Rroxscaffold_5G00369180

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0021288
Physical Location & Seq
Reverse (-)
51038609 .. 51039796
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369180.1

Sequence Viewer

Length: 408 bp
ATGCTTCGCTGGCCCAGCTTTGATAAGCTTATGCCCGTTAAAGAATTTACTGAAGCCTCGATTAGATATCTCATTGAGGACACCAGCTGCGTGCTTGGAGCTGAGGTTTTCGTCTGTAAAGAAAGAAGAAAACGCAAAAGAGAGCATTTATCGAAGATACATGATATGCCTTTATGTGCAACCATGCAGGTTTGGAAGGTGGAGAATTTATCAGAGTTGACTGATGAATATTGTATGTCAGAACCATTCAATGATGGAGACCAGAAATGGCGGATACAGATCTATCCCATCAGGGATATGACAAGAGGAAATATAAAAAACCGTCGGTATCGGTTGAGTCAGAACTCAGAAGATCAAACCGATGCAGTGCAGCCCAGTAGCACCGAGAGGGGAGACTATAGGGAATGA

Protein Analysis

135

Amino Acids

16.14

Weight (kDa)

6.75

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MATH_2 PF22486 64 - 98 8.9e-06 MATH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021288)

Species Orthologous Gene IDs
pyrus_communis pycom16g12240
rosa_chinensis RchiOBHm_Chr4g0425871
rosa_roxburghii Rroxscaffold_5G00369180
rosa_samantha Rh4CG282600
rosa_wichuraiana Rw4G022800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
AciI CCGC 1 cut(s) 271
AcsI RAATTY 2 cut(s) 44, 205
AcuI CTGAAG 1 cut(s) 72
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 4 cut(s) 18, 28, 87, 101
AluI AGCT 4 cut(s) 18, 28, 87, 101
Alw26I GTCTC 2 cut(s) 252, 387
AoxI GGCC 1 cut(s) 11
ApeKI GCWGC 2 cut(s) 87, 370
ApoI RAATTY 2 cut(s) 44, 205
AspS9I GGNCC 1 cut(s) 12
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 74, 382
BccI CCATC 2 cut(s) 248, 296
BciVI GTATCC 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 252, 387
BfmI CTRYAG 1 cut(s) 397
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 267
BglII AGATCT 1 cut(s) 279
BisI GCNGC 2 cut(s) 88, 371
BlsI GCNGC 2 cut(s) 89, 372
BmgT120I GGNCC 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 352
BmuI ACTGGG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 278
BsaI GGTCTC 1 cut(s) 252
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse1I ACTGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 278
BseJI GATNNNNATC 1 cut(s) 278
BseMII CTCAG 2 cut(s) 93, 360
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 2 cut(s) 74, 382
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 1 cut(s) 13
BsmAI GTCTC 2 cut(s) 252, 387
BsnI GGCC 1 cut(s) 13
Bso31I GGTCTC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 279, 352
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 13
BspCNI CTCAG 2 cut(s) 94, 359
BspMI ACCTGC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 375
BssMI GATC 2 cut(s) 279, 352
Bst4CI ACNGT 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 11, 92
BstDEI CTNAG 2 cut(s) 102, 346
BstKTI GATC 2 cut(s) 282, 355
BstMAI GTCTC 2 cut(s) 252, 387
BstMBI GATC 2 cut(s) 279, 352
BstMWI GCNNNNNNNGC 2 cut(s) 10, 15
BstSFI CTRYAG 1 cut(s) 397
BstV1I GCAGC 2 cut(s) 74, 382
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 1 cut(s) 13
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 11, 92
Cfr13I GGNCC 1 cut(s) 12
CviAII CATG 2 cut(s) 161, 184
CviJI RGCY 7 cut(s) 13, 18, 28, 56, 87, 101, 373
CviKI_1 RGCY 7 cut(s) 13, 18, 28, 56, 87, 101, 373
DdeI CTNAG 2 cut(s) 102, 346
DpnI GATC 2 cut(s) 281, 354
DpnII GATC 2 cut(s) 279, 352
EciI GGCGGA 1 cut(s) 286
Eco31I GGTCTC 1 cut(s) 252
Eco32I GATATC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 72
EcoRV GATATC 1 cut(s) 68
FaeI CATG 2 cut(s) 164, 187
FaiI YATR 9 cut(s) 32, 162, 167, 175, 185, 236, 299, 314, 399
FatI CATG 2 cut(s) 160, 183
Fnu4HI GCNGC 2 cut(s) 88, 371
Fsp4HI GCNGC 2 cut(s) 88, 371
GluI GCNGC 2 cut(s) 88, 371
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 13
Hin1II CATG 2 cut(s) 164, 187
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 4 cut(s) 214, 241, 342, 349
Hpy8I GTNNAC 1 cut(s) 219
Hpy99I CGWCG 1 cut(s) 327
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4V TGCA 4 cut(s) 179, 187, 365, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 15
HpyF3I CTNAG 2 cut(s) 102, 346
Hsp92II CATG 2 cut(s) 164, 187
Kzo9I GATC 2 cut(s) 279, 352
LmnI GCTCC 1 cut(s) 98
LpnPI CCDG 6 cut(s) 28, 97, 173, 275, 277, 388
Lsp1109I GCAGC 2 cut(s) 74, 382
LweI GCATC 1 cut(s) 352
MalI GATC 2 cut(s) 281, 354
MboI GATC 2 cut(s) 279, 352
MboII GAAGA 3 cut(s) 138, 166, 362
MflI RGATCY 1 cut(s) 279
MluCI AATT 2 cut(s) 44, 205
MlyI GAGTC 1 cut(s) 346
MnlI CCTC 5 cut(s) 67, 70, 97, 299, 381
MseI TTAA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 10, 15
NdeII GATC 2 cut(s) 279, 352
NlaIII CATG 2 cut(s) 164, 187
PkrI GCNGC 2 cut(s) 89, 372
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PspFI CCCAGC 1 cut(s) 14
PspPI GGNCC 1 cut(s) 12
PsuI RGATCY 1 cut(s) 279
PvuII CAGCTG 1 cut(s) 87
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 2 cut(s) 88, 371
Sau3AI GATC 2 cut(s) 279, 352
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 346
SetI ASST 7 cut(s) 20, 30, 89, 103, 108, 192, 201
SfaNI GCATC 1 cut(s) 352
SfcI CTRYAG 1 cut(s) 397
Sse9I AATT 2 cut(s) 44, 205
SsiI CCGC 1 cut(s) 271
SspI AATATT 1 cut(s) 230
TaaI ACNGT 1 cut(s) 323
TaqI TCGA 2 cut(s) 59, 152
TasI AATT 2 cut(s) 44, 205
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 1 cut(s) 372
TseI GCWGC 2 cut(s) 87, 370
TspDTI ATGAA 1 cut(s) 240
TspRI CASTG 1 cut(s) 372
XapI RAATTY 2 cut(s) 44, 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.