RchiOBHm_Chr4g0438721

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016835
Physical Location & Seq
Reverse (-)
61336803 .. 61338535
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40684

Sequence Viewer

Length: 1596 bp
ATGTCATATAGTACTAGATGCCTCCAACTGTTGGAGAAGTGCAAGAACCTAAAGCACCTCCACCAAGCTCATGCCCAAGTATACACTTGTGGCCTTGCCCACAACACCTTTGCCTTAAGCAGACTCTTAGCCTTCTGCTCAGACCCACATCATGGAAGCCTCTCCTATGCCTCAAAACTCTTCCAACACATTCCCCAACCCACCCTTTGCATCTACAACACAATGTTAAAAGCTCTCCTGCTCAGAAATGAACTCACCCAAACCCTAAATGTGTTCACCCAGATGTTACAAAATGGGACCTACCCTGACAATTATACCCTCCCTTATGTTCTAAAGGCCTCTGCTAGGCTTCAAGGCTATACTCTTGGAGAGTTGGTTCATGGGTTTAGCTTGAAATTGGGTTTTGAGTCTGATATCTATGTGGGCAACTCTCTGATTGTTATGTACTGTGCATTTGATAATATGAAAGGTGCACGCTGCGTGTTCGACGAAATGCCTAGGCTAAGTGCTGTGTCATGGACGGTCATGATTTCTGGGTATGCAAAGGTGGGTGATGTGGACACTGCTAGAGTGTTCTTTGATGAGGCTCCTGTGAGAGATAGAGGAATATGGGGTGCCATGATTTCTGGGTATGTGCAAAACAATTGCTTTAAAGAAGGTCTGTATTTGTTCCGGTTGATGCAGGTTGCTGATGTAGAGCCTGATGAGGCTGTTTTTGTCAGTGTTCTATGTGCTTGTGCTCATTTGGGAGATTTGGATATTGGGATTTGGATTCACTCGTATTTGAATCGGCTCGGGTTGCCATCGAGTGTTAGACTCGGCACTGGTCTTATTGACATGTATGCGAAATGTGGGAAATTGGACTTAGCTAGAGGAGTGTTTTATGAGATTCCGCAGAGGGATACGGTTTGCTGGAATGCTATGATATCAGGAATGGCAATGCATGGGGATGGAGAAGGTGCACTCAAGCTGTTTAGAGAGATGGAGGCAGCTCAGGTCAGGCCGGATGATATCACCTTCATTGCTGTGTTTACTGCTTGTAGTTATGCAGGAATGGCATATGAAGGCATGAGGGTGTTGGATAAAATGTGTAATGTATACAATATTAGGCCGAAAAGTGAACATTATGGCTGCATTGTTGACCTTCTAAGTCGTGCTGGTCTCTTTGAAGAAGCAAGGGAAATAATCGAAAGAATACCCAGTTCAAGTAACCCTTCTGAAGAAACAGTAGCTTGGAGAGCTTTTCTCAGCGCTTGTTGCAATCATGGACAGTCTCAACTAGCTGAGATTGCTGCAGAGAAACTCTTTCAGCTAGAACGTCACAGTGGCGTGTATGTCTTGCTCTCAAATTTATATGCAGCTGCCGGAAAGCATGGTGATGCCAGGAGAATGAGAAGTTTGATGAGAAATATAGGAGTAGATAAGGCCCCGGGTTGCAGTTCGGTCGAGATTGATAGAGCTGTTTATGAATTCATTGCAGGGGAGAAAACACACCCTCAAATGGAAGAAATTCAGTTAGTTTTGCAAACTATAATGAAGCAAATGGATTTTTCAGGCAGTGATTATCCATATTTGTTTCTGGTTAACAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

531

Amino Acids

59.46

Weight (kDa)

6.35

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 67 - 113 1.5e-06 PPR repeat family
PPR_2 PF13041 300 - 347 9.2e-12 PPR repeat family
PPR PF01535 303 - 330 3e-07 PPR repeat
E_motif PF20431 424 - 483 1.7e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016835)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30430
malus_domestica MD16G1063600.v1.1
prunus_persica Prupe.1G282900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0438721
rosa_laevigata RLG00000006334
rosa_multiflora Rmu_sc0000144.1_g000047 Rmu_sc0002505.1_g000006
rosa_roxburghii Rroxscaffold_5G00379760
rosa_rugosa Rorug04G0312800
rosa_samantha Rh4AG363400 Rh4BG374800 Rh4CG389000
rosa_wichuraiana Rw0G007190 Rw0G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 2 cut(s) 31, 152
AccI GTMKAC 2 cut(s) 81, 1098
AciI CCGC 1 cut(s) 893
AcsI RAATTY 3 cut(s) 1348, 1469, 1509
AcuI CTGAAG 1 cut(s) 1239
AfaI GTAC 2 cut(s) 13, 446
AfeI AGCGCT 1 cut(s) 1252
AfiI CCNNNNNNNGG 4 cut(s) 31, 152, 345, 1501
AflII CTTAAG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 837
AgsI TTSAA 5 cut(s) 353, 394, 787, 1169, 1206
AjnI CCWGG 1 cut(s) 1382
AloI GAACNNNNNNTCC 2 cut(s) 360, 392
Alw21I GWGCWC 3 cut(s) 475, 742, 964
Alw26I GTCTC 2 cut(s) 1166, 1278
Alw44I GTGCAC 2 cut(s) 471, 960
Ama87I CYCGRG 2 cut(s) 794, 1429
Aor51HI AGCGCT 1 cut(s) 1252
AoxI GGCC 5 cut(s) 91, 336, 1001, 1109, 1425
ApaLI GTGCAC 2 cut(s) 471, 960
ApeKI GCWGC 6 cut(s) 477, 989, 1131, 1292, 1358, 1361
ApoI RAATTY 3 cut(s) 1348, 1469, 1509
Asp700I GAANNNNTTC 1 cut(s) 1509
AspA2I CCTAGG 1 cut(s) 497
AspLEI GCGC 1 cut(s) 1253
AspS9I GGNCC 2 cut(s) 297, 1426
AsuC2I CCSGG 2 cut(s) 1430, 1431
AsuHPI GGTGA 5 cut(s) 247, 268, 563, 1006, 1388
AvaI CYCGRG 2 cut(s) 794, 1429
AvaII GGWCC 1 cut(s) 297
AvrII CCTAGG 1 cut(s) 497
BaeGI GKGCMC 2 cut(s) 475, 964
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 3 cut(s) 475, 742, 964
BbvI GCAGC 6 cut(s) 464, 1001, 1118, 1279, 1348, 1370
BccI CCATC 3 cut(s) 811, 944, 976
BcgI CGANNNNNNTGC 2 cut(s) 1426, 1460
BciT130I CCWGG 1 cut(s) 1384
BciVI GTATCC 1 cut(s) 895
BcnI CCSGG 2 cut(s) 1430, 1431
BcoDI GTCTC 2 cut(s) 1166, 1278
BfaI CTAG 7 cut(s) 15, 345, 498, 567, 870, 1280, 1313
BfmI CTRYAG 1 cut(s) 1293
BfoI RGCGCY 1 cut(s) 1254
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 673
BfuI GTATCC 1 cut(s) 895
BisI GCNGC 6 cut(s) 478, 990, 1132, 1293, 1359, 1362
BlnI CCTAGG 1 cut(s) 497
BlsI GCNGC 6 cut(s) 479, 991, 1133, 1294, 1360, 1363
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 3 cut(s) 1384, 1430, 1431
Bme18I GGWCC 1 cut(s) 297
BmeT110I CYCGRG 2 cut(s) 794, 1429
BmgT120I GGNCC 2 cut(s) 297, 1426
BmiI GGNNCC 4 cut(s) 298, 588, 616, 1428
BmrFI CCNGG 3 cut(s) 1384, 1430, 1431
BmrI ACTGGG 1 cut(s) 1194
BmsI GCATC 4 cut(s) 8, 219, 669, 1369
BmuI ACTGGG 1 cut(s) 1194
BplI GAGNNNNNCTC 2 cut(s) 1230, 1262
Bpu10I CCTNAGC 1 cut(s) 993
BpuEI CTTGAG 1 cut(s) 950
BpuMI CCSGG 2 cut(s) 1430, 1431
BsaI GGTCTC 1 cut(s) 1166
BsaJI CCNNGG 3 cut(s) 497, 1428, 1429
BsaWI WCCGGW 1 cut(s) 672
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 152, 345, 1501
Bse1I ACTGG 2 cut(s) 829, 1200
Bse3DI GCAATG 3 cut(s) 945, 1020, 1473
BseBI CCWGG 1 cut(s) 1384
BseDI CCNNGG 3 cut(s) 497, 1428, 1429
BseGI GGATG 2 cut(s) 955, 1012
BseLI CCNNNNNNNGG 4 cut(s) 31, 152, 345, 1501
BseMI GCAATG 3 cut(s) 945, 1020, 1473
BseMII CTCAG 5 cut(s) 153, 256, 1007, 1261, 1275
BseNI ACTGG 2 cut(s) 829, 1200
BseRI GAGGAG 1 cut(s) 888
BseSI GKGCMC 2 cut(s) 475, 964
BseXI GCAGC 6 cut(s) 464, 1001, 1118, 1279, 1348, 1370
Bsh1285I CGRYCG 1 cut(s) 1446
BshFI GGCC 5 cut(s) 93, 338, 1003, 1111, 1427
BshNI GGYRCC 1 cut(s) 614
BsiEI CGRYCG 1 cut(s) 1446
BsiHKAI GWGCWC 3 cut(s) 475, 742, 964
BsiHKCI CYCGRG 2 cut(s) 794, 1429
BsiSI CCGG 4 cut(s) 673, 1004, 1365, 1430
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 4 cut(s) 31, 152, 345, 1501
BsmAI GTCTC 2 cut(s) 1166, 1278
BsmFI GGGAC 1 cut(s) 310
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 5 cut(s) 93, 338, 1003, 1111, 1427
Bso31I GGTCTC 1 cut(s) 1166
BsoBI CYCGRG 2 cut(s) 794, 1429
Bsp1286I GDGCHC 3 cut(s) 475, 742, 964
BspACI CCGC 1 cut(s) 893
BspANI GGCC 5 cut(s) 93, 338, 1003, 1111, 1427
BspCNI CTCAG 5 cut(s) 152, 255, 1006, 1260, 1276
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 4 cut(s) 298, 588, 616, 1428
BspMAI CTGCAG 1 cut(s) 1297
BspMI ACCTGC 1 cut(s) 673
BspT107I GGYRCC 1 cut(s) 614
BspTI CTTAAG 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 1166
BsrDI GCAATG 3 cut(s) 945, 1020, 1473
BsrI ACTGG 2 cut(s) 829, 1200
BssECI CCNNGG 3 cut(s) 497, 1428, 1429
BssNAI GTATAC 2 cut(s) 82, 1099
BssT1I CCWWGG 1 cut(s) 497
Bst1107I GTATAC 2 cut(s) 82, 1099
Bst2UI CCWGG 1 cut(s) 1384
Bst4CI ACNGT 7 cut(s) 30, 449, 523, 907, 1228, 1272, 1325
Bst6I CTCTTC 1 cut(s) 185
BstAFI CTTAAG 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 9 cut(s) 127, 139, 242, 503, 865, 993, 1148, 1247, 1284
BstENI CCTNNNNNAGG 1 cut(s) 343
BstF5I GGATG 2 cut(s) 955, 1012
BstH2I RGCGCY 1 cut(s) 1254
BstHHI GCGC 1 cut(s) 1253
BstMAI GTCTC 2 cut(s) 1166, 1278
BstMCI CGRYCG 1 cut(s) 1446
BstMWI GCNNNNNNNGC 5 cut(s) 799, 1055, 1238, 1257, 1289
BstNI CCWGG 1 cut(s) 1384
BstNSI RCATGY 1 cut(s) 841
BstSCI CCNGG 3 cut(s) 1382, 1428, 1429
BstSFI CTRYAG 1 cut(s) 1293
BstSLI GKGCMC 2 cut(s) 475, 964
BstV1I GCAGC 6 cut(s) 464, 1001, 1118, 1279, 1348, 1370
BstZ17I GTATAC 2 cut(s) 82, 1099
BsuI GTATCC 1 cut(s) 895
BsuRI GGCC 5 cut(s) 93, 338, 1003, 1111, 1427
BtsCI GGATG 2 cut(s) 955, 1012
BtsI GCAGTG 2 cut(s) 561, 1564
BtsIMutI CAGTG 5 cut(s) 561, 727, 822, 1330, 1564
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 1 cut(s) 475
CciI TCATGA 1 cut(s) 525
CfoI GCGC 1 cut(s) 1253
Cfr13I GGNCC 2 cut(s) 297, 1426
Cfr9I CCCGGG 1 cut(s) 1429
Csp6I GTAC 2 cut(s) 12, 445
CviQI GTAC 2 cut(s) 12, 445
DdeI CTNAG 9 cut(s) 127, 139, 242, 503, 865, 993, 1148, 1247, 1284
DraI TTTAAA 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 497
Eco147I AGGCCT 1 cut(s) 338
Eco31I GGTCTC 1 cut(s) 1166
Eco32I GATATC 3 cut(s) 415, 927, 1012
Eco47I GGWCC 1 cut(s) 297
Eco47III AGCGCT 1 cut(s) 1252
Eco57I CTGAAG 1 cut(s) 1239
Eco88I CYCGRG 2 cut(s) 794, 1429
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 2 cut(s) 297, 1426
EcoRI GAATTC 1 cut(s) 1469
EcoRII CCWGG 1 cut(s) 1382
EcoRV GATATC 3 cut(s) 415, 927, 1012
EcoT14I CCWWGG 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 945
ErhI CCWWGG 1 cut(s) 497
FalI AAGNNNNNCTT 2 cut(s) 1198, 1230
FaqI GGGAC 1 cut(s) 310
FauNDI CATATG 1 cut(s) 1060
FblI GTMKAC 2 cut(s) 81, 1098
Fnu4HI GCNGC 6 cut(s) 478, 990, 1132, 1293, 1359, 1362
FokI GGATG 2 cut(s) 962, 1019
Fsp4HI GCNGC 6 cut(s) 478, 990, 1132, 1293, 1359, 1362
FspBI CTAG 7 cut(s) 15, 345, 498, 567, 870, 1280, 1313
GlaI GCGC 1 cut(s) 1252
GluI GCNGC 6 cut(s) 478, 990, 1132, 1293, 1359, 1362
HaeII RGCGCY 1 cut(s) 1254
HaeIII GGCC 5 cut(s) 93, 338, 1003, 1111, 1427
HapII CCGG 4 cut(s) 673, 1004, 1365, 1430
HhaI GCGC 1 cut(s) 1253
Hin6I GCGC 1 cut(s) 1251
HinP1I GCGC 1 cut(s) 1251
HincII GTYRAC 2 cut(s) 1141, 1585
HindII GTYRAC 2 cut(s) 1141, 1585
HinfI GANTC 6 cut(s) 123, 407, 772, 787, 816, 889
HpaI GTTAAC 1 cut(s) 1585
HpaII CCGG 4 cut(s) 673, 1004, 1365, 1430
HphI GGTGA 5 cut(s) 247, 268, 563, 1006, 1388
Hpy188I TCNGA 5 cut(s) 142, 245, 412, 435, 1219
Hpy188III TCNNGA 3 cut(s) 526, 930, 1447
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 7 cut(s) 142, 650, 950, 1027, 1058, 1154, 1224
HpyCH4III ACNGT 7 cut(s) 30, 449, 523, 907, 1228, 1272, 1325
HpyCH4IV ACGT 1 cut(s) 1318
HpyF10VI GCNNNNNNNGC 5 cut(s) 799, 1055, 1238, 1257, 1289
HpyF3I CTNAG 9 cut(s) 127, 139, 242, 503, 865, 993, 1148, 1247, 1284
HpySE526I ACGT 1 cut(s) 1318
HspAI GCGC 1 cut(s) 1251
KspAI GTTAAC 1 cut(s) 1585
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 6 cut(s) 464, 1001, 1118, 1279, 1348, 1370
LweI GCATC 4 cut(s) 8, 219, 669, 1369
MaeI CTAG 7 cut(s) 15, 345, 498, 567, 870, 1280, 1313
MaeII ACGT 1 cut(s) 1318
MaeIII GTNAC 3 cut(s) 285, 1208, 1319
MboII GAAGA 4 cut(s) 172, 1181, 1232, 1517
MfeI CAATTG 1 cut(s) 643
MhlI GDGCHC 3 cut(s) 475, 742, 964
MluCI AATT 7 cut(s) 310, 395, 643, 857, 1348, 1469, 1509
MlyI GAGTC 3 cut(s) 117, 416, 810
MmeI TCCRAC 4 cut(s) 12, 49, 208, 1059
Mph1103I ATGCAT 1 cut(s) 945
MroXI GAANNNNTTC 1 cut(s) 1509
MseI TTAA 4 cut(s) 116, 227, 651, 1584
MslI CAYNNNNRTG 5 cut(s) 281, 948, 1025, 1073, 1377
MspA1I CMGCKG 1 cut(s) 1361
MspCI CTTAAG 1 cut(s) 115
MspI CCGG 4 cut(s) 673, 1004, 1365, 1430
MspR9I CCNGG 3 cut(s) 1384, 1430, 1431
MunI CAATTG 1 cut(s) 643
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 1 cut(s) 1384
MwoI GCNNNNNNNGC 5 cut(s) 799, 1055, 1238, 1257, 1289
NciI CCSGG 2 cut(s) 1430, 1431
NdeI CATATG 1 cut(s) 1060
NlaIV GGNNCC 4 cut(s) 298, 588, 616, 1428
NmeAIII GCCGAG 1 cut(s) 798
NmuCI GTSAC 1 cut(s) 1319
NsiI ATGCAT 1 cut(s) 945
NspI RCATGY 1 cut(s) 841
PagI TCATGA 1 cut(s) 525
PceI AGGCCT 1 cut(s) 338
PciI ACATGT 1 cut(s) 837
PctI GAATGC 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 1509
PfeI GAWTC 3 cut(s) 772, 787, 889
PflMI CCANNNNNTGG 2 cut(s) 31, 152
PkrI GCNGC 6 cut(s) 479, 991, 1133, 1294, 1360, 1363
PleI GAGTC 3 cut(s) 117, 415, 810
PpsI GAGTC 3 cut(s) 117, 415, 810
PpuMI RGGWCCY 1 cut(s) 297
PscI ACATGT 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 1382
PspGI CCWGG 1 cut(s) 1382
PspN4I GGNNCC 4 cut(s) 298, 588, 616, 1428
PspPI GGNCC 2 cut(s) 297, 1426
PspPPI RGGWCCY 1 cut(s) 297
PstI CTGCAG 1 cut(s) 1297
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 2 cut(s) 13, 446
RsaNI GTAC 2 cut(s) 12, 445
RseI CAYNNNNRTG 5 cut(s) 281, 948, 1025, 1073, 1377
SaqAI TTAA 4 cut(s) 116, 227, 651, 1584
SatI GCNGC 6 cut(s) 478, 990, 1132, 1293, 1359, 1362
Sau96I GGNCC 2 cut(s) 297, 1426
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 3 cut(s) 117, 416, 810
ScrFI CCNGG 3 cut(s) 1384, 1430, 1431
SduI GDGCHC 3 cut(s) 475, 742, 964
SfaNI GCATC 4 cut(s) 8, 219, 669, 1369
SfcI CTRYAG 1 cut(s) 1293
SinI GGWCC 1 cut(s) 297
SmaI CCCGGG 1 cut(s) 1431
SmiMI CAYNNNNRTG 5 cut(s) 281, 948, 1025, 1073, 1377
SmlI CTYRAG 2 cut(s) 115, 965
SmoI CTYRAG 2 cut(s) 115, 965
Sse9I AATT 7 cut(s) 310, 395, 643, 857, 1348, 1469, 1509
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 1 cut(s) 893
SspI AATATT 1 cut(s) 1105
SspMI CTAG 7 cut(s) 15, 345, 498, 567, 870, 1280, 1313
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 3 cut(s) 1382, 1428, 1429
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 7 cut(s) 30, 449, 523, 907, 1228, 1272, 1325
TaiI ACGT 1 cut(s) 1321
TaqI TCGA 4 cut(s) 486, 806, 1188, 1446
TaqII GACCGA 1 cut(s) 1432
TasI AATT 7 cut(s) 310, 395, 643, 857, 1348, 1469, 1509
TatI WGTACW 2 cut(s) 11, 444
TfiI GAWTC 3 cut(s) 772, 787, 889
Tru1I TTAA 4 cut(s) 116, 227, 651, 1584
Tru9I TTAA 4 cut(s) 116, 227, 651, 1584
TscAI CASTG 5 cut(s) 568, 727, 829, 1330, 1564
TseFI GTSAC 1 cut(s) 1319
TseI GCWGC 6 cut(s) 477, 989, 1131, 1292, 1358, 1361
Tsp45I GTSAC 1 cut(s) 1319
TspDTI ATGAA 8 cut(s) 264, 368, 479, 1009, 1077, 1462, 1482, 1550
TspMI CCCGGG 1 cut(s) 1429
TspRI CASTG 5 cut(s) 568, 727, 829, 1330, 1564
Van91I CCANNNNNTGG 2 cut(s) 31, 152
Vha464I CTTAAG 1 cut(s) 115
VneI GTGCAC 2 cut(s) 471, 960
VpaK11BI GGWCC 1 cut(s) 297
XagI CCTNNNNNAGG 1 cut(s) 343
XapI RAATTY 3 cut(s) 1348, 1469, 1509
XceI RCATGY 1 cut(s) 841
XmaI CCCGGG 1 cut(s) 1429
XmaJI CCTAGG 1 cut(s) 497
XmiI GTMKAC 2 cut(s) 81, 1098
XmnI GAANNNNTTC 1 cut(s) 1509
XspI CTAG 7 cut(s) 15, 345, 498, 567, 870, 1280, 1313
ZrmI AGTACT 1 cut(s) 13
Zsp2I ATGCAT 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.