RchiOBHm_Chr2g0165331

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019880
Physical Location & Seq
Reverse (-)
80343549 .. 80344978
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53329

Sequence Viewer

Length: 573 bp
ATGCTCAAGGGCCTCCTCCTTGAGCCGAGTCTATGTGATCTTGAGTGCATCTTCACTGTCATCTGCAATGTTGTTACAAATTCTGATTCTCTAGATGAAGTATGTCAAATGGCAGAACTTATATCTGGAAAGGTTACACAGCAACCAAATGACAAACCTGCATCGCGCTTAAAGATTCGGTTCTCTGTTTACTTGAGCGCTCTAAATTTGGCCATCAATGGAAAGGTCACTGAGCATGTCCTCCCTTCATTCAAACAGATAGATAGTTTCTTGAAGGGGTGGAATATTGGGGTCTTGGACCAGAGGCAGCTATTTCTTACCATATCTAATGTTTTAAAAGAACGTAAAAGCTTGGCAAAGGAGTCTTTCAAGTTCCTAACTAAGTACATGGCAACATTATCTGGCGAAGATGCACCTGTTTTGGGCCAAGCCAAGGATGAGGCTGTTTGCACAATTGTGGAATTTGTGAAGACACCAGACATGTTTCAGCTTATTGTAATGTCTTGTGGAATTTCAAAGATGTTGCATAGCACTTTCGTAGTCTTATTTCTACTTTGTGCGTGTTCATCCTAG

Protein Analysis

190

Amino Acids

21.05

Weight (kDa)

6.71

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019880)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0165331 RchiOBHm_Chr2g0165451
rosa_laevigata RLG00000021526
rosa_multiflora Rmu_sc0000655.1_g000019
rosa_samantha Rh2BG594100
rosa_wichuraiana Rw2G048440 Rw2G048490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 166
AccII CGCG 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 4 cut(s) 79, 205, 461, 510
AfaI GTAC 1 cut(s) 386
AfeI AGCGCT 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 422, 433
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 253, 274, 370, 516
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 3 cut(s) 310, 351, 490
AluI AGCT 3 cut(s) 310, 351, 490
Aor51HI AGCGCT 1 cut(s) 199
AoxI GGCC 3 cut(s) 10, 210, 424
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 4 cut(s) 79, 205, 461, 510
AspLEI GCGC 2 cut(s) 168, 200
AspS9I GGNCC 3 cut(s) 10, 298, 424
AvaII GGWCC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 476
BbvI GCAGC 1 cut(s) 319
BccI CCATC 1 cut(s) 221
BfaI CTAG 2 cut(s) 92, 571
BfoI RGCGCY 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 10, 298, 424
BmsI GCATC 3 cut(s) 57, 170, 400
BpiI GAAGAC 1 cut(s) 476
BpuEI CTTGAG 3 cut(s) 41, 62, 214
BsaJI CCNNGG 1 cut(s) 432
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 433
Bse3DI GCAATG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 2 cut(s) 442, 566
BseLI CCNNNNNNNGG 2 cut(s) 422, 433
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 5
BseXI GCAGC 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 3 cut(s) 12, 212, 426
BslI CCNNNNNNNGG 2 cut(s) 422, 433
BsnI GGCC 3 cut(s) 12, 212, 426
Bsp143I GATC 1 cut(s) 37
BspANI GGCC 3 cut(s) 12, 212, 426
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 231, 381
BstF5I GGATG 2 cut(s) 442, 566
BstFNI CGCG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 168, 200
BstKTI GATC 1 cut(s) 40
BstMBI GATC 1 cut(s) 37
BstNSI RCATGY 2 cut(s) 239, 484
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 3 cut(s) 12, 212, 426
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 442, 566
BtsIMutI CAGTG 2 cut(s) 54, 228
BveI ACCTGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 168, 200
Cfr13I GGNCC 3 cut(s) 10, 298, 424
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 236, 388, 481
CviJI RGCY 9 cut(s) 12, 25, 212, 310, 351, 426, 431, 443, 490
CviKI_1 RGCY 9 cut(s) 12, 25, 212, 310, 351, 426, 431, 443, 490
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 231, 381
DpnI GATC 1 cut(s) 39
DpnII GATC 1 cut(s) 37
DraI TTTAAA 1 cut(s) 336
EaeI YGGCCR 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 298
Eco47III AGCGCT 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 239, 391, 484
FaiI YATR 8 cut(s) 34, 103, 122, 237, 323, 389, 482, 528
FatI CATG 3 cut(s) 235, 387, 480
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 2 cut(s) 449, 553
Fsp4HI GCNGC 1 cut(s) 308
FspBI CTAG 2 cut(s) 92, 571
GlaI GCGC 2 cut(s) 167, 199
GluI GCNGC 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 3 cut(s) 12, 212, 426
HhaI GCGC 2 cut(s) 168, 200
Hin1II CATG 3 cut(s) 239, 391, 484
Hin6I GCGC 2 cut(s) 166, 198
HinP1I GCGC 2 cut(s) 166, 198
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 4 cut(s) 28, 86, 175, 362
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 4 cut(s) 41, 92, 126, 271
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 2 cut(s) 255, 268
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 6 cut(s) 48, 66, 161, 413, 450, 526
HpyF3I CTNAG 2 cut(s) 231, 381
HpySE526I ACGT 1 cut(s) 343
Hsp92II CATG 3 cut(s) 239, 391, 484
HspAI GCGC 2 cut(s) 166, 198
Kzo9I GATC 1 cut(s) 37
LpnPI CCDG 6 cut(s) 111, 171, 314, 387, 429, 489
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 3 cut(s) 57, 170, 400
MaeI CTAG 2 cut(s) 92, 571
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 3 cut(s) 73, 133, 226
MalI GATC 1 cut(s) 39
MboI GATC 1 cut(s) 37
MboII GAAGA 3 cut(s) 43, 419, 481
MfeI CAATTG 1 cut(s) 453
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 5 cut(s) 79, 205, 453, 461, 510
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 2 cut(s) 37, 371
MnlI CCTC 5 cut(s) 23, 26, 251, 297, 433
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 2 cut(s) 170, 335
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 212
MunI CAATTG 1 cut(s) 453
MvnI CGCG 1 cut(s) 166
NdeII GATC 1 cut(s) 37
NlaIII CATG 3 cut(s) 239, 391, 484
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 2 cut(s) 239, 484
OliI CACNNNNGTG 1 cut(s) 455
PciI ACATGT 1 cut(s) 480
PfeI GAWTC 2 cut(s) 86, 175
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 2 cut(s) 36, 370
PpsI GAGTC 2 cut(s) 36, 370
PscI ACATGT 1 cut(s) 480
PspPI GGNCC 3 cut(s) 10, 298, 424
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 2 cut(s) 170, 335
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 1 cut(s) 37
Sau96I GGNCC 3 cut(s) 10, 298, 424
SchI GAGTC 2 cut(s) 37, 371
SetI ASST 8 cut(s) 135, 160, 228, 312, 346, 353, 418, 492
SfaNI GCATC 3 cut(s) 57, 170, 400
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 4 cut(s) 5, 20, 41, 193
SmoI CTYRAG 4 cut(s) 5, 20, 41, 193
Sse9I AATT 5 cut(s) 79, 205, 453, 461, 510
SspI AATATT 1 cut(s) 286
SspMI CTAG 2 cut(s) 92, 571
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 346
TasI AATT 5 cut(s) 79, 205, 453, 461, 510
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 2 cut(s) 86, 175
Tru1I TTAA 2 cut(s) 170, 335
Tru9I TTAA 2 cut(s) 170, 335
TscAI CASTG 2 cut(s) 61, 235
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 111, 237, 555
TspRI CASTG 2 cut(s) 61, 235
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 4 cut(s) 79, 205, 461, 510
XbaI TCTAGA 1 cut(s) 91
XceI RCATGY 2 cut(s) 239, 484
XspI CTAG 2 cut(s) 92, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.