Rorug04G0312800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016835
Physical Location & Seq
Forward (+)
48807237 .. 48813281
6045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0312800.1

Sequence Viewer

Length: 2424 bp
ATGGAAGGCTACAAGCCTCCGGCAGATTTTGAAGAGAATGCTAAAGACCCACTGCTCGATTTCAACGTGAACGATTCTACTGAGCTTTGGCTCATTCAGTGGCCTAAAAATCAAAATCCAGATTATGATGGGAAAGAGCTGTCGCTTCAGCTTCATGGTGATGGAAAGTTGGCCAGTTTTGAAGGCGCATCTGGGAAAGAATATGACCTGGTCAGTTCCTCAACTCAACAGTCAAATGCTACAGTTTTTGTATCTTCTGCCTCGGGCACAAAAATTGCTGGAAAGATTTCACGGCGAGTTTCTCTGGTGCATTTCCCCGAGCCTAGTGAGCTTAAAGAACGTCAAGAGCTTCAAATGGCAAAATTCCGAAAAAAGAATCAGATATCGTCCTCAATGGCATTGACCCATTCTTCCCAGAAAACTCCTTCACGCAGTACGAGAACTACAACTACTGCAACTCCTACTCCTACTCAAAGTACGTTTCAGAGAAGTTCGCGACCAATAAATGGACGCGCATCCTCCCCTCATAGTAGTGAACATAAAAGTTCTGTTAGTGAAGCTGGGGAGCAATCACAGCTTCGGAACAAACGGCATGCTTCCCCAGGTCGAAGTCATAGTGGCGTTACTTCATCAGGACAAGGTCGTAGTTCTACTACTACTTCCTCAGGAGAAAGACACTTGAAGCAGCATTACGATGTGTCAAAACTTTTTGCCGCCAAGCTTCATGATTTAATACGGTTTCCAGTTCAAACACCTCAGGCAAGGAGAAAGCATACATCCTTTGTTCATTCTACTGGGTCACATACTATCACGAAAATCTCCACCTGCCGACTGACGACAGACACCACCGTCGGTCCGAGTCCGACTGCTTCTGCAAATCTCCCGCTCTCGTCTATCGAAGAAGCTCATCGATCAGCTGCTCACAAAACTGCCAGGTCCCTGAAGTTGGGCCAATTCACAATGGATGTGGATACCAAGCTTCTAGACGGGGCTCTGCCGATTGTGGCAACTCTGGTATTAGCTGTCCTAATCGGAGTGATTATAATCCGGCGCAGATCTGGACACCGGCTTCCTCCTACTGTGAAGGCCTTGCCTTTGATTGGGGGGTTGCTTCGGTTCTTGAAAGGCCCCATTGTGATGATTAGGGAGGAGTACCCAAAGCTTGGGAGTGTGTTCACATTGAATCTGTTTCACAAGAAGATTACGTTCTTGATTGGTCCTGAGGTTTCCGCGCACTTTTTCAAGGCGCCGGAGTCTGATCTTAGCCAGCAGGAGGTGTACCAGTTCAATGTGCCCACTTTCGGACCCGGAGTTGTGTTTGATGTGGATTATACGGTGAGGCAGGAGCAGTTCAGGTTCTTTACGGAGGCTCTGAGGGTGAATAAGCTCAAGGGATATGTGGATCAGATGGTTACAGAAGCAGAGGATTACTTTTCCAAGTGGGGAGACAGTGGAGAGGTGGACTTGAAGTATGAGCTGGAGCATCTGATTATCCTGACAGCCAGTAGATGCCTCTTGGGTCGTGAAGTTCGTGATAAACTCTTTGATGATGTTTCGTCTTTGTTCCATGAACTTGACAATGGCATGCTTCCCATTAGTGTCATTGCCCCATACCTGCCCATCCCAGCTCACCGCCGCCGTGACCAGGCACGCAAGAAGCTCACAGCAATCTTTGCAAACATCATAAACTCCCGCAGATGTGCTGAAGTGTCTGAGAATGACATGTTGCAATATTTCATCGAGTCTAAGTACAAAAACGGCCGAAAAACAACTGATTCTGAGATCACTGGCTTGCTCATTGCTGCTCTCTTTGCTGGGCAACATACAAGTTCAATCACTTCCACTTGGACTGGGGCTTATCTCCTCCAGAATAAGCAGTACCTATCTGCCGTGTCAGAGGAACAGACAAGGCTTATGGCTAAGCATGGGAAGAAGATTGACCATGATATATTGTCCGAGATGGATACACTGTATCGTTCCATCAAGGAAGCTCTGAGACTGCATCCTCCACTGATCATGTTGCTGCGACACTCGCATAGCGATTTCAGCGTACAAACTCGTGACGGGAAAGAATATGACATCCCGAAGGGCCACATAGTTGCCACATCACCAGCTTTTGCCAACCGACTTCCTCATATTTTCAAAAACCCAGACAGCTATGATCCTGATAGATTTGCTGTTGGAAGAGAGGAAGACAAGGCCGCAGGGGCCTTCTCATATATCTCATTTGGAGGTGGCAGGCATGGATGCCTCGGAGAGCCTTTTGCATACCTGCAGATAAAGGCTATATGGAGCCATTTGCTGAGGAACTTTGAACTGGAGCTGGTTTCACCTTTTCCAGAGACTGATTGGAATGCCATGGTTGTCGGTGTGAAAGGAAAGGTGATGGTGCGGTATAAGCGAAGGGAGCTTTCAGCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

807

Amino Acids

90.18

Weight (kDa)

9.38

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 358 - 776 4.8e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016835)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30430
malus_domestica MD16G1063600.v1.1
prunus_persica Prupe.1G282900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0438721
rosa_laevigata RLG00000006334
rosa_multiflora Rmu_sc0000144.1_g000047 Rmu_sc0002505.1_g000006
rosa_roxburghii Rroxscaffold_5G00379760
rosa_rugosa Rorug04G0312800
rosa_samantha Rh4AG363400 Rh4BG374800 Rh4CG389000
rosa_wichuraiana Rw0G007190 Rw0G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1043
AarI CACCTGC 1 cut(s) 833
AasI GACNNNNNNGTC 1 cut(s) 848
Acc36I ACCTGC 3 cut(s) 833, 1623, 2280
AccB1I GGYRCC 1 cut(s) 1246
AccB7I CCANNNNNTGG 2 cut(s) 506, 1163
AccBSI CCGCTC 1 cut(s) 886
AccII CGCG 3 cut(s) 496, 513, 1232
AciI CCGC 8 cut(s) 714, 884, 1230, 1633, 1636, 1693, 2202, 2392
AclWI GGATC 2 cut(s) 1410, 2156
AcoI YGGCCR 2 cut(s) 171, 1759
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 3 cut(s) 131, 962, 1725
AcyI GRCGYC 1 cut(s) 1247
AfaI GTAC 7 cut(s) 436, 478, 1154, 1280, 1751, 1880, 2052
AfiI CCNNNNNNNGG 7 cut(s) 506, 946, 1100, 1163, 1273, 1301, 1645
AflIII ACRYGT 1 cut(s) 1722
AjnI CCWGG 4 cut(s) 207, 601, 932, 1644
Alw26I GTCTC 3 cut(s) 1440, 1990, 2336
AlwI GGATC 2 cut(s) 1410, 2156
AlwNI CAGNNNCTG 1 cut(s) 2345
Ama87I CYCGRG 2 cut(s) 262, 317
AoxI GGCC 9 cut(s) 101, 171, 949, 1086, 1126, 1759, 2089, 2199, 2208
ApeKI GCWGC 4 cut(s) 685, 917, 1802, 2023
ApoI RAATTY 1 cut(s) 362
Asp700I GAANNNNTTC 1 cut(s) 286
AspLEI GCGC 5 cut(s) 188, 515, 1053, 1234, 1249
AspS9I GGNCC 8 cut(s) 854, 936, 949, 1127, 1217, 1304, 2089, 2208
AsuC2I CCSGG 1 cut(s) 1308
AsuHPI GGTGA 7 cut(s) 170, 1348, 1390, 1622, 2100, 2322, 2395
AvaI CYCGRG 2 cut(s) 262, 317
AvaII GGWCC 4 cut(s) 854, 936, 1217, 1304
AxyI CCTNAGG 3 cut(s) 664, 756, 1221
BaeGI GKGCMC 2 cut(s) 269, 1296
BalI TGGCCA 1 cut(s) 173
BanI GGYRCC 1 cut(s) 1246
BanII GRGCYC 1 cut(s) 994
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BauI CACGAG 1 cut(s) 2058
BbsI GAAGAC 1 cut(s) 2199
BbvCI CCTCAGC 1 cut(s) 2303
BbvI GCAGC 4 cut(s) 697, 904, 1789, 2010
BccI CCATC 7 cut(s) 122, 155, 1402, 1628, 1954, 1988, 2380
BceAI ACGGC 5 cut(s) 308, 605, 1623, 1774, 1874
BciT130I CCWGG 4 cut(s) 209, 603, 934, 1646
BciVI GTATCC 2 cut(s) 964, 1957
BclI TGATCA 1 cut(s) 2013
BcnI CCSGG 1 cut(s) 1308
BcoDI GTCTC 3 cut(s) 1440, 1990, 2336
BfaI CTAG 2 cut(s) 324, 983
BfmI CTRYAG 2 cut(s) 240, 2273
BfoI RGCGCY 1 cut(s) 1250
BfuAI ACCTGC 3 cut(s) 833, 1623, 2280
BfuI GTATCC 2 cut(s) 964, 1957
BglI GCCNNNNNGGC 1 cut(s) 2207
BglII AGATCT 1 cut(s) 1055
BisI GCNGC 7 cut(s) 686, 714, 918, 1636, 1803, 2024, 2202
BlpI GCTNAGC 1 cut(s) 1920
BlsI GCNGC 7 cut(s) 687, 715, 919, 1637, 1804, 2025, 2203
Bme1390I CCNGG 5 cut(s) 209, 603, 934, 1308, 1646
Bme18I GGWCC 4 cut(s) 854, 936, 1217, 1304
BmeT110I CYCGRG 2 cut(s) 262, 317
BmgT120I GGNCC 8 cut(s) 854, 936, 949, 1127, 1217, 1304, 2089, 2208
BmiI GGNNCC 6 cut(s) 938, 1129, 1248, 1306, 2209, 2294
BmrFI CCNGG 5 cut(s) 209, 603, 934, 1308, 1646
BmrI ACTGGG 2 cut(s) 804, 1860
BmsI GCATC 6 cut(s) 197, 524, 1492, 1499, 2011, 2237
BmuI ACTGGG 2 cut(s) 804, 1860
BpiI GAAGAC 1 cut(s) 2199
BpmI CTGGAG 3 cut(s) 1499, 1850, 2339
Bpu10I CCTNAGC 1 cut(s) 2303
Bpu1102I GCTNAGC 1 cut(s) 1920
BpuEI CTTGAG 1 cut(s) 1373
BpuMI CCSGG 1 cut(s) 1308
Bsa29I ATCGAT 1 cut(s) 910
BsaBI GATNNNNATC 1 cut(s) 1043
BsaHI GRCGYC 1 cut(s) 1247
BsaJI CCNNGG 4 cut(s) 261, 601, 2251, 2358
BsaXI ACNNNNNCTCC 4 cut(s) 442, 448, 472, 478
Bsc4I CCNNNNNNNGG 7 cut(s) 506, 946, 1100, 1163, 1273, 1301, 1645
Bse118I RCCGGY 1 cut(s) 1065
Bse1I ACTGG 8 cut(s) 174, 743, 799, 1282, 1503, 1792, 1855, 2322
Bse21I CCTNAGG 3 cut(s) 664, 756, 1221
Bse3DI GCAATG 2 cut(s) 1602, 1797
Bse8I GATNNNNATC 1 cut(s) 1043
BseBI CCWGG 4 cut(s) 209, 603, 934, 1646
BseCI ATCGAT 1 cut(s) 910
BseDI CCNNGG 4 cut(s) 261, 601, 2251, 2358
BseGI GGATG 7 cut(s) 515, 776, 970, 1620, 2002, 2079, 2252
BseJI GATNNNNATC 1 cut(s) 1043
BseLI CCNNNNNNNGG 7 cut(s) 506, 946, 1100, 1163, 1273, 1301, 1645
BseMI GCAATG 2 cut(s) 1602, 1797
BseMII CTCAG 9 cut(s) 72, 678, 770, 1212, 1364, 1704, 1770, 1985, 2294
BseNI ACTGG 8 cut(s) 174, 743, 799, 1282, 1503, 1792, 1855, 2322
BseRI GAGGAG 2 cut(s) 1163, 1853
BseSI GKGCMC 2 cut(s) 269, 1296
BseX3I CGGCCG 1 cut(s) 1759
BseXI GCAGC 4 cut(s) 697, 904, 1789, 2010
BseYI CCCAGC 3 cut(s) 560, 1624, 1814
Bsh1236I CGCG 3 cut(s) 496, 513, 1232
Bsh1285I CGRYCG 1 cut(s) 1762
BshFI GGCC 9 cut(s) 103, 173, 951, 1088, 1128, 1761, 2091, 2201, 2210
BshNI GGYRCC 1 cut(s) 1246
BshVI ATCGAT 1 cut(s) 910
BsiEI CGRYCG 1 cut(s) 1762
BsiHKCI CYCGRG 2 cut(s) 262, 317
BsiSI CCGG 5 cut(s) 20, 1048, 1066, 1250, 1308
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 7 cut(s) 506, 946, 1100, 1163, 1273, 1301, 1645
BsmAI GTCTC 3 cut(s) 1440, 1990, 2336
BsmFI GGGAC 1 cut(s) 922
BsmI GAATGC 2 cut(s) 43, 2359
BsnI GGCC 9 cut(s) 103, 173, 951, 1088, 1128, 1761, 2091, 2201, 2210
BsoBI CYCGRG 2 cut(s) 262, 317
Bsp1286I GDGCHC 3 cut(s) 269, 994, 1296
Bsp143I GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
Bsp1720I GCTNAGC 1 cut(s) 1920
Bsp19I CCATGG 1 cut(s) 2358
Bsp68I TCGCGA 1 cut(s) 496
BspACI CCGC 8 cut(s) 714, 884, 1230, 1633, 1636, 1693, 2202, 2392
BspANI GGCC 9 cut(s) 103, 173, 951, 1088, 1128, 1761, 2091, 2201, 2210
BspCNI CTCAG 9 cut(s) 73, 677, 769, 1213, 1365, 1705, 1771, 1986, 2295
BspDI ATCGAT 1 cut(s) 910
BspFNI CGCG 3 cut(s) 496, 513, 1232
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 6 cut(s) 938, 1129, 1248, 1306, 2209, 2294
BspMAI CTGCAG 1 cut(s) 2277
BspMI ACCTGC 3 cut(s) 833, 1623, 2280
BspPI GGATC 2 cut(s) 1410, 2156
BspT107I GGYRCC 1 cut(s) 1246
BsrBI CCGCTC 1 cut(s) 886
BsrDI GCAATG 2 cut(s) 1602, 1797
BsrFI RCCGGY 1 cut(s) 1065
BsrI ACTGG 8 cut(s) 174, 743, 799, 1282, 1503, 1792, 1855, 2322
BssAI RCCGGY 1 cut(s) 1065
BssECI CCNNGG 4 cut(s) 261, 601, 2251, 2358
BssMI GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
BssNI GRCGYC 1 cut(s) 1247
BssSI CACGAG 1 cut(s) 2058
BssT1I CCWWGG 1 cut(s) 2358
Bst2BI CACGAG 1 cut(s) 2058
Bst2UI CCWGG 4 cut(s) 209, 603, 934, 1646
Bst4CI ACNGT 8 cut(s) 231, 244, 738, 850, 1081, 1336, 1451, 1971
Bst6I CTCTTC 2 cut(s) 27, 2179
BstACI GRCGYC 1 cut(s) 1247
BstAPI GCANNNNNTGC 1 cut(s) 1673
BstC8I GCNNGC 6 cut(s) 594, 1268, 1586, 1651, 1793, 2240
BstDSI CCRYGG 1 cut(s) 2358
BstF5I GGATG 7 cut(s) 515, 776, 970, 1620, 2002, 2079, 2252
BstFNI CGCG 3 cut(s) 496, 513, 1232
BstH2I RGCGCY 1 cut(s) 1250
BstHHI GCGC 5 cut(s) 188, 515, 1053, 1234, 1249
BstKTI GATC 7 cut(s) 914, 1058, 1261, 1405, 1785, 2016, 2164
BstMAI GTCTC 3 cut(s) 1440, 1990, 2336
BstMBI GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
BstMCI CGRYCG 1 cut(s) 1762
BstMWI GCNNNNNNNGC 9 cut(s) 328, 574, 1673, 1811, 2032, 2046, 2207, 2398, 2407
BstNI CCWGG 4 cut(s) 209, 603, 934, 1646
BstNSI RCATGY 3 cut(s) 596, 1588, 1726
BstSCI CCNGG 5 cut(s) 207, 601, 932, 1306, 1644
BstSFI CTRYAG 2 cut(s) 240, 2273
BstSLI GKGCMC 2 cut(s) 269, 1296
BstUI CGCG 3 cut(s) 496, 513, 1232
BstV1I GCAGC 4 cut(s) 697, 904, 1789, 2010
BstV2I GAAGAC 1 cut(s) 2199
BstX2I RGATCY 1 cut(s) 1055
BstYI RGATCY 1 cut(s) 1055
BstZI CGGCCG 1 cut(s) 1759
Bsu15I ATCGAT 1 cut(s) 910
Bsu36I CCTNAGG 3 cut(s) 664, 756, 1221
BsuI GTATCC 2 cut(s) 964, 1957
BsuRI GGCC 9 cut(s) 103, 173, 951, 1088, 1128, 1761, 2091, 2201, 2210
BsuTUI ATCGAT 1 cut(s) 910
BtgI CCRYGG 1 cut(s) 2358
BtsCI GGATG 7 cut(s) 515, 776, 970, 1620, 2002, 2079, 2252
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 6 cut(s) 50, 104, 1456, 1785, 1967, 2009
BtuMI TCGCGA 1 cut(s) 496
BveI ACCTGC 3 cut(s) 833, 1623, 2280
Cac8I GCNNGC 6 cut(s) 594, 1268, 1586, 1651, 1793, 2240
CaiI CAGNNNCTG 1 cut(s) 2345
CciI TCATGA 1 cut(s) 724
CfoI GCGC 5 cut(s) 188, 515, 1053, 1234, 1249
Cfr10I RCCGGY 1 cut(s) 1065
Cfr13I GGNCC 8 cut(s) 854, 936, 949, 1127, 1217, 1304, 2089, 2208
ClaI ATCGAT 1 cut(s) 910
CpoI CGGWCCG 1 cut(s) 854
CseI GACGC 1 cut(s) 519
CsiI ACCWGGT 1 cut(s) 207
Csp6I GTAC 7 cut(s) 435, 477, 1153, 1279, 1750, 1879, 2051
CspCI CAANNNNNGTGG 2 cut(s) 948, 983
CspI CGGWCCG 1 cut(s) 854
CviQI GTAC 7 cut(s) 435, 477, 1153, 1279, 1750, 1879, 2051
DinI GGCGCC 1 cut(s) 1248
DpnI GATC 7 cut(s) 913, 1057, 1260, 1404, 1784, 2015, 2163
DpnII GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
EaeI YGGCCR 2 cut(s) 171, 1759
EagI CGGCCG 1 cut(s) 1759
Eam1104I CTCTTC 2 cut(s) 27, 2179
EarI CTCTTC 2 cut(s) 27, 2179
EclXI CGGCCG 1 cut(s) 1759
Eco130I CCWWGG 1 cut(s) 2358
Eco147I AGGCCT 1 cut(s) 1088
Eco24I GRGCYC 1 cut(s) 994
Eco32I GATATC 1 cut(s) 384
Eco47I GGWCC 4 cut(s) 854, 936, 1217, 1304
Eco52I CGGCCG 1 cut(s) 1759
Eco57I CTGAAG 3 cut(s) 131, 962, 1725
Eco81I CCTNAGG 3 cut(s) 664, 756, 1221
Eco88I CYCGRG 2 cut(s) 262, 317
EcoO109I RGGNCCY 3 cut(s) 936, 1127, 2208
EcoRII CCWGG 4 cut(s) 207, 601, 932, 1644
EcoRV GATATC 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 2358
EcoT38I GRGCYC 1 cut(s) 994
EgeI GGCGCC 1 cut(s) 1248
EheI GGCGCC 1 cut(s) 1248
ErhI CCWWGG 1 cut(s) 2358
FaqI GGGAC 1 cut(s) 922
FauI CCCGC 2 cut(s) 891, 1700
FbaI TGATCA 1 cut(s) 2013
Fnu4HI GCNGC 7 cut(s) 686, 714, 918, 1636, 1803, 2024, 2202
FokI GGATG 7 cut(s) 502, 763, 977, 1607, 1989, 2066, 2259
FriOI GRGCYC 1 cut(s) 994
Fsp4HI GCNGC 7 cut(s) 686, 714, 918, 1636, 1803, 2024, 2202
FspBI CTAG 2 cut(s) 324, 983
GlaI GCGC 5 cut(s) 187, 514, 1052, 1233, 1248
GluI GCNGC 7 cut(s) 686, 714, 918, 1636, 1803, 2024, 2202
GsaI CCCAGC 3 cut(s) 564, 1628, 1818
GsuI CTGGAG 3 cut(s) 1499, 1850, 2339
HaeII RGCGCY 1 cut(s) 1250
HaeIII GGCC 9 cut(s) 103, 173, 951, 1088, 1128, 1761, 2091, 2201, 2210
HapII CCGG 5 cut(s) 20, 1048, 1066, 1250, 1308
HgaI GACGC 1 cut(s) 519
HhaI GCGC 5 cut(s) 188, 515, 1053, 1234, 1249
Hin1I GRCGYC 1 cut(s) 1247
Hin6I GCGC 5 cut(s) 186, 513, 1051, 1232, 1247
HinP1I GCGC 5 cut(s) 186, 513, 1051, 1232, 1247
HindIII AAGCTT 3 cut(s) 719, 977, 1160
HinfI GANTC 7 cut(s) 74, 376, 859, 1183, 1253, 1742, 1775
HpaII CCGG 5 cut(s) 20, 1048, 1066, 1250, 1308
HphI GGTGA 7 cut(s) 170, 1348, 1390, 1622, 2100, 2322, 2395
Hpy166II GTNNAC 5 cut(s) 70, 536, 1176, 1279, 1462
Hpy8I GTNNAC 5 cut(s) 70, 536, 1176, 1279, 1462
Hpy99I CGWCG 1 cut(s) 854
HpyAV CCTTC 6 cut(s) 176, 435, 1078, 2080, 2221, 2397
HpyCH4III ACNGT 8 cut(s) 231, 244, 738, 850, 1081, 1336, 1451, 1971
HpyCH4IV ACGT 4 cut(s) 66, 340, 479, 1205
HpyCH4V TGCA 8 cut(s) 310, 455, 875, 1676, 1729, 2002, 2267, 2275
HpyF10VI GCNNNNNNNGC 9 cut(s) 328, 574, 1673, 1811, 2032, 2046, 2207, 2398, 2407
HpySE526I ACGT 4 cut(s) 66, 340, 479, 1205
Hsp92I GRCGYC 1 cut(s) 1247
HspAI GCGC 5 cut(s) 186, 513, 1051, 1232, 1247
KasI GGCGCC 1 cut(s) 1246
Ksp22I TGATCA 1 cut(s) 2013
Kzo9I GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
LmnI GCTCC 6 cut(s) 565, 1345, 1480, 2292, 2320, 2407
Lsp1109I GCAGC 4 cut(s) 697, 904, 1789, 2010
LweI GCATC 6 cut(s) 197, 524, 1492, 1499, 2011, 2237
MabI ACCWGGT 1 cut(s) 207
MaeI CTAG 2 cut(s) 324, 983
MaeII ACGT 4 cut(s) 66, 340, 479, 1205
MaeIII GTNAC 5 cut(s) 622, 798, 1411, 1640, 2060
MalI GATC 7 cut(s) 913, 1057, 1260, 1404, 1784, 2015, 2163
MbiI CCGCTC 1 cut(s) 886
MboI GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
MboII GAAGA 9 cut(s) 44, 246, 402, 911, 1210, 1942, 1945, 2196, 2204
MflI RGATCY 1 cut(s) 1055
MhlI GDGCHC 3 cut(s) 269, 994, 1296
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 4 cut(s) 273, 362, 953, 2419
MluNI TGGCCA 1 cut(s) 173
Mly113I GGCGCC 1 cut(s) 1247
MlyI GAGTC 3 cut(s) 868, 1262, 1751
MmeI TCCRAC 2 cut(s) 887, 2161
Mox20I TGGCCA 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 286
MscI TGGCCA 1 cut(s) 173
MseI TTAA 3 cut(s) 333, 731, 2422
MslI CAYNNNNRTG 4 cut(s) 159, 531, 693, 1136
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 5 cut(s) 20, 1048, 1066, 1250, 1308
MspR9I CCNGG 5 cut(s) 209, 603, 934, 1308, 1646
Mva1269I GAATGC 2 cut(s) 43, 2359
MvaI CCWGG 4 cut(s) 209, 603, 934, 1646
MvnI CGCG 3 cut(s) 496, 513, 1232
MwoI GCNNNNNNNGC 9 cut(s) 328, 574, 1673, 1811, 2032, 2046, 2207, 2398, 2407
NarI GGCGCC 1 cut(s) 1247
NciI CCSGG 1 cut(s) 1308
NcoI CCATGG 1 cut(s) 2358
NdeII GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
NlaIV GGNNCC 6 cut(s) 938, 1129, 1248, 1306, 2209, 2294
NmuCI GTSAC 3 cut(s) 798, 1640, 2060
NruI TCGCGA 1 cut(s) 496
NspI RCATGY 3 cut(s) 596, 1588, 1726
PaeI GCATGC 2 cut(s) 596, 1588
PagI TCATGA 1 cut(s) 724
PaqCI CACCTGC 1 cut(s) 833
PceI AGGCCT 1 cut(s) 1088
PciI ACATGT 1 cut(s) 1722
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 1528
PctI GAATGC 2 cut(s) 43, 2359
PdmI GAANNNNTTC 1 cut(s) 286
PfeI GAWTC 4 cut(s) 74, 376, 1183, 1775
PflFI GACNNNGTC 2 cut(s) 209, 639
PflMI CCANNNNNTGG 2 cut(s) 506, 1163
PkrI GCNGC 7 cut(s) 687, 715, 919, 1637, 1804, 2025, 2203
PleI GAGTC 3 cut(s) 867, 1261, 1750
PluTI GGCGCC 1 cut(s) 1250
PpsI GAGTC 3 cut(s) 867, 1261, 1750
PpuMI RGGWCCY 1 cut(s) 936
PscI ACATGT 1 cut(s) 1722
PsiI TTATAA 1 cut(s) 1043
Psp5II RGGWCCY 1 cut(s) 936
Psp6I CCWGG 4 cut(s) 207, 601, 932, 1644
PspFI CCCAGC 3 cut(s) 560, 1624, 1814
PspGI CCWGG 4 cut(s) 207, 601, 932, 1644
PspN4I GGNNCC 6 cut(s) 938, 1129, 1248, 1306, 2209, 2294
PspPI GGNCC 8 cut(s) 854, 936, 949, 1127, 1217, 1304, 2089, 2208
PspPPI RGGWCCY 1 cut(s) 936
PsrI GAACNNNNNNTAC 2 cut(s) 433, 465
PstI CTGCAG 1 cut(s) 2277
PstNI CAGNNNCTG 1 cut(s) 2345
PsuI RGATCY 1 cut(s) 1055
PsyI GACNNNGTC 2 cut(s) 209, 639
PvuII CAGCTG 1 cut(s) 917
RruI TCGCGA 1 cut(s) 496
RsaI GTAC 7 cut(s) 436, 478, 1154, 1280, 1751, 1880, 2052
RsaNI GTAC 7 cut(s) 435, 477, 1153, 1279, 1750, 1879, 2051
RseI CAYNNNNRTG 4 cut(s) 159, 531, 693, 1136
Rsr2I CGGWCCG 1 cut(s) 854
RsrII CGGWCCG 1 cut(s) 854
SaqAI TTAA 3 cut(s) 333, 731, 2422
SatI GCNGC 7 cut(s) 686, 714, 918, 1636, 1803, 2024, 2202
Sau3AI GATC 7 cut(s) 911, 1055, 1258, 1402, 1782, 2013, 2161
Sau96I GGNCC 8 cut(s) 854, 936, 949, 1127, 1217, 1304, 2089, 2208
SchI GAGTC 3 cut(s) 868, 1262, 1751
ScrFI CCNGG 5 cut(s) 209, 603, 934, 1308, 1646
SduI GDGCHC 3 cut(s) 269, 994, 1296
SexAI ACCWGGT 1 cut(s) 207
SfaNI GCATC 6 cut(s) 197, 524, 1492, 1499, 2011, 2237
SfcI CTRYAG 2 cut(s) 240, 2273
SfiI GGCCNNNNNGGCC 1 cut(s) 2207
SfoI GGCGCC 1 cut(s) 1248
SinI GGWCC 4 cut(s) 854, 936, 1217, 1304
SmiMI CAYNNNNRTG 4 cut(s) 159, 531, 693, 1136
SmlI CTYRAG 1 cut(s) 1388
SmoI CTYRAG 1 cut(s) 1388
SphI GCATGC 2 cut(s) 596, 1588
Sse9I AATT 4 cut(s) 273, 362, 953, 2419
SseBI AGGCCT 1 cut(s) 1088
SsiI CCGC 8 cut(s) 714, 884, 1230, 1633, 1636, 1693, 2202, 2392
SspDI GGCGCC 1 cut(s) 1246
SspI AATATT 1 cut(s) 1733
SspMI CTAG 2 cut(s) 324, 983
StuI AGGCCT 1 cut(s) 1088
StyD4I CCNGG 5 cut(s) 207, 601, 932, 1306, 1644
StyI CCWWGG 1 cut(s) 2358
TaaI ACNGT 8 cut(s) 231, 244, 738, 850, 1081, 1336, 1451, 1971
TaiI ACGT 4 cut(s) 69, 343, 482, 1208
TaqI TCGA 5 cut(s) 57, 607, 897, 910, 1740
TaqII GACCGA 1 cut(s) 842
TasI AATT 4 cut(s) 273, 362, 953, 2419
TatI WGTACW 1 cut(s) 1749
TauI GCSGC 3 cut(s) 716, 1638, 2204
TfiI GAWTC 4 cut(s) 74, 376, 1183, 1775
Tru1I TTAA 3 cut(s) 333, 731, 2422
Tru9I TTAA 3 cut(s) 333, 731, 2422
TscAI CASTG 6 cut(s) 57, 104, 1456, 1792, 1974, 2016
TseFI GTSAC 3 cut(s) 798, 1640, 2060
TseI GCWGC 4 cut(s) 685, 917, 1802, 2023
Tsp45I GTSAC 3 cut(s) 798, 1640, 2060
TspDTI ATGAA 6 cut(s) 143, 618, 713, 776, 1584, 1726
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 6 cut(s) 57, 104, 1456, 1792, 1974, 2016
Tth111I GACNNNGTC 2 cut(s) 209, 639
Van91I CCANNNNNTGG 2 cut(s) 506, 1163
VpaK11BI GGWCC 4 cut(s) 854, 936, 1217, 1304
XapI RAATTY 1 cut(s) 362
XbaI TCTAGA 1 cut(s) 982
XceI RCATGY 3 cut(s) 596, 1588, 1726
XcmI CCANNNNNNNNNTGG 1 cut(s) 2346
XmnI GAANNNNTTC 1 cut(s) 286
XspI CTAG 2 cut(s) 324, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.