCCTTGCAGCTTCTTCCATGGTGTTTAGAGGTTCGGACAACAGGTACTCGGCTTTGCTTCTTAACCGAGCCATAGAGGTTTTCGAGTTTGCAGACAAGTATAGAGGATCTTATAACAATAGCATTCCCAATGGAGCGTGCCCGTTTTATTGCGACTACAATGGATACATGGATGAGTTGGTTTGGGGAGCTACGTGGTTGTACAAGGCAACAAAGAGACCTTACTACTGGAGCTATGTCAAACACAACATACATAATTTAGGTAGCGACGTTACTCAGTTTGGATGGGATGCAAAGAATGCTGGCATTCACGTACTCTCTTCTCAATGGGTAATGTCTCGTAATGTTCCCTCCAAGACAGACTTGCTCGTATGCTCGATTTTGCCCGAATCACATGCCCCAAAATCAGTCTCCTATACGCGGGGCGGGCTCTTCTTTAAAAGTGGAGGAAGCAACATGCAACATACGACTTCATTATCGTTTCTTCTTACGGTTTATGCTAACTACATAAAAGCCACCAATAGAGTGGTTAACTGCGGCAACAACGTCGTGATCACTCCGGCTAGGCTGGTCAATTTTGCCAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

275

Amino Acids

30.48

Weight (kDa)

9.04

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 24 - 261 2.4e-59 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccII CGCG 1 cut(s) 658
AciI CCGC 4 cut(s) 237, 658, 663, 774
AclI AACGTT 1 cut(s) 90
AclWI GGATC 1 cut(s) 352
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 3 cut(s) 284, 440, 552
AfiI CCNNNNNNNGG 1 cut(s) 657
AjnI CCWGG 2 cut(s) 94, 205
AluBI AGCT 3 cut(s) 248, 428, 471
AluI AGCT 3 cut(s) 248, 428, 471
Alw26I GTCTC 4 cut(s) 223, 448, 579, 652
AlwI GGATC 1 cut(s) 352
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 233, 245
ApoI RAATTY 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 165
BbvI GCAGC 2 cut(s) 245, 257
BccI CCATC 1 cut(s) 516
BcgI CGANNNNNNTGC 4 cut(s) 614, 648, 766, 800
BciT130I CCWGG 2 cut(s) 96, 207
BciVI GTATCC 2 cut(s) 62, 395
BclI TGATCA 1 cut(s) 789
BcoDI GTCTC 4 cut(s) 223, 448, 579, 652
BfaI CTAG 1 cut(s) 801
BfmI CTRYAG 1 cut(s) 231
BfuI GTATCC 2 cut(s) 62, 395
BisI GCNGC 4 cut(s) 234, 237, 246, 775
BlsI GCNGC 4 cut(s) 235, 238, 247, 776
Bme1390I CCNGG 2 cut(s) 96, 207
BmrFI CCNGG 2 cut(s) 96, 207
BmsI GCATC 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 2 cut(s) 27, 487
BsaAI YACGTR 2 cut(s) 432, 550
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 657
Bse1I ACTGG 1 cut(s) 470
BseBI CCWGG 2 cut(s) 96, 207
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 3 cut(s) 415, 527, 532
BseLI CCNNNNNNNGG 1 cut(s) 657
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 1 cut(s) 470
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 222
BseXI GCAGC 2 cut(s) 245, 257
Bsh1236I CGCG 1 cut(s) 658
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 224
BsiEI CGRYCG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 797
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 657
BsmAI GTCTC 4 cut(s) 223, 448, 579, 652
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 3 cut(s) 360, 541, 543
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 2 cut(s) 380, 669
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 2 cut(s) 344, 789
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 4 cut(s) 237, 658, 663, 774
BspANI GGCC 1 cut(s) 224
BspCNI CTCAG 1 cut(s) 526
BspFNI CGCG 1 cut(s) 658
BspMAI CTGCAG 1 cut(s) 235
BspPI GGATC 1 cut(s) 352
BspQI GCTCTTC 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 448
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 344, 789
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 96, 207
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 2 cut(s) 562, 674
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 2 cut(s) 432, 550
BstC8I GCNNGC 4 cut(s) 49, 376, 541, 665
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 255
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 3 cut(s) 415, 527, 532
BstFNI CGCG 1 cut(s) 658
BstKTI GATC 2 cut(s) 347, 792
BstMAI GTCTC 4 cut(s) 223, 448, 579, 652
BstMBI GATC 2 cut(s) 344, 789
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 5 cut(s) 230, 242, 245, 536, 664
BstNI CCWGG 2 cut(s) 96, 207
BstNSI RCATGY 3 cut(s) 51, 635, 697
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 94, 205
BstSFI CTRYAG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 380
BstUI CGCG 1 cut(s) 658
BstV1I GCAGC 2 cut(s) 245, 257
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 1 cut(s) 344
BstZI CGGCCG 1 cut(s) 222
BsuI GTATCC 2 cut(s) 62, 395
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 3 cut(s) 415, 527, 532
Cac8I GCNNGC 4 cut(s) 49, 376, 541, 665
CaiI CAGNNNCTG 1 cut(s) 212
Csp6I GTAC 3 cut(s) 283, 439, 551
CviAII CATG 6 cut(s) 48, 163, 256, 406, 632, 694
CviQI GTAC 3 cut(s) 283, 439, 551
DdeI CTNAG 1 cut(s) 513
DpnI GATC 2 cut(s) 346, 791
DpnII GATC 2 cut(s) 344, 789
DraI TTTAAA 1 cut(s) 676
EaeI YGGCCR 1 cut(s) 222
EagI CGGCCG 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 562, 674
EarI CTCTTC 2 cut(s) 562, 674
EclXI CGGCCG 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 448
Eco52I CGGCCG 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 119
EcoO65I GGTNACC 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 94, 205
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 6 cut(s) 51, 166, 259, 409, 635, 697
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 77
FatI CATG 6 cut(s) 47, 162, 255, 405, 631, 693
FauI CCCGC 2 cut(s) 651, 656
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 4 cut(s) 234, 237, 246, 775
FokI GGATG 3 cut(s) 422, 534, 539
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 4 cut(s) 234, 237, 246, 775
FspBI CTAG 1 cut(s) 801
GluI GCNGC 4 cut(s) 234, 237, 246, 775
GsuI CTGGAG 2 cut(s) 27, 487
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 797
Hin1II CATG 6 cut(s) 51, 166, 259, 409, 635, 697
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 1 cut(s) 626
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 1 cut(s) 797
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 38, 214, 274
Hpy188III TCNNGA 1 cut(s) 787
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 2 cut(s) 509, 788
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 6 cut(s) 90, 179, 431, 507, 549, 783
HpyCH4V TGCA 6 cut(s) 51, 233, 245, 329, 530, 697
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 242, 245, 536, 664
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 6 cut(s) 90, 179, 431, 507, 549, 783
Hsp92II CATG 6 cut(s) 51, 166, 259, 409, 635, 697
Ksp22I TGATCA 1 cut(s) 789
KspAI GTTAAC 1 cut(s) 769
Kzo9I GATC 2 cut(s) 344, 789
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 4 cut(s) 4, 371, 425, 468
Lsp1109I GCAGC 2 cut(s) 245, 257
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 801
MaeII ACGT 6 cut(s) 90, 179, 431, 507, 549, 783
MaeIII GTNAC 3 cut(s) 119, 190, 508
MalI GATC 2 cut(s) 346, 791
MboI GATC 2 cut(s) 344, 789
MboII GAAGA 5 cut(s) 170, 243, 549, 661, 713
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 2 cut(s) 380, 669
MluCI AATT 3 cut(s) 80, 493, 811
MnlI CCTC 6 cut(s) 183, 260, 307, 335, 598, 677
MseI TTAA 4 cut(s) 78, 300, 675, 768
MspI CCGG 1 cut(s) 797
MspR9I CCNGG 2 cut(s) 96, 207
Mva1269I GAATGC 3 cut(s) 360, 541, 543
MvaI CCWGG 2 cut(s) 96, 207
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 5 cut(s) 230, 242, 245, 536, 664
NcoI CCATGG 1 cut(s) 255
NdeII GATC 2 cut(s) 344, 789
NlaIII CATG 6 cut(s) 51, 166, 259, 409, 635, 697
NmeAIII GCCGAG 2 cut(s) 200, 266
NspI RCATGY 3 cut(s) 51, 635, 697
PaeI GCATGC 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 674
PctI GAATGC 3 cut(s) 360, 541, 543
PfeI GAWTC 1 cut(s) 626
PkrI GCNGC 4 cut(s) 235, 238, 247, 776
Ppu21I YACGTR 2 cut(s) 432, 550
PsiI TTATAA 1 cut(s) 351
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 94, 205
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 94, 205
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 3 cut(s) 284, 440, 552
RsaNI GTAC 3 cut(s) 283, 439, 551
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 4 cut(s) 78, 300, 675, 768
SatI GCNGC 4 cut(s) 234, 237, 246, 775
Sau3AI GATC 2 cut(s) 344, 789
ScrFI CCNGG 2 cut(s) 96, 207
SduI GDGCHC 2 cut(s) 380, 669
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 51
Sse9I AATT 3 cut(s) 80, 493, 811
SsiI CCGC 4 cut(s) 237, 658, 663, 774
SspMI CTAG 1 cut(s) 801
StyD4I CCNGG 2 cut(s) 94, 205
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 6 cut(s) 93, 182, 434, 510, 552, 786
TaqI TCGA 3 cut(s) 175, 321, 614
TasI AATT 3 cut(s) 80, 493, 811
TatI WGTACW 1 cut(s) 438
TauI GCSGC 2 cut(s) 239, 777
TfiI GAWTC 1 cut(s) 626
Tru1I TTAA 4 cut(s) 78, 300, 675, 768
Tru9I TTAA 4 cut(s) 78, 300, 675, 768
TseI GCWGC 2 cut(s) 233, 245
TspDTI ATGAA 1 cut(s) 699
TspGWI ACGGA 1 cut(s) 81
XapI RAATTY 1 cut(s) 80
XceI RCATGY 3 cut(s) 51, 635, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XspI CTAG 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.