RLG00000021526

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019880
Physical Location & Seq
Reverse (-)
76198982 .. 76201483
2502 bp
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UTR
Exon/CDS
Intron
RLM00000021526

Sequence Viewer

Length: 702 bp
ATGACGACGATAGTCCAGACCTCCCGAGGAAGATCCGGCCCTCGCCGTCGTCTGAGCAAGCCTGGGCCGACGCCGGCGCTGAGGTTGCAGACGAGCATGTTAAGACTGTGTGACGAAGCCATACAATATCTTGAGTGCATCTTCACTGTCATCTGCAATGTTGTTACAAATTCTGATTGTCTAGATGAAGTATGTCAAATGGCAGAACTTATATCTGGAAAGGTTACACAGCAACCAAATGACAAACCTGCATCGCGCTTAAAGATTTTGTTCAATCTGTACAACCTACTGGAGGACCCATGTAGTCGGTTCTCTGTTTACTTGAGCGCTCTAAATTTGGCCATCAATGGAAAGGTCACTGAGCATGTCCTCCCTTCATTCAAACAGATAGATAGTTTCTTGAAGGAGTGGAATATTGGGGTCTTAGACCAGAGGCAGCTGTTTCTTACCATATCTAATGTTTTAAAAGAACATAAAAGCTTGGCAAAGGAGTCTTTCAAGTTCCTAACTAAGTACTTGGCAACATTATCTGGCGAAGATGCACCTGTTTTGGGCCAAGCCAAGGATGAGGCTGTTTGCACAATTGTGGAATTTGTGAAGACACCAGACATGTTTCAGCTTATTGTAATGTCTTGTGGAATTTCAAAGATGTTGCATAGCACTTTCGTAGTCTTATTTCTACTTTGTGCGTGTTCATCCTAG

Protein Analysis

234

Amino Acids

26.03

Weight (kDa)

8.31

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019880)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0165331 RchiOBHm_Chr2g0165451
rosa_laevigata RLG00000021526
rosa_multiflora Rmu_sc0000655.1_g000019
rosa_samantha Rh2BG594100
rosa_wichuraiana Rw2G048440 Rw2G048490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
AccII CGCG 1 cut(s) 256
AclWI GGATC 1 cut(s) 27
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 4 cut(s) 169, 334, 590, 639
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 2 cut(s) 281, 515
AfeI AGCGCT 1 cut(s) 328
AfiI CCNNNNNNNGG 3 cut(s) 292, 551, 562
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 5 cut(s) 274, 382, 403, 499, 645
AjnI CCWGG 1 cut(s) 61
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 3 cut(s) 439, 480, 619
AluI AGCT 3 cut(s) 439, 480, 619
AlwI GGATC 1 cut(s) 27
Ama87I CYCGRG 1 cut(s) 24
Aor51HI AGCGCT 1 cut(s) 328
AoxI GGCC 4 cut(s) 37, 65, 339, 553
ApeKI GCWGC 1 cut(s) 436
ApoI RAATTY 4 cut(s) 169, 334, 590, 639
AspLEI GCGC 3 cut(s) 79, 258, 329
AspS9I GGNCC 4 cut(s) 38, 65, 295, 553
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 448
BccI CCATC 1 cut(s) 350
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 63
BfaI CTAG 2 cut(s) 182, 700
BfoI RGCGCY 2 cut(s) 80, 330
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 1 cut(s) 437
BlsI GCNGC 1 cut(s) 438
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 4 cut(s) 38, 65, 295, 553
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 3 cut(s) 147, 260, 529
BoxI GACNNNNGTC 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 152, 343
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 3 cut(s) 25, 62, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 551, 562
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 3 cut(s) 25, 62, 561
BseGI GGATG 2 cut(s) 571, 695
BseLI CCNNNNNNNGG 3 cut(s) 292, 551, 562
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 3 cut(s) 44, 71, 351
BseNI ACTGG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 4 cut(s) 39, 67, 341, 555
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 36, 74
BslI CCNNNNNNNGG 3 cut(s) 292, 551, 562
BsnI GGCC 4 cut(s) 39, 67, 341, 555
BsoBI CYCGRG 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 1 cut(s) 32
BspANI GGCC 4 cut(s) 39, 67, 341, 555
BspCNI CTCAG 3 cut(s) 45, 72, 352
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 25, 62, 561
BssMI GATC 1 cut(s) 32
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 2 cut(s) 108, 148
BstACI GRCGYC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 59, 75
BstDEI CTNAG 5 cut(s) 53, 80, 360, 424, 510
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 571, 695
BstFNI CGCG 1 cut(s) 256
BstH2I RGCGCY 2 cut(s) 80, 330
BstHHI GCGC 3 cut(s) 79, 258, 329
BstKTI GATC 1 cut(s) 35
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstNI CCWGG 1 cut(s) 63
BstNSI RCATGY 3 cut(s) 100, 368, 613
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 61
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 448
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 4 cut(s) 39, 67, 341, 555
BtgZI GCGATG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 571, 695
BtsIMutI CAGTG 2 cut(s) 144, 357
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 59, 75
CfoI GCGC 3 cut(s) 79, 258, 329
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 4 cut(s) 38, 65, 295, 553
CseI GACGC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 280, 514
CviAII CATG 4 cut(s) 97, 300, 365, 610
CviQI GTAC 2 cut(s) 280, 514
DdeI CTNAG 5 cut(s) 53, 80, 360, 424, 510
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 295
Eco47III AGCGCT 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 4 cut(s) 100, 303, 368, 613
FatI CATG 4 cut(s) 96, 299, 364, 609
Fnu4HI GCNGC 1 cut(s) 437
FokI GGATG 2 cut(s) 578, 682
Fsp4HI GCNGC 1 cut(s) 437
FspBI CTAG 2 cut(s) 182, 700
GlaI GCGC 3 cut(s) 78, 257, 328
GluI GCNGC 1 cut(s) 437
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 2 cut(s) 80, 330
HaeIII GGCC 4 cut(s) 39, 67, 341, 555
HapII CCGG 2 cut(s) 36, 74
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 79, 258, 329
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 4 cut(s) 100, 303, 368, 613
Hin6I GCGC 3 cut(s) 77, 256, 327
HinP1I GCGC 3 cut(s) 77, 256, 327
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 1 cut(s) 491
HpaII CCGG 2 cut(s) 36, 74
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 2 cut(s) 54, 175
Hpy188III TCNNGA 6 cut(s) 16, 24, 131, 182, 216, 400
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 3 cut(s) 10, 51, 73
HpyAV CCTTC 2 cut(s) 384, 397
HpyCH4III ACNGT 2 cut(s) 108, 148
HpyCH4V TGCA 7 cut(s) 88, 138, 156, 251, 542, 579, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 5 cut(s) 53, 80, 360, 424, 510
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 4 cut(s) 100, 303, 368, 613
HspAI GCGC 3 cut(s) 77, 256, 327
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Kzo9I GATC 1 cut(s) 32
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 3 cut(s) 147, 260, 529
MaeI CTAG 2 cut(s) 182, 700
MaeIII GTNAC 4 cut(s) 110, 163, 223, 355
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 4 cut(s) 42, 133, 548, 610
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 5 cut(s) 169, 334, 582, 590, 639
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 8 cut(s) 20, 31, 51, 75, 286, 380, 426, 562
Mox20I TGGCCA 1 cut(s) 341
MreI CGCCGGCG 1 cut(s) 73
MroNI GCCGGC 1 cut(s) 73
MscI TGGCCA 1 cut(s) 341
MseI TTAA 3 cut(s) 101, 260, 464
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 2 cut(s) 36, 74
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 85
NaeI GCCGGC 1 cut(s) 75
NdeII GATC 1 cut(s) 32
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 4 cut(s) 100, 303, 368, 613
NlaIV GGNNCC 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 110, 355
NspI RCATGY 3 cut(s) 100, 368, 613
OliI CACNNNNGTG 1 cut(s) 584
PciI ACATGT 1 cut(s) 609
PdiI GCCGGC 1 cut(s) 75
PkrI GCNGC 1 cut(s) 438
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PpuMI RGGWCCY 1 cut(s) 295
PscI ACATGT 1 cut(s) 609
PshAI GACNNNNGTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 4 cut(s) 38, 65, 295, 553
PspPPI RGGWCCY 1 cut(s) 295
PsuI RGATCY 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 439
RsaI GTAC 2 cut(s) 281, 515
RsaNI GTAC 2 cut(s) 280, 514
RseI CAYNNNNRTG 1 cut(s) 584
SaqAI TTAA 3 cut(s) 101, 260, 464
SatI GCNGC 1 cut(s) 437
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 4 cut(s) 38, 65, 295, 553
ScaI AGTACT 1 cut(s) 515
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 63
SfaNI GCATC 3 cut(s) 147, 260, 529
SgrAI CRCCGGYG 1 cut(s) 73
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 131, 322
SmoI CTYRAG 2 cut(s) 131, 322
Sse9I AATT 5 cut(s) 169, 334, 582, 590, 639
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 182, 700
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 108, 148
TasI AATT 5 cut(s) 169, 334, 582, 590, 639
TatI WGTACW 2 cut(s) 279, 513
Tru1I TTAA 3 cut(s) 101, 260, 464
Tru9I TTAA 3 cut(s) 101, 260, 464
TscAI CASTG 2 cut(s) 151, 364
TseFI GTSAC 2 cut(s) 110, 355
TseI GCWGC 1 cut(s) 436
Tsp45I GTSAC 2 cut(s) 110, 355
TspDTI ATGAA 3 cut(s) 201, 366, 684
TspRI CASTG 2 cut(s) 151, 364
VpaK11BI GGWCC 1 cut(s) 295
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 4 cut(s) 169, 334, 590, 639
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 3 cut(s) 100, 368, 613
XspI CTAG 2 cut(s) 182, 700
ZrmI AGTACT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.