RchiOBHm_Chr2g0158841

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019882
Physical Location & Seq
Reverse (-)
75008468 .. 75008863
396 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52751

Sequence Viewer

Length: 396 bp
ATGACATTCAACTTCATGTCGAAGCTCACTCAACAATCCCTACCTATACTACCACTACTAGTTTTGGTTTTTGCCAGAAGTTTAGCTAAAGCTAGTGATCTCAAAGAAACCCAATTAGTCTTGTACTTCCAGGATTTCACTGCAGGTCCAAATACCTCAAGCATACCAGTTGCAGGCATCGCTGGAAAGCTGTGGACCTTCACTCAATTCGGAACAATTTATGTCACCGATGACACCATGACCCAAGGACCGAACATAACATCTTCCGTAGTAGGACGGGCCCACGGCATAACGGTGGCATCGGCACTGGACGGAAGCAATGCCCTTGTCTTGGTGTCTCGTGTTCACAAACATGAAGTACAATGGCAGCACTCTAGAGATACAAGGAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

131

Amino Acids

14.36

Weight (kDa)

9.52

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 37 - 113 3e-17 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019882)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0158841
rosa_multiflora Rmu_sc0014404.1_g000002
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538900 Rh2BG551500 Rh2CG522200 Rh2DG561300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 134
AfaI GTAC 2 cut(s) 125, 360
AfiI CCNNNNNNNGG 2 cut(s) 173, 331
AgsI TTSAA 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 4 cut(s) 25, 86, 92, 190
AluI AGCT 4 cut(s) 25, 86, 92, 190
Alw26I GTCTC 1 cut(s) 342
AoxI GGCC 1 cut(s) 279
ApaI GGGCCC 1 cut(s) 283
ApeKI GCWGC 1 cut(s) 367
AspS9I GGNCC 5 cut(s) 146, 195, 248, 279, 280
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 3 cut(s) 146, 195, 248
BaeGI GKGCMC 1 cut(s) 283
BanII GRGCYC 1 cut(s) 283
BauI CACGAG 1 cut(s) 339
BbvI GCAGC 1 cut(s) 379
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 342
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 3 cut(s) 59, 93, 375
BfmI CTRYAG 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 3 cut(s) 146, 195, 248
BmgT120I GGNCC 5 cut(s) 146, 195, 248, 279, 280
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 186, 308
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 244, 283
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 331
Bse1I ACTGG 2 cut(s) 167, 312
Bse3DI GCAATG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 244, 283
BseLI CCNNNNNNNGG 2 cut(s) 173, 331
BseMI GCAATG 1 cut(s) 325
BseNI ACTGG 2 cut(s) 167, 312
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 173, 331
BsmAI GTCTC 1 cut(s) 342
BsnI GGCC 1 cut(s) 281
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 1 cut(s) 97
BspANI GGCC 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 281
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 325
BsrI ACTGG 2 cut(s) 167, 312
BssECI CCNNGG 2 cut(s) 244, 283
BssMI GATC 1 cut(s) 97
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 244
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 295, 393
BstC8I GCNNGC 1 cut(s) 175
BstDSI CCRYGG 1 cut(s) 283
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstSFI CTRYAG 1 cut(s) 141
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 138, 305
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 175
Cfr13I GGNCC 5 cut(s) 146, 195, 248, 279, 280
Csp6I GTAC 2 cut(s) 124, 359
CviAII CATG 3 cut(s) 16, 238, 353
CviJI RGCY 5 cut(s) 25, 86, 92, 190, 281
CviKI_1 RGCY 5 cut(s) 25, 86, 92, 190, 281
CviQI GTAC 2 cut(s) 124, 359
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 3 cut(s) 146, 195, 248
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 3 cut(s) 19, 241, 356
FaiI YATR 8 cut(s) 17, 47, 164, 222, 239, 257, 290, 354
FatI CATG 3 cut(s) 15, 237, 352
Fnu4HI GCNGC 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 3 cut(s) 59, 93, 375
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 1 cut(s) 281
Hin1II CATG 3 cut(s) 19, 241, 356
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 195, 346
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 1 cut(s) 375
Hpy8I GTNNAC 2 cut(s) 195, 346
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 295, 393
HpyCH4V TGCA 2 cut(s) 143, 173
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
Hsp92II CATG 3 cut(s) 19, 241, 356
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 8 cut(s) 88, 116, 129, 143, 159, 168, 180, 293
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 186, 308
MaeI CTAG 3 cut(s) 59, 93, 375
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 3 cut(s) 113, 206, 216
MnlI CCTC 1 cut(s) 166
MslI CAYNNNNRTG 2 cut(s) 293, 351
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 1 cut(s) 97
NlaIII CATG 3 cut(s) 19, 241, 356
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 281
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 5 cut(s) 146, 195, 248, 279, 280
PstI CTGCAG 1 cut(s) 145
RsaI GTAC 2 cut(s) 125, 360
RsaNI GTAC 2 cut(s) 124, 359
RseI CAYNNNNRTG 2 cut(s) 293, 351
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 5 cut(s) 146, 195, 248, 279, 280
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 283
SetI ASST 8 cut(s) 27, 46, 88, 94, 148, 158, 192, 200
SfaNI GCATC 2 cut(s) 186, 308
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 3 cut(s) 146, 195, 248
SmiMI CAYNNNNRTG 2 cut(s) 293, 351
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 3 cut(s) 113, 206, 216
SspMI CTAG 3 cut(s) 59, 93, 375
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 295, 393
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 1 cut(s) 265
TasI AATT 3 cut(s) 113, 206, 216
TatI WGTACW 2 cut(s) 123, 358
TscAI CASTG 2 cut(s) 145, 312
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 4, 369
TspGWI ACGGA 2 cut(s) 256, 327
TspRI CASTG 2 cut(s) 145, 312
VpaK11BI GGWCC 3 cut(s) 146, 195, 248
XbaI TCTAGA 1 cut(s) 374
XspI CTAG 3 cut(s) 59, 93, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.