RchiOBHm_Chr3g0493791

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0020529
Physical Location & Seq
Forward (+)
41285218 .. 41289751
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45651

Sequence Viewer

Length: 471 bp
ATGAAGATTTTGTTTGTGCAACTTTGGATTGGTGGCCTCCTGAGAAATGTGACTATGGAACTTGTAGCTGGGGCAAGGCTACTCTTCTTAATCTGGGCTAAGATTATCTTTGGATTAAATGCCCTCCCGAGACTAACTTTACAGAGTGATGGCTCTGCAACTGGATCTTGGAACTTTACCAATGCCGAATCTTTCATTCGCTACACTCTATTTGAATGTTGCTCAGGGAATGAGCTATGTGGGCGTGGAATTGGAACCACAGTAGCAGCAAGTCAGTATGTCAATGATACAGCTTCTCTGCATAAGATAGTACAGAATGTATACAAGGGTGTTGGACCAAAGCCACTAATCTTATCACTTGGAGGGTTCTTTGATGCACCTTGGTTCAAAGAACTTGCAGATAAATCCACCAGTTCATTAGATGCGGTCACACACCATATATTTAATCTAGGGCCAGGTAGGTATTGTTGA

Protein Analysis

156

Amino Acids

17.07

Weight (kDa)

8.75

Isoelectric Point (pI)

20.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 33 - 154 5.8e-37 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020529)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0493791
rosa_multiflora Rmu_sc0001896.1_g000035 Rmu_sc0010445.1_g000009
rosa_samantha Rh2BG613100 Rh3AG312800 Rh7CG150200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 172
AfaI GTAC 1 cut(s) 312
AgsI TTSAA 2 cut(s) 215, 388
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 3 cut(s) 68, 235, 293
AluI AGCT 3 cut(s) 68, 235, 293
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 1 cut(s) 172
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 2 cut(s) 34, 452
ApeKI GCWGC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 335, 452
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 335
BbvI GCAGC 1 cut(s) 278
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 1 cut(s) 449
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 456
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 335, 452
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 2 cut(s) 364, 412
Bpu10I CCTNAGC 1 cut(s) 223
BsaJI CCNNGG 1 cut(s) 380
Bse1I ACTGG 2 cut(s) 166, 411
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 380
BseMII CTCAG 2 cut(s) 32, 237
BseNI ACTGG 2 cut(s) 166, 411
BseXI GCAGC 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 68
BshFI GGCC 2 cut(s) 36, 454
BsiHKCI CYCGRG 1 cut(s) 127
BsmAI GTCTC 1 cut(s) 124
BsnI GGCC 2 cut(s) 36, 454
BsoBI CYCGRG 1 cut(s) 127
Bsp143I GATC 1 cut(s) 164
BspACI CCGC 1 cut(s) 425
BspANI GGCC 2 cut(s) 36, 454
BspCNI CTCAG 2 cut(s) 33, 236
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 166, 411
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 1 cut(s) 164
BssNAI GTATAC 1 cut(s) 322
BssT1I CCWWGG 1 cut(s) 380
Bst1107I GTATAC 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 89
BstDEI CTNAG 3 cut(s) 41, 99, 223
BstKTI GATC 1 cut(s) 167
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 1 cut(s) 164
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 454
BstV1I GCAGC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BstZ17I GTATAC 1 cut(s) 322
BsuRI GGCC 2 cut(s) 36, 454
Cfr13I GGNCC 2 cut(s) 335, 452
Csp6I GTAC 1 cut(s) 311
CviJI RGCY 9 cut(s) 36, 68, 79, 98, 153, 235, 293, 343, 454
CviKI_1 RGCY 9 cut(s) 36, 68, 79, 98, 153, 235, 293, 343, 454
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 3 cut(s) 41, 99, 223
DpnI GATC 1 cut(s) 166
DpnII GATC 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 380
FaiI YATR 7 cut(s) 56, 238, 279, 303, 322, 438, 440
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 1 cut(s) 449
GluI GCNGC 1 cut(s) 267
GsaI CCCAGC 1 cut(s) 72
HaeIII GGCC 2 cut(s) 36, 454
HinfI GANTC 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 40, 127
Hpy8I GTNNAC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4V TGCA 5 cut(s) 19, 158, 301, 377, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 3 cut(s) 41, 99, 223
Kzo9I GATC 1 cut(s) 164
LpnPI CCDG 7 cut(s) 53, 54, 79, 147, 210, 424, 441
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 2 cut(s) 364, 412
MaeI CTAG 1 cut(s) 449
MaeIII GTNAC 2 cut(s) 49, 427
MalI GATC 1 cut(s) 166
MboI GATC 1 cut(s) 164
MboII GAAGA 2 cut(s) 16, 76
MflI RGATCY 1 cut(s) 164
MluCI AATT 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 3 cut(s) 47, 134, 356
MseI TTAA 3 cut(s) 89, 116, 444
MspR9I CCNGG 1 cut(s) 456
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 1 cut(s) 241
NdeII GATC 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 49, 427
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 2 cut(s) 335, 452
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SaqAI TTAA 3 cut(s) 89, 116, 444
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 1 cut(s) 164
Sau96I GGNCC 2 cut(s) 335, 452
ScrFI CCNGG 1 cut(s) 456
SetI ASST 6 cut(s) 70, 237, 295, 382, 460, 464
SfaNI GCATC 2 cut(s) 364, 412
SinI GGWCC 1 cut(s) 335
Sse9I AATT 1 cut(s) 249
SsiI CCGC 1 cut(s) 425
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 454
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 262
TasI AATT 1 cut(s) 249
TatI WGTACW 1 cut(s) 310
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 3 cut(s) 89, 116, 444
Tru9I TTAA 3 cut(s) 89, 116, 444
TseFI GTSAC 2 cut(s) 49, 427
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 2 cut(s) 49, 427
TspDTI ATGAA 3 cut(s) 17, 184, 405
VpaK11BI GGWCC 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.