Rroxscaffold_2G00141970

Domain associated at C-terminal with AAA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000471
Physical Location & Seq
Reverse (-)
80152754 .. 80154208
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141970.1

Sequence Viewer

Length: 1455 bp
ATGCCCACAACAGCATCGTCCTTGTTCTCAGCCTATGCCTCCCTCACCGCATTCATAATGTTGGTCCGTTCCATAGTTGATCAGTTGATCCCCCACGAGCTCCGCTACTACATCTACTCGGCCTTCGGCCGCCTCTTCACCCCTCGCCCCTCCAACCTCACTCTCATCGTCGATGAGTGCTGCGGCATGGTTCAAAACCAAATCTATGAGGCGGCGGAGGTCTACCTCCGGACCAAGATCAGTCCCCTAAACGACCGTCTCCGGGTGAAAAAGGCACCTCGGCAGAAGAACATTACCGTCACGATTGAAAAGGGTGAAGAAATCAAAGACAGTTTCGACGGGATAAACATCAACTGGCGTTTTGTGTGCAGCGGAGGAGAGAATGAGCAGAACAGCCATGCGCAACAGAAGCGTCAGTTTGAGCTGACAGTGAATAAGAAACATCGAGTCACAGTAATTGAGTCCTACTTGCCGTATGTGCTTGACAGGGCCGAGGCCATCAAGAATGAGGACAAGGTTGTGAAGCTCTATAACCAAGAGCGGTCATATGATCCGTATGATGATGAATCCAATTCGGACTGGAGTTCGATAAATCTGGAGCATCCGGCTACCTTTGAGACACTTGCCATGGATGCCGAGGTTAAGAGAACGATCATTCAGGATTTGGATAGGTTTTTGAGGAGGAGGGAGTTTTATAAGAAGGTTGGAAAGGCCTGGAAAAGAGGGTATCTGTTGTATGGTCCACCCGGGACTGGCAAATCCAGCTTGATTGCTGCCATGGCTAATTATCTCAAGTTTGATATATACGATGTGGAGCTTACCAGTATTGAAGACAACTCGGAGTTGAGGAGGATACTGCTGACTACGACAAACCGTTCTATCTTGGTCATTGAGGATATTGATTGCAGTGTGGAGGTGCAAAACCGGGAATGTGAGGAGGAAGATCATCAACCATCTAACACCAAGTTGACACTCTCCGGCCTACTGAACTTTATAGATGGTTTGTGGTCAAGCTGCGGAGATGAGAGAATCATTGTGTTCACCACCAACCACAAGCACAAGATAGACCCGGCATTGTTGCGTCCTGGTCGAATGGATGTGCACATTCACATGTCATATTGCACTCCAAGCGGGTTCAGGATCTTGGCCTCTAACTACCTTGGTATGCATGAAAGCAATCCTCATCGCCTATGCGGAGAAGTTGAGGAGTTAATAGAGAACTCAAAGGTCACCCCCGCGGAGATTGCTGAGCAGCTCATGAAGAGTGAGGATGCTGATGTTGCTCTTGAAGGACTTGTCGATTTCCTGAAAAGAAAGAAGGAAGAGAGCAATAAAATTGGGGATGAAGGGACTAATAAATCTGAAATTCAAGAAACAGATCAAAACGCAGAAAAGTGGAGCTCAGTCAGTAGCGATGATGAACTTAAATCTTCTCTTTCAGATGCTAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

484

Amino Acids

55.32

Weight (kDa)

5.32

Isoelectric Point (pI)

53.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 30 - 123 2e-25 Domain associated at C-terminal with AAA
AAA PF00004 243 - 372 1.7e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 374 - 444 6.8e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000471)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18190 AT2G18193
fragaria_vesca FvH4_1g12130 FvH4_1g12690 FvH4_1g12700 FvH4_1g12712 FvH4_1g12716 FvH4_1g12731
malus_domestica MD02G1141300.v1.1 MD02G1141400.v1.1 MD02G1141600.v1.1 MD02G1141800.v1.1 MD02G1142200.v1.1 MD15G1254200.v1.1
prunus_persica Prupe.7G159500_v2.0.a1 Prupe.7G159600_v2.0.a1 Prupe.7G165500_v2.0.a1
pyrus_communis pycom02g11130 pycom02g11150 pycom02g11160 pycom02g11180 pycom15g22350
rosa_chinensis RchiOBHm_Chr2g0099791 RchiOBHm_Chr2g0099801 RchiOBHm_Chr2g0100651 RchiOBHm_Chr2g0100681 RchiOBHm_Chr2g0100691 RchiOBHm_Chr2g0100701 RchiOBHm_Chr2g0100721 RchiOBHm_Chr2g0100801 RchiOBHm_Chr3g0459141
rosa_laevigata RLG00000016914 RLG00000016915 RLG00000016997 RLG00000016998 RLG00000017000 RLG00000017002 RLG00000017012 RLG00000025114
rosa_multiflora Rmu_co8416275.1_g000001 Rmu_sc0001187.1_g000004 Rmu_sc0001497.1_g000006 Rmu_sc0001659.1_g000004 Rmu_sc0001659.1_g000006 Rmu_sc0001659.1_g000008 Rmu_sc0001659.1_g000010 Rmu_sc0008864.1_g000008 Rmu_sc0028022.1_g000001
rosa_roxburghii Rroxscaffold_2G00141850 Rroxscaffold_2G00141930 Rroxscaffold_2G00141940 Rroxscaffold_2G00141950 Rroxscaffold_2G00141960 Rroxscaffold_2G00141970
rosa_rugosa Rorug02G0091800 Rorug02G0091800 Rorug02G0091800 Rorug02G0091900 Rorug02G0092900 Rorug03G0033500
rosa_samantha Rh2BG135600 Rh2BG143900 Rh2BG144000 Rh2BG144200 Rh2BG144300 Rh2BG144400 Rh2BG144500 Rh2BG145500 Rh2CG136900 Rh2CG137000 Rh2CG144800 Rh2CG145000 Rh2CG145100 Rh2CG145200 Rh2CG145500 Rh2CG146400 Rh2DG137400 Rh2DG137500 Rh2DG144300 Rh2DG144500 Rh2DG144600 Rh2DG144700 Rh2DG145000 Rh2DG145900 Rh3BG094700 Rh3DG095300
rosa_wichuraiana Rw2G010920 Rw2G010950 Rw2G010990 Rw3G007860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
Acc16I TGCGCA 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 541
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 1238
AccIII TCCGGA 1 cut(s) 228
AclWI GGATC 3 cut(s) 82, 545, 1148
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 1365
AfiI CCNNNNNNNGG 2 cut(s) 262, 752
AflIII ACRYGT 1 cut(s) 1110
AgsI TTSAA 5 cut(s) 194, 308, 830, 1289, 1370
AjnI CCWGG 2 cut(s) 713, 1084
AluBI AGCT 8 cut(s) 100, 424, 526, 765, 817, 1014, 1255, 1401
AluI AGCT 8 cut(s) 100, 424, 526, 765, 817, 1014, 1255, 1401
Alw21I GWGCWC 3 cut(s) 102, 1104, 1403
Alw26I GTCTC 2 cut(s) 263, 611
Alw44I GTGCAC 1 cut(s) 1100
AlwI GGATC 3 cut(s) 82, 545, 1148
Ama87I CYCGRG 1 cut(s) 746
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 7 cut(s) 120, 127, 489, 495, 711, 979, 1146
ApaLI GTGCAC 1 cut(s) 1100
ApeKI GCWGC 5 cut(s) 180, 369, 773, 1014, 1252
ApoI RAATTY 1 cut(s) 1365
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 4 cut(s) 64, 231, 489, 740
AsuC2I CCSGG 5 cut(s) 263, 747, 748, 926, 1070
AsuHPI GGTGA 6 cut(s) 37, 130, 277, 326, 1033, 1222
AvaI CYCGRG 1 cut(s) 746
AvaII GGWCC 3 cut(s) 64, 231, 740
BaeGI GKGCMC 1 cut(s) 1104
BanI GGYRCC 1 cut(s) 274
BanII GRGCYC 2 cut(s) 102, 1403
BauI CACGAG 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 3 cut(s) 102, 1104, 1403
BbvI GCAGC 5 cut(s) 167, 381, 760, 1001, 1264
BccI CCATC 3 cut(s) 506, 961, 992
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 2 cut(s) 715, 1086
BciVI GTATCC 1 cut(s) 846
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 5 cut(s) 263, 747, 748, 926, 1070
BcoDI GTCTC 2 cut(s) 263, 611
BfuI GTATCC 1 cut(s) 846
BisI GCNGC 8 cut(s) 130, 181, 184, 213, 370, 774, 1015, 1253
BlpI GCTNAGC 1 cut(s) 1248
BlsI GCNGC 8 cut(s) 131, 182, 185, 214, 371, 775, 1016, 1254
Bme1390I CCNGG 7 cut(s) 263, 715, 747, 748, 926, 1070, 1086
Bme18I GGWCC 3 cut(s) 64, 231, 740
BmeT110I CYCGRG 1 cut(s) 746
BmgT120I GGNCC 4 cut(s) 64, 231, 489, 740
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 7 cut(s) 263, 715, 747, 748, 926, 1070, 1086
BmsI GCATC 5 cut(s) 23, 610, 622, 1261, 1432
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 2 cut(s) 601, 617
Bpu1102I GCTNAGC 1 cut(s) 1248
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 5 cut(s) 263, 747, 748, 926, 1070
BsaBI GATNNNNATC 1 cut(s) 347
BsaJI CCNNGG 8 cut(s) 278, 492, 627, 636, 746, 777, 1159, 1236
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 752
Bse1I ACTGG 4 cut(s) 359, 584, 757, 822
Bse8I GATNNNNATC 1 cut(s) 347
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 2 cut(s) 715, 1086
BseDI CCNNGG 8 cut(s) 278, 492, 627, 636, 746, 777, 1159, 1236
BseGI GGATG 5 cut(s) 601, 637, 1102, 1276, 1348
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 2 cut(s) 262, 752
BseMII CTCAG 3 cut(s) 42, 1239, 1416
BseNI ACTGG 4 cut(s) 359, 584, 757, 822
BseRI GAGGAG 6 cut(s) 390, 694, 697, 862, 950, 1220
BseSI GKGCMC 1 cut(s) 1104
BseX3I CGGCCG 1 cut(s) 127
BseXI GCAGC 5 cut(s) 167, 381, 760, 1001, 1264
BsgI GTGCAG 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 1238
Bsh1285I CGRYCG 2 cut(s) 130, 256
BshFI GGCC 7 cut(s) 122, 129, 491, 497, 713, 981, 1148
BshNI GGYRCC 1 cut(s) 274
BsiEI CGRYCG 2 cut(s) 130, 256
BsiHKAI GWGCWC 3 cut(s) 102, 1104, 1403
BsiHKCI CYCGRG 1 cut(s) 746
BsiSI CCGG 7 cut(s) 229, 262, 605, 747, 925, 978, 1070
BslFI GGGAC 3 cut(s) 228, 763, 1363
BslI CCNNNNNNNGG 2 cut(s) 262, 752
BsmAI GTCTC 2 cut(s) 263, 611
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 3 cut(s) 228, 763, 1363
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 7 cut(s) 122, 129, 491, 497, 713, 981, 1148
BsoBI CYCGRG 1 cut(s) 746
Bsp1286I GDGCHC 3 cut(s) 102, 1104, 1403
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
Bsp1720I GCTNAGC 1 cut(s) 1248
Bsp19I CCATGG 2 cut(s) 627, 777
BspANI GGCC 7 cut(s) 122, 129, 491, 497, 713, 981, 1148
BspCNI CTCAG 3 cut(s) 41, 1240, 1415
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 1 cut(s) 1238
BspHI TCATGA 1 cut(s) 1257
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 3 cut(s) 82, 545, 1148
BspT107I GGYRCC 1 cut(s) 274
BsrBI CCGCTC 1 cut(s) 541
BsrI ACTGG 4 cut(s) 359, 584, 757, 822
BssECI CCNNGG 8 cut(s) 278, 492, 627, 636, 746, 777, 1159, 1236
BssMI GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 3 cut(s) 627, 777, 1159
Bst2BI CACGAG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 715, 1086
Bst4CI ACNGT 6 cut(s) 257, 298, 332, 430, 454, 875
Bst6I CTCTTC 3 cut(s) 140, 1256, 1317
BstDEI CTNAG 3 cut(s) 28, 1248, 1402
BstDSI CCRYGG 3 cut(s) 627, 777, 1236
BstEII GGTNACC 1 cut(s) 1228
BstF5I GGATG 5 cut(s) 601, 637, 1102, 1276, 1348
BstFNI CGCG 1 cut(s) 1238
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 8 cut(s) 82, 90, 240, 553, 654, 946, 1143, 1381
BstMAI GTCTC 2 cut(s) 263, 611
BstMBI GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
BstMCI CGRYCG 2 cut(s) 130, 256
BstMWI GCNNNNNNNGC 8 cut(s) 409, 478, 632, 762, 779, 1128, 1244, 1280
BstNI CCWGG 2 cut(s) 715, 1086
BstNSI RCATGY 1 cut(s) 1114
BstPI GGTNACC 1 cut(s) 1228
BstSCI CCNGG 7 cut(s) 261, 713, 745, 746, 924, 1068, 1084
BstSLI GKGCMC 1 cut(s) 1104
BstUI CGCG 1 cut(s) 1238
BstV1I GCAGC 5 cut(s) 167, 381, 760, 1001, 1264
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 1140
BstYI RGATCY 1 cut(s) 1140
BstZI CGGCCG 1 cut(s) 127
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 7 cut(s) 122, 129, 491, 497, 713, 981, 1148
BtgI CCRYGG 3 cut(s) 627, 777, 1236
BtgZI GCGATG 2 cut(s) 1169, 1428
BtsCI GGATG 5 cut(s) 601, 637, 1102, 1276, 1348
BtsI GCAGTG 1 cut(s) 913
BtsIMutI CAGTG 2 cut(s) 435, 913
CciI TCATGA 1 cut(s) 1257
CfoI GCGC 1 cut(s) 403
Cfr13I GGNCC 4 cut(s) 64, 231, 489, 740
Cfr42I CCGCGG 1 cut(s) 1239
Cfr9I CCCGGG 1 cut(s) 746
CseI GACGC 2 cut(s) 401, 1070
CviAII CATG 7 cut(s) 187, 398, 628, 778, 1111, 1169, 1258
DdeI CTNAG 3 cut(s) 28, 1248, 1402
DpnI GATC 8 cut(s) 81, 89, 239, 552, 653, 945, 1142, 1380
DpnII GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
EaeI YGGCCR 1 cut(s) 127
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 140, 1256, 1317
EarI CTCTTC 3 cut(s) 140, 1256, 1317
EciI GGCGGA 1 cut(s) 230
Ecl136II GAGCTC 2 cut(s) 100, 1401
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 3 cut(s) 627, 777, 1159
Eco147I AGGCCT 1 cut(s) 713
Eco24I GRGCYC 2 cut(s) 102, 1403
Eco47I GGWCC 3 cut(s) 64, 231, 740
Eco52I CGGCCG 1 cut(s) 127
Eco53kI GAGCTC 2 cut(s) 100, 1401
Eco88I CYCGRG 1 cut(s) 746
Eco91I GGTNACC 1 cut(s) 1228
EcoICRI GAGCTC 2 cut(s) 100, 1401
EcoO65I GGTNACC 1 cut(s) 1228
EcoRII CCWGG 2 cut(s) 713, 1084
EcoT14I CCWWGG 3 cut(s) 627, 777, 1159
EcoT22I ATGCAT 1 cut(s) 1170
EcoT38I GRGCYC 2 cut(s) 102, 1403
ErhI CCWWGG 3 cut(s) 627, 777, 1159
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 7 cut(s) 190, 401, 631, 781, 1114, 1172, 1261
FaqI GGGAC 3 cut(s) 228, 763, 1363
FatI CATG 7 cut(s) 186, 397, 627, 777, 1110, 1168, 1257
FauI CCCGC 2 cut(s) 1124, 1243
FauNDI CATATG 1 cut(s) 547
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 8 cut(s) 130, 181, 184, 213, 370, 774, 1015, 1253
FokI GGATG 5 cut(s) 588, 644, 1109, 1283, 1355
FriOI GRGCYC 2 cut(s) 102, 1403
Fsp4HI GCNGC 8 cut(s) 130, 181, 184, 213, 370, 774, 1015, 1253
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 1 cut(s) 402
GluI GCNGC 8 cut(s) 130, 181, 184, 213, 370, 774, 1015, 1253
GsuI CTGGAG 2 cut(s) 601, 617
HaeIII GGCC 7 cut(s) 122, 129, 491, 497, 713, 981, 1148
HapII CCGG 7 cut(s) 229, 262, 605, 747, 925, 978, 1070
HgaI GACGC 2 cut(s) 401, 1070
HhaI GCGC 1 cut(s) 403
Hin1II CATG 7 cut(s) 190, 401, 631, 781, 1114, 1172, 1261
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 969
HindII GTYRAC 1 cut(s) 969
HinfI GANTC 4 cut(s) 447, 461, 566, 1029
HpaII CCGG 7 cut(s) 229, 262, 605, 747, 925, 978, 1070
HphI GGTGA 6 cut(s) 37, 130, 277, 326, 1033, 1222
Hpy166II GTNNAC 5 cut(s) 223, 743, 969, 1041, 1102
Hpy188I TCNGA 4 cut(s) 577, 841, 1363, 1441
Hpy8I GTNNAC 5 cut(s) 223, 743, 969, 1041, 1102
Hpy99I CGWCG 2 cut(s) 173, 341
HpyAV CCTTC 5 cut(s) 133, 694, 1283, 1312, 1340
HpyCH4III ACNGT 6 cut(s) 257, 298, 332, 430, 454, 875
HpyCH4V TGCA 6 cut(s) 369, 906, 919, 1102, 1122, 1168
HpyF10VI GCNNNNNNNGC 8 cut(s) 409, 478, 632, 762, 779, 1128, 1244, 1280
HpyF3I CTNAG 3 cut(s) 28, 1248, 1402
Hsp92II CATG 7 cut(s) 190, 401, 631, 781, 1114, 1172, 1261
HspAI GCGC 1 cut(s) 401
Kpn2I TCCGGA 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 79
KspI CCGCGG 1 cut(s) 1239
Kzo9I GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
LmnI GCTCC 4 cut(s) 105, 598, 814, 1398
Lsp1109I GCAGC 5 cut(s) 167, 381, 760, 1001, 1264
LweI GCATC 5 cut(s) 23, 610, 622, 1261, 1432
MaeIII GTNAC 3 cut(s) 298, 448, 1228
MalI GATC 8 cut(s) 81, 89, 239, 552, 653, 945, 1142, 1380
MbiI CCGCTC 1 cut(s) 541
MboI GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
MboII GAAGA 8 cut(s) 127, 298, 329, 842, 953, 1273, 1334, 1422
MflI RGATCY 1 cut(s) 1140
MhlI GDGCHC 3 cut(s) 102, 1104, 1403
MluCI AATT 5 cut(s) 456, 571, 784, 1335, 1365
MlyI GAGTC 2 cut(s) 456, 470
MmeI TCCRAC 2 cut(s) 177, 685
Mph1103I ATGCAT 1 cut(s) 1170
MroI TCCGGA 1 cut(s) 228
MseI TTAA 3 cut(s) 642, 1211, 1425
MslI CAYNNNNRTG 1 cut(s) 1109
MspA1I CMGCKG 2 cut(s) 372, 1238
MspI CCGG 7 cut(s) 229, 262, 605, 747, 925, 978, 1070
MspR9I CCNGG 7 cut(s) 263, 715, 747, 748, 926, 1070, 1086
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 2 cut(s) 715, 1086
MvnI CGCG 1 cut(s) 1238
MwoI GCNNNNNNNGC 8 cut(s) 409, 478, 632, 762, 779, 1128, 1244, 1280
NciI CCSGG 5 cut(s) 263, 747, 748, 926, 1070
NcoI CCATGG 2 cut(s) 627, 777
NdeI CATATG 1 cut(s) 547
NdeII GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
NlaIII CATG 7 cut(s) 190, 401, 631, 781, 1114, 1172, 1261
NlaIV GGNNCC 1 cut(s) 276
NmeAIII GCCGAG 4 cut(s) 98, 259, 517, 661
NmuCI GTSAC 3 cut(s) 298, 448, 1228
NsbI TGCGCA 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 1170
NspI RCATGY 1 cut(s) 1114
PagI TCATGA 1 cut(s) 1257
PceI AGGCCT 1 cut(s) 713
PciI ACATGT 1 cut(s) 1110
PctI GAATGC 1 cut(s) 50
PfeI GAWTC 2 cut(s) 566, 1029
PkrI GCNGC 8 cut(s) 131, 182, 185, 214, 371, 775, 1016, 1254
PleI GAGTC 2 cut(s) 455, 469
PpsI GAGTC 2 cut(s) 455, 469
PscI ACATGT 1 cut(s) 1110
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 2 cut(s) 102, 1403
Psp6I CCWGG 2 cut(s) 713, 1084
PspEI GGTNACC 1 cut(s) 1228
PspGI CCWGG 2 cut(s) 713, 1084
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 4 cut(s) 64, 231, 489, 740
PsuI RGATCY 1 cut(s) 1140
RseI CAYNNNNRTG 1 cut(s) 1109
SacI GAGCTC 2 cut(s) 102, 1403
SacII CCGCGG 1 cut(s) 1239
SaqAI TTAA 3 cut(s) 642, 1211, 1425
SatI GCNGC 8 cut(s) 130, 181, 184, 213, 370, 774, 1015, 1253
Sau3AI GATC 8 cut(s) 79, 87, 237, 550, 651, 943, 1140, 1378
Sau96I GGNCC 4 cut(s) 64, 231, 489, 740
SchI GAGTC 2 cut(s) 456, 470
ScrFI CCNGG 7 cut(s) 263, 715, 747, 748, 926, 1070, 1086
SduI GDGCHC 3 cut(s) 102, 1104, 1403
SfaNI GCATC 5 cut(s) 23, 610, 622, 1261, 1432
Sfr303I CCGCGG 1 cut(s) 1239
SgrBI CCGCGG 1 cut(s) 1239
SinI GGWCC 3 cut(s) 64, 231, 740
SmaI CCCGGG 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
Sse9I AATT 5 cut(s) 456, 571, 784, 1335, 1365
SseBI AGGCCT 1 cut(s) 713
SstI GAGCTC 2 cut(s) 102, 1403
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 7 cut(s) 261, 713, 745, 746, 924, 1068, 1084
StyI CCWWGG 3 cut(s) 627, 777, 1159
TaaI ACNGT 6 cut(s) 257, 298, 332, 430, 454, 875
TaqI TCGA 6 cut(s) 171, 336, 445, 587, 1090, 1299
TasI AATT 5 cut(s) 456, 571, 784, 1335, 1365
TauI GCSGC 3 cut(s) 132, 186, 215
TfiI GAWTC 2 cut(s) 566, 1029
Tru1I TTAA 3 cut(s) 642, 1211, 1425
Tru9I TTAA 3 cut(s) 642, 1211, 1425
TscAI CASTG 2 cut(s) 435, 913
TseFI GTSAC 3 cut(s) 298, 448, 1228
TseI GCWGC 5 cut(s) 180, 369, 773, 1014, 1252
Tsp45I GTSAC 3 cut(s) 298, 448, 1228
TspDTI ATGAA 6 cut(s) 43, 579, 1185, 1274, 1359, 1434
TspGWI ACGGA 2 cut(s) 56, 543
TspMI CCCGGG 1 cut(s) 746
TspRI CASTG 2 cut(s) 435, 913
VneI GTGCAC 1 cut(s) 1100
VpaK11BI GGWCC 3 cut(s) 64, 231, 740
XapI RAATTY 1 cut(s) 1365
XceI RCATGY 1 cut(s) 1114
XmaI CCCGGG 1 cut(s) 746
XmiI GTMKAC 1 cut(s) 222
Zsp2I ATGCAT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.