Rh2BG594100

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0019880
Physical Location & Seq
Reverse (-)
81353250 .. 81400465
47216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG594100.1

Sequence Viewer

Length: 669 bp
ATGGAAGGACCGGAGTCAAGAGCTGTCACATATGGATTTGGGTTGCAGGTGAGCATGTTAAAAGACTGTGAGGAAGCCATACAATGTGTGGAAAATGCCAGATGGTTCGATTTGGTGTCACTTATGCTCACTTCAGCTGATGTGATACTATCAAAGGTCTCAGACACAGATCTTGAGTGCATCTTCACTGTCATCTGCAATGTTGGTACAAATTCTGATTGTCTAGATGAAGTATGTCAAATGGCAGAACTTATATATGGAAAGGTTACACAGCAACCAAATGACAAACCTGCATCGCGCTTAAAGATTTTGTTCAATCTGTACAACCTACTGGAGGACCCATGTAGTCGGTTCTCTGTTTACTTGAGCGCTCTAAATTTGGCCATCAATGGAAAGGTCACTGAGCACGTCCTCCCTTCATTCAAACAGATAGATTGTTTCTTGAAGGAGTGGAATATTGGGGTCTTGGACCAGAGGCAGCTATTTCTTACCATATCTAATGTTTTAAAAGAACGTAAAAGCTTGGCAAAGGAGTCTTTCAAGTTCCTAACTAAGCACTTGGCAACATTATCTGGCGAAGATGCACCTGTTTTGGGCCAAGCCAAGGATGAGGCACTTGGCAACATTATCTGGCGAAGATTCACCTGTTTTGAGCCAAGCCAAGGATGA

Protein Analysis

222

Amino Acids

24.91

Weight (kDa)

4.97

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019880)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0165331 RchiOBHm_Chr2g0165451
rosa_laevigata RLG00000021526
rosa_multiflora Rmu_sc0000655.1_g000019
rosa_samantha Rh2BG594100
rosa_wichuraiana Rw2G048440 Rw2G048490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 2 cut(s) 37, 298
AccII CGCG 1 cut(s) 298
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 2 cut(s) 211, 376
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 2 cut(s) 208, 323
AfeI AGCGCT 1 cut(s) 370
AfiI CCNNNNNNNGG 4 cut(s) 334, 593, 604, 662
AgsI TTSAA 4 cut(s) 316, 424, 445, 541
AjiI CACGTC 1 cut(s) 409
AluBI AGCT 4 cut(s) 23, 137, 481, 522
AluI AGCT 4 cut(s) 23, 137, 481, 522
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 163
Aor51HI AGCGCT 1 cut(s) 370
AoxI GGCC 2 cut(s) 381, 595
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 2 cut(s) 211, 376
AspLEI GCGC 2 cut(s) 300, 371
AspS9I GGNCC 4 cut(s) 8, 337, 469, 595
AsuHPI GGTGA 2 cut(s) 61, 634
AvaII GGWCC 3 cut(s) 8, 337, 469
BalI TGGCCA 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 96, 392
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 2 cut(s) 37, 298
BglII AGATCT 1 cut(s) 169
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
Bme18I GGWCC 3 cut(s) 8, 337, 469
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 4 cut(s) 8, 337, 469, 595
BmiI GGNNCC 1 cut(s) 339
BmsI GCATC 3 cut(s) 189, 302, 571
BoxI GACNNNNGTC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 194, 385
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 603, 661
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 593, 604, 662
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 205
BseDI CCNNGG 2 cut(s) 603, 661
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 4 cut(s) 334, 593, 604, 662
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 174, 393
BseNI ACTGG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 490
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 383, 597
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 11
BslI CCNNNNNNNGG 4 cut(s) 334, 593, 604, 662
BsmAI GTCTC 1 cut(s) 163
BsnI GGCC 2 cut(s) 383, 597
Bso31I GGTCTC 1 cut(s) 163
Bsp1286I GDGCHC 1 cut(s) 408
Bsp1407I TGTACA 1 cut(s) 321
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 2 cut(s) 383, 597
BspCNI CTCAG 2 cut(s) 173, 394
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 339
BspMI ACCTGC 2 cut(s) 37, 298
BspTNI GGTCTC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 603, 661
BssMI GATC 1 cut(s) 169
BssT1I CCWWGG 2 cut(s) 603, 661
Bst4CI ACNGT 2 cut(s) 68, 190
BstAUI TGTACA 1 cut(s) 321
BstDEI CTNAG 3 cut(s) 160, 402, 552
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 298
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 300, 371
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 58
BstPAI GACNNNNGTC 1 cut(s) 13
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 2 cut(s) 383, 597
BtgZI GCGATG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 186, 399
BveI ACCTGC 2 cut(s) 37, 298
CfoI GCGC 2 cut(s) 300, 371
Cfr13I GGNCC 4 cut(s) 8, 337, 469, 595
Csp6I GTAC 2 cut(s) 207, 322
CviAII CATG 2 cut(s) 55, 342
CviQI GTAC 2 cut(s) 207, 322
DdeI CTNAG 3 cut(s) 160, 402, 552
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
DraI TTTAAA 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 603, 661
Eco31I GGTCTC 1 cut(s) 163
Eco47I GGWCC 3 cut(s) 8, 337, 469
Eco47III AGCGCT 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 117
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 603, 661
ErhI CCWWGG 2 cut(s) 603, 661
FaeI CATG 2 cut(s) 58, 345
FatI CATG 2 cut(s) 54, 341
FauNDI CATATG 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 1 cut(s) 479
FspBI CTAG 1 cut(s) 224
GlaI GCGC 2 cut(s) 299, 370
GluI GCNGC 1 cut(s) 479
GsuI CTGGAG 1 cut(s) 353
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 383, 597
HapII CCGG 1 cut(s) 11
HhaI GCGC 2 cut(s) 300, 371
Hin1II CATG 2 cut(s) 58, 345
Hin6I GCGC 2 cut(s) 298, 369
HinP1I GCGC 2 cut(s) 298, 369
HindIII AAGCTT 1 cut(s) 520
HinfI GANTC 3 cut(s) 14, 533, 639
HpaII CCGG 1 cut(s) 11
HphI GGTGA 2 cut(s) 61, 634
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 163, 217
Hpy188III TCNNGA 4 cut(s) 18, 173, 224, 442
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 2 cut(s) 426, 439
HpyCH4III ACNGT 2 cut(s) 68, 190
HpyCH4IV ACGT 2 cut(s) 408, 514
HpyCH4V TGCA 5 cut(s) 46, 180, 198, 293, 584
HpyF3I CTNAG 3 cut(s) 160, 402, 552
HpySE526I ACGT 2 cut(s) 408, 514
Hsp92II CATG 2 cut(s) 58, 345
HspAI GCGC 2 cut(s) 298, 369
Kzo9I GATC 1 cut(s) 169
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 3 cut(s) 189, 302, 571
MaeI CTAG 1 cut(s) 224
MaeII ACGT 2 cut(s) 408, 514
MaeIII GTNAC 4 cut(s) 25, 117, 265, 397
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 3 cut(s) 175, 590, 648
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 1 cut(s) 408
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 2 cut(s) 211, 376
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 2 cut(s) 23, 542
MnlI CCTC 5 cut(s) 64, 328, 422, 468, 604
Mox20I TGGCCA 1 cut(s) 383
MscI TGGCCA 1 cut(s) 383
MseI TTAA 3 cut(s) 59, 302, 506
Msp20I TGGCCA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 298
NdeI CATATG 1 cut(s) 31
NdeII GATC 1 cut(s) 169
NlaIII CATG 2 cut(s) 58, 345
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 3 cut(s) 25, 117, 397
NspI RCATGY 1 cut(s) 58
PaqCI CACCTGC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 639
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 2 cut(s) 22, 541
PpsI GAGTC 2 cut(s) 22, 541
PpuMI RGGWCCY 1 cut(s) 337
PshAI GACNNNNGTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 4 cut(s) 8, 337, 469, 595
PspPPI RGGWCCY 1 cut(s) 337
PsuI RGATCY 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 208, 323
RsaNI GTAC 2 cut(s) 207, 322
SaqAI TTAA 3 cut(s) 59, 302, 506
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 4 cut(s) 8, 337, 469, 595
SchI GAGTC 2 cut(s) 23, 542
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 3 cut(s) 189, 302, 571
SinI GGWCC 3 cut(s) 8, 337, 469
SmlI CTYRAG 2 cut(s) 173, 364
SmoI CTYRAG 2 cut(s) 173, 364
Sse9I AATT 2 cut(s) 211, 376
SspI AATATT 1 cut(s) 457
SspMI CTAG 1 cut(s) 224
StyI CCWWGG 2 cut(s) 603, 661
TaaI ACNGT 2 cut(s) 68, 190
TaiI ACGT 2 cut(s) 411, 517
TaqI TCGA 1 cut(s) 108
TasI AATT 2 cut(s) 211, 376
TatI WGTACW 1 cut(s) 321
TfiI GAWTC 1 cut(s) 639
Tru1I TTAA 3 cut(s) 59, 302, 506
Tru9I TTAA 3 cut(s) 59, 302, 506
TscAI CASTG 2 cut(s) 193, 406
TseFI GTSAC 3 cut(s) 25, 117, 397
TseI GCWGC 1 cut(s) 478
Tsp45I GTSAC 3 cut(s) 25, 117, 397
TspDTI ATGAA 2 cut(s) 243, 408
TspRI CASTG 2 cut(s) 193, 406
VpaK11BI GGWCC 3 cut(s) 8, 337, 469
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 2 cut(s) 211, 376
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.