Rh3AG312800

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0020529
Physical Location & Seq
Forward (+)
39576734 .. 39643963
67230 bp
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UTR
Exon/CDS
Intron
Rh3AG312800.1

Sequence Viewer

Length: 605 bp
ATGAAGGTGGATCCTTTTGACCTTTTTGATGCGTACTTTGGGGGAAGGGGTGAATCAGGAGGCATGAGCTTCAATTCGTTAACTCAGGAAATCAGGGCCTTGACATTCGTTAATCTCATTATTTGGAAAAGCTCCTCAGTTCTAAGCCAATGGAATGAGCTATGTGGGCGTGGAATTGGAACCACAGTAGCAGCAAGTCAGTATGTCAATGATACAGCTTCTCTGCATAAGATAGTACAGAATGTATACAAGGGTGTTGGACCAAAGCCACTAATCTTATCACTTGGAGGGTTCTTTGATGCACCTTGGTTCAAAGAACTTGCAGATAAATCCACCAGTTCATTAGATGCGGTCACACACCATATATTTAATCTAGGGCCAGGTGCTAAGCAGCAAAGGGTGAAGTGTTCGGTGAATGTGAAAGATTTTCCAAATATTATCATTCGCTTAGCCCAGGTGAATGGGAGATCAAGATGGGTTATTCCAGCATCTAGTATTGCTTTATCAATTGCCAGGCAATGGTTATGGAATATGATTGGATTCTTGCAGAAGGAGAGAGGTGAACAAGAAAGTGCATGCTTAAAGTTCCTGGAATTGAACAGAAG

Protein Analysis

201

Amino Acids

22.34

Weight (kDa)

9.37

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 36 - 135 1.6e-26 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020529)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0493791
rosa_multiflora Rmu_sc0001896.1_g000035 Rmu_sc0010445.1_g000009
rosa_samantha Rh2BG613100 Rh3AG312800 Rh7CG150200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 519
AccI GTMKAC 1 cut(s) 246
AciI CCGC 1 cut(s) 350
AclWI GGATC 2 cut(s) 5, 18
AfaI GTAC 2 cut(s) 35, 237
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 3 cut(s) 73, 313, 598
AjnI CCWGG 4 cut(s) 379, 453, 512, 588
AluBI AGCT 4 cut(s) 69, 132, 160, 218
AluI AGCT 4 cut(s) 69, 132, 160, 218
AlwI GGATC 2 cut(s) 5, 18
AoxI GGCC 2 cut(s) 96, 377
ApeKI GCWGC 2 cut(s) 191, 391
AspS9I GGNCC 3 cut(s) 96, 260, 377
AsuHPI GGTGA 5 cut(s) 62, 412, 424, 469, 572
AvaII GGWCC 1 cut(s) 260
BamHI GGATCC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 203, 403
BccI CCATC 1 cut(s) 468
BciT130I CCWGG 4 cut(s) 381, 455, 514, 590
BfaI CTAG 2 cut(s) 374, 492
BisI GCNGC 2 cut(s) 192, 392
BlpI GCTNAGC 2 cut(s) 387, 448
BlsI GCNGC 2 cut(s) 193, 393
Bme1390I CCNGG 4 cut(s) 381, 455, 514, 590
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 3 cut(s) 96, 260, 377
BmiI GGNNCC 2 cut(s) 12, 181
BmrFI CCNGG 4 cut(s) 381, 455, 514, 590
BmsI GCATC 4 cut(s) 19, 289, 337, 497
Bpu1102I GCTNAGC 2 cut(s) 387, 448
BsaJI CCNNGG 2 cut(s) 305, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 524
BseBI CCWGG 4 cut(s) 381, 455, 514, 590
BseDI CCNNGG 2 cut(s) 305, 453
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMI GCAATG 1 cut(s) 524
BseMII CTCAG 2 cut(s) 98, 150
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 2 cut(s) 203, 403
BshFI GGCC 2 cut(s) 98, 379
BslI CCNNNNNNNGG 1 cut(s) 519
BsnI GGCC 2 cut(s) 98, 379
Bsp143I GATC 2 cut(s) 10, 467
Bsp1720I GCTNAGC 2 cut(s) 387, 448
BspACI CCGC 1 cut(s) 350
BspANI GGCC 2 cut(s) 98, 379
BspCNI CTCAG 2 cut(s) 97, 149
BspLI GGNNCC 2 cut(s) 12, 181
BspPI GGATC 2 cut(s) 5, 18
BsrDI GCAATG 1 cut(s) 524
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 305, 453
BssMI GATC 2 cut(s) 10, 467
BssNAI GTATAC 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 305
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 4 cut(s) 381, 455, 514, 590
Bst4CI ACNGT 1 cut(s) 187
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 5 cut(s) 84, 136, 143, 387, 448
BstKTI GATC 2 cut(s) 13, 470
BstMBI GATC 2 cut(s) 10, 467
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 4 cut(s) 381, 455, 514, 590
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 4 cut(s) 379, 453, 512, 588
BstV1I GCAGC 2 cut(s) 203, 403
BstX2I RGATCY 1 cut(s) 10
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 1 cut(s) 10
BstZ17I GTATAC 1 cut(s) 247
BsuRI GGCC 2 cut(s) 98, 379
Cac8I GCNNGC 1 cut(s) 577
Cfr13I GGNCC 3 cut(s) 96, 260, 377
Csp6I GTAC 2 cut(s) 34, 236
CviAII CATG 2 cut(s) 64, 576
CviJI RGCY 9 cut(s) 69, 98, 132, 147, 160, 218, 268, 379, 452
CviKI_1 RGCY 9 cut(s) 69, 98, 132, 147, 160, 218, 268, 379, 452
CviQI GTAC 2 cut(s) 34, 236
DdeI CTNAG 5 cut(s) 84, 136, 143, 387, 448
DpnI GATC 2 cut(s) 12, 469
DpnII GATC 2 cut(s) 10, 467
Eco130I CCWWGG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 96
EcoRII CCWGG 4 cut(s) 379, 453, 512, 588
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 2 cut(s) 67, 579
FatI CATG 2 cut(s) 63, 575
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 192, 392
Fsp4HI GCNGC 2 cut(s) 192, 392
FspBI CTAG 2 cut(s) 374, 492
GluI GCNGC 2 cut(s) 192, 392
HaeIII GGCC 2 cut(s) 98, 379
Hin1II CATG 2 cut(s) 67, 579
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 2 cut(s) 53, 540
HpaI GTTAAC 1 cut(s) 81
HphI GGTGA 5 cut(s) 62, 412, 424, 469, 572
Hpy166II GTNNAC 3 cut(s) 81, 247, 563
Hpy188III TCNNGA 3 cut(s) 57, 86, 471
Hpy8I GTNNAC 3 cut(s) 81, 247, 563
HpyAV CCTTC 2 cut(s) 39, 544
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 5 cut(s) 226, 302, 323, 547, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 5 cut(s) 84, 136, 143, 387, 448
Hsp92II CATG 2 cut(s) 67, 579
KspAI GTTAAC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 10, 467
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 2 cut(s) 203, 403
LweI GCATC 4 cut(s) 19, 289, 337, 497
MaeI CTAG 2 cut(s) 374, 492
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 12, 469
MboI GATC 2 cut(s) 10, 467
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 10
MluCI AATT 4 cut(s) 73, 174, 507, 593
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 4 cut(s) 53, 145, 281, 551
MseI TTAA 4 cut(s) 80, 111, 369, 581
MspR9I CCNGG 4 cut(s) 381, 455, 514, 590
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 4 cut(s) 381, 455, 514, 590
MwoI GCNNNNNNNGC 1 cut(s) 166
NdeII GATC 2 cut(s) 10, 467
NlaIII CATG 2 cut(s) 67, 579
NlaIV GGNNCC 2 cut(s) 12, 181
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 1 cut(s) 579
PaeI GCATGC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 53, 540
PflMI CCANNNNNTGG 1 cut(s) 519
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 2 cut(s) 193, 393
Psp6I CCWGG 4 cut(s) 379, 453, 512, 588
PspGI CCWGG 4 cut(s) 379, 453, 512, 588
PspN4I GGNNCC 2 cut(s) 12, 181
PspPI GGNCC 3 cut(s) 96, 260, 377
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 2 cut(s) 35, 237
RsaNI GTAC 2 cut(s) 34, 236
SaqAI TTAA 4 cut(s) 80, 111, 369, 581
SatI GCNGC 2 cut(s) 192, 392
Sau3AI GATC 2 cut(s) 10, 467
Sau96I GGNCC 3 cut(s) 96, 260, 377
ScrFI CCNGG 4 cut(s) 381, 455, 514, 590
SfaNI GCATC 4 cut(s) 19, 289, 337, 497
SinI GGWCC 1 cut(s) 260
SphI GCATGC 1 cut(s) 579
Sse9I AATT 4 cut(s) 73, 174, 507, 593
SsiI CCGC 1 cut(s) 350
SspI AATATT 1 cut(s) 436
SspMI CTAG 2 cut(s) 374, 492
StyD4I CCNGG 4 cut(s) 379, 453, 512, 588
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 187
TasI AATT 4 cut(s) 73, 174, 507, 593
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 2 cut(s) 53, 540
Tru1I TTAA 4 cut(s) 80, 111, 369, 581
Tru9I TTAA 4 cut(s) 80, 111, 369, 581
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 191, 391
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 17, 330
Van91I CCANNNNNTGG 1 cut(s) 519
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 1 cut(s) 579
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 2 cut(s) 374, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.