RLG00000023463
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0021371
Physical Location & Seq
Forward (+)
24132814 .. 24133749
936 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023463

Sequence Viewer

Length: 621 bp
ATGTTACCCCAGCCATTTAATCTCTCTGCAAATGGCACATCTATTCATTTGAAGCCCTACACGCACTGTGGTCCTCTTAATCTGCTCAATATTGAAACTATTCAAGACTCCAATATGGTTTCTCTCAGGAATCATAAGGGAACATCCAATTCTTCTAAACCGTTACCATGGGCAGCTCGGTTAAAAATTGCCCAGAGGAGCGCAAGAGGTTTGACGAACATACACGAACACAGCCCTAGAAAGTATGTTCACGGCAACATTAAATCGACAAAAATCCTTCTCAATGAGGAGCTCCAGCCTTACGTATCTGGTTTCGGATTGGCACGTCTTATCTTGGGGTCCTCAAAGTTTACTTCTTCAGCTTCCAGGAAAGAGAGCTCAAGCCAAACCAACGCAACCTCTTGTTTTACTGTTTCAACTCACTCAACCATTTACTTGGCACCTGAGGCTCGAATTTCTGGCAGCAAGTTTACTCAGAAATGTGATGTGTACTCTTTTGGGATGGTGCTCATGGAGATTTTAACCGGTAAGCTACCAGATGAGGGGCCTGAAAATGGTGGGAAGGGACTAGAGAGTGGTGAGGAAGACATTTCGAGAAGAACGTCCCTAATCCGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

207

Amino Acids

22.55

Weight (kDa)

9.43

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 52 - 181 8.9e-13 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 58 - 181 1.1e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021371)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0480681
rosa_laevigata RLG00000023463
rosa_multiflora Rmu_sc0006353.1_g000006
rosa_roxburghii Rroxscaffold_6G00401180
rosa_samantha Rh3DG262100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 542, 554
AgeI ACCGGT 1 cut(s) 524
AgsI TTSAA 4 cut(s) 52, 95, 104, 417
AjiI CACGTC 1 cut(s) 326
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 5 cut(s) 176, 292, 362, 378, 532
AluI AGCT 5 cut(s) 176, 292, 362, 378, 532
Alw21I GWGCWC 3 cut(s) 294, 380, 510
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 2 cut(s) 173, 462
ApoI RAATTY 1 cut(s) 453
AsiGI ACCGGT 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 71, 339, 545
AsuHPI GGTGA 1 cut(s) 590
AvaII GGWCC 2 cut(s) 71, 339
AxyI CCTNAGG 1 cut(s) 444
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 2 cut(s) 294, 380
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 3 cut(s) 294, 380, 510
BbvI GCAGC 2 cut(s) 185, 474
BccI CCATC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 367
BfaI CTAG 2 cut(s) 237, 569
BisI GCNGC 2 cut(s) 174, 463
BlsI GCNGC 2 cut(s) 175, 464
Bme1390I CCNGG 1 cut(s) 367
Bme18I GGWCC 2 cut(s) 71, 339
BmgBI CACGTC 1 cut(s) 326
BmgT120I GGNCC 3 cut(s) 71, 339, 545
BmiI GGNNCC 3 cut(s) 340, 441, 546
BmrFI CCNGG 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 524
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 554
Bse118I RCCGGY 1 cut(s) 524
Bse21I CCTNAGG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 143, 507
BseLI CCNNNNNNNGG 2 cut(s) 542, 554
BseMII CTCAG 3 cut(s) 139, 435, 488
BseRI GAGGAG 2 cut(s) 211, 302
BseXI GCAGC 2 cut(s) 185, 474
BseYI CCCAGC 1 cut(s) 9
BshFI GGCC 1 cut(s) 547
BshNI GGYRCC 1 cut(s) 439
BshTI ACCGGT 1 cut(s) 524
BsiHKAI GWGCWC 3 cut(s) 294, 380, 510
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 2 cut(s) 579, 589
BslI CCNNNNNNNGG 2 cut(s) 542, 554
BsmFI GGGAC 2 cut(s) 579, 589
BsnI GGCC 1 cut(s) 547
Bsp1286I GDGCHC 3 cut(s) 294, 380, 510
Bsp19I CCATGG 1 cut(s) 167
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 3 cut(s) 138, 436, 487
BspLI GGNNCC 3 cut(s) 340, 441, 546
BspT107I GGYRCC 1 cut(s) 439
BsrFI RCCGGY 1 cut(s) 524
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 3 cut(s) 68, 162, 412
BstBAI YACGTR 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 125, 444, 474
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 2 cut(s) 143, 507
BstHHI GCGC 1 cut(s) 203
BstMWI GCNNNNNNNGC 2 cut(s) 61, 446
BstNI CCWGG 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 365
BstSNI TACGTA 1 cut(s) 304
BstV1I GCAGC 2 cut(s) 185, 474
BstV2I GAAGAC 1 cut(s) 591
BstXI CCANNNNNNTGG 1 cut(s) 436
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 1 cut(s) 547
BtgI CCRYGG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 326
BtsCI GGATG 2 cut(s) 143, 507
BtsIMutI CAGTG 1 cut(s) 64
CfoI GCGC 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 71, 339, 545
Csp6I GTAC 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 524
CviAII CATG 2 cut(s) 168, 511
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 3 cut(s) 125, 444, 474
Ecl136II GAGCTC 2 cut(s) 292, 378
Eco105I TACGTA 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 2 cut(s) 294, 380
Eco47I GGWCC 2 cut(s) 71, 339
Eco53kI GAGCTC 2 cut(s) 292, 378
Eco57I CTGAAG 1 cut(s) 342
Eco81I CCTNAGG 1 cut(s) 444
EcoICRI GAGCTC 2 cut(s) 292, 378
EcoO109I RGGNCCY 2 cut(s) 339, 545
EcoRII CCWGG 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 294, 380
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 2 cut(s) 171, 514
FaiI YATR 6 cut(s) 116, 135, 169, 221, 246, 512
FaqI GGGAC 2 cut(s) 579, 589
FatI CATG 2 cut(s) 167, 510
Fnu4HI GCNGC 2 cut(s) 174, 463
FokI GGATG 2 cut(s) 130, 514
FriOI GRGCYC 2 cut(s) 294, 380
Fsp4HI GCNGC 2 cut(s) 174, 463
FspBI CTAG 2 cut(s) 237, 569
GlaI GCGC 1 cut(s) 202
GluI GCNGC 2 cut(s) 174, 463
GsaI CCCAGC 1 cut(s) 13
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 1 cut(s) 547
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 203
Hin1II CATG 2 cut(s) 171, 514
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 2 cut(s) 107, 130
HpaII CCGG 1 cut(s) 525
HphI GGTGA 1 cut(s) 590
Hpy166II GTNNAC 4 cut(s) 250, 351, 471, 490
Hpy188I TCNGA 3 cut(s) 317, 477, 614
Hpy188III TCNNGA 3 cut(s) 104, 127, 594
Hpy8I GTNNAC 4 cut(s) 250, 351, 471, 490
HpyAV CCTTC 2 cut(s) 287, 556
HpyCH4III ACNGT 3 cut(s) 68, 162, 412
HpyCH4IV ACGT 3 cut(s) 303, 325, 602
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 446
HpyF3I CTNAG 3 cut(s) 125, 444, 474
HpySE526I ACGT 3 cut(s) 303, 325, 602
Hsp92II CATG 2 cut(s) 171, 514
HspAI GCGC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 198, 289, 297
Lsp1109I GCAGC 2 cut(s) 185, 474
MaeI CTAG 2 cut(s) 237, 569
MaeII ACGT 3 cut(s) 303, 325, 602
MaeIII GTNAC 2 cut(s) 3, 162
MboII GAAGA 4 cut(s) 144, 348, 596, 609
MhlI GDGCHC 3 cut(s) 294, 380, 510
MluCI AATT 3 cut(s) 148, 186, 453
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 9 cut(s) 84, 189, 200, 280, 352, 409, 439, 535, 574
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 6 cut(s) 18, 78, 182, 261, 521, 619
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 367
MwoI GCNNNNNNNGC 2 cut(s) 61, 446
NcoI CCATGG 1 cut(s) 167
NlaIII CATG 2 cut(s) 171, 514
NlaIV GGNNCC 3 cut(s) 340, 441, 546
PcsI WCGNNNNNNNCGW 1 cut(s) 599
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 130
PfoI TCCNGGA 1 cut(s) 365
PinAI ACCGGT 1 cut(s) 524
PkrI GCNGC 2 cut(s) 175, 464
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 294, 380
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 9
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 340, 441, 546
PspPI GGNCC 3 cut(s) 71, 339, 545
PspPPI RGGWCCY 1 cut(s) 339
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SacI GAGCTC 2 cut(s) 294, 380
SaqAI TTAA 6 cut(s) 18, 78, 182, 261, 521, 619
SatI GCNGC 2 cut(s) 174, 463
Sau96I GGNCC 3 cut(s) 71, 339, 545
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 367
SduI GDGCHC 3 cut(s) 294, 380, 510
SinI GGWCC 2 cut(s) 71, 339
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SnaBI TACGTA 1 cut(s) 304
Sse9I AATT 3 cut(s) 148, 186, 453
SspI AATATT 1 cut(s) 91
SspMI CTAG 2 cut(s) 237, 569
SstI GAGCTC 2 cut(s) 294, 380
StyD4I CCNGG 1 cut(s) 365
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 3 cut(s) 68, 162, 412
TaiI ACGT 3 cut(s) 306, 328, 605
TaqI TCGA 3 cut(s) 266, 451, 593
TasI AATT 3 cut(s) 148, 186, 453
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 6 cut(s) 18, 78, 182, 261, 521, 619
Tru9I TTAA 6 cut(s) 18, 78, 182, 261, 521, 619
TscAI CASTG 1 cut(s) 71
TseI GCWGC 2 cut(s) 173, 462
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 71, 339
XapI RAATTY 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 2 cut(s) 237, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.