Rh2CG522200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0019882
Physical Location & Seq
Reverse (-)
69637139 .. 69637899
761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG522200.1

Sequence Viewer

Length: 390 bp
ATGGCATTCAACTTCATGTCGAAGCTCACTCAACAATCCCTACCACTACTACTAGTTTTGGTTTTTGCCAGAAGTTTAGCTGAAACTAGTGATCTCAAAGAAACACAATTAGTCTTGTACTTCCAGGACTTCACTGCAGGTCCAAATACCTCGAGCATACCAGTTGCAGGCATCGCCGGAAAGCTGTGGACCTTCACTCAATTCGGAACAATTTATGTCACCGATGACACCATGACCCAAGGACCGAACATAACATCTCCCCTAGTGGGACGGGCCCAAGGCATAACGGTGGCATCGGCACTGGACGGAAGCAATGCCCTTGTCTTGGTGTCTCGTGTTCACAAACATGAAGTACAATGGCAGCACTCTAGAGATACAAGGAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

129

Amino Acids

14.17

Weight (kDa)

8.12

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 35 - 111 2.8e-18 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019882)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0158841
rosa_multiflora Rmu_sc0014404.1_g000002
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538900 Rh2BG551500 Rh2CG522200 Rh2DG561300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AfaI GTAC 2 cut(s) 119, 354
AfiI CCNNNNNNNGG 3 cut(s) 167, 266, 325
AgsI TTSAA 1 cut(s) 10
AhlI ACTAGT 2 cut(s) 52, 86
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 3 cut(s) 25, 80, 184
AluI AGCT 3 cut(s) 25, 80, 184
Alw26I GTCTC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 1 cut(s) 273
ApaI GGGCCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 361
AspS9I GGNCC 5 cut(s) 140, 189, 242, 273, 274
AsuHPI GGTGA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 3 cut(s) 140, 189, 242
BaeGI GKGCMC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 277
BauI CACGAG 1 cut(s) 333
BbvI GCAGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 336
BcuI ACTAGT 2 cut(s) 52, 86
BfaI CTAG 4 cut(s) 53, 87, 263, 369
BfmI CTRYAG 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 1 cut(s) 362
BlsI GCNGC 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 3 cut(s) 140, 189, 242
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 5 cut(s) 140, 189, 242, 273, 274
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 180, 302
BsaJI CCNNGG 2 cut(s) 238, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 266, 325
Bse1I ACTGG 2 cut(s) 161, 306
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 238, 277
BseLI CCNNNNNNNGG 3 cut(s) 167, 266, 325
BseMI GCAATG 1 cut(s) 319
BseNI ACTGG 2 cut(s) 161, 306
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 1 cut(s) 373
BshFI GGCC 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 3 cut(s) 167, 266, 325
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 151
Bsp120I GGGCCC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 2 cut(s) 161, 306
BssECI CCNNGG 2 cut(s) 238, 277
BssMI GATC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 238, 277
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 289, 387
BstC8I GCNNGC 1 cut(s) 169
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 135
BstSLI GKGCMC 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 373
BsuRI GGCC 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 2 cut(s) 132, 299
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 5 cut(s) 140, 189, 242, 273, 274
Csp6I GTAC 2 cut(s) 118, 353
CviAII CATG 3 cut(s) 16, 232, 347
CviJI RGCY 4 cut(s) 25, 80, 184, 275
CviKI_1 RGCY 4 cut(s) 25, 80, 184, 275
CviQI GTAC 2 cut(s) 118, 353
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 238, 277
Eco24I GRGCYC 1 cut(s) 277
Eco47I GGWCC 3 cut(s) 140, 189, 242
Eco88I CYCGRG 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 2 cut(s) 238, 277
EcoT38I GRGCYC 1 cut(s) 277
ErhI CCWWGG 2 cut(s) 238, 277
FaeI CATG 3 cut(s) 19, 235, 350
FaiI YATR 7 cut(s) 17, 158, 216, 233, 251, 284, 348
FaqI GGGAC 1 cut(s) 282
FatI CATG 3 cut(s) 15, 231, 346
Fnu4HI GCNGC 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 1 cut(s) 362
FspBI CTAG 4 cut(s) 53, 87, 263, 369
GluI GCNGC 1 cut(s) 362
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 177
Hin1II CATG 3 cut(s) 19, 235, 350
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 189, 340
Hpy188I TCNGA 1 cut(s) 206
Hpy188III TCNNGA 1 cut(s) 369
Hpy8I GTNNAC 2 cut(s) 189, 340
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 289, 387
HpyCH4V TGCA 2 cut(s) 137, 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
Hsp92II CATG 3 cut(s) 19, 235, 350
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 8 cut(s) 82, 110, 123, 137, 153, 174, 190, 287
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 180, 302
MaeI CTAG 4 cut(s) 53, 87, 263, 369
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 3 cut(s) 107, 200, 210
MnlI CCTC 1 cut(s) 160
MslI CAYNNNNRTG 2 cut(s) 287, 345
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 1 cut(s) 91
NlaIII CATG 3 cut(s) 19, 235, 350
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 217
PaeR7I CTCGAG 1 cut(s) 151
PctI GAATGC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 275
PspOMI GGGCCC 1 cut(s) 273
PspPI GGNCC 5 cut(s) 140, 189, 242, 273, 274
PspXI VCTCGAGB 1 cut(s) 151
PstI CTGCAG 1 cut(s) 139
RsaI GTAC 2 cut(s) 119, 354
RsaNI GTAC 2 cut(s) 118, 353
RseI CAYNNNNRTG 2 cut(s) 287, 345
SatI GCNGC 1 cut(s) 362
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 5 cut(s) 140, 189, 242, 273, 274
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 277
SetI ASST 6 cut(s) 27, 82, 142, 152, 186, 194
SfaNI GCATC 2 cut(s) 180, 302
SfcI CTRYAG 1 cut(s) 135
Sfr274I CTCGAG 1 cut(s) 151
SinI GGWCC 3 cut(s) 140, 189, 242
SlaI CTCGAG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 287, 345
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SpeI ACTAGT 2 cut(s) 52, 86
Sse9I AATT 3 cut(s) 107, 200, 210
SspMI CTAG 4 cut(s) 53, 87, 263, 369
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 238, 277
TaaI ACNGT 2 cut(s) 289, 387
TaqI TCGA 2 cut(s) 20, 152
TaqII GACCGA 1 cut(s) 259
TasI AATT 3 cut(s) 107, 200, 210
TatI WGTACW 2 cut(s) 117, 352
TscAI CASTG 2 cut(s) 139, 306
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 361
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 4, 363
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 2 cut(s) 139, 306
VpaK11BI GGWCC 3 cut(s) 140, 189, 242
XbaI TCTAGA 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 151
XspI CTAG 4 cut(s) 53, 87, 263, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.