RchiOBHm_Chr3g0480681
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0021371
Physical Location & Seq
Reverse (-)
26512996 .. 26513565
570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44569

Sequence Viewer

Length: 570 bp
ATGAGATACAACAATTTATTACTCTACTCTTTATCTCTATCTTCAACCATCTTCTTAAAATCAACAAAAATCCTTCTGAATGAGGACCTCCAGTCTTCCATATCTGGTTTTGGATTGGCACATCTTATCTTGGGGTCCTCAAAGTCTACTTCTTCAGCTTCCAGGAAACAGAACTCGAGCCAATCCAATGCAACATCTTGTTTTACTGTTTCAACTCACTCAACCATTTACTTGGCACCTGAGGCTCGAATTTCTGGCTGCAAGTTTACTCAGAAATGTGATGTGTACTCTTTTGGGATAGTGCTCATGGAGATTTTAACCGGTAAGCTACCAGATGAGGGGCCCGAAAATGGTGGGAAGGGACTAGAGAGTCTGGTGAGGAAGACATTTCGAGAAGAACGCCCCTTATCTGAGATTATAGACCCTGCACTTCTGCAAGAGGTTTATGCAAAGAAGCAAGTTGTTGAAGCCTTTCACATTGCTCTTAATTGCACTGAACTCGACCCAGAACTGCGTCCTAGGATGAAAACAGTTTTAGATAGTCTTGATCGCATCAAATTGCAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

189

Amino Acids

20.93

Weight (kDa)

8.32

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 180 3.6e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 21 - 181 2.1e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021371)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0480681
rosa_laevigata RLG00000023463
rosa_multiflora Rmu_sc0006353.1_g000006
rosa_roxburghii Rroxscaffold_6G00401180
rosa_samantha Rh3DG262100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 146
AcsI RAATTY 1 cut(s) 249
AcuI CTGAAG 1 cut(s) 138
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 338, 350
AgeI ACCGGT 1 cut(s) 320
AgsI TTSAA 3 cut(s) 45, 213, 467
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 3 cut(s) 158, 328, 565
AluI AGCT 3 cut(s) 158, 328, 565
Alw21I GWGCWC 1 cut(s) 306
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 1 cut(s) 341
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 258, 562
ApoI RAATTY 1 cut(s) 249
AsiGI ACCGGT 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 471
AspA2I CCTAGG 1 cut(s) 518
AspS9I GGNCC 4 cut(s) 85, 135, 341, 342
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 2 cut(s) 85, 135
AvrII CCTAGG 1 cut(s) 518
AxyI CCTNAGG 1 cut(s) 240
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 2 cut(s) 87, 389
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 245
BccI CCATC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 1 cut(s) 163
BfaI CTAG 2 cut(s) 365, 519
BisI GCNGC 2 cut(s) 259, 563
BlnI CCTAGG 1 cut(s) 518
BlsI GCNGC 2 cut(s) 260, 564
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 85, 135
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 4 cut(s) 85, 135, 341, 342
BmiI GGNNCC 4 cut(s) 136, 237, 342, 343
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 2 cut(s) 87, 389
BpmI CTGGAG 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 518
BsaWI WCCGGW 1 cut(s) 320
Bsc4I CCNNNNNNNGG 2 cut(s) 338, 350
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 91
Bse21I CCTNAGG 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 2 cut(s) 338, 350
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 231, 284, 402
BseNI ACTGG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 235
BshTI ACCGGT 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 338, 350
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 175
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 306, 345
Bsp143I GATC 1 cut(s) 547
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 3 cut(s) 232, 283, 403
BspLI GGNNCC 4 cut(s) 136, 237, 342, 343
BspT107I GGYRCC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 208, 532
BstDEI CTNAG 3 cut(s) 240, 270, 411
BstF5I GGATG 1 cut(s) 528
BstKTI GATC 1 cut(s) 550
BstMBI GATC 1 cut(s) 547
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 2 cut(s) 87, 389
BstXI CCANNNNNNTGG 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 4 cut(s) 85, 135, 341, 342
CseI GACGC 1 cut(s) 503
Csp6I GTAC 1 cut(s) 286
CspAI ACCGGT 1 cut(s) 320
CviAII CATG 1 cut(s) 307
CviJI RGCY 8 cut(s) 158, 180, 245, 258, 328, 343, 470, 565
CviKI_1 RGCY 8 cut(s) 158, 180, 245, 258, 328, 343, 470, 565
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 3 cut(s) 240, 270, 411
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
DrdI GACNNNNNNGTC 1 cut(s) 369
DriI GACNNNNNGTC 1 cut(s) 91
DseDI GACNNNNNNGTC 1 cut(s) 369
Eam1105I GACNNNNNGTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 85, 135
Eco57I CTGAAG 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 175
EcoO109I RGGNCCY 3 cut(s) 85, 135, 341
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 518
FaeI CATG 1 cut(s) 310
FaiI YATR 4 cut(s) 101, 308, 419, 447
FaqI GGGAC 1 cut(s) 375
FatI CATG 1 cut(s) 306
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 2 cut(s) 259, 563
FokI GGATG 1 cut(s) 535
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 2 cut(s) 259, 563
FspBI CTAG 2 cut(s) 365, 519
GluI GCNGC 2 cut(s) 259, 563
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 321
HgaI GACGC 1 cut(s) 503
Hin1II CATG 1 cut(s) 310
HinfI GANTC 1 cut(s) 370
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 3 cut(s) 147, 267, 286
Hpy188I TCNGA 3 cut(s) 78, 273, 412
Hpy188III TCNNGA 2 cut(s) 392, 545
Hpy8I GTNNAC 3 cut(s) 147, 267, 286
HpyAV CCTTC 2 cut(s) 83, 352
HpyCH4III ACNGT 2 cut(s) 208, 532
HpyCH4V TGCA 7 cut(s) 191, 261, 428, 436, 449, 492, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 3 cut(s) 240, 270, 411
Hsp92II CATG 1 cut(s) 310
Kzo9I GATC 1 cut(s) 547
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 561
MaeI CTAG 2 cut(s) 365, 519
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 6 cut(s) 33, 43, 87, 144, 394, 407
MhlI GDGCHC 2 cut(s) 306, 345
MluCI AATT 4 cut(s) 13, 249, 487, 557
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 7 cut(s) 76, 98, 148, 235, 331, 372, 433
MroXI GAANNNNTTC 1 cut(s) 471
MseI TTAA 4 cut(s) 56, 317, 486, 568
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 1 cut(s) 242
NdeII GATC 1 cut(s) 547
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 4 cut(s) 136, 237, 342, 343
PaeR7I CTCGAG 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 471
PfoI TCCNGGA 1 cut(s) 161
PinAI ACCGGT 1 cut(s) 320
PkrI GCNGC 2 cut(s) 260, 564
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PpuMI RGGWCCY 2 cut(s) 85, 135
Psp5II RGGWCCY 2 cut(s) 85, 135
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 136, 237, 342, 343
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 4 cut(s) 85, 135, 341, 342
PspPPI RGGWCCY 2 cut(s) 85, 135
PspXI VCTCGAGB 1 cut(s) 175
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SaqAI TTAA 4 cut(s) 56, 317, 486, 568
SatI GCNGC 2 cut(s) 259, 563
Sau3AI GATC 1 cut(s) 547
Sau96I GGNCC 4 cut(s) 85, 135, 341, 342
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 306, 345
SetI ASST 6 cut(s) 90, 160, 241, 330, 444, 567
SfaNI GCATC 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 175
SinI GGWCC 2 cut(s) 85, 135
SlaI CTCGAG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
Sse9I AATT 4 cut(s) 13, 249, 487, 557
SspMI CTAG 2 cut(s) 365, 519
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 518
TaaI ACNGT 2 cut(s) 208, 532
TaqI TCGA 4 cut(s) 176, 247, 391, 501
TasI AATT 4 cut(s) 13, 249, 487, 557
TatI WGTACW 1 cut(s) 285
Tru1I TTAA 4 cut(s) 56, 317, 486, 568
Tru9I TTAA 4 cut(s) 56, 317, 486, 568
TscAI CASTG 1 cut(s) 499
TseI GCWGC 2 cut(s) 258, 562
TspDTI ATGAA 1 cut(s) 539
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 85, 135
XapI RAATTY 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 175
XmaJI CCTAGG 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 471
XspI CTAG 2 cut(s) 365, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.