Rorug03G0033500
WRKY Family

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000471
Physical Location & Seq
Forward (+)
2672764 .. 2681539
8776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0033500.1

Sequence Viewer

Length: 5061 bp
ATGGCTTCCTCTTCTGTTTCTTCTTCTGTCATTCCCCCAAAAGAAAAGTATGATGTTTTTCTCAGTTTCAGAGGCGCGGATACTCGCCAGACTTTTACCAGCCATCTTTTTGCTGCTTTGATACGGTGGAAAGTGAAAACCTTCATTGATTACGGACTGGAGAGAGGAGATGAAATTGGACCCGCCCTTCTAAAAGCAATAAAAGAATCAAGGATTTCCGTGATCATTTTCTCGAAAGACTATGCTTCTTCCGCATGGTGCTTGGAAGAACTTGCTTGTATACTGGAATGCATGGAAAAACATGGACAATATGTTATACCTATTTTTTACGAGATAGAACCATCACAAGTGCGCTACCAGACCGGAAGTTATGAGACTGCATTTGCTCAACATGAACAATGGTTGAAGGACCACAAGACGGACAAGGTGGCCAAGTGGAAGGAAGCTTTAGTAAAAGCAGCTGGCCTATCTGGGTCAACTTCAAAAAAATTTCGGGATGATTCCGCTTTAGTTGAGCAAGTCGTCAAAGATGTGTTGGCTAAATTGAATCGTGAATCATCAAGCGATTTAACTGGTTTGATTGGAGCTGAAGACCGCATTAAGAAAATTTTACTGGAATTAGACATCATCCCAGAAGATGTTCGTGTTCGCTCTGTAGTTATTTGGGGTATGGGTGGTATCGGCAAGACCAGGCTTGCTGATGCTGTATATCATGGACTCTCTTCTCAATTCGACGCTCGCTGTTTTCTTGCAAATGTTAGGGAACGATCAGGTGCCGTTGACAAAGAGAGGCTCCGCCGTACAAAAGTCCTTGTTGTTCTTGACGACGTGAACCATTCAAGCCAATTAACATTTTTAGTTGGAGAAGTTCCATTTGGCCCAGGAAGTAGAATAATTATAACAACTCGAGATATGCAACCAGTTAGGAAGACTCTACTAGTGAAGGAAGTAGCTGGCCATGATGTTAAGATATACAAGGCGGAGGAATTAAATGATGTTGAGTCTCTTCAACTGTTCCATTCAAATGCTCGCACACATTGTACAGAAAATCCCGAACTTTTAGCGCAGGTGGCAGATCATGCTGCAGGAGTTCCATTAGCCCTTAAAATTTTGAGTTCATTATTCCTTCACTGCGAGAGCAAAGAAGAACAGGAACTGTTGTGGGAGAAATTGAAAAAGTTTCCTGATGATGACCTTCAAAATGTGTACAGAGCAAGTTTTGATGGATTAAAAGGAAATGAGAAGGAGATATTCCTTGATATAGCATGTTTTCACAAAAAGGAGAAGCTCTGTGATGCAAAAAGAATGTTAGCTGCCTGTGGTTTATTTCCGGATATTGGAATTAAAATTCTCACTGATATGTCTCTCATATCAATCAAAGACAACTGCATATGGATGCACGATGTGATCCAAGAAATGGGTTGGGAGATAGTCCGGGAAGAATGTACTGAAGAGCCAGGAAAACGCAGTAGGTTGCACAATGGTGAGGACGTCTGTCATGTATTGAAAAGGAATATGGGAACTGCCAAAGTACAAAGCATTTCCAGCAAGAATTGTGTTAACACACTAATGTTGGATCCTCAAGCTTTCACAGGGATGCATAACTTAAGATTCCTTATGCTAAGATTTATACGTCTTGATGACAAGGCAAATCTAGAGTATCTGCCTGATGCCCTTCAATATCTCTTTTGGCATAGTTACCCCTTGAAATCTTTTCCATCAAAGTTTTCTCCAAACAATCTTGTTGAGCTTCATATGCCCTGGAGCAAACTCAAGAGACTTTGGAATAAAGGCCAGAATCCTAAGAACTTGAAAAGGATCGATCTTAGTCACTCCATGGACCTGGCCGATGTTGGAGAGCTGTCAAATAGTGTAAAAATTGAGAGTATAAATCTTGGAGGCTGTAAAAGTTTGGTTCAAGTTCCGGATTTGTCAAAGTGTGTATACATTAAGAGTATAAATCTCCTAGGCTGCGCAAGTTTGGTTCGAGTTCCAGACCTGTCAAAGAGTGTAAACATTGAGAGTATAAATCTTCAATACTGTGATGGTTTGGTTCAAGTTCCTTCATACTTTCAAAACTTCACAAGGCTTACTCATTTGAATTTGGGAAATTGTTGGAAGAATAGAGTTCTACAGAAGATACCAAGCAATATGGAATTCTTACATTTGGGTCATACTGCTATAGAAGAATTGCCTCCATCAATTTGGTCTCTTGAAAAACTTCTTATATTGAATCTTCGTGACTGTTCCGCCATTAAGAATTTTCCAAGCAGTCCATGGAAGATGAAATCCCTCACTCATCTTTTGTTGAGCGGGACAAACAAAGAAACAGTGCCCTCATCGTCATTCATGTGTATGACGGGGATCATTTCACTTGAGCTGAGAGATTGTCAACACCTCGTCAGTCTCCCAACCGACATTTGTAAGTTGAAATCTCTTGAGAGACTTGATCTCTTTCGTTGCCGTCGTTTCACAAAATTTCCGGAAATTGCGGAGCCTATGGAACATCTGCAGTATCTTAATTTAAGTGGGACGAAAATTGAAGAGCTGCCGTCATCGGTTGGGAATCTTGTTGGGCTTAAGACATTAGATTTATCTGACTGCACAAGCCTTGAATTACTCCCAAACAACTTCTACAATTTAAACCTTCTCGAGTGGTTCTCACTTCGCAACTGTGTTCAGCTAAAAAAATTGCCTCTCTCGTTCATTTTGTGCTCTTTGATAAATCTAAACCTCAGTGGCTGCAAATTGTTAGAAGAAATTCCTGATTGCATTACTAGCTTTCCCGCATTGCAAGTGTTAGATCTAAGTCACACCATGATTGAGACCATACCTCCCAGCATCAAACAAGTTTCAAGGCTCAAGCATCTGTTCCTAAATGGTTGCGAGTGGCTTCAATCTTTGCCAGTGCTCCCATGTCTTCTGAAAAAGTTGGTTGCAGATGAGTGTACGAGACTTAAGACAGTATCAGTTTCAGTGATTGCACAGACGGAAGGTTTGGATCAAATTGTTTCTGGGAAAAAGAGGTCCGAGAGACATACATATTATGGTTGCGATAATTTGGATAAAAATGCAAGGAGCAACATAATGGATGATGCACACTTCAGAATTATGCGAATGGCAACTGCTTGTAATCTTAAACGTTTATCATCTGGTAAAGTAGAGTTAATGGGTCCGGGAAATGAAATTCCAAAATGGTTTAGCTGTCAAACGGAGGGATCTTCAATGAATATTAAGCTTCCTCTGCATTGGTCTGATGATTCAAACTTCTTGGGTATTGCTCTCTGCTCTGTTTCTGCAATCCATGCATATTCTCTCTCTCTCGGGTGTCAATGTGAGATGATTCTCAAAACCAACGGCGGTGAAACCCATAGTGTCAATTTGGGAACTACAGGGGACTTCCGGAACTTCCAGAATACGGACCCGATGGCTGAACCAGATCAAGTTTTGGTGTGGTGTGATACAGTCCCTGTAATTTCAGATGAAGCAAAATGGTCTATGGAAGCCTCTTTTCACTTCTATACAGTACTCGATGGTAAACGGTCAAATAACGTGAAAAGGTGTGGGGTCTGCTTTCTGTATGCCCAAAGCCAAGATGATGATGCTCCGAAATTTGAAGTCATTCGAACAGTTCCCTCGTTATCATTCATGTGTATGACTGGGACCATTTCACTTGACCTGAGTTATTGTGATCGCCTTGTCAGTCTCCCAGCCGACATTTGTAAGTTGAAATATCTTGAGAGACTTAATCTCTCTGGTTGCCGTAGATTCACAAAATTTCCGGATATTGTGGAGCCTATGGAACATCTGCAGTATCTTAATTTGAGCGGGACGAACATTAAAAAGCTACCCTCAACGATTGGGAATCTTGTTGGGCTTAAGACGTTAATTTTAACTTTCTGCAAAAGCCTTGAATTACTCCCAGATAGCTTCTACAATTTAAACCTTCTCGAGTGGTTCTCACTTGAGGGCTGTGTCAAGCTGGAAAAATTGCCACTCTCGTTCAACAATTTAAACCTTCTCGAGTGGTTCTCACTTGAGAGCTGTGTCGAGCTAGAAAAAATGCCACTCTCGTTCAGTTTGTGCTCTTTGATAAATCTGAGCTTCAGAGGCTGCATATTGTTAGAAGAAATTCCCGATTGCTTCAATAGCTTTCCCGCATTGCAAGTCTTGGATCTCAGTGAAACCATGATTGAGACCATACCTCCCAGCATCAAACAAGTTTTTGGGCTCAAGTCTCTGCTACTAGATCAGTGCAAGCGGCTTCAATCTTTGCCAGTGCTCCCATGTCTGCTAGAAGAGTTCTATGCAAGAGATTGCTGGAGACTTAAGACAGTGCCAGTTTCAGTGACTGCACAGGCACAAGGTTTAGATCAAGTACTGTCTGGGTATAGGTACGAGACCCATTCATTTGTTGGTTGCGTTAATTTGGATAACAATTCAAGGAGTAACATAATGGATGATGCACACATCAGAATTATGCGAATAGCAACTGCTTGTAATCTTAAACGTTTATCATATGGTGATGTAGAGGTAATGTGTCCGGGAAATGAAATTCCAAAATGGTTTAGCTGTCAAACAGAGGGATCTTCAATGAATATTAAGCTTCCTCTGCATTGGTCTGATGATTCAAAATTCTTGGGTATTGCTCTCTGCTCTGTTTCTGCAATCCATGAAGATCTCCATCATCCCGAGTATCAATGTGAGATGATTCTCAAAACCAACAATGGTGAAACCCATAGTGTCAATTTGGGAAGTGAAGAGTGCTTCGGGACTATGCTCCTGTGGGGTCAAGAAGATGACGTGTCCGTGTGGTATAATAAAGTCCCTGCAATTTCAGATGAAGCAAAATGGTCTACGGAAGCCTCTTTTGACTTCTATAGAGACGACGATGGTAGACGGTGGAATAACGTGAAAAGGTGTGGGGTCTGCTTTCTGTATGCCCAAGGCCAAGATGATGATGCTCTGAAATTTGAAGTCATTCGTCCACAGCAAGTTACAACACAACAAGTGACAACACAACAAGTTACAGCAAGTGTTGCAACTTGCTTACTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

1686

Amino Acids

190.46

Weight (kDa)

6.16

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 192 3.9e-55 TIR domain
TIR_2 PF13676 19 - 114 4e-12 TIR domain
WHD_ROQ1 PF23282 413 - 481 1.6e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 689 - 818 1.4e-08 Leucine-rich repeat region
LRR_13 PF23286 810 - 990 2.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 835 - 895 1.4e-09 Leucine-rich repeat region
C-JID PF20160 1059 - 1197 2.3e-14 C-JID domain
LRR_13 PF23286 1244 - 1428 2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1267 - 1349 6e-10 Leucine-rich repeat region
C-JID PF20160 1514 - 1649 1.6e-14 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000471)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18190 AT2G18193
fragaria_vesca FvH4_1g12130 FvH4_1g12690 FvH4_1g12700 FvH4_1g12712 FvH4_1g12716 FvH4_1g12731
malus_domestica MD02G1141300.v1.1 MD02G1141400.v1.1 MD02G1141600.v1.1 MD02G1141800.v1.1 MD02G1142200.v1.1 MD15G1254200.v1.1
prunus_persica Prupe.7G159500_v2.0.a1 Prupe.7G159600_v2.0.a1 Prupe.7G165500_v2.0.a1
pyrus_communis pycom02g11130 pycom02g11150 pycom02g11160 pycom02g11180 pycom15g22350
rosa_chinensis RchiOBHm_Chr2g0099791 RchiOBHm_Chr2g0099801 RchiOBHm_Chr2g0100651 RchiOBHm_Chr2g0100681 RchiOBHm_Chr2g0100691 RchiOBHm_Chr2g0100701 RchiOBHm_Chr2g0100721 RchiOBHm_Chr2g0100801 RchiOBHm_Chr3g0459141
rosa_laevigata RLG00000016914 RLG00000016915 RLG00000016997 RLG00000016998 RLG00000017000 RLG00000017002 RLG00000017012 RLG00000025114
rosa_multiflora Rmu_co8416275.1_g000001 Rmu_sc0001187.1_g000004 Rmu_sc0001497.1_g000006 Rmu_sc0001659.1_g000004 Rmu_sc0001659.1_g000006 Rmu_sc0001659.1_g000008 Rmu_sc0001659.1_g000010 Rmu_sc0008864.1_g000008 Rmu_sc0028022.1_g000001
rosa_roxburghii Rroxscaffold_2G00141850 Rroxscaffold_2G00141930 Rroxscaffold_2G00141940 Rroxscaffold_2G00141950 Rroxscaffold_2G00141960 Rroxscaffold_2G00141970
rosa_rugosa Rorug02G0091800 Rorug02G0091800 Rorug02G0091800 Rorug02G0091900 Rorug02G0092900 Rorug03G0033500
rosa_samantha Rh2BG135600 Rh2BG143900 Rh2BG144000 Rh2BG144200 Rh2BG144300 Rh2BG144400 Rh2BG144500 Rh2BG145500 Rh2CG136900 Rh2CG137000 Rh2CG144800 Rh2CG145000 Rh2CG145100 Rh2CG145200 Rh2CG145500 Rh2CG146400 Rh2DG137400 Rh2DG137500 Rh2DG144300 Rh2DG144500 Rh2DG144600 Rh2DG144700 Rh2DG145000 Rh2DG145900 Rh3BG094700 Rh3DG095300
rosa_wichuraiana Rw2G010920 Rw2G010950 Rw2G010990 Rw3G007860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 1 cut(s) 1057
AatII GACGTC 1 cut(s) 1494
Acc16I TGCGCA 1 cut(s) 1975
Acc36I ACCTGC 1 cut(s) 1057
AccB1I GGYRCC 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 1417
AccBSI CCGCTC 2 cut(s) 2313, 3828
AccI GTMKAC 4 cut(s) 280, 1944, 4856, 4897
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
AclI AACGTT 2 cut(s) 3142, 4510
AclWI GGATC 9 cut(s) 1402, 1571, 1584, 1826, 2372, 3009, 3226, 4182, 4594
AcoI YGGCCR 3 cut(s) 429, 955, 1845
AcuI CTGAAG 4 cut(s) 609, 1470, 3088, 4090
AcyI GRCGYC 1 cut(s) 1491
AdeI CACNNNGTG 1 cut(s) 1405
AfaI GTAC 9 cut(s) 802, 1042, 1208, 1447, 1533, 2950, 3528, 4380, 4397
AfiI CCNNNNNNNGG 4 cut(s) 418, 1337, 1417, 3418
AflII CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
AflIII ACRYGT 1 cut(s) 4803
AhlI ACTAGT 1 cut(s) 937
AjiI CACGTC 2 cut(s) 829, 4804
AjnI CCWGG 5 cut(s) 689, 880, 1456, 1760, 1842
AjuI GAANNNNNNNTTGG 2 cut(s) 858, 890
AleI CACNNNNGTG 1 cut(s) 1482
Alw21I GWGCWC 4 cut(s) 2717, 2913, 4088, 4284
AlwI GGATC 9 cut(s) 1402, 1571, 1584, 1826, 2372, 3009, 3226, 4182, 4594
AlwNI CAGNNNCTG 5 cut(s) 1156, 2742, 3470, 4113, 4352
Ama87I CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
Aor13HI TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
AoxI GGCC 7 cut(s) 429, 463, 877, 955, 1792, 1845, 4948
ApeKI GCWGC 8 cut(s) 113, 458, 1082, 1313, 1971, 2548, 2742, 4113
ArsI GACNNNNNNTTYG 2 cut(s) 2980, 3012
Asp700I GAANNNNTTC 7 cut(s) 140, 639, 2220, 2760, 3621, 4131, 4980
AspA2I CCTAGG 1 cut(s) 1966
AspLEI GCGC 4 cut(s) 77, 354, 1066, 1976
AspS9I GGNCC 8 cut(s) 179, 409, 878, 1840, 3027, 3173, 3421, 3663
AsuC2I CCSGG 3 cut(s) 1436, 3177, 4545
AsuHPI GGTGA 4 cut(s) 1496, 3374, 4535, 4742
AsuII TTCGAA 1 cut(s) 3625
AvaI CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
AvaII GGWCC 7 cut(s) 179, 409, 1840, 3027, 3173, 3421, 3663
AvrII CCTAGG 1 cut(s) 1966
BaeGI GKGCMC 1 cut(s) 2337
BalI TGGCCA 2 cut(s) 431, 957
BamHI GGATCC 1 cut(s) 1576
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 1 cut(s) 4233
BbsI GAAGAC 3 cut(s) 597, 935, 2912
Bbv12I GWGCWC 4 cut(s) 2717, 2913, 4088, 4284
BbvI GCAGC 8 cut(s) 100, 470, 1069, 1300, 1958, 2535, 2729, 4100
BceAI ACGGC 6 cut(s) 761, 783, 2448, 2536, 3373, 3747
BciT130I CCWGG 5 cut(s) 691, 882, 1458, 1762, 1844
BciVI GTATCC 1 cut(s) 73
BclI TGATCA 1 cut(s) 222
BcnI CCSGG 3 cut(s) 1436, 3177, 4545
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 7 cut(s) 938, 1655, 1967, 2778, 4055, 4247, 4295
BfmI CTRYAG 8 cut(s) 654, 1083, 2132, 2181, 2510, 3390, 3809, 4879
BfrI CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
BfuAI ACCTGC 1 cut(s) 1057
BfuI GTATCC 1 cut(s) 73
BglII AGATCT 2 cut(s) 2803, 4678
BisI GCNGC 9 cut(s) 114, 459, 1083, 1314, 1972, 2549, 2743, 4114, 4262
BlnI CCTAGG 1 cut(s) 1966
BlsI GCNGC 9 cut(s) 115, 460, 1084, 1315, 1973, 2550, 2744, 4115, 4263
BmcAI AGTACT 2 cut(s) 3528, 4380
Bme1390I CCNGG 8 cut(s) 691, 882, 1436, 1458, 1762, 1844, 3177, 4545
Bme18I GGWCC 7 cut(s) 179, 409, 1840, 3027, 3173, 3421, 3663
BmeT110I CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
BmgBI CACGTC 2 cut(s) 829, 4804
BmgT120I GGNCC 8 cut(s) 179, 409, 878, 1840, 3027, 3173, 3421, 3663
BmiI GGNNCC 9 cut(s) 181, 775, 794, 1578, 2496, 3174, 3423, 3664, 3795
BmrFI CCNGG 8 cut(s) 691, 882, 1436, 1458, 1762, 1844, 3177, 4545
BmrI ACTGGG 1 cut(s) 3669
BmuI ACTGGG 1 cut(s) 3669
BoxI GACNNNNGTC 1 cut(s) 1494
BpiI GAAGAC 3 cut(s) 597, 935, 2912
BplI GAGNNNNNCTC 6 cut(s) 2645, 2677, 3944, 3976, 4016, 4048
BpmI CTGGAG 3 cut(s) 179, 1783, 4342
Bpu14I TTCGAA 1 cut(s) 3625
BpuEI CTTGAG 9 cut(s) 1566, 1757, 2396, 2459, 2846, 3758, 3986, 4058, 4217
BpuMI CCSGG 3 cut(s) 1436, 3177, 4545
Bsa29I ATCGAT 1 cut(s) 1821
BsaBI GATNNNNATC 1 cut(s) 4683
BsaHI GRCGYC 1 cut(s) 1491
BsaI GGTCTC 4 cut(s) 2214, 2819, 4190, 4394
BsaJI CCNNGG 6 cut(s) 880, 1760, 1836, 1966, 2276, 4945
BsaWI WCCGGW 6 cut(s) 362, 1330, 1924, 2482, 3402, 3781
BsaXI ACNNNNNCTCC 4 cut(s) 2818, 2848, 4189, 4219
Bsc4I CCNNNNNNNGG 4 cut(s) 418, 1337, 1417, 3418
Bse1I ACTGG 9 cut(s) 162, 288, 577, 618, 920, 2906, 3664, 4277, 4340
Bse3DI GCAATG 2 cut(s) 2789, 4160
Bse8I GATNNNNATC 1 cut(s) 4683
BseAI TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
BseBI CCWGG 5 cut(s) 691, 882, 1458, 1762, 1844
BseCI ATCGAT 1 cut(s) 1821
BseDI CCNNGG 6 cut(s) 880, 1760, 1836, 1966, 2276, 4945
BseGI GGATG 7 cut(s) 502, 627, 1401, 1602, 3097, 4465, 4687
BseJI GATNNNNATC 1 cut(s) 4683
BseLI CCNNNNNNNGG 4 cut(s) 418, 1337, 1417, 3418
BseMI GCAATG 2 cut(s) 2789, 4160
BseMII CTCAG 6 cut(s) 76, 2372, 2749, 3671, 4091, 4192
BseNI ACTGG 9 cut(s) 162, 288, 577, 618, 920, 2906, 3664, 4277, 4340
BseRI GAGGAG 1 cut(s) 180
BseSI GKGCMC 1 cut(s) 2337
BseXI GCAGC 8 cut(s) 100, 470, 1069, 1300, 1958, 2535, 2729, 4100
BseYI CCCAGC 3 cut(s) 2837, 3709, 4208
BsgI GTGCAG 2 cut(s) 2587, 4338
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 7 cut(s) 431, 465, 879, 957, 1794, 1847, 4950
BshNI GGYRCC 1 cut(s) 773
BshVI ATCGAT 1 cut(s) 1821
BsiHKAI GWGCWC 4 cut(s) 2717, 2913, 4088, 4284
BsiHKCI CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
BsiSI CCGG 9 cut(s) 363, 1331, 1435, 1925, 2483, 3176, 3403, 3782, 4544
BslFI GGGAC 8 cut(s) 2329, 2545, 3410, 3452, 3676, 3844, 4786, 4811
BslI CCNNNNNNNGG 4 cut(s) 418, 1337, 1417, 3418
BsmBI CGTCTC 1 cut(s) 4878
BsmFI GGGAC 8 cut(s) 2329, 2545, 3410, 3452, 3676, 3844, 4786, 4811
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 7 cut(s) 431, 465, 879, 957, 1794, 1847, 4950
Bso31I GGTCTC 4 cut(s) 2214, 2819, 4190, 4394
BsoBI CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
Bsp119I TTCGAA 1 cut(s) 3625
Bsp1286I GDGCHC 6 cut(s) 2337, 2717, 2913, 4088, 4233, 4284
Bsp13I TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
Bsp1407I TGTACA 2 cut(s) 1040, 1206
Bsp19I CCATGG 2 cut(s) 1836, 2276
BspANI GGCC 7 cut(s) 431, 465, 879, 957, 1794, 1847, 4950
BspCNI CTCAG 6 cut(s) 75, 2373, 2748, 3672, 4092, 4191
BspDI ATCGAT 1 cut(s) 1821
BspEI TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 9 cut(s) 181, 775, 794, 1578, 2496, 3174, 3423, 3664, 3795
BspMAI CTGCAG 3 cut(s) 1087, 2514, 3813
BspMI ACCTGC 1 cut(s) 1057
BspPI GGATC 9 cut(s) 1402, 1571, 1584, 1826, 2372, 3009, 3226, 4182, 4594
BspQI GCTCTTC 2 cut(s) 1446, 2538
BspT104I TTCGAA 1 cut(s) 3625
BspT107I GGYRCC 1 cut(s) 773
BspTI CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
BspTNI GGTCTC 4 cut(s) 2214, 2819, 4190, 4394
BsrBI CCGCTC 2 cut(s) 2313, 3828
BsrDI GCAATG 2 cut(s) 2789, 4160
BsrGI TGTACA 2 cut(s) 1040, 1206
BsrI ACTGG 9 cut(s) 162, 288, 577, 618, 920, 2906, 3664, 4277, 4340
BssECI CCNNGG 6 cut(s) 880, 1760, 1836, 1966, 2276, 4945
BssNAI GTATAC 2 cut(s) 281, 1945
BssNI GRCGYC 1 cut(s) 1491
BssT1I CCWWGG 4 cut(s) 1836, 1966, 2276, 4945
Bst1107I GTATAC 2 cut(s) 281, 1945
Bst2UI CCWGG 5 cut(s) 691, 882, 1458, 1762, 1844
Bst6I CTCTTC 7 cut(s) 16, 727, 1011, 1446, 2538, 4293, 4755
BstACI GRCGYC 1 cut(s) 1491
BstAFI CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
BstAPI GCANNNNNTGC 3 cut(s) 1079, 3305, 5039
BstAUI TGTACA 2 cut(s) 1040, 1206
BstBI TTCGAA 1 cut(s) 3625
BstC8I GCNNGC 6 cut(s) 463, 696, 739, 955, 1030, 4259
BstDSI CCRYGG 2 cut(s) 1836, 2276
BstF5I GGATG 7 cut(s) 502, 627, 1401, 1602, 3097, 4465, 4687
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 4 cut(s) 77, 354, 1066, 1976
BstNI CCWGG 5 cut(s) 691, 882, 1458, 1762, 1844
BstNSI RCATGY 1 cut(s) 1269
BstPAI GACNNNNGTC 1 cut(s) 1494
BstSCI CCNGG 8 cut(s) 689, 880, 1434, 1456, 1760, 1842, 3175, 4543
BstSFI CTRYAG 8 cut(s) 654, 1083, 2132, 2181, 2510, 3390, 3809, 4879
BstSLI GKGCMC 1 cut(s) 2337
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 8 cut(s) 100, 470, 1069, 1300, 1958, 2535, 2729, 4100
BstV2I GAAGAC 3 cut(s) 597, 935, 2912
BstX2I RGATCY 6 cut(s) 1576, 2803, 3218, 4174, 4586, 4678
BstXI CCANNNNNNTGG 2 cut(s) 1843, 2205
BstYI RGATCY 6 cut(s) 1576, 2803, 3218, 4174, 4586, 4678
BstZ17I GTATAC 2 cut(s) 281, 1945
Bsu15I ATCGAT 1 cut(s) 1821
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 7 cut(s) 431, 465, 879, 957, 1794, 1847, 4950
BsuTUI ATCGAT 1 cut(s) 1821
BtgI CCRYGG 2 cut(s) 1836, 2276
BtrI CACGTC 2 cut(s) 829, 4804
BtsCI GGATG 7 cut(s) 502, 627, 1401, 1602, 3097, 4465, 4687
BtsI GCAGTG 1 cut(s) 1129
BveI ACCTGC 1 cut(s) 1057
Cac8I GCNNGC 6 cut(s) 463, 696, 739, 955, 1030, 4259
CaiI CAGNNNCTG 5 cut(s) 1156, 2742, 3470, 4113, 4352
CfoI GCGC 4 cut(s) 77, 354, 1066, 1976
Cfr13I GGNCC 8 cut(s) 179, 409, 878, 1840, 3027, 3173, 3421, 3663
ClaI ATCGAT 1 cut(s) 1821
CseI GACGC 1 cut(s) 743
Csp6I GTAC 9 cut(s) 801, 1041, 1207, 1446, 1532, 2949, 3527, 4379, 4396
CviQI GTAC 9 cut(s) 801, 1041, 1207, 1446, 1532, 2949, 3527, 4379, 4396
DraI TTTAAA 3 cut(s) 2643, 3942, 4014
DraIII CACNNNGTG 1 cut(s) 1405
EaeI YGGCCR 3 cut(s) 429, 955, 1845
Eam1104I CTCTTC 7 cut(s) 16, 727, 1011, 1446, 2538, 4293, 4755
EarI CTCTTC 7 cut(s) 16, 727, 1011, 1446, 2538, 4293, 4755
EciI GGCGGA 3 cut(s) 785, 995, 2239
Eco130I CCWWGG 4 cut(s) 1836, 1966, 2276, 4945
Eco24I GRGCYC 1 cut(s) 4233
Eco31I GGTCTC 4 cut(s) 2214, 2819, 4190, 4394
Eco47I GGWCC 7 cut(s) 179, 409, 1840, 3027, 3173, 3421, 3663
Eco57I CTGAAG 4 cut(s) 609, 1470, 3088, 4090
Eco88I CYCGRG 6 cut(s) 906, 2651, 3323, 3950, 4022, 4691
EcoRI GAATTC 1 cut(s) 2156
EcoRII CCWGG 5 cut(s) 689, 880, 1456, 1760, 1842
EcoT14I CCWWGG 4 cut(s) 1836, 1966, 2276, 4945
EcoT22I ATGCAT 3 cut(s) 293, 1602, 3310
EcoT38I GRGCYC 1 cut(s) 4233
ErhI CCWWGG 4 cut(s) 1836, 1966, 2276, 4945
Esp3I CGTCTC 1 cut(s) 4878
FaqI GGGAC 8 cut(s) 2329, 2545, 3410, 3452, 3676, 3844, 4786, 4811
FauI CCCGC 5 cut(s) 190, 2306, 2794, 3821, 4165
FauNDI CATATG 3 cut(s) 1391, 1755, 4519
FbaI TGATCA 1 cut(s) 222
FblI GTMKAC 4 cut(s) 280, 1944, 4856, 4897
Fnu4HI GCNGC 9 cut(s) 114, 459, 1083, 1314, 1972, 2549, 2743, 4114, 4262
FokI GGATG 7 cut(s) 509, 614, 1408, 1609, 3104, 4472, 4674
FriOI GRGCYC 1 cut(s) 4233
Fsp4HI GCNGC 9 cut(s) 114, 459, 1083, 1314, 1972, 2549, 2743, 4114, 4262
FspBI CTAG 7 cut(s) 938, 1655, 1967, 2778, 4055, 4247, 4295
FspI TGCGCA 1 cut(s) 1975
GlaI GCGC 4 cut(s) 76, 353, 1065, 1975
GluI GCNGC 9 cut(s) 114, 459, 1083, 1314, 1972, 2549, 2743, 4114, 4262
GsaI CCCAGC 3 cut(s) 2841, 3713, 4212
GsuI CTGGAG 3 cut(s) 179, 1783, 4342
HaeIII GGCC 7 cut(s) 431, 465, 879, 957, 1794, 1847, 4950
HapII CCGG 9 cut(s) 363, 1331, 1435, 1925, 2483, 3176, 3403, 3782, 4544
HgaI GACGC 1 cut(s) 743
HhaI GCGC 4 cut(s) 77, 354, 1066, 1976
Hin1I GRCGYC 1 cut(s) 1491
Hin6I GCGC 4 cut(s) 75, 352, 1064, 1974
HinP1I GCGC 4 cut(s) 75, 352, 1064, 1974
HincII GTYRAC 4 cut(s) 477, 781, 1561, 2393
HindII GTYRAC 4 cut(s) 477, 781, 1561, 2393
HindIII AAGCTT 4 cut(s) 444, 1584, 3236, 4604
HpaI GTTAAC 1 cut(s) 1561
HpaII CCGG 9 cut(s) 363, 1331, 1435, 1925, 2483, 3176, 3403, 3782, 4544
HphI GGTGA 4 cut(s) 1496, 3374, 4535, 4742
Hpy99I CGWCG 4 cut(s) 737, 830, 2469, 4892
HpyCH4IV ACGT 9 cut(s) 828, 1491, 1633, 3142, 3552, 3884, 4510, 4803, 4911
HpySE526I ACGT 9 cut(s) 828, 1491, 1633, 3142, 3552, 3884, 4510, 4803, 4911
Hsp92I GRCGYC 1 cut(s) 1491
HspAI GCGC 4 cut(s) 75, 352, 1064, 1974
Kpn2I TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
Ksp22I TGATCA 1 cut(s) 222
KspAI GTTAAC 1 cut(s) 1561
LguI GCTCTTC 2 cut(s) 1446, 2538
Lsp1109I GCAGC 8 cut(s) 100, 470, 1069, 1300, 1958, 2535, 2729, 4100
MaeI CTAG 7 cut(s) 938, 1655, 1967, 2778, 4055, 4247, 4295
MaeII ACGT 9 cut(s) 828, 1491, 1633, 3142, 3552, 3884, 4510, 4803, 4911
MaeIII GTNAC 9 cut(s) 1697, 1829, 2240, 2810, 4348, 4448, 4996, 5011, 5026
MbiI CCGCTC 2 cut(s) 2313, 3828
MflI RGATCY 6 cut(s) 1576, 2803, 3218, 4174, 4586, 4678
MhlI GDGCHC 6 cut(s) 2337, 2717, 2913, 4088, 4233, 4284
MlsI TGGCCA 2 cut(s) 431, 957
MluNI TGGCCA 2 cut(s) 431, 957
MlyI GAGTC 3 cut(s) 711, 925, 1010
MmeI TCCRAC 4 cut(s) 841, 1554, 1834, 2096
Mox20I TGGCCA 2 cut(s) 431, 957
Mph1103I ATGCAT 3 cut(s) 293, 1602, 3310
MroI TCCGGA 5 cut(s) 1330, 1924, 2482, 3402, 3781
MroXI GAANNNNTTC 7 cut(s) 140, 639, 2220, 2760, 3621, 4131, 4980
MscI TGGCCA 2 cut(s) 431, 957
Msp20I TGGCCA 2 cut(s) 431, 957
MspA1I CMGCKG 1 cut(s) 461
MspCI CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
MspI CCGG 9 cut(s) 363, 1331, 1435, 1925, 2483, 3176, 3403, 3782, 4544
MspR9I CCNGG 8 cut(s) 691, 882, 1436, 1458, 1762, 1844, 3177, 4545
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 5 cut(s) 691, 882, 1458, 1762, 1844
MvnI CGCG 1 cut(s) 77
NciI CCSGG 3 cut(s) 1436, 3177, 4545
NcoI CCATGG 2 cut(s) 1836, 2276
NdeI CATATG 3 cut(s) 1391, 1755, 4519
NlaIV GGNNCC 9 cut(s) 181, 775, 794, 1578, 2496, 3174, 3423, 3664, 3795
NmuCI GTSAC 5 cut(s) 1829, 2240, 2810, 4348, 5011
NsbI TGCGCA 1 cut(s) 1975
NsiI ATGCAT 3 cut(s) 293, 1602, 3310
NspI RCATGY 1 cut(s) 1269
NspV TTCGAA 1 cut(s) 3625
OliI CACNNNNGTG 1 cut(s) 1482
PaeR7I CTCGAG 4 cut(s) 906, 2651, 3950, 4022
PaqCI CACCTGC 1 cut(s) 1057
PciSI GCTCTTC 2 cut(s) 1446, 2538
PcsI WCGNNNNNNNCGW 1 cut(s) 2464
PctI GAATGC 1 cut(s) 293
PdmI GAANNNNTTC 7 cut(s) 140, 639, 2220, 2760, 3621, 4131, 4980
PflMI CCANNNNNTGG 1 cut(s) 1417
PfoI TCCNGGA 3 cut(s) 1434, 3175, 4543
PkrI GCNGC 9 cut(s) 115, 460, 1084, 1315, 1973, 2550, 2744, 4115, 4263
PleI GAGTC 3 cut(s) 711, 925, 1009
PpsI GAGTC 3 cut(s) 711, 925, 1009
PshAI GACNNNNGTC 1 cut(s) 1494
PsiI TTATAA 1 cut(s) 899
Psp1406I AACGTT 2 cut(s) 3142, 4510
Psp6I CCWGG 5 cut(s) 689, 880, 1456, 1760, 1842
PspFI CCCAGC 3 cut(s) 2837, 3709, 4208
PspGI CCWGG 5 cut(s) 689, 880, 1456, 1760, 1842
PspN4I GGNNCC 9 cut(s) 181, 775, 794, 1578, 2496, 3174, 3423, 3664, 3795
PspPI GGNCC 8 cut(s) 179, 409, 878, 1840, 3027, 3173, 3421, 3663
PstI CTGCAG 3 cut(s) 1087, 2514, 3813
PstNI CAGNNNCTG 5 cut(s) 1156, 2742, 3470, 4113, 4352
PsuI RGATCY 6 cut(s) 1576, 2803, 3218, 4174, 4586, 4678
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 9 cut(s) 802, 1042, 1208, 1447, 1533, 2950, 3528, 4380, 4397
RsaNI GTAC 9 cut(s) 801, 1041, 1207, 1446, 1532, 2949, 3527, 4379, 4396
SapI GCTCTTC 2 cut(s) 1446, 2538
SatI GCNGC 9 cut(s) 114, 459, 1083, 1314, 1972, 2549, 2743, 4114, 4262
Sau96I GGNCC 8 cut(s) 179, 409, 878, 1840, 3027, 3173, 3421, 3663
ScaI AGTACT 2 cut(s) 3528, 4380
SchI GAGTC 3 cut(s) 711, 925, 1010
ScrFI CCNGG 8 cut(s) 691, 882, 1436, 1458, 1762, 1844, 3177, 4545
SduI GDGCHC 6 cut(s) 2337, 2717, 2913, 4088, 4233, 4284
SfcI CTRYAG 8 cut(s) 654, 1083, 2132, 2181, 2510, 3390, 3809, 4879
Sfr274I CTCGAG 4 cut(s) 906, 2651, 3950, 4022
SfuI TTCGAA 1 cut(s) 3625
SinI GGWCC 7 cut(s) 179, 409, 1840, 3027, 3173, 3421, 3663
SlaI CTCGAG 4 cut(s) 906, 2651, 3950, 4022
SpeI ACTAGT 1 cut(s) 937
SspI AATATT 2 cut(s) 3232, 4600
SspMI CTAG 7 cut(s) 938, 1655, 1967, 2778, 4055, 4247, 4295
StyD4I CCNGG 8 cut(s) 689, 880, 1434, 1456, 1760, 1842, 3175, 4543
StyI CCWWGG 4 cut(s) 1836, 1966, 2276, 4945
TaiI ACGT 9 cut(s) 831, 1494, 1636, 3145, 3555, 3887, 4513, 4806, 4914
TatI WGTACW 6 cut(s) 1040, 1206, 1445, 1531, 3526, 4378
TauI GCSGC 1 cut(s) 4264
TseFI GTSAC 5 cut(s) 1829, 2240, 2810, 4348, 5011
TseI GCWGC 8 cut(s) 113, 458, 1082, 1313, 1971, 2548, 2742, 4113
Tsp45I GTSAC 5 cut(s) 1829, 2240, 2810, 4348, 5011
TspGWI ACGGA 8 cut(s) 168, 208, 434, 3005, 3227, 3434, 4798, 4874
Van91I CCANNNNNTGG 1 cut(s) 1417
Vha464I CTTAAG 5 cut(s) 1606, 2579, 2957, 3878, 4328
VpaK11BI GGWCC 7 cut(s) 179, 409, 1840, 3027, 3173, 3421, 3663
XbaI TCTAGA 1 cut(s) 1654
XceI RCATGY 1 cut(s) 1269
XcmI CCANNNNNNNNNTGG 2 cut(s) 2274, 4412
XhoI CTCGAG 4 cut(s) 906, 2651, 3950, 4022
XmaJI CCTAGG 1 cut(s) 1966
XmiI GTMKAC 4 cut(s) 280, 1944, 4856, 4897
XmnI GAANNNNTTC 7 cut(s) 140, 639, 2220, 2760, 3621, 4131, 4980
XspI CTAG 7 cut(s) 938, 1655, 1967, 2778, 4055, 4247, 4295
ZraI GACGTC 1 cut(s) 1492
ZrmI AGTACT 2 cut(s) 3528, 4380
Zsp2I ATGCAT 3 cut(s) 293, 1602, 3310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.