RchiOBHm_Chr4g0425871

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0021288
Physical Location & Seq
Reverse (-)
51461856 .. 51462741
886 bp
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UTR
Exon/CDS
Intron
PRQ39497

Sequence Viewer

Length: 669 bp
ATGTGGACTATTGGTTACTTTCTGATTGTTCTCCTTTTCTTTTTTTCTTTTCGAATGTTGCATTACTTTGATGCTTTTGCAAAGGAAATTTGCTTTCATCGGAGGATGTTTCACTCGCCCAGCTTTGATAAGCTCTTATGCCTCTTAAAGAATTTACTGATTCCTCGAATGGATATCTCATTGACGACACCTGCGTGCTTGCATGGAGCTGAGGTTTTCGTTTGTAAAGAAAGAACAAAAGGCAAAAGAGAGTATTTATCAAAGATAAATGATGCCTTTATCTGCAAGCAGGTCTGGAAGGTTAAGAATTTTTCAAAGTTGACATGTGAACCATTCGATGCTGGAGACCAGAAACGGCGGATAAAGCTCTATCCCAAGGGATTTGGCAAAGGAAATGGTACTTATCTTTCTATTTTCTTGGCATTGGCTGATTGGAAACCAATTCCTCCTGTGTCCAAAATATTTGTAGCGTTTACCCTGCGCATAGTAGGCCAAATGCATGCCAAGCACAAGTCTTGGACGGGTAAATATATAGACCCTTTTCTTTCTGAACAACTCTACATAATGCCCTATAACAGTAATGACAGTATATTTGCCGCAGTCTTAATTTTCCAGGTAAACACTGTTTCGGTGCCAAGTCGTTGTCGTGGGTTTGGCTCAGTTCATTAG

Protein Analysis

222

Amino Acids

25.72

Weight (kDa)

9.69

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 98 - 172 3.6e-12 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021288)

Species Orthologous Gene IDs
pyrus_communis pycom16g12240
rosa_chinensis RchiOBHm_Chr4g0425871
rosa_roxburghii Rroxscaffold_5G00369180
rosa_samantha Rh4CG282600
rosa_wichuraiana Rw4G022800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 199
Acc16I TGCGCA 1 cut(s) 482
Acc36I ACCTGC 2 cut(s) 199, 280
AccB1I GGYRCC 1 cut(s) 631
AciI CCGC 2 cut(s) 358, 597
AcsI RAATTY 3 cut(s) 87, 151, 307
AfaI GTAC 1 cut(s) 400
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 1 cut(s) 315
AjnI CCWGG 1 cut(s) 612
AleI CACNNNNGTG 1 cut(s) 193
AluBI AGCT 4 cut(s) 123, 133, 209, 367
AluI AGCT 4 cut(s) 123, 133, 209, 367
Alw26I GTCTC 1 cut(s) 339
AoxI GGCC 1 cut(s) 490
ApoI RAATTY 3 cut(s) 87, 151, 307
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AspLEI GCGC 1 cut(s) 483
AsuII TTCGAA 1 cut(s) 52
BanI GGYRCC 1 cut(s) 631
BbvCI CCTCAGC 1 cut(s) 210
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 1 cut(s) 614
BcoDI GTCTC 1 cut(s) 339
BfuAI ACCTGC 2 cut(s) 199, 280
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
Bme1390I CCNGG 1 cut(s) 614
BmiI GGNNCC 1 cut(s) 633
BmrFI CCNGG 1 cut(s) 614
BmsI GCATC 3 cut(s) 61, 262, 328
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 1 cut(s) 210
Bpu14I TTCGAA 1 cut(s) 52
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 119
BshFI GGCC 1 cut(s) 492
BshNI GGYRCC 1 cut(s) 631
BsmAI GTCTC 1 cut(s) 339
BsnI GGCC 1 cut(s) 492
Bso31I GGTCTC 1 cut(s) 339
Bsp119I TTCGAA 1 cut(s) 52
BspACI CCGC 2 cut(s) 358, 597
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 633
BspMI ACCTGC 2 cut(s) 199, 280
BspT104I TTCGAA 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 631
BspTNI GGTCTC 1 cut(s) 339
BssECI CCNNGG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 3 cut(s) 578, 587, 625
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 4 cut(s) 196, 200, 287, 501
BstDEI CTNAG 2 cut(s) 210, 658
BstF5I GGATG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 483
BstMAI GTCTC 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 364, 489, 505
BstNI CCWGG 1 cut(s) 614
BstNSI RCATGY 2 cut(s) 327, 503
BstSCI CCNGG 1 cut(s) 612
BsuRI GGCC 1 cut(s) 492
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 621
BveI ACCTGC 2 cut(s) 199, 280
Cac8I GCNNGC 4 cut(s) 196, 200, 287, 501
CfoI GCGC 1 cut(s) 483
Csp6I GTAC 1 cut(s) 399
CviAII CATG 3 cut(s) 203, 324, 500
CviJI RGCY 7 cut(s) 123, 133, 209, 367, 428, 492, 657
CviKI_1 RGCY 7 cut(s) 123, 133, 209, 367, 428, 492, 657
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 2 cut(s) 210, 658
EciI GGCGGA 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 612
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 206, 327, 503
FatI CATG 3 cut(s) 202, 323, 499
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 597
FspI TGCGCA 1 cut(s) 482
GlaI GCGC 1 cut(s) 482
GluI GCNGC 1 cut(s) 597
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 1 cut(s) 492
HhaI GCGC 1 cut(s) 483
Hin1II CATG 3 cut(s) 206, 327, 503
Hin6I GCGC 1 cut(s) 481
HinP1I GCGC 1 cut(s) 481
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HinfI GANTC 1 cut(s) 160
Hpy166II GTNNAC 5 cut(s) 6, 321, 329, 474, 619
Hpy188I TCNGA 3 cut(s) 24, 102, 550
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 5 cut(s) 6, 321, 329, 474, 619
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 3 cut(s) 578, 587, 625
HpyCH4V TGCA 5 cut(s) 61, 80, 202, 285, 499
HpyF10VI GCNNNNNNNGC 3 cut(s) 364, 489, 505
HpyF3I CTNAG 2 cut(s) 210, 658
Hsp92II CATG 3 cut(s) 206, 327, 503
HspAI GCGC 1 cut(s) 481
LmnI GCTCC 1 cut(s) 206
LweI GCATC 3 cut(s) 61, 262, 328
MaeIII GTNAC 1 cut(s) 14
MluCI AATT 5 cut(s) 87, 151, 307, 441, 606
MnlI CCTC 5 cut(s) 96, 152, 174, 205, 456
Mph1103I ATGCAT 1 cut(s) 501
MseI TTAA 3 cut(s) 146, 303, 605
MslI CAYNNNNRTG 1 cut(s) 193
MspR9I CCNGG 1 cut(s) 614
MvaI CCWGG 1 cut(s) 614
MwoI GCNNNNNNNGC 3 cut(s) 364, 489, 505
NlaIII CATG 3 cut(s) 206, 327, 503
NlaIV GGNNCC 1 cut(s) 633
NsbI TGCGCA 1 cut(s) 482
NsiI ATGCAT 1 cut(s) 501
NspI RCATGY 2 cut(s) 327, 503
NspV TTCGAA 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 193
PaeI GCATGC 1 cut(s) 503
PaqCI CACCTGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 1 cut(s) 598
PscI ACATGT 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 612
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 1 cut(s) 633
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 193
SaqAI TTAA 3 cut(s) 146, 303, 605
SatI GCNGC 1 cut(s) 597
ScrFI CCNGG 1 cut(s) 614
SetI ASST 9 cut(s) 125, 135, 193, 211, 216, 294, 303, 369, 618
SfaNI GCATC 3 cut(s) 61, 262, 328
SfuI TTCGAA 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 193
SphI GCATGC 1 cut(s) 503
Sse9I AATT 5 cut(s) 87, 151, 307, 441, 606
SsiI CCGC 2 cut(s) 358, 597
SspI AATATT 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 612
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 3 cut(s) 578, 587, 625
TaqI TCGA 3 cut(s) 52, 166, 336
TasI AATT 5 cut(s) 87, 151, 307, 441, 606
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 3 cut(s) 146, 303, 605
Tru9I TTAA 3 cut(s) 146, 303, 605
TscAI CASTG 1 cut(s) 628
TspDTI ATGAA 2 cut(s) 86, 653
TspRI CASTG 1 cut(s) 628
XapI RAATTY 3 cut(s) 87, 151, 307
XceI RCATGY 2 cut(s) 327, 503
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.