RchiOBHm_Chr2g0176761

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018332
Physical Location & Seq
Forward (+)
88481994 .. 88483926
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54376

Sequence Viewer

Length: 1305 bp
ATGATGGTTGCATGCCTTTCATGTACTCCTGAGGTTCTACCCAAAAGTAGCCCACTCACCAGCTGTAGACAAACCAAATTTGGTTCTCAGGAAGCTCTCATTTTACTCCGAAACTGCACCACTTTCAATCGTCTCAAGCAAATTCATGCTAAGATCATCCGCAGTAGTCTTTCTCGAGACCAATTAATTATGAGGAAACTCATTCAACTTTGCTCTTCCCATGGGAAAATGGACTATGCCACTCTTGTATTTCATCAAATTCAGGGTCACCTTACCTTCACTTGGAATCTAATGATTATGTCTTATACCATCAATGGGTGCTCACAACAAGCCCTCCTTCTGTATAGCCATATGATACGCCAAGGAGTTCCACCTGATAAGTTCACCTTTCCATTTGTTATTAAAGCTTGCATTTCTTCTACTGCATTTGAACTAGGAAAAGTGGTTCATGGGCTAGTGATCAAAAATAGTTTCTCAGGAGATATATTCATACAAAATACTTTGATGGACTTCTACTTCAAGTCCGGACAAATAGATTGTGGTTCCAGGGTGTTTGAAAAAATGCGGGTTCGCAGTGTGGTTTCATGGACAACCATGATTTCGGGGTTAGTTGCTTGTGGGAAGTTATCTGCTGCCCGAGGGGTTTTTGAGCGAATGCCAGCTAAAAATGTTGTTTCTTGGACTGCAATGATCAATGGGTATGTCAAAAACCAACAACCTGAAGAGGCATTTGAACTGTTCTGGAGAATGCAGCTTGGTAATGTTAGGCCAAATGAGTTCACGCTGGTAAGCTTGCTGAAAGCATGTACCCAATTCGAAAGCCTCAAGTTGGCTAGACGGATTCATGACTTTGCTCTTAAAAACGGGTTTAAAATTGATGTTTTTCTTGGCACAGCACTTATTGATACTTATAGCAAATGTGGTAGTTTAGAGGATGCAAAGAGAGTGTTTGATAAGATGCAAATCAAGAGCTTGGCTACTTGGAATTCAATGATCACTAGCTTGGGTGTGCATGGGTTTGGAGAGGAAGCCCTTGCCCTTTTTGCAGCGATGGAAGAGGCGAATGTACGGCCAGATGCAATCACCTTTGTAGGTGTTTTATCTGCTTGTTTGCATACCAATAATCTGGAGGATGGTTGCAGACATTTCAAATACATGAGTGAACGTTATGGTATCACACCTATCTTAGAGAATTATACTTGCATGATTGAACTGTATAGCCGTTTTGATATGTTAGATGAGGTATGCAAATTAGTAGAAGCCATGCCTGTAAAGCCTAATAGAACTAAGGAACATCATCTTTAG

Protein Analysis

434

Amino Acids

49.13

Weight (kDa)

9.01

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 137 1.3e-08 PPR repeat family
PPR PF01535 194 - 223 6.7e-06 PPR repeat
PPR_2 PF13041 222 - 269 2.4e-13 PPR repeat family
PPR PF01535 225 - 251 5.6e-08 PPR repeat
PPR_2 PF13041 325 - 371 6.3e-08 PPR repeat family
PPR PF01535 327 - 355 5.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26630
prunus_persica Prupe.3G315700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0176761
rosa_laevigata RLG00000022444
rosa_roxburghii Rroxscaffold_2G00076180
rosa_samantha Rh2AG675900 Rh2BG687000 Rh2CG650400 Rh2DG701300
rosa_wichuraiana Rw2G055460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 67
AccIII TCCGGA 1 cut(s) 524
AciI CCGC 2 cut(s) 160, 565
AclI AACGTT 1 cut(s) 1165
AcoI YGGCCR 1 cut(s) 1070
AcsI RAATTY 4 cut(s) 77, 141, 258, 985
AcuI CTGAAG 1 cut(s) 741
AfaI GTAC 3 cut(s) 25, 808, 1068
AfiI CCNNNNNNNGG 3 cut(s) 282, 315, 829
AgsI TTSAA 9 cut(s) 127, 206, 431, 520, 557, 734, 990, 1150, 1211
AjnI CCWGG 1 cut(s) 545
AloI GAACNNNNNNTCC 2 cut(s) 429, 461
AluBI AGCT 8 cut(s) 63, 95, 407, 662, 754, 792, 972, 1002
AluI AGCT 8 cut(s) 63, 95, 407, 662, 754, 792, 972, 1002
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 137, 171
Ama87I CYCGRG 2 cut(s) 174, 636
Aor13HI TCCGGA 1 cut(s) 524
AoxI GGCC 2 cut(s) 767, 1070
ApeKI GCWGC 3 cut(s) 632, 751, 1046
ApoI RAATTY 4 cut(s) 77, 141, 258, 985
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AseI ATTAAT 1 cut(s) 185
AsuHPI GGTGA 4 cut(s) 49, 260, 376, 1075
AsuII TTCGAA 1 cut(s) 816
AvaI CYCGRG 2 cut(s) 174, 636
AxyI CCTNAGG 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 3 cut(s) 619, 763, 1058
BccI CCATC 4 cut(s) 317, 499, 1045, 1127
BceAI ACGGC 2 cut(s) 1085, 1206
BciT130I CCWGG 1 cut(s) 547
BclI TGATCA 3 cut(s) 459, 690, 993
BcoDI GTCTC 2 cut(s) 137, 171
BfaI CTAG 4 cut(s) 434, 455, 834, 999
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 3 cut(s) 633, 752, 1047
BlsI GCNGC 3 cut(s) 634, 753, 1048
Bme1390I CCNGG 1 cut(s) 547
BmeT110I CYCGRG 2 cut(s) 174, 636
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 925, 948, 1066
BpmI CTGGAG 2 cut(s) 763, 1148
Bpu14I TTCGAA 1 cut(s) 816
BpuEI CTTGAG 2 cut(s) 119, 809
BsaBI GATNNNNATC 1 cut(s) 962
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 4 cut(s) 220, 361, 546, 637
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 315, 829
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 962
BseAI TCCGGA 1 cut(s) 524
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 4 cut(s) 220, 361, 546, 637
BseGI GGATG 3 cut(s) 156, 940, 1138
BseJI GATNNNNATC 1 cut(s) 962
BseLI CCNNNNNNNGG 3 cut(s) 282, 315, 829
BseMI GCAATG 1 cut(s) 693
BseMII CTCAG 3 cut(s) 21, 101, 489
BseXI GCAGC 3 cut(s) 619, 763, 1058
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 2 cut(s) 769, 1072
BsiHKAI GWGCWC 1 cut(s) 323
BsiHKCI CYCGRG 2 cut(s) 174, 636
BsiSI CCGG 1 cut(s) 525
BslI CCNNNNNNNGG 3 cut(s) 282, 315, 829
BsmAI GTCTC 2 cut(s) 137, 171
BsmBI CGTCTC 1 cut(s) 137
BsmI GAATGC 2 cut(s) 660, 753
BsnI GGCC 2 cut(s) 769, 1072
Bso31I GGTCTC 1 cut(s) 171
BsoBI CYCGRG 2 cut(s) 174, 636
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 524
Bsp143I GATC 4 cut(s) 153, 459, 690, 993
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 2 cut(s) 160, 565
BspANI GGCC 2 cut(s) 769, 1072
BspCNI CTCAG 3 cut(s) 22, 100, 488
BspEI TCCGGA 1 cut(s) 524
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 1 cut(s) 544
BspQI GCTCTTC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 816
BspTNI GGTCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 693
BssECI CCNNGG 4 cut(s) 220, 361, 546, 637
BssMI GATC 4 cut(s) 153, 459, 690, 993
BssT1I CCWWGG 2 cut(s) 220, 361
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 2 cut(s) 738, 1215
Bst6I CTCTTC 3 cut(s) 220, 717, 1050
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 4 cut(s) 13, 409, 660, 794
BstDEI CTNAG 6 cut(s) 30, 87, 150, 475, 1186, 1287
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 266
BstF5I GGATG 3 cut(s) 156, 940, 1138
BstKTI GATC 4 cut(s) 156, 462, 693, 996
BstMAI GTCTC 2 cut(s) 137, 171
BstMBI GATC 4 cut(s) 153, 459, 690, 993
BstMWI GCNNNNNNNGC 2 cut(s) 1043, 1273
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 2 cut(s) 15, 807
BstPI GGTNACC 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 619, 763, 1058
BstXI CCANNNNNNTGG 1 cut(s) 1126
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 2 cut(s) 769, 1072
BtgI CCRYGG 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 1064
BtsCI GGATG 3 cut(s) 156, 940, 1138
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 1 cut(s) 580
Cac8I GCNNGC 4 cut(s) 13, 409, 660, 794
CciI TCATGA 1 cut(s) 844
Csp6I GTAC 3 cut(s) 24, 807, 1067
CviQI GTAC 3 cut(s) 24, 807, 1067
DdeI CTNAG 6 cut(s) 30, 87, 150, 475, 1186, 1287
DpnI GATC 4 cut(s) 155, 461, 692, 995
DpnII GATC 4 cut(s) 153, 459, 690, 993
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 1070
Eam1104I CTCTTC 3 cut(s) 220, 717, 1050
EarI CTCTTC 3 cut(s) 220, 717, 1050
Eco130I CCWWGG 2 cut(s) 220, 361
Eco31I GGTCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 741
Eco81I CCTNAGG 1 cut(s) 30
Eco88I CYCGRG 2 cut(s) 174, 636
Eco91I GGTNACC 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 266
EcoRI GAATTC 1 cut(s) 985
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 2 cut(s) 220, 361
ErhI CCWWGG 2 cut(s) 220, 361
Esp3I CGTCTC 1 cut(s) 137
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 371, 403
FauI CCCGC 1 cut(s) 558
FauNDI CATATG 1 cut(s) 351
FbaI TGATCA 3 cut(s) 459, 690, 993
FblI GTMKAC 1 cut(s) 67
Fnu4HI GCNGC 3 cut(s) 633, 752, 1047
FokI GGATG 3 cut(s) 143, 947, 1145
Fsp4HI GCNGC 3 cut(s) 633, 752, 1047
FspBI CTAG 4 cut(s) 434, 455, 834, 999
GluI GCNGC 3 cut(s) 633, 752, 1047
GsuI CTGGAG 2 cut(s) 763, 1148
HaeIII GGCC 2 cut(s) 769, 1072
HapII CCGG 1 cut(s) 525
HindIII AAGCTT 2 cut(s) 405, 790
HinfI GANTC 2 cut(s) 286, 841
HpaII CCGG 1 cut(s) 525
HphI GGTGA 4 cut(s) 49, 260, 376, 1075
Hpy166II GTNNAC 4 cut(s) 68, 384, 780, 1163
Hpy188I TCNGA 1 cut(s) 110
Hpy8I GTNNAC 4 cut(s) 68, 384, 780, 1163
HpyAV CCTTC 2 cut(s) 286, 347
HpyCH4III ACNGT 2 cut(s) 738, 1215
HpyCH4IV ACGT 1 cut(s) 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 1043, 1273
HpyF3I CTNAG 6 cut(s) 30, 87, 150, 475, 1186, 1287
HpySE526I ACGT 1 cut(s) 1165
Kpn2I TCCGGA 1 cut(s) 524
Ksp22I TGATCA 3 cut(s) 459, 690, 993
Kzo9I GATC 4 cut(s) 153, 459, 690, 993
LguI GCTCTTC 1 cut(s) 220
Lsp1109I GCAGC 3 cut(s) 619, 763, 1058
LweI GCATC 3 cut(s) 925, 948, 1066
MaeI CTAG 4 cut(s) 434, 455, 834, 999
MaeII ACGT 1 cut(s) 1165
MaeIII GTNAC 1 cut(s) 266
MalI GATC 4 cut(s) 155, 461, 692, 995
MboI GATC 4 cut(s) 153, 459, 690, 993
MboII GAAGA 4 cut(s) 207, 408, 734, 1067
MhlI GDGCHC 1 cut(s) 323
MroI TCCGGA 1 cut(s) 524
MseI TTAA 4 cut(s) 185, 402, 858, 870
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 547
Mva1269I GAATGC 2 cut(s) 660, 753
MvaI CCWGG 1 cut(s) 547
MwoI GCNNNNNNNGC 2 cut(s) 1043, 1273
NcoI CCATGG 1 cut(s) 220
NdeI CATATG 1 cut(s) 351
NdeII GATC 4 cut(s) 153, 459, 690, 993
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 1 cut(s) 266
NspI RCATGY 2 cut(s) 15, 807
NspV TTCGAA 1 cut(s) 816
PaeI GCATGC 1 cut(s) 15
PaeR7I CTCGAG 1 cut(s) 174
PagI TCATGA 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 220
PctI GAATGC 2 cut(s) 660, 753
PfeI GAWTC 2 cut(s) 286, 841
PkrI GCNGC 3 cut(s) 634, 753, 1048
PshBI ATTAAT 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 1165
Psp6I CCWGG 1 cut(s) 545
PspEI GGTNACC 1 cut(s) 266
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 63
RsaI GTAC 3 cut(s) 25, 808, 1068
RsaNI GTAC 3 cut(s) 24, 807, 1067
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 4 cut(s) 185, 402, 858, 870
SatI GCNGC 3 cut(s) 633, 752, 1047
Sau3AI GATC 4 cut(s) 153, 459, 690, 993
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 3 cut(s) 925, 948, 1066
SfcI CTRYAG 1 cut(s) 64
Sfr274I CTCGAG 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 816
SlaI CTCGAG 1 cut(s) 174
SmlI CTYRAG 3 cut(s) 134, 174, 824
SmoI CTYRAG 3 cut(s) 134, 174, 824
SphI GCATGC 1 cut(s) 15
SsiI CCGC 2 cut(s) 160, 565
SspMI CTAG 4 cut(s) 434, 455, 834, 999
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 2 cut(s) 220, 361
TaaI ACNGT 2 cut(s) 738, 1215
TaiI ACGT 1 cut(s) 1168
TaqI TCGA 2 cut(s) 175, 816
TatI WGTACW 1 cut(s) 23
TfiI GAWTC 2 cut(s) 286, 841
Tru1I TTAA 4 cut(s) 185, 402, 858, 870
Tru9I TTAA 4 cut(s) 185, 402, 858, 870
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 3 cut(s) 632, 751, 1046
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 7 cut(s) 9, 134, 242, 437, 478, 573, 833
TspGWI ACGGA 1 cut(s) 853
TspRI CASTG 1 cut(s) 580
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 4 cut(s) 77, 141, 258, 985
XceI RCATGY 2 cut(s) 15, 807
XhoI CTCGAG 1 cut(s) 174
XmiI GTMKAC 1 cut(s) 67
XspI CTAG 4 cut(s) 434, 455, 834, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.