Rh2DG561300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0019882
Physical Location & Seq
Reverse (-)
79022872 .. 79023279
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG561300.1

Sequence Viewer

Length: 408 bp
ATGGCATTCAACTTCATGTCGAAGCTCACTCAACAATCCCTACCACTACTACTAGTTTTGGTTTTTGCCAGAAGTTTAGCTGAAGCTAGTGATCTCAAAGAAACCCAATTAGTCTTGTACTTCCAGGACTTCACAGCAGGTCCAAATACCTCGAGCATACCAGTTGCAGGCATCGCCGGAAAGCTGTGGACCTTCACTCAATTCGGAACAATCACTCAATTCGGAACAATTTATGTCACCGATGACACCATGACCCAAGGACCGAACATAACATCTTCCGTAGTGGGATGGGCCCAAGGCATAAGGGTGGCATTGGCACTGGACGGAAGCAATGCCCTTGTCTTGGTGTCTCGTGTTCACAAACATGAAGTACAATGGCAGCACTCTAGAGATACAAGGAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

135

Amino Acids

14.87

Weight (kDa)

8.12

Isoelectric Point (pI)

24.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 35 - 117 1.2e-14 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019882)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0158841
rosa_multiflora Rmu_sc0014404.1_g000002
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538900 Rh2BG551500 Rh2CG522200 Rh2DG561300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 2 cut(s) 119, 372
AfiI CCNNNNNNNGG 2 cut(s) 167, 343
AgsI TTSAA 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 4 cut(s) 25, 80, 86, 184
AluI AGCT 4 cut(s) 25, 80, 86, 184
Alw26I GTCTC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 151
AoxI GGCC 1 cut(s) 291
ApaI GGGCCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 379
AspS9I GGNCC 5 cut(s) 140, 189, 260, 291, 292
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 3 cut(s) 140, 189, 260
BaeGI GKGCMC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 295
BauI CACGAG 1 cut(s) 351
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 354
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 3 cut(s) 53, 87, 387
BfuAI ACCTGC 1 cut(s) 128
BisI GCNGC 1 cut(s) 380
BlsI GCNGC 1 cut(s) 381
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 3 cut(s) 140, 189, 260
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 5 cut(s) 140, 189, 260, 291, 292
BmiI GGNNCC 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 256, 295
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 343
Bse1I ACTGG 2 cut(s) 161, 324
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 256, 295
BseGI GGATG 1 cut(s) 293
BseLI CCNNNNNNNGG 2 cut(s) 167, 343
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 2 cut(s) 161, 324
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 1 cut(s) 391
BshFI GGCC 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 2 cut(s) 167, 343
BsmAI GTCTC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 1 cut(s) 293
BsoBI CYCGRG 1 cut(s) 151
Bsp120I GGGCCC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 1 cut(s) 293
BspLI GGNNCC 1 cut(s) 293
BspMI ACCTGC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 2 cut(s) 161, 324
BssECI CCNNGG 2 cut(s) 256, 295
BssMI GATC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 256, 295
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 169
BstF5I GGATG 1 cut(s) 293
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 295
BstV1I GCAGC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 293
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 5 cut(s) 140, 189, 260, 291, 292
Csp6I GTAC 2 cut(s) 118, 371
CviAII CATG 3 cut(s) 16, 250, 365
CviJI RGCY 5 cut(s) 25, 80, 86, 184, 293
CviKI_1 RGCY 5 cut(s) 25, 80, 86, 184, 293
CviQI GTAC 2 cut(s) 118, 371
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eco130I CCWWGG 2 cut(s) 256, 295
Eco24I GRGCYC 1 cut(s) 295
Eco47I GGWCC 3 cut(s) 140, 189, 260
Eco57I CTGAAG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 2 cut(s) 256, 295
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 2 cut(s) 256, 295
FaeI CATG 3 cut(s) 19, 253, 368
FaiI YATR 7 cut(s) 17, 158, 234, 251, 269, 302, 366
FatI CATG 3 cut(s) 15, 249, 364
Fnu4HI GCNGC 1 cut(s) 380
FokI GGATG 1 cut(s) 300
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 380
FspBI CTAG 3 cut(s) 53, 87, 387
GluI GCNGC 1 cut(s) 380
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 177
Hin1II CATG 3 cut(s) 19, 253, 368
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 189, 358
Hpy188I TCNGA 2 cut(s) 206, 224
Hpy188III TCNNGA 1 cut(s) 387
Hpy8I GTNNAC 2 cut(s) 189, 358
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4V TGCA 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
Hsp92II CATG 3 cut(s) 19, 253, 368
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 8 cut(s) 82, 110, 123, 137, 153, 174, 190, 305
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 180
MaeI CTAG 3 cut(s) 53, 87, 387
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 4 cut(s) 107, 200, 218, 228
MnlI CCTC 1 cut(s) 160
MslI CAYNNNNRTG 2 cut(s) 305, 363
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 1 cut(s) 91
NlaIII CATG 3 cut(s) 19, 253, 368
NlaIV GGNNCC 1 cut(s) 293
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 151
PctI GAATGC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 293
PspOMI GGGCCC 1 cut(s) 291
PspPI GGNCC 5 cut(s) 140, 189, 260, 291, 292
PspXI VCTCGAGB 1 cut(s) 151
RsaI GTAC 2 cut(s) 119, 372
RsaNI GTAC 2 cut(s) 118, 371
RseI CAYNNNNRTG 2 cut(s) 305, 363
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 5 cut(s) 140, 189, 260, 291, 292
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 295
SetI ASST 7 cut(s) 27, 82, 88, 142, 152, 186, 194
SfaNI GCATC 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 151
SinI GGWCC 3 cut(s) 140, 189, 260
SlaI CTCGAG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 305, 363
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 4 cut(s) 107, 200, 218, 228
SspMI CTAG 3 cut(s) 53, 87, 387
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 256, 295
TaaI ACNGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 20, 152
TaqII GACCGA 1 cut(s) 277
TasI AATT 4 cut(s) 107, 200, 218, 228
TatI WGTACW 2 cut(s) 117, 370
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 4, 381
TspGWI ACGGA 2 cut(s) 268, 339
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 3 cut(s) 140, 189, 260
XbaI TCTAGA 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 151
XspI CTAG 3 cut(s) 53, 87, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.