Rorug07G0215500

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0019387
Physical Location & Seq
Reverse (-)
19123053 .. 19123715
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0215500.1

Sequence Viewer

Length: 663 bp
ATGCCCTCCCACGACCATCTGGCCTCCAAATTGGAGAACACTTGCTGCCTAGACGATGTTGAACAAGAGGTGTCTTTACATGGAGCAATAATTGCCAATGTGGTTCCAAATATTGGTGACATCAAGGGATCCCTCAGAAGCTCCTGGAAGGATATAGGAGGCTTCAAAATTGCCTACCTGTACCACAACCTCTTCTCCATCAAAACATCTCCAGAGGGTGCCAAAATCCTGCTAGACGGAGGTCCATGGCACGTCGACAACATGAGGTTCAATGTGGTTAGATGGCTACCCAACCTTACCATAAATGATGTTCACTTTCATAAAGTTTGGTATTGGGTACAAATCTCAGGTCTTACCTATGAGAAAATGAACCCAAAAAATGGTATGCTGATTGGATCAGAAATTGGAACCGTGTTAGAACTTGAGGATGCAGAGAAGGTTGACGCAACCCTAAGAGGTTACCTCAGAGTTAAGGTTTTGCTGGATGCTAGAAACCCTCTACCCACTGGCTTTTGGCTTCCTCTCAATGAGTATACCAAAACCAGAGTAGACTACGCATATGAGGGGCTCTGCGATTTTTGCTACAAATGTGGTCGTCTGGGGCACCACATGGATGTTTGCAAGAATTTCAGGATCGTCAACCCGGATGAAGGCGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

220

Amino Acids

24.99

Weight (kDa)

5.97

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 31 - 100 1.8e-08 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 180 - 208 9.2e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 218, 603
AccB7I CCANNNNNTGG 2 cut(s) 113, 380
AccI GTMKAC 3 cut(s) 255, 533, 549
AclWI GGATC 4 cut(s) 123, 136, 403, 641
AcsI RAATTY 1 cut(s) 625
AfaI GTAC 2 cut(s) 182, 339
AfiI CCNNNNNNNGG 3 cut(s) 113, 380, 650
AgsI TTSAA 3 cut(s) 62, 166, 271
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
AlwI GGATC 4 cut(s) 123, 136, 403, 641
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 1 cut(s) 625
ArsI GACNNNNNNTTYG 2 cut(s) 334, 366
AspS9I GGNCC 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 606
BamHI GGATCC 1 cut(s) 128
BanI GGYRCC 2 cut(s) 218, 603
BanII GRGCYC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 32
BccI CCATC 3 cut(s) 24, 206, 276
BciT130I CCWGG 1 cut(s) 145
BcnI CCSGG 1 cut(s) 644
BfaI CTAG 3 cut(s) 50, 233, 489
BisI GCNGC 1 cut(s) 46
BlsI GCNGC 1 cut(s) 47
Bme1390I CCNGG 2 cut(s) 145, 644
Bme18I GGWCC 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 5 cut(s) 105, 130, 220, 409, 605
BmrFI CCNGG 2 cut(s) 145, 644
BmsI GCATC 2 cut(s) 418, 475
BoxI GACNNNNGTC 1 cut(s) 240
BplI GAGNNNNNCTC 2 cut(s) 447, 479
BpmI CTGGAG 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 644
BsaJI CCNNGG 1 cut(s) 245
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 380, 650
Bse1I ACTGG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 4 cut(s) 433, 490, 619, 652
BseLI CCNNNNNNNGG 3 cut(s) 113, 380, 650
BseMII CTCAG 3 cut(s) 148, 360, 478
BseNI ACTGG 1 cut(s) 511
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 1 cut(s) 32
BshFI GGCC 1 cut(s) 23
BshNI GGYRCC 2 cut(s) 218, 603
BsiSI CCGG 1 cut(s) 644
BslI CCNNNNNNNGG 3 cut(s) 113, 380, 650
BsnI GGCC 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 570, 606
Bsp143I GATC 3 cut(s) 128, 395, 633
Bsp19I CCATGG 1 cut(s) 245
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 3 cut(s) 147, 359, 477
BspLI GGNNCC 5 cut(s) 105, 130, 220, 409, 605
BspPI GGATC 4 cut(s) 123, 136, 403, 641
BspT107I GGYRCC 2 cut(s) 218, 603
BsrI ACTGG 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 3 cut(s) 128, 395, 633
BssNAI GTATAC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 245
Bst1107I GTATAC 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 92
BstDEI CTNAG 4 cut(s) 134, 346, 452, 464
BstDSI CCRYGG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 458
BstF5I GGATG 4 cut(s) 433, 490, 619, 652
BstKTI GATC 3 cut(s) 131, 398, 636
BstMBI GATC 3 cut(s) 128, 395, 633
BstMWI GCNNNNNNNGC 2 cut(s) 92, 579
BstNI CCWGG 1 cut(s) 145
BstPAI GACNNNNGTC 1 cut(s) 240
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 143, 642
BstSLI GKGCMC 1 cut(s) 606
BstV1I GCAGC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BstZ17I GTATAC 1 cut(s) 534
BsuRI GGCC 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 245
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 4 cut(s) 433, 490, 619, 652
BtsIMutI CAGTG 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 181, 338
CviAII CATG 4 cut(s) 80, 246, 262, 610
CviJI RGCY 7 cut(s) 23, 141, 162, 286, 510, 517, 568
CviKI_1 RGCY 7 cut(s) 23, 141, 162, 286, 510, 517, 568
CviQI GTAC 2 cut(s) 181, 338
DdeI CTNAG 4 cut(s) 134, 346, 452, 464
DpnI GATC 3 cut(s) 130, 397, 635
DpnII GATC 3 cut(s) 128, 395, 633
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 4 cut(s) 83, 249, 265, 613
FatI CATG 4 cut(s) 79, 245, 261, 609
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 3 cut(s) 255, 533, 549
Fnu4HI GCNGC 1 cut(s) 46
FokI GGATG 4 cut(s) 440, 497, 626, 659
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 46
FspBI CTAG 3 cut(s) 50, 233, 489
GluI GCNGC 1 cut(s) 46
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 644
HgaI GACGC 1 cut(s) 452
Hin1II CATG 4 cut(s) 83, 249, 265, 613
HincII GTYRAC 3 cut(s) 256, 442, 640
HindII GTYRAC 3 cut(s) 256, 442, 640
HpaII CCGG 1 cut(s) 644
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 6 cut(s) 256, 313, 442, 534, 550, 640
Hpy188I TCNGA 3 cut(s) 137, 400, 467
Hpy188III TCNNGA 2 cut(s) 212, 631
Hpy8I GTNNAC 6 cut(s) 256, 313, 442, 534, 550, 640
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 142, 430, 644
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 431, 621
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 579
HpyF3I CTNAG 4 cut(s) 134, 346, 452, 464
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 4 cut(s) 83, 249, 265, 613
Kzo9I GATC 3 cut(s) 128, 395, 633
LmnI GCTCC 2 cut(s) 83, 146
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 2 cut(s) 418, 475
MaeI CTAG 3 cut(s) 50, 233, 489
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 116, 458
MalI GATC 3 cut(s) 130, 397, 635
MboI GATC 3 cut(s) 128, 395, 633
MboII GAAGA 1 cut(s) 184
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 2 cut(s) 570, 606
MluCI AATT 5 cut(s) 29, 90, 168, 402, 625
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 612
MspI CCGG 1 cut(s) 644
MspR9I CCNGG 2 cut(s) 145, 644
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 2 cut(s) 92, 579
NciI CCSGG 1 cut(s) 644
NcoI CCATGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 559
NdeII GATC 3 cut(s) 128, 395, 633
NlaIII CATG 4 cut(s) 83, 249, 265, 613
NlaIV GGNNCC 5 cut(s) 105, 130, 220, 409, 605
NmuCI GTSAC 1 cut(s) 116
PflMI CCANNNNNTGG 2 cut(s) 113, 380
PfoI TCCNGGA 1 cut(s) 143
PkrI GCNGC 1 cut(s) 47
PshAI GACNNNNGTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 143
PspEI GGTNACC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 5 cut(s) 105, 130, 220, 409, 605
PspPI GGNCC 1 cut(s) 242
PsuI RGATCY 1 cut(s) 128
RsaI GTAC 2 cut(s) 182, 339
RsaNI GTAC 2 cut(s) 181, 338
RseI CAYNNNNRTG 1 cut(s) 612
SalI GTCGAC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 1 cut(s) 46
Sau3AI GATC 3 cut(s) 128, 395, 633
Sau96I GGNCC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 145, 644
SduI GDGCHC 2 cut(s) 570, 606
SfaNI GCATC 2 cut(s) 418, 475
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 5 cut(s) 29, 90, 168, 402, 625
SspI AATATT 1 cut(s) 112
SspMI CTAG 3 cut(s) 50, 233, 489
StyD4I CCNGG 2 cut(s) 143, 642
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 255
TasI AATT 5 cut(s) 29, 90, 168, 402, 625
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 308, 383, 663
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 511
Van91I CCANNNNNTGG 2 cut(s) 113, 380
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 625
XmiI GTMKAC 3 cut(s) 255, 533, 549
XspI CTAG 3 cut(s) 50, 233, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.