Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000471

Physical Location & Seq

Genomic Coordinates Forward (+)

11743826 .. 11746030

Gene Structure 2205 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1141300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGAAGACAATGTTTTCTCTCAACAATCCCAGAGAAATGCCCACAACGGTGTCGTCTTTGTTCTCAGCCTACGCCTCCTTCGCCGGATCCATGATGCTGGTCCGTTCCATTGCCAGAGACCTCTTCCCACAGCCCCTCAGCTCATACATTTTCTCAAAACTCTCTCGCCTTTTCAGTACCCCTTTCTCCTCCATAACTCTCGTCATAGAAGAGTTCTCCGGCGCCGCACGCAACCAGGTCTACGCCGCCGCCGAGGTCTACCTCCGGACCATCAACAACATTACTCCTTCCACTCACCGCCTCTGTGTCGGAAAAAATATCCGGCAAAAGACAGTCAGCCTCGCCCTTGACAACAGCGGACAGCTCGAAGACGCCTTTGACAATGTGAATCTGACGTGGCGTTTCAAGGTGAGGAAGGAAAAGTCTGGTGCTGAACAGCGTCAGTTCGAGCTGAGCTTCCACAAAAAACACAAAGACAAAGTGATGGAGTCCTACTTCCCCTACGTGCTTGCTAGGGCTGACGCCATCAAAGAAGAGCAAAGGGTTGTGCAGCTTTATTCCCGCCATTTAATGTCTGATACTGATTTCAAAGGCAGGTGCACATGGGGTTCGGTGAATCTGGAGCACCCGTCTACGTTCGACATGATGGAGATGGACCCCGTGACGAAGAGGATGATTGTGGAGGATTTAGAGAGGTTTGTAAGGAGGAGGGAGTTTTACAAGAAGGTGGGGAAGGCTTGGAAAAGAGGCTACTTGCTGTATGGTTCTCCCGGGACCGGAAAATCCAGCTTGATTGCCGCCATGGCTAATTATCTCAAGTTTGATGTGTATGATTTGGAGCTCACTAGCATTCAAAACAACAATGAGCTGAGGAGGGTTTTGTTGTCCATGACAAATCGTTCGATTTTGGTGATTGAGGATATCGATTGCTCTGTGGATATACAGAACGGGGAATCGAATAAAAGGTCTCAAGATATCTCTCAACGATCTGCTTCTAACAAGTTGACACTTTCAGGCCTACTGAATTTCACCGATGGGCTGTGGTCGAGCTGCGGCGACGAGAGAATTATCGTGTTCACAACAAACCACAAGGAACAGCTAGACCCTGCATTGTTGCGTCCAGGTCGAATGGACCTGCACATTTACATGTCCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

386

Amino Acids

44.18

Weight (kDa)

9.24

Isoelectric Point (pI)

49.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	42 - 135	4.1e-19	Domain associated at C-terminal with AAA
AAA	PF00004	251 - 384	1.4e-17	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18190 AT2G18193
fragaria_vesca	FvH4_1g12130 FvH4_1g12690 FvH4_1g12700 FvH4_1g12712 FvH4_1g12716 FvH4_1g12731
malus_domestica	MD02G1141300.v1.1 MD02G1141400.v1.1 MD02G1141600.v1.1 MD02G1141800.v1.1 MD02G1142200.v1.1 MD15G1254200.v1.1
prunus_persica	Prupe.7G159500_v2.0.a1 Prupe.7G159600_v2.0.a1 Prupe.7G165500_v2.0.a1
pyrus_communis	pycom02g11130 pycom02g11150 pycom02g11160 pycom02g11180 pycom15g22350
rosa_chinensis	RchiOBHm_Chr2g0099791 RchiOBHm_Chr2g0099801 RchiOBHm_Chr2g0100651 RchiOBHm_Chr2g0100681 RchiOBHm_Chr2g0100691 RchiOBHm_Chr2g0100701 RchiOBHm_Chr2g0100721 RchiOBHm_Chr2g0100801 RchiOBHm_Chr3g0459141
rosa_laevigata	RLG00000016914 RLG00000016915 RLG00000016997 RLG00000016998 RLG00000017000 RLG00000017002 RLG00000017012 RLG00000025114
rosa_multiflora	Rmu_co8416275.1_g000001 Rmu_sc0001187.1_g000004 Rmu_sc0001497.1_g000006 Rmu_sc0001659.1_g000004 Rmu_sc0001659.1_g000006 Rmu_sc0001659.1_g000008 Rmu_sc0001659.1_g000010 Rmu_sc0008864.1_g000008 Rmu_sc0028022.1_g000001
rosa_roxburghii	Rroxscaffold_2G00141850 Rroxscaffold_2G00141930 Rroxscaffold_2G00141940 Rroxscaffold_2G00141950 Rroxscaffold_2G00141960 Rroxscaffold_2G00141970
rosa_rugosa	Rorug02G0091800 Rorug02G0091800 Rorug02G0091800 Rorug02G0091900 Rorug02G0092900 Rorug03G0033500
rosa_samantha	Rh2BG135600 Rh2BG143900 Rh2BG144000 Rh2BG144200 Rh2BG144300 Rh2BG144400 Rh2BG144500 Rh2BG145500 Rh2CG136900 Rh2CG137000 Rh2CG144800 Rh2CG145000 Rh2CG145100 Rh2CG145200 Rh2CG145500 Rh2CG146400 Rh2DG137400 Rh2DG137500 Rh2DG144300 Rh2DG144500 Rh2DG144600 Rh2DG144700 Rh2DG145000 Rh2DG145900 Rh3BG094700 Rh3DG095300
rosa_wichuraiana	Rw2G010920 Rw2G010950 Rw2G010990 Rw3G007860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	585
Acc36I	ACCTGC	2 cut(s)	585, 1143
AccB1I	GGYRCC	1 cut(s)	221
AccI	GTMKAC	3 cut(s)	240, 258, 632
AccIII	TCCGGA	1 cut(s)	264
AciI	CCGC	8 cut(s)	225, 246, 249, 298, 357, 562, 798, 1053
AclWI	GGATC	2 cut(s)	81, 94
AcsI	RAATTY	1 cut(s)	1024
AcyI	GRCGYC	3 cut(s)	222, 372, 522
AfaI	GTAC	1 cut(s)	178
AfiI	CCNNNNNNNGG	1 cut(s)	776
AflIII	ACRYGT	1 cut(s)	1146
AgsI	TTSAA	3 cut(s)	406, 589, 854
AjiI	CACGTC	1 cut(s)	396
AjnI	CCWGG	2 cut(s)	234, 1120
AleI	CACNNNNGTG	1 cut(s)	47
Alw21I	GWGCWC	3 cut(s)	602, 627, 843
Alw26I	GTCTC	2 cut(s)	111, 972
Alw44I	GTGCAC	1 cut(s)	598
AlwI	GGATC	2 cut(s)	81, 94
Ama87I	CYCGRG	1 cut(s)	770
Aor13HI	TCCGGA	1 cut(s)	264
AoxI	GGCC	1 cut(s)	1015
ApaLI	GTGCAC	1 cut(s)	598
ApeKI	GCWGC	2 cut(s)	550, 1050
ApoI	RAATTY	1 cut(s)	1024
ArsI	GACNNNNNNTTYG	4 cut(s)	883, 915, 950, 982
AspLEI	GCGC	1 cut(s)	224
AspS9I	GGNCC	5 cut(s)	100, 267, 655, 774, 1132
AsuC2I	CCSGG	2 cut(s)	771, 772
AsuHPI	GGTGA	5 cut(s)	287, 421, 625, 922, 1021
AvaI	CYCGRG	1 cut(s)	770
AvaII	GGWCC	5 cut(s)	100, 267, 655, 774, 1132
BaeGI	GKGCMC	1 cut(s)	602
BamHI	GGATCC	1 cut(s)	86
BanI	GGYRCC	1 cut(s)	221
BanII	GRGCYC	1 cut(s)	843
BbsI	GAAGAC	2 cut(s)	11, 375
Bbv12I	GWGCWC	3 cut(s)	602, 627, 843
BbvCI	CCTCAGC	2 cut(s)	137, 869
BbvI	GCAGC	2 cut(s)	562, 1037
BccI	CCATC	6 cut(s)	278, 478, 533, 640, 646, 1028
BciT130I	CCWGG	2 cut(s)	236, 1122
BcnI	CCSGG	2 cut(s)	771, 772
BcoDI	GTCTC	2 cut(s)	111, 972
BfaI	CTAG	3 cut(s)	513, 846, 1100
BfoI	RGCGCY	1 cut(s)	225
BfuAI	ACCTGC	2 cut(s)	585, 1143
BglI	GCCNNNNNGGC	1 cut(s)	803
BisI	GCNGC	7 cut(s)	225, 246, 249, 551, 798, 1051, 1054
BlpI	GCTNAGC	1 cut(s)	452
BlsI	GCNGC	7 cut(s)	226, 247, 250, 552, 799, 1052, 1055
Bme1390I	CCNGG	4 cut(s)	236, 771, 772, 1122
Bme18I	GGWCC	5 cut(s)	100, 267, 655, 774, 1132
BmeT110I	CYCGRG	1 cut(s)	770
BmgBI	CACGTC	1 cut(s)	396
BmgT120I	GGNCC	5 cut(s)	100, 267, 655, 774, 1132
BmiI	GGNNCC	4 cut(s)	88, 223, 657, 775
BmrFI	CCNGG	4 cut(s)	236, 771, 772, 1122
BmsI	GCATC	1 cut(s)	84
BpiI	GAAGAC	2 cut(s)	11, 375
BpmI	CTGGAG	1 cut(s)	641
Bpu10I	CCTNAGC	2 cut(s)	137, 869
Bpu1102I	GCTNAGC	1 cut(s)	452
BpuEI	CTTGAG	2 cut(s)	800, 954
BpuMI	CCSGG	2 cut(s)	771, 772
Bsa29I	ATCGAT	1 cut(s)	924
BsaAI	YACGTR	1 cut(s)	505
BsaHI	GRCGYC	3 cut(s)	222, 372, 522
BsaI	GGTCTC	2 cut(s)	111, 972
BsaJI	CCNNGG	3 cut(s)	252, 770, 801
BsaWI	WCCGGW	2 cut(s)	264, 776
BsaXI	ACNNNNNCTCC	2 cut(s)	614, 644
Bsc4I	CCNNNNNNNGG	1 cut(s)	776
Bse3DI	GCAATG	1 cut(s)	108
BseAI	TCCGGA	1 cut(s)	264
BseBI	CCWGG	2 cut(s)	236, 1122
BseCI	ATCGAT	1 cut(s)	924
BseDI	CCNNGG	3 cut(s)	252, 770, 801
BseGI	GGATG	1 cut(s)	678
BseLI	CCNNNNNNNGG	1 cut(s)	776
BseMI	GCAATG	1 cut(s)	108
BseMII	CTCAG	4 cut(s)	78, 151, 443, 860
BseRI	GAGGAG	3 cut(s)	178, 721, 886
BseSI	GKGCMC	1 cut(s)	602
BseXI	GCAGC	2 cut(s)	562, 1037
BsgI	GTGCAG	2 cut(s)	569, 1121
BshFI	GGCC	1 cut(s)	1017
BshNI	GGYRCC	1 cut(s)	221
BshVI	ATCGAT	1 cut(s)	924
BsiHKAI	GWGCWC	3 cut(s)	602, 627, 843
BsiHKCI	CYCGRG	1 cut(s)	770
BsiSI	CCGG	6 cut(s)	84, 219, 265, 322, 771, 777
BslFI	GGGAC	1 cut(s)	787
BslI	CCNNNNNNNGG	1 cut(s)	776
BsmAI	GTCTC	2 cut(s)	111, 972
BsmFI	GGGAC	1 cut(s)	787
BsmI	GAATGC	1 cut(s)	849
BsnI	GGCC	1 cut(s)	1017
Bso31I	GGTCTC	2 cut(s)	111, 972
BsoBI	CYCGRG	1 cut(s)	770
Bsp1286I	GDGCHC	3 cut(s)	602, 627, 843
Bsp13I	TCCGGA	1 cut(s)	264
Bsp143I	GATC	2 cut(s)	86, 986
Bsp1720I	GCTNAGC	1 cut(s)	452
Bsp19I	CCATGG	1 cut(s)	801
BspACI	CCGC	8 cut(s)	225, 246, 249, 298, 357, 562, 798, 1053
BspANI	GGCC	1 cut(s)	1017
BspCNI	CTCAG	4 cut(s)	77, 150, 444, 861
BspDI	ATCGAT	1 cut(s)	924
BspEI	TCCGGA	1 cut(s)	264
BspLI	GGNNCC	4 cut(s)	88, 223, 657, 775
BspMI	ACCTGC	2 cut(s)	585, 1143
BspPI	GGATC	2 cut(s)	81, 94
BspQI	GCTCTTC	1 cut(s)	528
BspT107I	GGYRCC	1 cut(s)	221
BspTNI	GGTCTC	2 cut(s)	111, 972
BsrDI	GCAATG	1 cut(s)	108
BssECI	CCNNGG	3 cut(s)	252, 770, 801
BssMI	GATC	2 cut(s)	86, 986
BssNI	GRCGYC	3 cut(s)	222, 372, 522
BssT1I	CCWWGG	1 cut(s)	801
Bst2UI	CCWGG	2 cut(s)	236, 1122
Bst4CI	ACNGT	2 cut(s)	49, 334
Bst6I	CTCTTC	4 cut(s)	128, 204, 528, 662
BstACI	GRCGYC	3 cut(s)	222, 372, 522
BstBAI	YACGTR	1 cut(s)	505
BstC8I	GCNNGC	2 cut(s)	229, 510
BstDEI	CTNAG	4 cut(s)	64, 137, 452, 869
BstDSI	CCRYGG	1 cut(s)	801
BstF5I	GGATG	1 cut(s)	678
BstH2I	RGCGCY	1 cut(s)	225
BstHHI	GCGC	1 cut(s)	224
BstKTI	GATC	2 cut(s)	89, 989
BstMAI	GTCTC	2 cut(s)	111, 972
BstMBI	GATC	2 cut(s)	86, 986
BstMWI	GCNNNNNNNGC	3 cut(s)	80, 228, 803
BstNI	CCWGG	2 cut(s)	236, 1122
BstNSI	RCATGY	1 cut(s)	1150
BstSCI	CCNGG	4 cut(s)	234, 769, 770, 1120
BstSLI	GKGCMC	1 cut(s)	602
BstV1I	GCAGC	2 cut(s)	562, 1037
BstV2I	GAAGAC	2 cut(s)	11, 375
BstX2I	RGATCY	1 cut(s)	86
BstXI	CCANNNNNNTGG	1 cut(s)	97
BstYI	RGATCY	1 cut(s)	86
Bsu15I	ATCGAT	1 cut(s)	924
BsuRI	GGCC	1 cut(s)	1017
BsuTUI	ATCGAT	1 cut(s)	924
BtgI	CCRYGG	1 cut(s)	801
BtrI	CACGTC	1 cut(s)	396
BtsCI	GGATG	1 cut(s)	678
BveI	ACCTGC	2 cut(s)	585, 1143
Cac8I	GCNNGC	2 cut(s)	229, 510
CfoI	GCGC	1 cut(s)	224
Cfr13I	GGNCC	5 cut(s)	100, 267, 655, 774, 1132
Cfr9I	CCCGGG	1 cut(s)	770
ClaI	ATCGAT	1 cut(s)	924
CseI	GACGC	4 cut(s)	380, 428, 530, 1106
CsiI	ACCWGGT	1 cut(s)	234
Csp6I	GTAC	1 cut(s)	177
CviAII	CATG	6 cut(s)	91, 603, 643, 802, 889, 1147
CviQI	GTAC	1 cut(s)	177
DdeI	CTNAG	4 cut(s)	64, 137, 452, 869
DinI	GGCGCC	1 cut(s)	223
DpnI	GATC	2 cut(s)	88, 988
DpnII	GATC	2 cut(s)	86, 986
Eam1104I	CTCTTC	4 cut(s)	128, 204, 528, 662
EarI	CTCTTC	4 cut(s)	128, 204, 528, 662
Ecl136II	GAGCTC	1 cut(s)	841
Eco130I	CCWWGG	1 cut(s)	801
Eco147I	AGGCCT	1 cut(s)	1017
Eco24I	GRGCYC	1 cut(s)	843
Eco31I	GGTCTC	2 cut(s)	111, 972
Eco32I	GATATC	2 cut(s)	922, 976
Eco47I	GGWCC	5 cut(s)	100, 267, 655, 774, 1132
Eco53kI	GAGCTC	1 cut(s)	841
Eco88I	CYCGRG	1 cut(s)	770
EcoICRI	GAGCTC	1 cut(s)	841
EcoRII	CCWGG	2 cut(s)	234, 1120
EcoRV	GATATC	2 cut(s)	922, 976
EcoT14I	CCWWGG	1 cut(s)	801
EcoT38I	GRGCYC	1 cut(s)	843
EgeI	GGCGCC	1 cut(s)	223
EheI	GGCGCC	1 cut(s)	223
ErhI	CCWWGG	1 cut(s)	801
FaeI	CATG	6 cut(s)	94, 606, 646, 805, 892, 1150
FaqI	GGGAC	1 cut(s)	787
FatI	CATG	6 cut(s)	90, 602, 642, 801, 888, 1146
FauI	CCCGC	1 cut(s)	569
FblI	GTMKAC	3 cut(s)	240, 258, 632
Fnu4HI	GCNGC	7 cut(s)	225, 246, 249, 551, 798, 1051, 1054
FokI	GGATG	1 cut(s)	685
FriOI	GRGCYC	1 cut(s)	843
Fsp4HI	GCNGC	7 cut(s)	225, 246, 249, 551, 798, 1051, 1054
FspBI	CTAG	3 cut(s)	513, 846, 1100
GlaI	GCGC	1 cut(s)	223
GluI	GCNGC	7 cut(s)	225, 246, 249, 551, 798, 1051, 1054
GsuI	CTGGAG	1 cut(s)	641
HaeII	RGCGCY	1 cut(s)	225
HaeIII	GGCC	1 cut(s)	1017
HapII	CCGG	6 cut(s)	84, 219, 265, 322, 771, 777
HgaI	GACGC	4 cut(s)	380, 428, 530, 1106
HhaI	GCGC	1 cut(s)	224
Hin1I	GRCGYC	3 cut(s)	222, 372, 522
Hin1II	CATG	6 cut(s)	94, 606, 646, 805, 892, 1150
Hin6I	GCGC	1 cut(s)	222
HinP1I	GCGC	1 cut(s)	222
HincII	GTYRAC	1 cut(s)	1005
HindII	GTYRAC	1 cut(s)	1005
HinfI	GANTC	4 cut(s)	388, 488, 616, 953
HpaII	CCGG	6 cut(s)	84, 219, 265, 322, 771, 777
HphI	GGTGA	5 cut(s)	287, 421, 625, 922, 1021
Hpy166II	GTNNAC	6 cut(s)	241, 259, 600, 633, 1005, 1077
Hpy188I	TCNGA	3 cut(s)	311, 393, 577
Hpy188III	TCNNGA	3 cut(s)	265, 620, 971
Hpy8I	GTNNAC	6 cut(s)	241, 259, 600, 633, 1005, 1077
Hpy99I	CGWCG	1 cut(s)	1061
HpyAV	CCTTC	5 cut(s)	88, 297, 409, 718, 727
HpyCH4III	ACNGT	2 cut(s)	49, 334
HpyCH4IV	ACGT	3 cut(s)	395, 504, 635
HpyCH4V	TGCA	4 cut(s)	550, 600, 1109, 1138
HpyF10VI	GCNNNNNNNGC	3 cut(s)	80, 228, 803
HpyF3I	CTNAG	4 cut(s)	64, 137, 452, 869
HpySE526I	ACGT	3 cut(s)	395, 504, 635
Hsp92I	GRCGYC	3 cut(s)	222, 372, 522
Hsp92II	CATG	6 cut(s)	94, 606, 646, 805, 892, 1150
HspAI	GCGC	1 cut(s)	222
KasI	GGCGCC	1 cut(s)	221
Kpn2I	TCCGGA	1 cut(s)	264
Kzo9I	GATC	2 cut(s)	86, 986
LguI	GCTCTTC	1 cut(s)	528
LmnI	GCTCC	2 cut(s)	622, 838
Lsp1109I	GCAGC	2 cut(s)	562, 1037
LweI	GCATC	1 cut(s)	84
MabI	ACCWGGT	1 cut(s)	234
MaeI	CTAG	3 cut(s)	513, 846, 1100
MaeII	ACGT	3 cut(s)	395, 504, 635
MaeIII	GTNAC	1 cut(s)	661
MalI	GATC	2 cut(s)	88, 988
MboI	GATC	2 cut(s)	86, 986
MboII	GAAGA	6 cut(s)	16, 115, 221, 380, 545, 679
MflI	RGATCY	1 cut(s)	86
MhlI	GDGCHC	3 cut(s)	602, 627, 843
MluCI	AATT	3 cut(s)	808, 1024, 1065
Mly113I	GGCGCC	1 cut(s)	222
MlyI	GAGTC	1 cut(s)	497
MmeI	TCCRAC	1 cut(s)	289
MroI	TCCGGA	1 cut(s)	264
MseI	TTAA	1 cut(s)	569
MslI	CAYNNNNRTG	2 cut(s)	47, 1145
MspA1I	CMGCKG	1 cut(s)	357
MspI	CCGG	6 cut(s)	84, 219, 265, 322, 771, 777
MspR9I	CCNGG	4 cut(s)	236, 771, 772, 1122
Mva1269I	GAATGC	1 cut(s)	849
MvaI	CCWGG	2 cut(s)	236, 1122
MwoI	GCNNNNNNNGC	3 cut(s)	80, 228, 803
NarI	GGCGCC	1 cut(s)	222
NciI	CCSGG	2 cut(s)	771, 772
NcoI	CCATGG	1 cut(s)	801
NdeII	GATC	2 cut(s)	86, 986
NlaIII	CATG	6 cut(s)	94, 606, 646, 805, 892, 1150
NlaIV	GGNNCC	4 cut(s)	88, 223, 657, 775
NmeAIII	GCCGAG	1 cut(s)	277
NmuCI	GTSAC	1 cut(s)	661
NspI	RCATGY	1 cut(s)	1150
OliI	CACNNNNGTG	1 cut(s)	47
PaqCI	CACCTGC	1 cut(s)	585
PceI	AGGCCT	1 cut(s)	1017
PciI	ACATGT	1 cut(s)	1146
PciSI	GCTCTTC	1 cut(s)	528
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PctI	GAATGC	1 cut(s)	849
PfeI	GAWTC	3 cut(s)	388, 616, 953
PkrI	GCNGC	7 cut(s)	226, 247, 250, 552, 799, 1052, 1055
PleI	GAGTC	1 cut(s)	496
PluTI	GGCGCC	1 cut(s)	225
PpsI	GAGTC	1 cut(s)	496
Ppu21I	YACGTR	1 cut(s)	505
PscI	ACATGT	1 cut(s)	1146
Psp124BI	GAGCTC	1 cut(s)	843
Psp6I	CCWGG	2 cut(s)	234, 1120
PspGI	CCWGG	2 cut(s)	234, 1120
PspN4I	GGNNCC	4 cut(s)	88, 223, 657, 775
PspPI	GGNCC	5 cut(s)	100, 267, 655, 774, 1132
PsuI	RGATCY	1 cut(s)	86
RsaI	GTAC	1 cut(s)	178
RsaNI	GTAC	1 cut(s)	177
RseI	CAYNNNNRTG	2 cut(s)	47, 1145
SacI	GAGCTC	1 cut(s)	843
SapI	GCTCTTC	1 cut(s)	528
SaqAI	TTAA	1 cut(s)	569
SatI	GCNGC	7 cut(s)	225, 246, 249, 551, 798, 1051, 1054
Sau3AI	GATC	2 cut(s)	86, 986
Sau96I	GGNCC	5 cut(s)	100, 267, 655, 774, 1132
SchI	GAGTC	1 cut(s)	497
ScrFI	CCNGG	4 cut(s)	236, 771, 772, 1122
SduI	GDGCHC	3 cut(s)	602, 627, 843
SexAI	ACCWGGT	1 cut(s)	234
SfaNI	GCATC	1 cut(s)	84
SfoI	GGCGCC	1 cut(s)	223
SinI	GGWCC	5 cut(s)	100, 267, 655, 774, 1132
SmaI	CCCGGG	1 cut(s)	772
SmiMI	CAYNNNNRTG	2 cut(s)	47, 1145
SmlI	CTYRAG	2 cut(s)	815, 969
SmoI	CTYRAG	2 cut(s)	815, 969
Sse9I	AATT	3 cut(s)	808, 1024, 1065
SseBI	AGGCCT	1 cut(s)	1017
SsiI	CCGC	8 cut(s)	225, 246, 249, 298, 357, 562, 798, 1053
SspDI	GGCGCC	1 cut(s)	221
SspMI	CTAG	3 cut(s)	513, 846, 1100
SstI	GAGCTC	1 cut(s)	843
StuI	AGGCCT	1 cut(s)	1017
StyD4I	CCNGG	4 cut(s)	234, 769, 770, 1120
StyI	CCWWGG	1 cut(s)	801
TaaI	ACNGT	2 cut(s)	49, 334
TaiI	ACGT	3 cut(s)	398, 507, 638
TaqI	TCGA	8 cut(s)	366, 447, 639, 902, 924, 956, 1046, 1126
TasI	AATT	3 cut(s)	808, 1024, 1065
TauI	GCSGC	5 cut(s)	227, 248, 251, 800, 1056
TfiI	GAWTC	3 cut(s)	388, 616, 953
Tru1I	TTAA	1 cut(s)	569
Tru9I	TTAA	1 cut(s)	569
TseFI	GTSAC	1 cut(s)	661
TseI	GCWGC	2 cut(s)	550, 1050
Tsp45I	GTSAC	1 cut(s)	661
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	1 cut(s)	92
TspMI	CCCGGG	1 cut(s)	770
VneI	GTGCAC	1 cut(s)	598
VpaK11BI	GGWCC	5 cut(s)	100, 267, 655, 774, 1132
XapI	RAATTY	1 cut(s)	1024
XceI	RCATGY	1 cut(s)	1150
XmaI	CCCGGG	1 cut(s)	770
XmiI	GTMKAC	3 cut(s)	240, 258, 632
XspI	CTAG	3 cut(s)	513, 846, 1100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.