MD15G1254200.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000471
Physical Location & Seq
Reverse (-)
21475830 .. 21477970
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1254200.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGTTTTCTCTGGACAAACTGAAAGACCAGATGCCGACGACGGCGTCGTCGATGTTCTCCGTCTACGCTTCCGTCACCACATTCATGATGCTGTTCCGCTCCGTCGCCAGCGAGCTCATCCCCGCCCCCTTCCGCTCCTACATCTACAAAGCCCTGAATTACCTTTTTGCCCCTCTCTTCTCCCGTGACCTCACTCTCGTAGTCGATGAGCTCTCCGGCATGACCCGCAACCAAGTCTACGACGCGGCCGAGGTTTACCTGCGGACAAAGATCGGGCCCCACACCGAGCGCCTCCGCGTCAGCAAAGCTCCCCGGAAGAAGAACATCGGCGTCGCCATCGACAAGGACGAGGAAGTTTCCGACACCTTCGACGGTGCCGTCGAGCTCAAGTGGCGGTTTATCTCGGAGACCGAGAAGGGGAAGAACGACAGCACATCCGAAAGGCGGTATTTTGAGCTGACATTTCGTAGGAAATACATGGACAAGGTGATTTCCTCGTACCTGCCTTATGTTCTTGCTCAGGCAGACGCCATTAAAGCAGAGGAGAAGGCGGTGAAGCTCTACACCCGCCACCTTTGGTCGAGATACAACGACGATGACGTGGCGAATTCCGAATGGGGGTCGGTGAACCTGGAGCACCCGTCGACTTTCGACACCATGGCGATGGACCCGGAGATGAAGAAAATGATCGTGGAGGATTTGGAGAGGTTTGTGAGGAGGAAGGAGTTTTATAAGAAGGTGGGGAAAGCTTGGAAAAGGGGCTACTTGCTGTACGGGCCGCCAGGGACCGGAAAATCGAGCTTGATCGCCGCCATGGCTAACTACCTCAAGTTTGATGTGTACGATTTGGAGCTCACTAGCATTTACAGTGACTCTCAGTTGAAGAAGGTTTTGCTGTCCACTACCAATCGTTCTATTTTGGTAATTGAGGACATTGATTGCTGCAGAGTGGATGTTGGCAACCGGGATTCGGATTCGGATTCGGACTTGGATTCGGATTCGGATTCGGACAGGAGCCGGGTGACACTTTCGGGTCTATTGAACTTTATTGATGGATTGTGGTCAAGCTGTGGAGATGAGAGGATCATTGTGTTTACCACCAACCACAAAGACCGACTGGACCCGGCATTGCTGCGTCCGGGTCGAATGGACCTGCACATTCACATGTCCTATTGCACCACAGCTGGGTACAGGATCTTGGCCTCTAACTACCTTGGCATTCAAGAAAACAACCGCCATCGCCTCTGCGGAGAAATTGAAGGGCTGATTGAGAGCACAGAGGTCACCCCTGCAGAGGTTGCTGAGGAGCTGATGAAGAGTGATGATGCGGATGTGGCTCTTCAAGGACTTGTCAACCTGCTCAAAAGTAAGAAGGCTGAGAGCAAGAAGAAGGAAACATCGCTGCCGTCGGTTAAGAAGCAACAGCGGCACAAAAAGGGGATCAAAAAGTTGCTGCACATCCGTCTTCCATGGTCGAAATCCTCATGCTTGTTGCCTGGCACCTCGCATGTCCTAGTTTAA

Protein Analysis

507

Amino Acids

57.64

Weight (kDa)

8.27

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 40 - 134 1e-23 Domain associated at C-terminal with AAA
AAA PF00004 255 - 390 4.4e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 392 - 465 1.9e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000471)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18190 AT2G18193
fragaria_vesca FvH4_1g12130 FvH4_1g12690 FvH4_1g12700 FvH4_1g12712 FvH4_1g12716 FvH4_1g12731
malus_domestica MD02G1141300.v1.1 MD02G1141400.v1.1 MD02G1141600.v1.1 MD02G1141800.v1.1 MD02G1142200.v1.1 MD15G1254200.v1.1
prunus_persica Prupe.7G159500_v2.0.a1 Prupe.7G159600_v2.0.a1 Prupe.7G165500_v2.0.a1
pyrus_communis pycom02g11130 pycom02g11150 pycom02g11160 pycom02g11180 pycom15g22350
rosa_chinensis RchiOBHm_Chr2g0099791 RchiOBHm_Chr2g0099801 RchiOBHm_Chr2g0100651 RchiOBHm_Chr2g0100681 RchiOBHm_Chr2g0100691 RchiOBHm_Chr2g0100701 RchiOBHm_Chr2g0100721 RchiOBHm_Chr2g0100801 RchiOBHm_Chr3g0459141
rosa_laevigata RLG00000016914 RLG00000016915 RLG00000016997 RLG00000016998 RLG00000017000 RLG00000017002 RLG00000017012 RLG00000025114
rosa_multiflora Rmu_co8416275.1_g000001 Rmu_sc0001187.1_g000004 Rmu_sc0001497.1_g000006 Rmu_sc0001659.1_g000004 Rmu_sc0001659.1_g000006 Rmu_sc0001659.1_g000008 Rmu_sc0001659.1_g000010 Rmu_sc0008864.1_g000008 Rmu_sc0028022.1_g000001
rosa_roxburghii Rroxscaffold_2G00141850 Rroxscaffold_2G00141930 Rroxscaffold_2G00141940 Rroxscaffold_2G00141950 Rroxscaffold_2G00141960 Rroxscaffold_2G00141970
rosa_rugosa Rorug02G0091800 Rorug02G0091800 Rorug02G0091800 Rorug02G0091900 Rorug02G0092900 Rorug03G0033500
rosa_samantha Rh2BG135600 Rh2BG143900 Rh2BG144000 Rh2BG144200 Rh2BG144300 Rh2BG144400 Rh2BG144500 Rh2BG145500 Rh2CG136900 Rh2CG137000 Rh2CG144800 Rh2CG145000 Rh2CG145100 Rh2CG145200 Rh2CG145500 Rh2CG146400 Rh2DG137400 Rh2DG137500 Rh2DG144300 Rh2DG144500 Rh2DG144600 Rh2DG144700 Rh2DG145000 Rh2DG145900 Rh3BG094700 Rh3DG095300
rosa_wichuraiana Rw2G010920 Rw2G010950 Rw2G010990 Rw3G007860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
AasI GACNNNNNNGTC 3 cut(s) 43, 46, 377
Acc36I ACCTGC 4 cut(s) 267, 510, 1161, 1365
AccB1I GGYRCC 2 cut(s) 374, 1499
AccBSI CCGCTC 2 cut(s) 99, 135
AccI GTMKAC 3 cut(s) 63, 237, 644
AccII CGCG 2 cut(s) 245, 297
AclWI GGATC 3 cut(s) 1091, 1202, 1448
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 607
AcyI GRCGYC 3 cut(s) 44, 330, 528
AfaI GTAC 4 cut(s) 500, 773, 842, 1190
AfiI CCNNNNNNNGG 7 cut(s) 417, 444, 618, 788, 970, 1185, 1294
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 5 cut(s) 883, 1042, 1223, 1259, 1343
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 3 cut(s) 630, 781, 1495
Alw21I GWGCWC 6 cut(s) 117, 213, 387, 639, 855, 1277
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 3 cut(s) 1091, 1202, 1448
AoxI GGCC 4 cut(s) 246, 275, 776, 1200
ApaI GGGCCC 1 cut(s) 279
ApeKI GCWGC 4 cut(s) 942, 1132, 1402, 1453
ApoI RAATTY 1 cut(s) 607
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 7 cut(s) 275, 276, 667, 776, 786, 1120, 1150
AsuC2I CCSGG 6 cut(s) 313, 671, 965, 1019, 1124, 1140
AsuHPI GGTGA 6 cut(s) 67, 499, 565, 637, 1033, 1276
AvaII GGWCC 4 cut(s) 667, 786, 1120, 1150
BaeGI GKGCMC 1 cut(s) 279
BanI GGYRCC 2 cut(s) 374, 1499
BanII GRGCYC 5 cut(s) 117, 213, 279, 387, 855
BbsI GAAGAC 1 cut(s) 1457
Bbv12I GWGCWC 6 cut(s) 117, 213, 387, 639, 855, 1277
BbvCI CCTCAGC 1 cut(s) 1302
BbvI GCAGC 4 cut(s) 929, 1119, 1389, 1440
BccI CCATC 4 cut(s) 344, 658, 1046, 1245
BceAI ACGGC 3 cut(s) 57, 362, 1390
BciT130I CCWGG 3 cut(s) 632, 783, 1497
BcnI CCSGG 6 cut(s) 313, 671, 965, 1019, 1124, 1140
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 858, 1514
BfmI CTRYAG 2 cut(s) 943, 1290
BfoI RGCGCY 1 cut(s) 292
BfuAI ACCTGC 4 cut(s) 267, 510, 1161, 1365
BglI GCCNNNNNGGC 1 cut(s) 815
BisI GCNGC 8 cut(s) 246, 779, 810, 943, 1133, 1403, 1427, 1454
BlsI GCNGC 8 cut(s) 247, 780, 811, 944, 1134, 1404, 1428, 1455
Bme1390I CCNGG 9 cut(s) 313, 632, 671, 783, 965, 1019, 1124, 1140, 1497
Bme18I GGWCC 4 cut(s) 667, 786, 1120, 1150
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 7 cut(s) 275, 276, 667, 776, 786, 1120, 1150
BmiI GGNNCC 8 cut(s) 277, 278, 376, 669, 787, 1016, 1122, 1501
BmrFI CCNGG 9 cut(s) 313, 632, 671, 783, 965, 1019, 1124, 1140, 1497
BmsI GCATC 3 cut(s) 21, 78, 1315
BpiI GAAGAC 1 cut(s) 1457
BpmI CTGGAG 1 cut(s) 653
Bpu10I CCTNAGC 2 cut(s) 519, 1302
BpuEI CTTGAG 2 cut(s) 371, 812
BpuMI CCSGG 6 cut(s) 313, 671, 965, 1019, 1124, 1140
BsaHI GRCGYC 3 cut(s) 44, 330, 528
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 7 cut(s) 249, 311, 657, 782, 813, 1213, 1469
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 7 cut(s) 417, 444, 618, 788, 970, 1185, 1294
Bse1I ACTGG 1 cut(s) 1122
Bse3DI GCAATG 1 cut(s) 1127
BseBI CCWGG 3 cut(s) 632, 783, 1497
BseDI CCNNGG 7 cut(s) 249, 311, 657, 782, 813, 1213, 1469
BseGI GGATG 5 cut(s) 117, 434, 958, 1336, 1458
BseLI CCNNNNNNNGG 7 cut(s) 417, 444, 618, 788, 970, 1185, 1294
BseMI GCAATG 1 cut(s) 1127
BseMII CTCAG 4 cut(s) 533, 890, 1293, 1368
BseNI ACTGG 1 cut(s) 1122
BseRI GAGGAG 3 cut(s) 557, 730, 1319
BseSI GKGCMC 1 cut(s) 279
BseX3I CGGCCG 1 cut(s) 246
BseXI GCAGC 4 cut(s) 929, 1119, 1389, 1440
BseYI CCCAGC 1 cut(s) 1184
BsgI GTGCAG 2 cut(s) 1139, 1439
Bsh1236I CGCG 2 cut(s) 245, 297
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 4 cut(s) 248, 277, 778, 1202
BshNI GGYRCC 2 cut(s) 374, 1499
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 6 cut(s) 117, 213, 387, 639, 855, 1277
BsiSI CCGG 8 cut(s) 216, 313, 671, 789, 964, 1018, 1124, 1139
BslFI GGGAC 1 cut(s) 799
BslI CCNNNNNNNGG 7 cut(s) 417, 444, 618, 788, 970, 1185, 1294
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 799
BsmI GAATGC 1 cut(s) 1218
BsnI GGCC 4 cut(s) 248, 277, 778, 1202
Bso31I GGTCTC 1 cut(s) 401
Bsp120I GGGCCC 1 cut(s) 275
Bsp1286I GDGCHC 7 cut(s) 117, 213, 279, 387, 639, 855, 1277
Bsp143I GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
Bsp19I CCATGG 3 cut(s) 657, 813, 1469
BspANI GGCC 4 cut(s) 248, 277, 778, 1202
BspCNI CTCAG 4 cut(s) 532, 889, 1294, 1369
BspFNI CGCG 2 cut(s) 245, 297
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 8 cut(s) 277, 278, 376, 669, 787, 1016, 1122, 1501
BspMAI CTGCAG 2 cut(s) 947, 1294
BspMI ACCTGC 4 cut(s) 267, 510, 1161, 1365
BspPI GGATC 3 cut(s) 1091, 1202, 1448
BspQI GCTCTTC 1 cut(s) 1344
BspT107I GGYRCC 2 cut(s) 374, 1499
BspTNI GGTCTC 1 cut(s) 401
BsrBI CCGCTC 2 cut(s) 99, 135
BsrDI GCAATG 1 cut(s) 1127
BsrI ACTGG 1 cut(s) 1122
BssECI CCNNGG 7 cut(s) 249, 311, 657, 782, 813, 1213, 1469
BssMI GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
BssNI GRCGYC 3 cut(s) 44, 330, 528
BssT1I CCWWGG 4 cut(s) 657, 813, 1213, 1469
Bst2UI CCWGG 3 cut(s) 632, 783, 1497
Bst4CI ACNGT 2 cut(s) 374, 869
Bst6I CTCTTC 3 cut(s) 182, 1310, 1344
BstACI GRCGYC 3 cut(s) 44, 330, 528
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstC8I GCNNGC 2 cut(s) 109, 113
BstDEI CTNAG 4 cut(s) 519, 876, 1302, 1377
BstDSI CCRYGG 3 cut(s) 657, 813, 1469
BstEII GGTNACC 1 cut(s) 1282
BstF5I GGATG 5 cut(s) 117, 434, 958, 1336, 1458
BstFNI CGCG 2 cut(s) 245, 297
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 6 cut(s) 273, 690, 807, 1086, 1197, 1443
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 8 cut(s) 225, 391, 536, 775, 815, 1298, 1334, 1426
BstNI CCWGG 3 cut(s) 632, 783, 1497
BstNSI RCATGY 2 cut(s) 1168, 1511
BstPI GGTNACC 1 cut(s) 1282
BstSCI CCNGG 9 cut(s) 311, 630, 669, 781, 963, 1017, 1122, 1138, 1495
BstSFI CTRYAG 2 cut(s) 943, 1290
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 2 cut(s) 245, 297
BstV1I GCAGC 4 cut(s) 929, 1119, 1389, 1440
BstV2I GAAGAC 1 cut(s) 1457
BstX2I RGATCY 1 cut(s) 1194
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 1 cut(s) 1194
BstZI CGGCCG 1 cut(s) 246
BsuRI GGCC 4 cut(s) 248, 277, 778, 1202
BtgI CCRYGG 3 cut(s) 657, 813, 1469
BtgZI GCGATG 3 cut(s) 677, 1223, 1383
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 5 cut(s) 117, 434, 958, 1336, 1458
BtsIMutI CAGTG 1 cut(s) 874
BveI ACCTGC 4 cut(s) 267, 510, 1161, 1365
Cac8I GCNNGC 2 cut(s) 109, 113
CciI TCATGA 1 cut(s) 84
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 7 cut(s) 275, 276, 667, 776, 786, 1120, 1150
CseI GACGC 6 cut(s) 33, 251, 286, 319, 536, 1124
Csp6I GTAC 4 cut(s) 499, 772, 841, 1189
CviAII CATG 9 cut(s) 85, 220, 478, 658, 814, 1165, 1470, 1485, 1508
CviQI GTAC 4 cut(s) 499, 772, 841, 1189
DdeI CTNAG 4 cut(s) 519, 876, 1302, 1377
DpnI GATC 6 cut(s) 272, 689, 806, 1085, 1196, 1442
DpnII GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
DrdI GACNNNNNNGTC 3 cut(s) 43, 46, 377
DseDI GACNNNNNNGTC 3 cut(s) 43, 46, 377
EaeI YGGCCR 1 cut(s) 246
EagI CGGCCG 1 cut(s) 246
Eam1104I CTCTTC 3 cut(s) 182, 1310, 1344
EarI CTCTTC 3 cut(s) 182, 1310, 1344
Ecl136II GAGCTC 4 cut(s) 115, 211, 385, 853
EclXI CGGCCG 1 cut(s) 246
Eco130I CCWWGG 4 cut(s) 657, 813, 1213, 1469
Eco24I GRGCYC 5 cut(s) 117, 213, 279, 387, 855
Eco31I GGTCTC 1 cut(s) 401
Eco47I GGWCC 4 cut(s) 667, 786, 1120, 1150
Eco52I CGGCCG 1 cut(s) 246
Eco53kI GAGCTC 4 cut(s) 115, 211, 385, 853
Eco91I GGTNACC 1 cut(s) 1282
EcoICRI GAGCTC 4 cut(s) 115, 211, 385, 853
EcoO109I RGGNCCY 1 cut(s) 276
EcoO65I GGTNACC 1 cut(s) 1282
EcoRI GAATTC 1 cut(s) 607
EcoRII CCWGG 3 cut(s) 630, 781, 1495
EcoT14I CCWWGG 4 cut(s) 657, 813, 1213, 1469
EcoT38I GRGCYC 5 cut(s) 117, 213, 279, 387, 855
ErhI CCWWGG 4 cut(s) 657, 813, 1213, 1469
FaeI CATG 9 cut(s) 88, 223, 481, 661, 817, 1168, 1473, 1488, 1511
FaqI GGGAC 1 cut(s) 799
FatI CATG 9 cut(s) 84, 219, 477, 657, 813, 1164, 1469, 1484, 1507
FauI CCCGC 3 cut(s) 130, 233, 575
FblI GTMKAC 3 cut(s) 63, 237, 644
Fnu4HI GCNGC 8 cut(s) 246, 779, 810, 943, 1133, 1403, 1427, 1454
FokI GGATG 5 cut(s) 104, 421, 965, 1343, 1445
FriOI GRGCYC 5 cut(s) 117, 213, 279, 387, 855
Fsp4HI GCNGC 8 cut(s) 246, 779, 810, 943, 1133, 1403, 1427, 1454
FspBI CTAG 2 cut(s) 858, 1514
GlaI GCGC 1 cut(s) 290
GluI GCNGC 8 cut(s) 246, 779, 810, 943, 1133, 1403, 1427, 1454
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 653
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 4 cut(s) 248, 277, 778, 1202
HapII CCGG 8 cut(s) 216, 313, 671, 789, 964, 1018, 1124, 1139
HgaI GACGC 6 cut(s) 33, 251, 286, 319, 536, 1124
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 3 cut(s) 44, 330, 528
Hin1II CATG 9 cut(s) 88, 223, 481, 661, 817, 1168, 1473, 1488, 1511
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 2 cut(s) 645, 1354
HindII GTYRAC 2 cut(s) 645, 1354
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 7 cut(s) 872, 968, 974, 980, 992, 998, 1004
HpaII CCGG 8 cut(s) 216, 313, 671, 789, 964, 1018, 1124, 1139
HphI GGTGA 6 cut(s) 67, 499, 565, 637, 1033, 1276
Hpy166II GTNNAC 9 cut(s) 64, 238, 256, 628, 645, 841, 900, 1095, 1354
Hpy188III TCNNGA 4 cut(s) 11, 85, 582, 1223
Hpy8I GTNNAC 9 cut(s) 64, 238, 256, 628, 645, 841, 900, 1095, 1354
HpyCH4III ACNGT 2 cut(s) 374, 869
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 5 cut(s) 945, 1156, 1176, 1292, 1456
HpyF10VI GCNNNNNNNGC 8 cut(s) 225, 391, 536, 775, 815, 1298, 1334, 1426
HpyF3I CTNAG 4 cut(s) 519, 876, 1302, 1377
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 3 cut(s) 44, 330, 528
Hsp92II CATG 9 cut(s) 88, 223, 481, 661, 817, 1168, 1473, 1488, 1511
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
LguI GCTCTTC 1 cut(s) 1344
LmnI GCTCC 7 cut(s) 104, 140, 313, 634, 850, 1014, 1306
Lsp1109I GCAGC 4 cut(s) 929, 1119, 1389, 1440
LweI GCATC 3 cut(s) 21, 78, 1315
MaeI CTAG 2 cut(s) 858, 1514
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 5 cut(s) 73, 185, 869, 1021, 1282
MalI GATC 6 cut(s) 272, 689, 806, 1085, 1196, 1442
MbiI CCGCTC 2 cut(s) 99, 135
MboI GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
MflI RGATCY 1 cut(s) 1194
MhlI GDGCHC 7 cut(s) 117, 213, 279, 387, 639, 855, 1277
MluCI AATT 4 cut(s) 157, 607, 924, 1254
MlyI GAGTC 1 cut(s) 866
MmeI TCCRAC 1 cut(s) 384
MseI TTAA 3 cut(s) 534, 1413, 1519
MslI CAYNNNNRTG 3 cut(s) 83, 662, 1163
MspA1I CMGCKG 2 cut(s) 1184, 1426
MspI CCGG 8 cut(s) 216, 313, 671, 789, 964, 1018, 1124, 1139
MspR9I CCNGG 9 cut(s) 313, 632, 671, 783, 965, 1019, 1124, 1140, 1497
Mva1269I GAATGC 1 cut(s) 1218
MvaI CCWGG 3 cut(s) 632, 783, 1497
MvnI CGCG 2 cut(s) 245, 297
MwoI GCNNNNNNNGC 8 cut(s) 225, 391, 536, 775, 815, 1298, 1334, 1426
NciI CCSGG 6 cut(s) 313, 671, 965, 1019, 1124, 1140
NcoI CCATGG 3 cut(s) 657, 813, 1469
NdeII GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
NlaIII CATG 9 cut(s) 88, 223, 481, 661, 817, 1168, 1473, 1488, 1511
NlaIV GGNNCC 8 cut(s) 277, 278, 376, 669, 787, 1016, 1122, 1501
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 5 cut(s) 73, 185, 869, 1021, 1282
NspI RCATGY 2 cut(s) 1168, 1511
PagI TCATGA 1 cut(s) 84
PciI ACATGT 1 cut(s) 1164
PciSI GCTCTTC 1 cut(s) 1344
PcsI WCGNNNNNNNCGW 7 cut(s) 44, 47, 246, 345, 375, 378, 597
PctI GAATGC 1 cut(s) 1218
PfeI GAWTC 6 cut(s) 968, 974, 980, 992, 998, 1004
PflFI GACNNNGTC 1 cut(s) 43
PkrI GCNGC 8 cut(s) 247, 780, 811, 944, 1134, 1404, 1428, 1455
PleI GAGTC 1 cut(s) 866
PpsI GAGTC 1 cut(s) 866
PscI ACATGT 1 cut(s) 1164
PsiI TTATAA 1 cut(s) 732
Psp124BI GAGCTC 4 cut(s) 117, 213, 387, 855
Psp6I CCWGG 3 cut(s) 630, 781, 1495
PspEI GGTNACC 1 cut(s) 1282
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 3 cut(s) 630, 781, 1495
PspN4I GGNNCC 8 cut(s) 277, 278, 376, 669, 787, 1016, 1122, 1501
PspOMI GGGCCC 1 cut(s) 275
PspPI GGNCC 7 cut(s) 275, 276, 667, 776, 786, 1120, 1150
PstI CTGCAG 2 cut(s) 947, 1294
PsuI RGATCY 1 cut(s) 1194
PsyI GACNNNGTC 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 4 cut(s) 500, 773, 842, 1190
RsaNI GTAC 4 cut(s) 499, 772, 841, 1189
RseI CAYNNNNRTG 3 cut(s) 83, 662, 1163
SacI GAGCTC 4 cut(s) 117, 213, 387, 855
SalI GTCGAC 1 cut(s) 643
SapI GCTCTTC 1 cut(s) 1344
SaqAI TTAA 3 cut(s) 534, 1413, 1519
SatI GCNGC 8 cut(s) 246, 779, 810, 943, 1133, 1403, 1427, 1454
Sau3AI GATC 6 cut(s) 270, 687, 804, 1083, 1194, 1440
Sau96I GGNCC 7 cut(s) 275, 276, 667, 776, 786, 1120, 1150
SchI GAGTC 1 cut(s) 866
ScrFI CCNGG 9 cut(s) 313, 632, 671, 783, 965, 1019, 1124, 1140, 1497
SduI GDGCHC 7 cut(s) 117, 213, 279, 387, 639, 855, 1277
SfaNI GCATC 3 cut(s) 21, 78, 1315
SfcI CTRYAG 2 cut(s) 943, 1290
SinI GGWCC 4 cut(s) 667, 786, 1120, 1150
SmiMI CAYNNNNRTG 3 cut(s) 83, 662, 1163
SmlI CTYRAG 2 cut(s) 386, 827
SmoI CTYRAG 2 cut(s) 386, 827
Sse9I AATT 4 cut(s) 157, 607, 924, 1254
SspMI CTAG 2 cut(s) 858, 1514
SstI GAGCTC 4 cut(s) 117, 213, 387, 855
StyD4I CCNGG 9 cut(s) 311, 630, 669, 781, 963, 1017, 1122, 1138, 1495
StyI CCWWGG 4 cut(s) 657, 813, 1213, 1469
TaaI ACNGT 2 cut(s) 374, 869
TaiI ACGT 1 cut(s) 603
TaqII GACCGA 2 cut(s) 425, 1128
TasI AATT 4 cut(s) 157, 607, 924, 1254
TauI GCSGC 4 cut(s) 248, 781, 812, 1429
TfiI GAWTC 6 cut(s) 968, 974, 980, 992, 998, 1004
Tru1I TTAA 3 cut(s) 534, 1413, 1519
Tru9I TTAA 3 cut(s) 534, 1413, 1519
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 5 cut(s) 73, 185, 869, 1021, 1282
TseI GCWGC 4 cut(s) 942, 1132, 1402, 1453
Tsp45I GTSAC 5 cut(s) 73, 185, 869, 1021, 1282
TspDTI ATGAA 3 cut(s) 73, 692, 1328
TspGWI ACGGA 4 cut(s) 49, 61, 91, 1451
TspRI CASTG 1 cut(s) 874
Tth111I GACNNNGTC 1 cut(s) 43
VpaK11BI GGWCC 4 cut(s) 667, 786, 1120, 1150
XapI RAATTY 1 cut(s) 607
XceI RCATGY 2 cut(s) 1168, 1511
XmiI GTMKAC 3 cut(s) 63, 237, 644
XspI CTAG 2 cut(s) 858, 1514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.