Rorug02G0473000

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000369
Physical Location & Seq
Forward (+)
60451667 .. 60460007
8341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0473000.1

Sequence Viewer

Length: 750 bp
ATGGAGTTCCCAAAACAAATTACACCAGCTGATGATCCTGAGAGCTCTTCCCAAAAGAAATTGGGAAGAGGGAAAATCGAGATCAAGAGGATCGAAAACACTACCAATCGACAAGTCACATTCTGCAAGCGCCGCAACGGTTTGCTTAAGAAAGCATATGAACTATCTGTTCTTTGTGATGCTGAAGTTGCTCTTATTGTTTTCTCTACCCGGGGGCGCCTCTATGAATATGCTAACAACAGTGTTAGAGCAACAATCGAAAGGTACAAGAAAGCATGTGATTCTTCAAACACTGGATCTGTAACTGAGACTAATGTTCAGTTTTATCAGCAAGAAGCCTCCAAACTAAGAAGACAGATAAGAGAAATTCAGAATTCAAACAGGCACATACTAGGTGAAGCTCTTAGCACTTTGAACGTCAAGGAACTCAAGAACCTAGAAGGAAGATTGGAGAAAGGAATCAGCAGAATAAGATCCAAAAAGAATGAAATGCTGTTTGCTGAAATCGAATATATGCAAAAGAGGGAGATCGAGCTGCAAAATCATAACAATTTTCTGAGAGCAAAGATTGCTGAAAATGACAGGGCACAACAGCAGCAAGCAAATATGATGCCAGGAACATCATCTGCGTATGATCAATCAATGCCTCCTCCTCAATCATACGATAGGAGCTTTCTCCCTGTAATCCTTGAGTCCAATCACCACTACAATCGCCAAGGGCAGAACCAGACACCTCTCCAACTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

249

Amino Acids

28.77

Weight (kDa)

9.51

Isoelectric Point (pI)

70.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 30 - 77 2.1e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 103 - 190 2.3e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000369)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12960 FvH4_1g13711 FvH4_1g13821 FvH4_1g13851 FvH4_2g14150 FvH4_2g21221 FvH4_2g29121 FvH4_5g08370 FvH4_6g01270 FvH4_6g01284 FvH4_6g17580
malus_domestica MD14G1060300.v1.1 MD17G1230300.v1.1
prunus_persica Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.6G256100_v2.0.a1
pyrus_communis pycom17g23320
rosa_chinensis RchiOBHm_Chr1g0317281 RchiOBHm_Chr2g0158871 RchiOBHm_Chr3g0471851 RchiOBHm_Chr6g0287731 RchiOBHm_Chr6g0287821 RchiOBHm_Chr7g0183811
rosa_laevigata RLG00000005018 RLG00000005021 RLG00000012470 RLG00000017037 RLG00000021119 RLG00000024124 RLG00000029577 RLG00000030613 RLG00000030616
rosa_multiflora Rmu_sc0000423.1_g000029 Rmu_sc0002267.1_g000011 Rmu_sc0003335.1_g000004 Rmu_sc0003610.1_g000042 Rmu_sc0003877.1_g000013 Rmu_sc0005698.1_g000001 Rmu_sc0007593.1_g000004 Rmu_sc0013966.1_g000003 Rmu_sc0015519.1_g000001
rosa_roxburghii Rroxscaffold_2G00141640 Rroxscaffold_3G00270160 Rroxscaffold_3G00270190 Rroxscaffold_4G00327520 Rroxscaffold_6G00409480 Rroxscaffold_7G00180790 Rroxscaffold_7G00181110 Rroxscaffold_7G00181180 Rroxscaffold_7G00181240
rosa_rugosa Rorug01G0015500 Rorug02G0095300 Rorug02G0473000 Rorug03G0120700 Rorug06G0191300 Rorug06G0191500 Rorug06G0460600 Rorug06G0461000
rosa_samantha Rh1AG026600 Rh1BG022800 Rh1DG047100 Rh1DG054100 Rh2AG142300 Rh2AG142400 Rh2BG147400 Rh2BG147500 Rh2BG551700 Rh2CG148700 Rh2CG148800 Rh2CG522400 Rh2DG148000 Rh2DG148100 Rh2DG561500 Rh3AG172000 Rh3BG197400 Rh3CG188400 Rh3CG196000 Rh3DG176900 Rh6AG301500 Rh6AG301800 Rh6BG307300 Rh6BG307800 Rh6DG299000 Rh6DG299200 Rh6DG300300 Rh6DG300400 Rh7AG061300 Rh7AG061700 Rh7BG061300 Rh7BG061400 Rh7BG061800 Rh7CG063000 Rh7CG063200 Rh7CG063300 Rh7DG061400
rosa_wichuraiana Rw1G001850 Rw2G011170 Rw2G044620 Rw3G016850 Rw6G026030 Rw6G026070 Rw7G005090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 216
AciI CCGC 1 cut(s) 133
AclWI GGATC 4 cut(s) 29, 98, 304, 468
AcsI RAATTY 2 cut(s) 366, 373
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 1 cut(s) 266
AflII CTTAAG 1 cut(s) 146
AgsI TTSAA 3 cut(s) 288, 378, 415
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 5 cut(s) 29, 45, 401, 535, 672
AluI AGCT 5 cut(s) 29, 45, 401, 535, 672
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 4 cut(s) 29, 98, 304, 468
Ama87I CYCGRG 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 535, 595
ApoI RAATTY 2 cut(s) 366, 373
AspLEI GCGC 2 cut(s) 132, 219
AsuC2I CCSGG 2 cut(s) 211, 212
AsuHPI GGTGA 2 cut(s) 407, 692
AvaI CYCGRG 1 cut(s) 210
BaeGI GKGCMC 1 cut(s) 589
BanI GGYRCC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 2 cut(s) 522, 607
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 2 cut(s) 211, 212
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 2 cut(s) 392, 437
BfoI RGCGCY 2 cut(s) 133, 220
BfrI CTTAAG 1 cut(s) 146
BisI GCNGC 3 cut(s) 133, 536, 596
BlsI GCNGC 3 cut(s) 134, 537, 597
Bme1390I CCNGG 3 cut(s) 211, 212, 615
BmeT110I CYCGRG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 3 cut(s) 211, 212, 615
BmsI GCATC 2 cut(s) 169, 600
BpiI GAAGAC 1 cut(s) 358
BpuEI CTTGAG 2 cut(s) 413, 710
BpuMI CCSGG 2 cut(s) 211, 212
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 210, 211, 715
Bse1I ACTGG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 210, 211, 715
BseMII CTCAG 3 cut(s) 30, 297, 548
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 2 cut(s) 639, 642
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 2 cut(s) 522, 607
BshNI GGYRCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 47, 589
Bsp143I GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
BspACI CCGC 1 cut(s) 133
BspCNI CTCAG 3 cut(s) 31, 298, 549
BspLI GGNNCC 1 cut(s) 218
BspPI GGATC 4 cut(s) 29, 98, 304, 468
BspQI GCTCTTC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 216
BspTI CTTAAG 1 cut(s) 146
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 3 cut(s) 210, 211, 715
BssMI GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
BssNI GRCGYC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 715
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 140, 242
Bst6I CTCTTC 2 cut(s) 52, 61
BstACI GRCGYC 1 cut(s) 217
BstAFI CTTAAG 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 128, 600
BstDEI CTNAG 5 cut(s) 39, 306, 347, 404, 557
BstH2I RGCGCY 2 cut(s) 133, 220
BstHHI GCGC 2 cut(s) 132, 219
BstKTI GATC 7 cut(s) 37, 84, 93, 299, 476, 531, 637
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
BstMWI GCNNNNNNNGC 3 cut(s) 132, 188, 569
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 3 cut(s) 209, 210, 613
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 2 cut(s) 522, 607
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 2 cut(s) 296, 473
BstYI RGATCY 2 cut(s) 296, 473
BtsIMutI CAGTG 2 cut(s) 247, 291
Cac8I GCNNGC 2 cut(s) 128, 600
CfoI GCGC 2 cut(s) 132, 219
Cfr9I CCCGGG 1 cut(s) 210
Csp6I GTAC 1 cut(s) 265
CviAII CATG 1 cut(s) 276
CviJI RGCY 6 cut(s) 29, 45, 338, 401, 535, 672
CviKI_1 RGCY 6 cut(s) 29, 45, 338, 401, 535, 672
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 5 cut(s) 39, 306, 347, 404, 557
DinI GGCGCC 1 cut(s) 218
DpnI GATC 7 cut(s) 36, 83, 92, 298, 475, 530, 636
DpnII GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
Eam1104I CTCTTC 2 cut(s) 52, 61
EarI CTCTTC 2 cut(s) 52, 61
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 218
EheI GGCGCC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 715
FaeI CATG 1 cut(s) 279
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FatI CATG 1 cut(s) 275
FauNDI CATATG 1 cut(s) 157
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 3 cut(s) 133, 536, 596
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 133, 536, 596
FspBI CTAG 2 cut(s) 392, 437
GlaI GCGC 2 cut(s) 131, 218
GluI GCNGC 3 cut(s) 133, 536, 596
HaeII RGCGCY 2 cut(s) 133, 220
HapII CCGG 1 cut(s) 211
HhaI GCGC 2 cut(s) 132, 219
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 1 cut(s) 279
Hin6I GCGC 2 cut(s) 130, 217
HinP1I GCGC 2 cut(s) 130, 217
HinfI GANTC 3 cut(s) 281, 459, 692
HpaII CCGG 1 cut(s) 211
HphI GGTGA 2 cut(s) 407, 692
Hpy188I TCNGA 2 cut(s) 372, 558
Hpy188III TCNNGA 4 cut(s) 38, 79, 85, 430
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 2 cut(s) 140, 242
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 3 cut(s) 126, 517, 538
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 188, 569
HpyF3I CTNAG 5 cut(s) 39, 306, 347, 404, 557
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 1 cut(s) 279
HspAI GCGC 2 cut(s) 130, 217
KasI GGCGCC 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 669
Lsp1109I GCAGC 2 cut(s) 522, 607
LweI GCATC 2 cut(s) 169, 600
MaeI CTAG 2 cut(s) 392, 437
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 115, 301
MalI GATC 7 cut(s) 36, 83, 92, 298, 475, 530, 636
MboI GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
MboII GAAGA 5 cut(s) 39, 78, 276, 363, 456
MflI RGATCY 2 cut(s) 296, 473
MhlI GDGCHC 2 cut(s) 47, 589
MluCI AATT 5 cut(s) 18, 59, 366, 373, 550
Mly113I GGCGCC 1 cut(s) 217
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 9 cut(s) 62, 81, 230, 349, 516, 657, 660, 663, 744
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 146
MspI CCGG 1 cut(s) 211
MspR9I CCNGG 3 cut(s) 211, 212, 615
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 3 cut(s) 132, 188, 569
NarI GGCGCC 1 cut(s) 217
NciI CCSGG 2 cut(s) 211, 212
NdeI CATATG 1 cut(s) 157
NdeII GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
NlaIII CATG 1 cut(s) 279
NlaIV GGNNCC 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 52
PfeI GAWTC 2 cut(s) 281, 459
PkrI GCNGC 3 cut(s) 134, 537, 597
PleI GAGTC 1 cut(s) 700
PluTI GGCGCC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 700
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 1 cut(s) 218
PsuI RGATCY 2 cut(s) 296, 473
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SacI GAGCTC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 3 cut(s) 133, 536, 596
Sau3AI GATC 7 cut(s) 34, 81, 90, 296, 473, 528, 634
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 3 cut(s) 211, 212, 615
SduI GDGCHC 2 cut(s) 47, 589
SfaNI GCATC 2 cut(s) 169, 600
SfoI GGCGCC 1 cut(s) 218
SmaI CCCGGG 1 cut(s) 212
SmlI CTYRAG 3 cut(s) 146, 428, 689
SmoI CTYRAG 3 cut(s) 146, 428, 689
Sse9I AATT 5 cut(s) 18, 59, 366, 373, 550
SsiI CCGC 1 cut(s) 133
SspDI GGCGCC 1 cut(s) 216
SspMI CTAG 2 cut(s) 392, 437
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 209, 210, 613
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 2 cut(s) 140, 242
TaiI ACGT 1 cut(s) 420
TaqI TCGA 6 cut(s) 78, 93, 109, 258, 507, 531
TasI AATT 5 cut(s) 18, 59, 366, 373, 550
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 2 cut(s) 281, 459
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 2 cut(s) 247, 298
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 535, 595
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 3 cut(s) 174, 240, 501
TspMI CCCGGG 1 cut(s) 210
TspRI CASTG 2 cut(s) 247, 298
Vha464I CTTAAG 1 cut(s) 146
XapI RAATTY 2 cut(s) 366, 373
XceI RCATGY 1 cut(s) 279
XmaI CCCGGG 1 cut(s) 210
XspI CTAG 2 cut(s) 392, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.