Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000471

Physical Location & Seq

Genomic Coordinates Reverse (-)

11771695 .. 11773185

Gene Structure 1491 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1141600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGTTTACATCTCTCAATCTCAAAGAAATGCCAACAAACGCGTCCTCCTTGTTCTCAGCCTATGCCTCCTTCGCTGCATTCATGATGTTGGTCCGCACCGCGGCCAACGAACTCATGAACCTTGTACCCGACAGGGTCCGGGCGTACATAGAATCGGTCCTTCTCCATCTAGTCACCCCTCTCTCGGCTTCCCATGATCTAACTCTGGTCATTGACGAGAACGCCGGCATGACAAGAAACCAAATCTATGAGGCCGCGGACGTCTACCTTCAAACCAAGATCAGTCCCTTAAACGAACGTCTTCGGGTCAGCAAAACACCACGACAGAAAACCATCAGCCTCGCCATCGAAAAAGGCCAAGAAGTCATCGATACCTTCGATAACATTGTCCTAAAATGGCGTTACGTCTGCATTGAATCCGATAATAATCCTGGTCGTGATCAAAAGCGTCAATTCGAGGTAATATTCCACAAGAGGCACAAGGACAAAGTTGTCCTCTCCTACTTGCCTTACGTGCTTGATCGAGCCAATGCAATTAAAGAACAAGACAGAGTTGTGAAAATTTATACACGAAATTCATATCCGAACTACGATGATGATGACAATCATGATTGGGGTTCGATAAGTCTGGAGCACCCGGCTACGTTTGAAACAATGGCAATGGACCCCGAGCTTAAGACCATGATGAAAAATGATTTGGACAGGTTTGTGAGACGGAGGGAGTTCTATAAGAAGGTGGGGAAGGCTTGGAAAAGAGGCTACTTGCTGTACGGTCCTCCCGGCACTGGAAAATCTAGCTTGATCGCGGCCATGGCAAATTATCTTCATTTCAACATTTATGATTTGGAGCTTTCTAGTATTTACAGAGACTCGGAATTGAAGAGGGTATTGTTGTCTACTACAAATCGTTCGATTTTAGTAATTGAGGACATTGATTGCAGCATTGATGTCGAAAATCGTGAATCGGAAGAGCAGCAACAATCATCTCAAACCAAATTGACGCTTTCGGGCCTATTGAACTTTATTGATGGTTTGTGGTCGAGCTGCGGTGACGAGAGAATTATCGTGTTCACTACCAACCACAAGGATCGATTAGACCCTGCATTGTTGCGTCCAGGACGAATGGACGTGCACATTCACTTATCGTACTGCACTGCAAGTGGATTCAAGGTCTTGGCCTCGAACTACCTTGGCATTGGTGACGAAAACCATCATCGGCTTTATGGGGAAATTGAAGGGTTGATAGAGAGTGCGGAGGTGACCCCTGCAGAGGTTGCTGAGGAGCTCATGAAGAGCGATGACGCTGATGTGGTGCTCGTAGGACTTGTTGATTTCCTCAAATGTAAGAAGGCCGAGAGGCAACGAAAAAAGGAGGAAGAGAGTAACCAAATTAAAGATCAGGAAGCAGCAATGAAGAAAACGGATGTTGAGGACAATATTAACGACGAAGGCAAGGGCTCGCTGCATTCGAAAACCTTGAATATGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006996 GO:0007005 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017004 GO:0017062 GO:0017111 GO:0019866 GO:0022607 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033108 GO:0034551 GO:0034622 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0065003 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA AAA_assoc

Protein Analysis

497

Amino Acids

56.7

Weight (kDa)

5.65

Isoelectric Point (pI)

41.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_assoc	PF14363	42 - 137	4.2e-22	Domain associated at C-terminal with AAA
AAA	PF00004	254 - 382	2e-21	ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540	PF25568	384 - 457	8.6e-23	At3g28540-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000471)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18190 AT2G18193
fragaria_vesca	FvH4_1g12130 FvH4_1g12690 FvH4_1g12700 FvH4_1g12712 FvH4_1g12716 FvH4_1g12731
malus_domestica	MD02G1141300.v1.1 MD02G1141400.v1.1 MD02G1141600.v1.1 MD02G1141800.v1.1 MD02G1142200.v1.1 MD15G1254200.v1.1
prunus_persica	Prupe.7G159500_v2.0.a1 Prupe.7G159600_v2.0.a1 Prupe.7G165500_v2.0.a1
pyrus_communis	pycom02g11130 pycom02g11150 pycom02g11160 pycom02g11180 pycom15g22350
rosa_chinensis	RchiOBHm_Chr2g0099791 RchiOBHm_Chr2g0099801 RchiOBHm_Chr2g0100651 RchiOBHm_Chr2g0100681 RchiOBHm_Chr2g0100691 RchiOBHm_Chr2g0100701 RchiOBHm_Chr2g0100721 RchiOBHm_Chr2g0100801 RchiOBHm_Chr3g0459141
rosa_laevigata	RLG00000016914 RLG00000016915 RLG00000016997 RLG00000016998 RLG00000017000 RLG00000017002 RLG00000017012 RLG00000025114
rosa_multiflora	Rmu_co8416275.1_g000001 Rmu_sc0001187.1_g000004 Rmu_sc0001497.1_g000006 Rmu_sc0001659.1_g000004 Rmu_sc0001659.1_g000006 Rmu_sc0001659.1_g000008 Rmu_sc0001659.1_g000010 Rmu_sc0008864.1_g000008 Rmu_sc0028022.1_g000001
rosa_roxburghii	Rroxscaffold_2G00141850 Rroxscaffold_2G00141930 Rroxscaffold_2G00141940 Rroxscaffold_2G00141950 Rroxscaffold_2G00141960 Rroxscaffold_2G00141970
rosa_rugosa	Rorug02G0091800 Rorug02G0091800 Rorug02G0091800 Rorug02G0091900 Rorug02G0092900 Rorug03G0033500
rosa_samantha	Rh2BG135600 Rh2BG143900 Rh2BG144000 Rh2BG144200 Rh2BG144300 Rh2BG144400 Rh2BG144500 Rh2BG145500 Rh2CG136900 Rh2CG137000 Rh2CG144800 Rh2CG145000 Rh2CG145100 Rh2CG145200 Rh2CG145500 Rh2CG146400 Rh2DG137400 Rh2DG137500 Rh2DG144300 Rh2DG144500 Rh2DG144600 Rh2DG144700 Rh2DG145000 Rh2DG145900 Rh3BG094700 Rh3DG095300
rosa_wichuraiana	Rw2G010920 Rw2G010950 Rw2G010990 Rw3G007860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	491
AatII	GACGTC	1 cut(s)	264
AccI	GTMKAC	2 cut(s)	264, 896
AccII	CGCG	4 cut(s)	41, 101, 257, 806
AciI	CCGC	8 cut(s)	94, 99, 101, 255, 257, 806, 1047, 1253
AclWI	GGATC	1 cut(s)	1095
AcoI	YGGCCR	2 cut(s)	102, 807
AcsI	RAATTY	2 cut(s)	561, 574
AcyI	GRCGYC	1 cut(s)	261
AfaI	GTAC	4 cut(s)	126, 146, 770, 1148
AfiI	CCNNNNNNNGG	4 cut(s)	100, 184, 785, 1270
AflII	CTTAAG	1 cut(s)	674
AflIII	ACRYGT	1 cut(s)	39
AgsI	TTSAA	9 cut(s)	272, 416, 650, 832, 880, 1018, 1168, 1235, 1480
AjiI	CACGTC	1 cut(s)	1129
AjnI	CCWGG	2 cut(s)	430, 1114
AjuI	GAANNNNNNNTTGG	2 cut(s)	680, 712
AluBI	AGCT	5 cut(s)	673, 798, 850, 1044, 1285
AluI	AGCT	5 cut(s)	673, 798, 850, 1044, 1285
Alw21I	GWGCWC	4 cut(s)	636, 1134, 1287, 1317
Alw26I	GTCTC	2 cut(s)	706, 861
Alw44I	GTGCAC	1 cut(s)	1130
AlwI	GGATC	1 cut(s)	1095
Ama87I	CYCGRG	1 cut(s)	668
AoxI	GGCC	7 cut(s)	102, 252, 355, 807, 1009, 1176, 1350
ApaLI	GTGCAC	1 cut(s)	1130
ApeKI	GCWGC	6 cut(s)	74, 939, 973, 1044, 1406, 1462
ApoI	RAATTY	2 cut(s)	561, 574
Asp700I	GAANNNNTTC	1 cut(s)	300
AspS9I	GGNCC	6 cut(s)	91, 136, 157, 664, 773, 1009
AsuC2I	CCSGG	3 cut(s)	140, 638, 780
AsuHPI	GGTGA	4 cut(s)	166, 1061, 1211, 1270
AsuII	TTCGAA	1 cut(s)	1469
AvaI	CYCGRG	1 cut(s)	668
AvaII	GGWCC	5 cut(s)	91, 136, 157, 664, 773
BaeGI	GKGCMC	1 cut(s)	1134
BanII	GRGCYC	2 cut(s)	1287, 1460
BbsI	GAAGAC	1 cut(s)	293
Bbv12I	GWGCWC	4 cut(s)	636, 1134, 1287, 1317
BbvCI	CCTCAGC	1 cut(s)	1278
BbvI	GCAGC	6 cut(s)	61, 951, 985, 1031, 1418, 1449
BccI	CCATC	5 cut(s)	174, 341, 353, 1022, 1218
BcgI	CGANNNNNNTGC	2 cut(s)	931, 965
BciT130I	CCWGG	2 cut(s)	432, 1116
BclI	TGATCA	1 cut(s)	439
BcnI	CCSGG	3 cut(s)	140, 638, 780
BcoDI	GTCTC	2 cut(s)	706, 861
BfaI	CTAG	3 cut(s)	170, 795, 855
BfmI	CTRYAG	1 cut(s)	1266
BfrI	CTTAAG	1 cut(s)	674
BisI	GCNGC	9 cut(s)	75, 102, 255, 807, 940, 974, 1045, 1407, 1463
BlsI	GCNGC	9 cut(s)	76, 103, 256, 808, 941, 975, 1046, 1408, 1464
Bme1390I	CCNGG	5 cut(s)	140, 432, 638, 780, 1116
Bme18I	GGWCC	5 cut(s)	91, 136, 157, 664, 773
BmeT110I	CYCGRG	1 cut(s)	668
BmgBI	CACGTC	1 cut(s)	1129
BmgT120I	GGNCC	6 cut(s)	91, 136, 157, 664, 773, 1009
BmiI	GGNNCC	2 cut(s)	137, 666
BmrFI	CCNGG	5 cut(s)	140, 432, 638, 780, 1116
BpiI	GAAGAC	1 cut(s)	293
BpmI	CTGGAG	1 cut(s)	650
Bpu10I	CCTNAGC	1 cut(s)	1278
Bpu14I	TTCGAA	1 cut(s)	1469
BpuMI	CCSGG	3 cut(s)	140, 638, 780
Bsa29I	ATCGAT	2 cut(s)	369, 1090
BsaAI	YACGTR	1 cut(s)	514
BsaBI	GATNNNNATC	2 cut(s)	426, 603
BsaHI	GRCGYC	1 cut(s)	261
BsaJI	CCNNGG	4 cut(s)	99, 255, 810, 1189
BsaXI	ACNNNNNCTCC	2 cut(s)	29, 59
Bsc4I	CCNNNNNNNGG	4 cut(s)	100, 184, 785, 1270
Bse118I	RCCGGY	1 cut(s)	224
Bse1I	ACTGG	1 cut(s)	790
Bse3DI	GCAATG	2 cut(s)	666, 1416
Bse8I	GATNNNNATC	2 cut(s)	426, 603
BseBI	CCWGG	2 cut(s)	432, 1116
BseCI	ATCGAT	2 cut(s)	369, 1090
BseDI	CCNNGG	4 cut(s)	99, 255, 810, 1189
BseGI	GGATG	1 cut(s)	1429
BseJI	GATNNNNATC	2 cut(s)	426, 603
BseLI	CCNNNNNNNGG	4 cut(s)	100, 184, 785, 1270
BseMI	GCAATG	2 cut(s)	666, 1416
BseMII	CTCAG	2 cut(s)	69, 1269
BseNI	ACTGG	1 cut(s)	790
BseRI	GAGGAG	1 cut(s)	1295
BseSI	GKGCMC	1 cut(s)	1134
BseXI	GCAGC	6 cut(s)	61, 951, 985, 1031, 1418, 1449
BsgI	GTGCAG	1 cut(s)	1135
Bsh1236I	CGCG	4 cut(s)	41, 101, 257, 806
BshFI	GGCC	7 cut(s)	104, 254, 357, 809, 1011, 1178, 1352
BshVI	ATCGAT	2 cut(s)	369, 1090
BsiHKAI	GWGCWC	4 cut(s)	636, 1134, 1287, 1317
BsiHKCI	CYCGRG	1 cut(s)	668
BsiSI	CCGG	4 cut(s)	139, 225, 638, 780
BslFI	GGGAC	1 cut(s)	270
BslI	CCNNNNNNNGG	4 cut(s)	100, 184, 785, 1270
BsmAI	GTCTC	2 cut(s)	706, 861
BsmBI	CGTCTC	1 cut(s)	706
BsmFI	GGGAC	1 cut(s)	270
BsmI	GAATGC	2 cut(s)	77, 1465
BsnI	GGCC	7 cut(s)	104, 254, 357, 809, 1011, 1178, 1352
BsoBI	CYCGRG	1 cut(s)	668
Bsp119I	TTCGAA	1 cut(s)	1469
Bsp1286I	GDGCHC	5 cut(s)	636, 1134, 1287, 1317, 1460
Bsp143I	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
Bsp19I	CCATGG	1 cut(s)	810
BspACI	CCGC	8 cut(s)	94, 99, 101, 255, 257, 806, 1047, 1253
BspANI	GGCC	7 cut(s)	104, 254, 357, 809, 1011, 1178, 1352
BspCNI	CTCAG	2 cut(s)	68, 1270
BspDI	ATCGAT	2 cut(s)	369, 1090
BspFNI	CGCG	4 cut(s)	41, 101, 257, 806
BspHI	TCATGA	4 cut(s)	81, 114, 607, 1287
BspLI	GGNNCC	2 cut(s)	137, 666
BspMAI	CTGCAG	1 cut(s)	1270
BspPI	GGATC	1 cut(s)	1095
BspQI	GCTCTTC	2 cut(s)	963, 1286
BspT104I	TTCGAA	1 cut(s)	1469
BspTI	CTTAAG	1 cut(s)	674
BsrDI	GCAATG	2 cut(s)	666, 1416
BsrFI	RCCGGY	1 cut(s)	224
BsrI	ACTGG	1 cut(s)	790
BssAI	RCCGGY	1 cut(s)	224
BssECI	CCNNGG	4 cut(s)	99, 255, 810, 1189
BssMI	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
BssNI	GRCGYC	1 cut(s)	261
BssT1I	CCWWGG	2 cut(s)	810, 1189
Bst2UI	CCWGG	2 cut(s)	432, 1116
Bst4CI	ACNGT	1 cut(s)	773
Bst6I	CTCTTC	4 cut(s)	875, 963, 1286, 1371
BstACI	GRCGYC	1 cut(s)	261
BstAFI	CTTAAG	1 cut(s)	674
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstBAI	YACGTR	1 cut(s)	514
BstBI	TTCGAA	1 cut(s)	1469
BstC8I	GCNNGC	2 cut(s)	226, 1460
BstDEI	CTNAG	2 cut(s)	55, 1278
BstDSI	CCRYGG	3 cut(s)	99, 255, 810
BstEII	GGTNACC	1 cut(s)	1258
BstF5I	GGATG	1 cut(s)	1429
BstFNI	CGCG	4 cut(s)	41, 101, 257, 806
BstKTI	GATC	7 cut(s)	199, 282, 442, 523, 804, 1090, 1399
BstMAI	GTCTC	2 cut(s)	706, 861
BstMBI	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
BstMWI	GCNNNNNNNGC	4 cut(s)	71, 514, 812, 1274
BstNI	CCWGG	2 cut(s)	432, 1116
BstPI	GGTNACC	1 cut(s)	1258
BstSCI	CCNGG	5 cut(s)	138, 430, 636, 778, 1114
BstSFI	CTRYAG	1 cut(s)	1266
BstSLI	GKGCMC	1 cut(s)	1134
BstUI	CGCG	4 cut(s)	41, 101, 257, 806
BstV1I	GCAGC	6 cut(s)	61, 951, 985, 1031, 1418, 1449
BstV2I	GAAGAC	1 cut(s)	293
Bsu15I	ATCGAT	2 cut(s)	369, 1090
BsuRI	GGCC	7 cut(s)	104, 254, 357, 809, 1011, 1178, 1352
BsuTUI	ATCGAT	2 cut(s)	369, 1090
BtgI	CCRYGG	3 cut(s)	99, 255, 810
BtgZI	GCGATG	1 cut(s)	1311
BtrI	CACGTC	1 cut(s)	1129
BtsCI	GGATG	1 cut(s)	1429
BtsI	GCAGTG	1 cut(s)	1152
BtsIMutI	CAGTG	2 cut(s)	783, 1152
Cac8I	GCNNGC	2 cut(s)	226, 1460
CciI	TCATGA	4 cut(s)	81, 114, 607, 1287
Cfr10I	RCCGGY	1 cut(s)	224
Cfr13I	GGNCC	6 cut(s)	91, 136, 157, 664, 773, 1009
Cfr42I	CCGCGG	2 cut(s)	102, 258
ClaI	ATCGAT	2 cut(s)	369, 1090
CseI	GACGC	5 cut(s)	30, 437, 1009, 1100, 1310
Csp6I	GTAC	4 cut(s)	125, 145, 769, 1147
CviAII	CATG	8 cut(s)	82, 115, 194, 229, 608, 682, 811, 1288
CviQI	GTAC	4 cut(s)	125, 145, 769, 1147
DdeI	CTNAG	2 cut(s)	55, 1278
DpnI	GATC	7 cut(s)	198, 281, 441, 522, 803, 1089, 1398
DpnII	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
DrdI	GACNNNNNNGTC	1 cut(s)	491
DseDI	GACNNNNNNGTC	1 cut(s)	491
EaeI	YGGCCR	2 cut(s)	102, 807
Eam1104I	CTCTTC	4 cut(s)	875, 963, 1286, 1371
EarI	CTCTTC	4 cut(s)	875, 963, 1286, 1371
Ecl136II	GAGCTC	1 cut(s)	1285
Eco130I	CCWWGG	2 cut(s)	810, 1189
Eco24I	GRGCYC	2 cut(s)	1287, 1460
Eco47I	GGWCC	5 cut(s)	91, 136, 157, 664, 773
Eco53kI	GAGCTC	1 cut(s)	1285
Eco88I	CYCGRG	1 cut(s)	668
Eco91I	GGTNACC	1 cut(s)	1258
EcoICRI	GAGCTC	1 cut(s)	1285
EcoO65I	GGTNACC	1 cut(s)	1258
EcoRII	CCWGG	2 cut(s)	430, 1114
EcoT14I	CCWWGG	2 cut(s)	810, 1189
EcoT38I	GRGCYC	2 cut(s)	1287, 1460
ErhI	CCWWGG	2 cut(s)	810, 1189
Esp3I	CGTCTC	1 cut(s)	706
FaeI	CATG	8 cut(s)	85, 118, 197, 232, 611, 685, 814, 1291
FaqI	GGGAC	1 cut(s)	270
FatI	CATG	8 cut(s)	81, 114, 193, 228, 607, 681, 810, 1287
FbaI	TGATCA	1 cut(s)	439
FblI	GTMKAC	2 cut(s)	264, 896
Fnu4HI	GCNGC	9 cut(s)	75, 102, 255, 807, 940, 974, 1045, 1407, 1463
FokI	GGATG	1 cut(s)	1436
FriOI	GRGCYC	2 cut(s)	1287, 1460
Fsp4HI	GCNGC	9 cut(s)	75, 102, 255, 807, 940, 974, 1045, 1407, 1463
FspBI	CTAG	3 cut(s)	170, 795, 855
GluI	GCNGC	9 cut(s)	75, 102, 255, 807, 940, 974, 1045, 1407, 1463
GsuI	CTGGAG	1 cut(s)	650
HaeIII	GGCC	7 cut(s)	104, 254, 357, 809, 1011, 1178, 1352
HapII	CCGG	4 cut(s)	139, 225, 638, 780
HgaI	GACGC	5 cut(s)	30, 437, 1009, 1100, 1310
Hin1I	GRCGYC	1 cut(s)	261
Hin1II	CATG	8 cut(s)	85, 118, 197, 232, 611, 685, 814, 1291
HinfI	GANTC	5 cut(s)	152, 416, 869, 962, 1164
HpaII	CCGG	4 cut(s)	139, 225, 638, 780
HphI	GGTGA	4 cut(s)	166, 1061, 1211, 1270
Hpy166II	GTNNAC	5 cut(s)	6, 265, 897, 1071, 1132
Hpy188I	TCNGA	4 cut(s)	421, 585, 874, 967
Hpy188III	TCNNGA	8 cut(s)	82, 115, 437, 608, 629, 959, 1288, 1400
Hpy8I	GTNNAC	5 cut(s)	6, 265, 897, 1071, 1132
Hpy99I	CGWCG	1 cut(s)	1448
HpyAV	CCTTC	9 cut(s)	79, 170, 278, 385, 727, 736, 1229, 1342, 1442
HpyCH4III	ACNGT	1 cut(s)	773
HpyCH4IV	ACGT	6 cut(s)	261, 298, 405, 513, 644, 1128
HpyF10VI	GCNNNNNNNGC	4 cut(s)	71, 514, 812, 1274
HpyF3I	CTNAG	2 cut(s)	55, 1278
HpySE526I	ACGT	6 cut(s)	261, 298, 405, 513, 644, 1128
Hsp92I	GRCGYC	1 cut(s)	261
Hsp92II	CATG	8 cut(s)	85, 118, 197, 232, 611, 685, 814, 1291
KroI	GCCGGC	1 cut(s)	224
KroNI	GCCGGC	1 cut(s)	226
Ksp22I	TGATCA	1 cut(s)	439
KspI	CCGCGG	2 cut(s)	102, 258
Kzo9I	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
LguI	GCTCTTC	2 cut(s)	963, 1286
LmnI	GCTCC	3 cut(s)	631, 847, 1282
Lsp1109I	GCAGC	6 cut(s)	61, 951, 985, 1031, 1418, 1449
MaeI	CTAG	3 cut(s)	170, 795, 855
MaeII	ACGT	6 cut(s)	261, 298, 405, 513, 644, 1128
MaeIII	GTNAC	6 cut(s)	172, 401, 1049, 1199, 1258, 1382
MalI	GATC	7 cut(s)	198, 281, 441, 522, 803, 1089, 1398
MboI	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
MboII	GAAGA	7 cut(s)	293, 815, 892, 980, 1303, 1388, 1426
MhlI	GDGCHC	5 cut(s)	636, 1134, 1287, 1317, 1460
MluI	ACGCGT	1 cut(s)	39
MlyI	GAGTC	1 cut(s)	863
MroNI	GCCGGC	1 cut(s)	224
MroXI	GAANNNNTTC	1 cut(s)	300
MseI	TTAA	5 cut(s)	290, 537, 675, 1392, 1440
MspA1I	CMGCKG	2 cut(s)	101, 257
MspCI	CTTAAG	1 cut(s)	674
MspI	CCGG	4 cut(s)	139, 225, 638, 780
MspR9I	CCNGG	5 cut(s)	140, 432, 638, 780, 1116
Mva1269I	GAATGC	2 cut(s)	77, 1465
MvaI	CCWGG	2 cut(s)	432, 1116
MvnI	CGCG	4 cut(s)	41, 101, 257, 806
MwoI	GCNNNNNNNGC	4 cut(s)	71, 514, 812, 1274
NaeI	GCCGGC	1 cut(s)	226
NciI	CCSGG	3 cut(s)	140, 638, 780
NcoI	CCATGG	1 cut(s)	810
NdeII	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
NgoMIV	GCCGGC	1 cut(s)	224
NlaIII	CATG	8 cut(s)	85, 118, 197, 232, 611, 685, 814, 1291
NlaIV	GGNNCC	2 cut(s)	137, 666
NmeAIII	GCCGAG	2 cut(s)	164, 1378
NmuCI	GTSAC	4 cut(s)	172, 1049, 1199, 1258
NspV	TTCGAA	1 cut(s)	1469
PagI	TCATGA	4 cut(s)	81, 114, 607, 1287
PciSI	GCTCTTC	2 cut(s)	963, 1286
PcsI	WCGNNNNNNNCGW	2 cut(s)	375, 1466
PctI	GAATGC	2 cut(s)	77, 1465
PdiI	GCCGGC	1 cut(s)	226
PdmI	GAANNNNTTC	1 cut(s)	300
PfeI	GAWTC	4 cut(s)	152, 416, 962, 1164
PflFI	GACNNNGTC	1 cut(s)	134
PfoI	TCCNGGA	1 cut(s)	1114
PkrI	GCNGC	9 cut(s)	76, 103, 256, 808, 941, 975, 1046, 1408, 1464
PleI	GAGTC	1 cut(s)	863
PpsI	GAGTC	1 cut(s)	863
Ppu21I	YACGTR	1 cut(s)	514
Psp124BI	GAGCTC	1 cut(s)	1287
Psp6I	CCWGG	2 cut(s)	430, 1114
PspEI	GGTNACC	1 cut(s)	1258
PspGI	CCWGG	2 cut(s)	430, 1114
PspN4I	GGNNCC	2 cut(s)	137, 666
PspPI	GGNCC	6 cut(s)	91, 136, 157, 664, 773, 1009
PstI	CTGCAG	1 cut(s)	1270
PsyI	GACNNNGTC	1 cut(s)	134
RsaI	GTAC	4 cut(s)	126, 146, 770, 1148
RsaNI	GTAC	4 cut(s)	125, 145, 769, 1147
SacI	GAGCTC	1 cut(s)	1287
SacII	CCGCGG	2 cut(s)	102, 258
SapI	GCTCTTC	2 cut(s)	963, 1286
SaqAI	TTAA	5 cut(s)	290, 537, 675, 1392, 1440
SatI	GCNGC	9 cut(s)	75, 102, 255, 807, 940, 974, 1045, 1407, 1463
Sau3AI	GATC	7 cut(s)	196, 279, 439, 520, 801, 1087, 1396
Sau96I	GGNCC	6 cut(s)	91, 136, 157, 664, 773, 1009
SchI	GAGTC	1 cut(s)	863
ScrFI	CCNGG	5 cut(s)	140, 432, 638, 780, 1116
SduI	GDGCHC	5 cut(s)	636, 1134, 1287, 1317, 1460
SfcI	CTRYAG	1 cut(s)	1266
Sfr303I	CCGCGG	2 cut(s)	102, 258
SfuI	TTCGAA	1 cut(s)	1469
SgrBI	CCGCGG	2 cut(s)	102, 258
SinI	GGWCC	5 cut(s)	91, 136, 157, 664, 773
SmlI	CTYRAG	1 cut(s)	674
SmoI	CTYRAG	1 cut(s)	674
SsiI	CCGC	8 cut(s)	94, 99, 101, 255, 257, 806, 1047, 1253
SspI	AATATT	2 cut(s)	465, 1438
SspMI	CTAG	3 cut(s)	170, 795, 855
SstI	GAGCTC	1 cut(s)	1287
StyD4I	CCNGG	5 cut(s)	138, 430, 636, 778, 1114
StyI	CCWWGG	2 cut(s)	810, 1189
TaaI	ACNGT	1 cut(s)	773
TaiI	ACGT	6 cut(s)	264, 301, 408, 516, 647, 1131
TaqII	GACCGA	1 cut(s)	145
TauI	GCSGC	3 cut(s)	104, 257, 809
TfiI	GAWTC	4 cut(s)	152, 416, 962, 1164
Tru1I	TTAA	5 cut(s)	290, 537, 675, 1392, 1440
Tru9I	TTAA	5 cut(s)	290, 537, 675, 1392, 1440
TscAI	CASTG	2 cut(s)	790, 1159
TseFI	GTSAC	4 cut(s)	172, 1049, 1199, 1258
TseI	GCWGC	6 cut(s)	74, 939, 973, 1044, 1406, 1462
Tsp45I	GTSAC	4 cut(s)	172, 1049, 1199, 1258
TspDTI	ATGAA	7 cut(s)	70, 131, 567, 701, 815, 1304, 1427
TspGWI	ACGGA	2 cut(s)	730, 1436
TspRI	CASTG	2 cut(s)	790, 1159
Tth111I	GACNNNGTC	1 cut(s)	134
Vha464I	CTTAAG	1 cut(s)	674
VneI	GTGCAC	1 cut(s)	1130
VpaK11BI	GGWCC	5 cut(s)	91, 136, 157, 664, 773
XapI	RAATTY	2 cut(s)	561, 574
XmiI	GTMKAC	2 cut(s)	264, 896
XmnI	GAANNNNTTC	1 cut(s)	300
XspI	CTAG	3 cut(s)	170, 795, 855
ZraI	GACGTC	1 cut(s)	262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.