Rh2CG522400

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000369
Physical Location & Seq
Forward (+)
69668934 .. 69670268
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG522400.1

Sequence Viewer

Length: 1335 bp
ATGGAAACTAGTAGAAGCCAAGTTGTAAAGCTGAGCTATACTTTGGCTCTAGGAATACCTGAAAATGTCCTAGTTGCAATTCTCTCAAAACTGCCTGTCAAGTCTTTACTTAGGTTTCTCTGCGTCTCGCAAGAGTGGCATGCCCTCACCAAAAGTTCTTACTTCATTGCCAGACACCTTGTTCATCAATATTCTAGCTGCTCCGATCAGAGTCGTCCTTGTCTTCTCTTTCTCTCGAGCCTAGCTGGAAGCTTTGAAGATGATTGCAAGCCACGACTCGGATTCTCGTTGCTTCGTGATGAAGAAATGACGTTTTTAGGATTACCTTCCCTGCCGAACCTTACAATCAAAACAGCGGCCCTAAAGCTCGTGGGATCTAGTAATGGCCTAGTTTTTTGTCATGTCATTGACACTGAGAATTGGACTAGTACTACAGAAGAGAAGCATGATATTGCTTCTAGATTTAGAGAATTATTTTTAGTATGGAACCCAGCAACACAACAATTTAAGTGTCTTCCTGAACCTAAAAATAATATTTCTTTGGAGCAAGAGGAGGAGGCTACTTCTGTTCTTTTCGCTTTTGATTGTGTACATGATCATGACATTAGTGAATACAAATTGGTGAGAGTTTTTTCTCATGGGAGTGATGATCCCACAGATCATGGGAAAGAGGTTACTACTTTTGAAGCTCAAGTTTTCATCCGAAGTACAAATTCTTGGAGGCAAGTCAAGGACAAATTAGCGTTTCCATCATATAAACGTTGTTTTGAATCAACTGCAACTACATTGAATGGAGTGCTGTATTGGAAGGTAGTGCAAAAATCCGATGAGTGCTACGTTCTGTCATTCAGTTTACATGAAGAGGTTTTCAGCATTATACAGCTACCAATATGTATACGTTACCTTGATCGTGACGATTATAACTGGACCACAGTACCTTTATATTCATGGAAAGATTCACCTGCAATTGTAGTAACCGATTATTCAGCCAATGGCAATCCCAACATAGTGTTTTGGGTGATGAGTACCACTGAAGAATCAAAGAGTAATACTAATGAGAGTGGTACACAAATGCGAACACCAACTTGGGTTCAACAAGTAAGACTCAAATACTCAGTACTAGATGGATTTAGCATGATAGGACTTTGGAAGGATCAATTTCTTTTCACATCCAAGAAAACAAATGAAGCTGCTGGTGCCACAGATGATATTGAGCCCGAAGACCTGTTTCTGTACGACCCCAGAAGTGATACCAAGAGGGAGATTGTGAAACATGCAGTAAGGATCAAGTTTATACGAGTAGAAAATTATGTGGAGAGCCTACTTCCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

444

Amino Acids

50.81

Weight (kDa)

5.56

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 18 - 55 8.6e-07 F-box domain
F-box-like PF12937 19 - 55 3.6e-06 F-box-like
FBA_3 PF08268 149 - 348 1.1e-16 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000369)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12960 FvH4_1g13711 FvH4_1g13821 FvH4_1g13851 FvH4_2g14150 FvH4_2g21221 FvH4_2g29121 FvH4_5g08370 FvH4_6g01270 FvH4_6g01284 FvH4_6g17580
malus_domestica MD14G1060300.v1.1 MD17G1230300.v1.1
prunus_persica Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.3G110000_v2.0.a1 Prupe.6G256100_v2.0.a1
pyrus_communis pycom17g23320
rosa_chinensis RchiOBHm_Chr1g0317281 RchiOBHm_Chr2g0158871 RchiOBHm_Chr3g0471851 RchiOBHm_Chr6g0287731 RchiOBHm_Chr6g0287821 RchiOBHm_Chr7g0183811
rosa_laevigata RLG00000005018 RLG00000005021 RLG00000012470 RLG00000017037 RLG00000021119 RLG00000024124 RLG00000029577 RLG00000030613 RLG00000030616
rosa_multiflora Rmu_sc0000423.1_g000029 Rmu_sc0002267.1_g000011 Rmu_sc0003335.1_g000004 Rmu_sc0003610.1_g000042 Rmu_sc0003877.1_g000013 Rmu_sc0005698.1_g000001 Rmu_sc0007593.1_g000004 Rmu_sc0013966.1_g000003 Rmu_sc0015519.1_g000001
rosa_roxburghii Rroxscaffold_2G00141640 Rroxscaffold_3G00270160 Rroxscaffold_3G00270190 Rroxscaffold_4G00327520 Rroxscaffold_6G00409480 Rroxscaffold_7G00180790 Rroxscaffold_7G00181110 Rroxscaffold_7G00181180 Rroxscaffold_7G00181240
rosa_rugosa Rorug01G0015500 Rorug02G0095300 Rorug02G0473000 Rorug03G0120700 Rorug06G0191300 Rorug06G0191500 Rorug06G0460600 Rorug06G0461000
rosa_samantha Rh1AG026600 Rh1BG022800 Rh1DG047100 Rh1DG054100 Rh2AG142300 Rh2AG142400 Rh2BG147400 Rh2BG147500 Rh2BG551700 Rh2CG148700 Rh2CG148800 Rh2CG522400 Rh2DG148000 Rh2DG148100 Rh2DG561500 Rh3AG172000 Rh3BG197400 Rh3CG188400 Rh3CG196000 Rh3DG176900 Rh6AG301500 Rh6AG301800 Rh6BG307300 Rh6BG307800 Rh6DG299000 Rh6DG299200 Rh6DG300300 Rh6DG300400 Rh7AG061300 Rh7AG061700 Rh7BG061300 Rh7BG061400 Rh7BG061800 Rh7CG063000 Rh7CG063200 Rh7CG063300 Rh7DG061400
rosa_wichuraiana Rw1G001850 Rw2G011170 Rw2G044620 Rw3G016850 Rw6G026030 Rw6G026070 Rw7G005090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 1196
AccI GTMKAC 1 cut(s) 895
AciI CCGC 1 cut(s) 356
AclI AACGTT 1 cut(s) 760
AclWI GGATC 4 cut(s) 382, 644, 1161, 1292
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 1053
AfaI GTAC 8 cut(s) 430, 591, 709, 936, 1027, 1066, 1119, 1235
AfiI CCNNNNNNNGG 1 cut(s) 278
AgsI TTSAA 5 cut(s) 257, 686, 770, 790, 1094
AhlI ACTAGT 2 cut(s) 8, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 1069, 1101
AluBI AGCT 9 cut(s) 31, 36, 198, 245, 252, 367, 689, 883, 1190
AluI AGCT 9 cut(s) 31, 36, 198, 245, 252, 367, 689, 883, 1190
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 4 cut(s) 382, 644, 1161, 1292
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 2 cut(s) 357, 385
ApeKI GCWGC 2 cut(s) 198, 1190
ApoI RAATTY 1 cut(s) 712
AspS9I GGNCC 2 cut(s) 358, 927
AsuHPI GGTGA 4 cut(s) 139, 634, 951, 1030
AvaI CYCGRG 1 cut(s) 235
AvaII GGWCC 1 cut(s) 927
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 1218
BauI CACGAG 1 cut(s) 368
BbsI GAAGAC 3 cut(s) 215, 506, 1227
BbvI GCAGC 2 cut(s) 185, 1177
BccI CCATC 2 cut(s) 757, 1118
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 1 cut(s) 130
BcuI ACTAGT 2 cut(s) 8, 425
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 3 cut(s) 199, 357, 1191
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 3 cut(s) 200, 358, 1192
BmcAI AGTACT 2 cut(s) 430, 1119
Bme18I GGWCC 1 cut(s) 927
BmeT110I CYCGRG 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 358, 927
BmiI GGNNCC 2 cut(s) 488, 1198
BpiI GAAGAC 3 cut(s) 215, 506, 1227
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 675
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 929, 1328
Bse3DI GCAATG 1 cut(s) 165
BseGI GGATG 2 cut(s) 699, 1169
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 3 cut(s) 23, 405, 1128
BseNI ACTGG 2 cut(s) 929, 1328
BseRI GAGGAG 2 cut(s) 566, 569
BseXI GCAGC 2 cut(s) 185, 1177
BseYI CCCAGC 1 cut(s) 490
BshFI GGCC 2 cut(s) 359, 387
BshNI GGYRCC 1 cut(s) 1196
BsiHKCI CYCGRG 1 cut(s) 235
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 1 cut(s) 130
BsmBI CGTCTC 1 cut(s) 130
BsnI GGCC 2 cut(s) 359, 387
BsoBI CYCGRG 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 1218
Bsp1407I TGTACA 1 cut(s) 589
Bsp143I GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 1 cut(s) 356
BspANI GGCC 2 cut(s) 359, 387
BspCNI CTCAG 3 cut(s) 24, 406, 1127
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 488, 1198
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 4 cut(s) 382, 644, 1161, 1292
BspT107I GGYRCC 1 cut(s) 1196
BsrDI GCAATG 1 cut(s) 165
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 2 cut(s) 929, 1328
BssMI GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
BssNAI GTATAC 1 cut(s) 896
BssSI CACGAG 1 cut(s) 368
Bst1107I GTATAC 1 cut(s) 896
Bst2BI CACGAG 1 cut(s) 368
Bst4CI ACNGT 1 cut(s) 934
Bst6I CTCTTC 2 cut(s) 432, 855
BstAUI TGTACA 1 cut(s) 589
BstC8I GCNNGC 2 cut(s) 141, 269
BstDEI CTNAG 4 cut(s) 32, 110, 414, 1114
BstF5I GGATG 2 cut(s) 699, 1169
BstKTI GATC 8 cut(s) 208, 377, 598, 652, 661, 910, 1156, 1287
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
BstMWI GCNNNNNNNGC 2 cut(s) 136, 1196
BstNSI RCATGY 2 cut(s) 143, 1277
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 2 cut(s) 185, 1177
BstV2I GAAGAC 3 cut(s) 215, 506, 1227
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BstZ17I GTATAC 1 cut(s) 896
BsuRI GGCC 2 cut(s) 359, 387
BtsCI GGATG 2 cut(s) 699, 1169
BtsIMutI CAGTG 2 cut(s) 411, 1029
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 2 cut(s) 141, 269
CciI TCATGA 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 358, 927
CseI GACGC 1 cut(s) 112
Csp6I GTAC 8 cut(s) 429, 590, 708, 935, 1026, 1065, 1118, 1234
CviQI GTAC 8 cut(s) 429, 590, 708, 935, 1026, 1065, 1118, 1234
DdeI CTNAG 4 cut(s) 32, 110, 414, 1114
DpnI GATC 8 cut(s) 207, 376, 597, 651, 660, 909, 1155, 1286
DpnII GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
Eam1104I CTCTTC 2 cut(s) 432, 855
EarI CTCTTC 2 cut(s) 432, 855
Eco24I GRGCYC 1 cut(s) 1218
Eco47I GGWCC 1 cut(s) 927
Eco57I CTGAAG 1 cut(s) 1053
Eco88I CYCGRG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 1218
Esp3I CGTCTC 1 cut(s) 130
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 895
Fnu4HI GCNGC 3 cut(s) 199, 357, 1191
FokI GGATG 2 cut(s) 686, 1156
FriOI GRGCYC 1 cut(s) 1218
Fsp4HI GCNGC 3 cut(s) 199, 357, 1191
GluI GCNGC 3 cut(s) 199, 357, 1191
GsaI CCCAGC 1 cut(s) 494
HaeIII GGCC 2 cut(s) 359, 387
HgaI GACGC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 250
HinfI GANTC 7 cut(s) 211, 276, 282, 770, 956, 1037, 1104
HphI GGTGA 4 cut(s) 139, 634, 951, 1030
Hpy166II GTNNAC 4 cut(s) 590, 854, 896, 1067
Hpy188I TCNGA 5 cut(s) 205, 210, 281, 704, 826
Hpy188III TCNNGA 6 cut(s) 235, 296, 459, 518, 599, 911
Hpy8I GTNNAC 4 cut(s) 590, 854, 896, 1067
HpyAV CCTTC 3 cut(s) 336, 802, 1144
HpyCH4III ACNGT 1 cut(s) 934
HpyCH4IV ACGT 4 cut(s) 311, 760, 837, 898
HpyCH4V TGCA 6 cut(s) 77, 267, 779, 817, 965, 1277
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 1196
HpyF3I CTNAG 4 cut(s) 32, 110, 414, 1114
HpySE526I ACGT 4 cut(s) 311, 760, 837, 898
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
LmnI GCTCC 2 cut(s) 206, 544
Lsp1109I GCAGC 2 cut(s) 185, 1177
MaeII ACGT 4 cut(s) 311, 760, 837, 898
MaeIII GTNAC 4 cut(s) 673, 899, 911, 973
MalI GATC 8 cut(s) 207, 376, 597, 651, 660, 909, 1155, 1286
MboI GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
MboII GAAGA 8 cut(s) 215, 269, 314, 449, 506, 872, 1046, 1232
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 1218
MlyI GAGTC 3 cut(s) 220, 270, 1098
MnlI CCTC 8 cut(s) 155, 544, 547, 550, 664, 714, 856, 1251
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 2 cut(s) 597, 642
MspA1I CMGCKG 1 cut(s) 356
MunI CAATTG 1 cut(s) 966
MwoI GCNNNNNNNGC 2 cut(s) 136, 1196
NdeII GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
NlaIV GGNNCC 2 cut(s) 488, 1198
NmuCI GTSAC 1 cut(s) 911
NspI RCATGY 2 cut(s) 143, 1277
PaeI GCATGC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 235
PagI TCATGA 1 cut(s) 598
PaqCI CACCTGC 1 cut(s) 970
PfeI GAWTC 4 cut(s) 282, 770, 956, 1037
PkrI GCNGC 3 cut(s) 200, 358, 1192
PleI GAGTC 3 cut(s) 219, 270, 1098
PpsI GAGTC 3 cut(s) 219, 270, 1098
PsiI TTATAA 1 cut(s) 921
Psp1406I AACGTT 1 cut(s) 760
PspFI CCCAGC 1 cut(s) 490
PspN4I GGNNCC 2 cut(s) 488, 1198
PspPI GGNCC 2 cut(s) 358, 927
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 8 cut(s) 430, 591, 709, 936, 1027, 1066, 1119, 1235
RsaNI GTAC 8 cut(s) 429, 590, 708, 935, 1026, 1065, 1118, 1234
RseI CAYNNNNRTG 2 cut(s) 597, 642
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 3 cut(s) 199, 357, 1191
Sau3AI GATC 8 cut(s) 205, 374, 595, 649, 658, 907, 1153, 1284
Sau96I GGNCC 2 cut(s) 358, 927
ScaI AGTACT 2 cut(s) 430, 1119
SchI GAGTC 3 cut(s) 220, 270, 1098
SduI GDGCHC 1 cut(s) 1218
SfcI CTRYAG 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 927
SlaI CTCGAG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 597, 642
SmlI CTYRAG 2 cut(s) 235, 690
SmoI CTYRAG 2 cut(s) 235, 690
SpeI ACTAGT 2 cut(s) 8, 425
SphI GCATGC 1 cut(s) 143
SsiI CCGC 1 cut(s) 356
SspI AATATT 2 cut(s) 191, 535
TaaI ACNGT 1 cut(s) 934
TaiI ACGT 4 cut(s) 314, 763, 840, 901
TaqI TCGA 1 cut(s) 236
TatI WGTACW 4 cut(s) 428, 589, 707, 1117
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 4 cut(s) 282, 770, 956, 1037
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 2 cut(s) 418, 1036
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 198, 1190
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 7 cut(s) 154, 173, 315, 688, 873, 936, 1200
TspRI CASTG 2 cut(s) 418, 1036
VpaK11BI GGWCC 1 cut(s) 927
XapI RAATTY 1 cut(s) 712
XbaI TCTAGA 1 cut(s) 458
XceI RCATGY 2 cut(s) 143, 1277
XhoI CTCGAG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 895
ZrmI AGTACT 2 cut(s) 430, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.