RchiOBHm_Chr3g0459121

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0019916
Physical Location & Seq
Reverse (-)
7626764 .. 7627668
905 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42572

Sequence Viewer

Length: 858 bp
ATGTGTTTCAGATTTTTTCGAATTCGGAACATTGCAACCCTTACACTTAATACTGTTATCATCAAGGCACTGGTTGCCGACTCTGACCCTAGTTTTCTTGGGGTTGCCAAGGAGTGTGTCATGGAGATGATGGGCAAGGGACTGCAGCCCAGTGCCAGCTCATACACTGCCGTGTATGAGGCGTTTTCTCGGCAAGAGAAAATGGAGGAGGCCAAGCAGTTTCTTGGGGAGATGAAAGCCAAGGGGTTTGTGGCTGACAACAAGGCAATGAGGGCTATGAGACTGGTTCTTAAGGGCAAAGGAGCTGTGATCAAAAGCATCACCGACATTCTCTTCTCAGATGAGTTAGAAGACTACTCAGACGACTCAGATGATGATGATGAAATACAGAACTACAGCGAAGAGATGGAATCGAGGTTCCCTCCGGATAGTTATTTATCTTTCAGCAAGAAACATGTCATCGAGGATGACCCAGAAAACCCCAAAGATTTGCAACAAGTCTTCCATAAGATGCGCAAAGGAAGACCTGTCATCAATATTTATTTTCACCAAGATGATCATCATGCTAAAGAAAGATGGGTTCTTCTGCCAGGTCCAGGAGTGACACACACCGGCATCATGGGGATCTATGCCAAGGCCGATGAGGTCAAGGAGGTTCTCAAAGTGTTCATGCGCATGCTATCTCTTGGAGTCGCTCCCAACGCATACACTAACAGTGTTCTCATTAAGGCACTTGCAAAAGACCCCAATTTCCTTGGTGATGTCAAGATGTACACTCTAGAAATGATGGACAAGGGAATGCGGCCGAATGCCAGGACCTACACTGCCGTGTTCAAGGCCTTTGCCCGGCTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

285

Amino Acids

32.35

Weight (kDa)

6.34

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 201 - 247 1.4e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019916)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0459121
rosa_rugosa Rorug03G0033500
rosa_samantha Rh1DG011000 Rh3BG095000 Rh3CG095300 Rh3DG095100 Rh3DG095700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 515, 674
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 1 cut(s) 802
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 1 cut(s) 21
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 2 cut(s) 596, 846
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 1 cut(s) 835
AjnI CCWGG 3 cut(s) 589, 595, 812
AleI CACNNNNGTG 2 cut(s) 170, 827
AloI GAACNNNNNNTCC 2 cut(s) 401, 433
AluBI AGCT 2 cut(s) 159, 305
AluI AGCT 2 cut(s) 159, 305
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 4 cut(s) 210, 636, 803, 837
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 21
AspLEI GCGC 2 cut(s) 516, 675
AspS9I GGNCC 2 cut(s) 593, 816
AsuC2I CCSGG 1 cut(s) 847
AsuHPI GGTGA 3 cut(s) 313, 539, 770
AsuII TTCGAA 1 cut(s) 19
AvaII GGWCC 2 cut(s) 593, 816
BbsI GAAGAC 3 cut(s) 357, 493, 529
BbvI GCAGC 1 cut(s) 157
BccI CCATC 4 cut(s) 124, 400, 570, 781
BceAI ACGGC 2 cut(s) 155, 812
BciT130I CCWGG 3 cut(s) 591, 597, 814
BclI TGATCA 2 cut(s) 309, 556
BcnI CCSGG 1 cut(s) 847
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 2 cut(s) 90, 779
BfmI CTRYAG 2 cut(s) 143, 394
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 2 cut(s) 146, 803
BlsI GCNGC 2 cut(s) 147, 804
Bme1390I CCNGG 4 cut(s) 591, 597, 814, 847
Bme18I GGWCC 2 cut(s) 593, 816
BmgT120I GGNCC 2 cut(s) 593, 816
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 4 cut(s) 591, 597, 814, 847
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 3 cut(s) 327, 501, 624
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 3 cut(s) 357, 493, 529
BplI GAGNNNNNCTC 2 cut(s) 406, 438
Bpu14I TTCGAA 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 847
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 4 cut(s) 108, 240, 633, 754
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 846
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 3 cut(s) 75, 150, 288
Bse3DI GCAATG 2 cut(s) 30, 273
Bse8I GATNNNNATC 1 cut(s) 558
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 3 cut(s) 591, 597, 814
BseDI CCNNGG 4 cut(s) 108, 240, 633, 754
BseGI GGATG 1 cut(s) 472
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 2 cut(s) 596, 846
BseMI GCAATG 2 cut(s) 30, 273
BseMII CTCAG 3 cut(s) 351, 372, 381
BseNI ACTGG 3 cut(s) 75, 150, 288
BseRI GAGGAG 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 803
BseXI GCAGC 1 cut(s) 157
Bsh1285I CGRYCG 1 cut(s) 806
BshFI GGCC 4 cut(s) 212, 638, 805, 839
BsiEI CGRYCG 1 cut(s) 806
BsiSI CCGG 3 cut(s) 425, 612, 847
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 2 cut(s) 596, 846
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 153
BsmI GAATGC 2 cut(s) 804, 814
BsnI GGCC 4 cut(s) 212, 638, 805, 839
Bsp119I TTCGAA 1 cut(s) 19
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 3 cut(s) 309, 556, 624
BspACI CCGC 1 cut(s) 802
BspANI GGCC 4 cut(s) 212, 638, 805, 839
BspCNI CTCAG 3 cut(s) 350, 371, 380
BspEI TCCGGA 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 147
BspPI GGATC 1 cut(s) 632
BspT104I TTCGAA 1 cut(s) 19
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 30, 273
BsrFI RCCGGY 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 3 cut(s) 75, 150, 288
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 4 cut(s) 108, 240, 633, 754
BssMI GATC 3 cut(s) 309, 556, 624
BssT1I CCWWGG 4 cut(s) 108, 240, 633, 754
Bst2UI CCWGG 3 cut(s) 591, 597, 814
Bst4CI ACNGT 2 cut(s) 55, 716
Bst6I CTCTTC 2 cut(s) 338, 396
BstAFI CTTAAG 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 74
BstAUI TGTACA 1 cut(s) 771
BstBI TTCGAA 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 157, 677
BstDEI CTNAG 3 cut(s) 337, 358, 367
BstF5I GGATG 1 cut(s) 472
BstHHI GCGC 2 cut(s) 516, 675
BstKTI GATC 3 cut(s) 312, 559, 627
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 3 cut(s) 309, 556, 624
BstMCI CGRYCG 1 cut(s) 806
BstMWI GCNNNNNNNGC 3 cut(s) 74, 272, 701
BstNI CCWGG 3 cut(s) 591, 597, 814
BstNSI RCATGY 2 cut(s) 458, 679
BstSCI CCNGG 4 cut(s) 589, 595, 812, 845
BstSFI CTRYAG 2 cut(s) 143, 394
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 3 cut(s) 357, 493, 529
BstX2I RGATCY 1 cut(s) 624
BstYI RGATCY 1 cut(s) 624
BstZI CGGCCG 1 cut(s) 803
BsuRI GGCC 4 cut(s) 212, 638, 805, 839
BtsCI GGATG 1 cut(s) 472
BtsI GCAGTG 2 cut(s) 165, 822
BtsIMutI CAGTG 5 cut(s) 68, 157, 165, 721, 822
Cac8I GCNNGC 2 cut(s) 157, 677
CfoI GCGC 2 cut(s) 516, 675
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 2 cut(s) 593, 816
Csp6I GTAC 1 cut(s) 772
CviAII CATG 6 cut(s) 121, 455, 563, 619, 670, 676
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 3 cut(s) 337, 358, 367
DpnI GATC 3 cut(s) 311, 558, 626
DpnII GATC 3 cut(s) 309, 556, 624
EaeI YGGCCR 1 cut(s) 803
EagI CGGCCG 1 cut(s) 803
Eam1104I CTCTTC 2 cut(s) 338, 396
EarI CTCTTC 2 cut(s) 338, 396
EclXI CGGCCG 1 cut(s) 803
Eco130I CCWWGG 4 cut(s) 108, 240, 633, 754
Eco147I AGGCCT 1 cut(s) 839
Eco47I GGWCC 2 cut(s) 593, 816
Eco52I CGGCCG 1 cut(s) 803
EcoO109I RGGNCCY 1 cut(s) 816
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 3 cut(s) 589, 595, 812
EcoT14I CCWWGG 4 cut(s) 108, 240, 633, 754
ErhI CCWWGG 4 cut(s) 108, 240, 633, 754
FaeI CATG 6 cut(s) 124, 458, 566, 622, 673, 679
FaqI GGGAC 1 cut(s) 153
FatI CATG 6 cut(s) 120, 454, 562, 618, 669, 675
FbaI TGATCA 2 cut(s) 309, 556
Fnu4HI GCNGC 2 cut(s) 146, 803
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 2 cut(s) 146, 803
FspAI RTGCGCAY 1 cut(s) 674
FspBI CTAG 2 cut(s) 90, 779
FspI TGCGCA 2 cut(s) 515, 674
GlaI GCGC 2 cut(s) 515, 674
GluI GCNGC 2 cut(s) 146, 803
HaeIII GGCC 4 cut(s) 212, 638, 805, 839
HapII CCGG 3 cut(s) 425, 612, 847
HhaI GCGC 2 cut(s) 516, 675
Hin1II CATG 6 cut(s) 124, 458, 566, 622, 673, 679
Hin6I GCGC 2 cut(s) 514, 673
HinP1I GCGC 2 cut(s) 514, 673
HinfI GANTC 4 cut(s) 80, 365, 410, 690
HpaII CCGG 3 cut(s) 425, 612, 847
HphI GGTGA 3 cut(s) 313, 539, 770
Hpy166II GTNNAC 1 cut(s) 774
Hpy188I TCNGA 6 cut(s) 11, 27, 85, 340, 361, 370
Hpy188III TCNNGA 3 cut(s) 425, 766, 779
Hpy8I GTNNAC 1 cut(s) 774
HpyCH4III ACNGT 2 cut(s) 55, 716
HpyCH4V TGCA 4 cut(s) 35, 145, 493, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 272, 701
HpyF3I CTNAG 3 cut(s) 337, 358, 367
Hsp92II CATG 6 cut(s) 124, 458, 566, 622, 673, 679
HspAI GCGC 2 cut(s) 514, 673
Kpn2I TCCGGA 1 cut(s) 424
Ksp22I TGATCA 2 cut(s) 309, 556
Kzo9I GATC 3 cut(s) 309, 556, 624
LmnI GCTCC 2 cut(s) 302, 700
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 3 cut(s) 327, 501, 624
MaeI CTAG 2 cut(s) 90, 779
MaeIII GTNAC 1 cut(s) 601
MalI GATC 3 cut(s) 311, 558, 626
MboI GATC 3 cut(s) 309, 556, 624
MboII GAAGA 6 cut(s) 325, 362, 413, 493, 534, 575
MflI RGATCY 1 cut(s) 624
MluCI AATT 2 cut(s) 21, 748
MlyI GAGTC 3 cut(s) 74, 359, 699
MnlI CCTC 9 cut(s) 172, 199, 202, 264, 408, 432, 457, 637, 646
MroI TCCGGA 1 cut(s) 424
MseI TTAA 3 cut(s) 48, 291, 726
MslI CAYNNNNRTG 5 cut(s) 125, 170, 552, 674, 827
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 3 cut(s) 425, 612, 847
MspR9I CCNGG 4 cut(s) 591, 597, 814, 847
Mva1269I GAATGC 2 cut(s) 804, 814
MvaI CCWGG 3 cut(s) 591, 597, 814
MwoI GCNNNNNNNGC 3 cut(s) 74, 272, 701
NciI CCSGG 1 cut(s) 847
NdeII GATC 3 cut(s) 309, 556, 624
NlaIII CATG 6 cut(s) 124, 458, 566, 622, 673, 679
NlaIV GGNNCC 1 cut(s) 419
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 1 cut(s) 601
NsbI TGCGCA 2 cut(s) 515, 674
NspI RCATGY 2 cut(s) 458, 679
NspV TTCGAA 1 cut(s) 19
OliI CACNNNNGTG 2 cut(s) 170, 827
PaeI GCATGC 1 cut(s) 679
PceI AGGCCT 1 cut(s) 839
PciI ACATGT 1 cut(s) 454
PctI GAATGC 2 cut(s) 804, 814
PfeI GAWTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 2 cut(s) 147, 804
PleI GAGTC 3 cut(s) 74, 359, 698
PpsI GAGTC 3 cut(s) 74, 359, 698
PpuMI RGGWCCY 1 cut(s) 816
PscI ACATGT 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 816
Psp6I CCWGG 3 cut(s) 589, 595, 812
PspGI CCWGG 3 cut(s) 589, 595, 812
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 593, 816
PspPPI RGGWCCY 1 cut(s) 816
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 1 cut(s) 624
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 5 cut(s) 125, 170, 552, 674, 827
SaqAI TTAA 3 cut(s) 48, 291, 726
SatI GCNGC 2 cut(s) 146, 803
Sau3AI GATC 3 cut(s) 309, 556, 624
Sau96I GGNCC 2 cut(s) 593, 816
SchI GAGTC 3 cut(s) 74, 359, 699
ScrFI CCNGG 4 cut(s) 591, 597, 814, 847
SetI ASST 8 cut(s) 161, 307, 419, 529, 595, 648, 657, 821
SfaNI GCATC 3 cut(s) 327, 501, 624
SfcI CTRYAG 2 cut(s) 143, 394
SfuI TTCGAA 1 cut(s) 19
SinI GGWCC 2 cut(s) 593, 816
SmiMI CAYNNNNRTG 5 cut(s) 125, 170, 552, 674, 827
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 679
Sse9I AATT 2 cut(s) 21, 748
SseBI AGGCCT 1 cut(s) 839
SsiI CCGC 1 cut(s) 802
SspI AATATT 1 cut(s) 538
SspMI CTAG 2 cut(s) 90, 779
StuI AGGCCT 1 cut(s) 839
StyD4I CCNGG 4 cut(s) 589, 595, 812, 845
StyI CCWWGG 4 cut(s) 108, 240, 633, 754
TaaI ACNGT 2 cut(s) 55, 716
TaqI TCGA 3 cut(s) 19, 413, 462
TasI AATT 2 cut(s) 21, 748
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 805
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 3 cut(s) 48, 291, 726
Tru9I TTAA 3 cut(s) 48, 291, 726
TscAI CASTG 5 cut(s) 75, 157, 172, 721, 829
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 3 cut(s) 248, 396, 658
TspRI CASTG 5 cut(s) 75, 157, 172, 721, 829
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 2 cut(s) 593, 816
XapI RAATTY 1 cut(s) 21
XbaI TCTAGA 1 cut(s) 778
XceI RCATGY 2 cut(s) 458, 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XspI CTAG 2 cut(s) 90, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.