RchiOBHm_Chr5g0006071

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019941
Physical Location & Seq
Forward (+)
3814348 .. 3814831
484 bp
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UTR
Exon/CDS
Intron
PRQ28722

Sequence Viewer

Length: 285 bp
ATGCAGAGTCATGATCAAGACGCGCTTTTTGGAGCGGAAAAGAAAGACATTCCTCTTGATTTTAGCACCATGAGCTACACGCATTTGGTGGAAATCCTCGTCCGCTACTTGCAGCAAAAGAGGTACGTGGTTGTCTTAGATGATGTGTGGGATATAGATCTTTGGAAACAAATACATGTCGCATTTCCGGATGGAAGAAATGGAAGTCGGGTCATGCTTGCAACTCGAAAAGAAGACGTTGCTTCTTTTTCATTTGGAGTTGAAAAGTTATCTTCACCATGTTGA

Protein Analysis

94

Amino Acids

10.83

Weight (kDa)

5.47

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 22 - 84 1.5e-18 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019941)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0006061 RchiOBHm_Chr5g0006071
rosa_multiflora Rmu_sc0007990.1_g000001
rosa_samantha Rh5AG342600 Rh5AG342900 Rh5AG343300 Rh6DG009800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 2 cut(s) 35, 103
AfaI GTAC 1 cut(s) 125
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 1 cut(s) 263
AluBI AGCT 1 cut(s) 75
AluI AGCT 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 187
ApeKI GCWGC 1 cut(s) 112
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 124
BccI CCATC 1 cut(s) 185
BclI TGATCA 1 cut(s) 13
BglII AGATCT 1 cut(s) 157
BisI GCNGC 1 cut(s) 113
BlsI GCNGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 127
BsaBI GATNNNNATC 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 187
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 156
BseXI GCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 187
Bsp143I GATC 2 cut(s) 13, 157
BspACI CCGC 2 cut(s) 35, 103
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 35
BssMI GATC 2 cut(s) 13, 157
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 136
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 2 cut(s) 16, 160
BstMBI GATC 2 cut(s) 13, 157
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 179
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BtsCI GGATG 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 219
CciI TCATGA 1 cut(s) 10
CfoI GCGC 1 cut(s) 25
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 124
CviAII CATG 5 cut(s) 11, 70, 176, 214, 279
CviJI RGCY 1 cut(s) 75
CviKI_1 RGCY 1 cut(s) 75
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 15, 159
DpnII GATC 2 cut(s) 13, 157
FaeI CATG 5 cut(s) 14, 73, 179, 217, 282
FaiI YATR 6 cut(s) 12, 71, 155, 177, 215, 280
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 5 cut(s) 10, 69, 175, 213, 278
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 113
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 113
GlaI GCGC 1 cut(s) 24
GluI GCNGC 1 cut(s) 113
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 25
Hin1II CATG 5 cut(s) 14, 73, 179, 217, 282
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 188
HphI GGTGA 1 cut(s) 267
Hpy188III TCNNGA 4 cut(s) 11, 17, 56, 188
HpyCH4IV ACGT 2 cut(s) 126, 237
HpyCH4V TGCA 3 cut(s) 4, 112, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 2 cut(s) 126, 237
Hsp92II CATG 5 cut(s) 14, 73, 179, 217, 282
HspAI GCGC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 2 cut(s) 13, 157
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 1 cut(s) 201
Lsp1109I GCAGC 1 cut(s) 124
MaeII ACGT 2 cut(s) 126, 237
MalI GATC 2 cut(s) 15, 159
MbiI CCGCTC 1 cut(s) 35
MboI GATC 2 cut(s) 13, 157
MboII GAAGA 3 cut(s) 207, 245, 264
MflI RGATCY 1 cut(s) 157
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 3 cut(s) 63, 107, 114
MroI TCCGGA 1 cut(s) 187
MspI CCGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 72
NdeII GATC 2 cut(s) 13, 157
NlaIII CATG 5 cut(s) 14, 73, 179, 217, 282
NspI RCATGY 1 cut(s) 179
PagI TCATGA 1 cut(s) 10
PciI ACATGT 1 cut(s) 175
PkrI GCNGC 1 cut(s) 114
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 127
PscI ACATGT 1 cut(s) 175
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 1 cut(s) 113
Sau3AI GATC 2 cut(s) 13, 157
SchI GAGTC 1 cut(s) 16
SetI ASST 4 cut(s) 77, 125, 129, 240
SsiI CCGC 2 cut(s) 35, 103
TaiI ACGT 2 cut(s) 129, 240
TaqI TCGA 1 cut(s) 226
TseI GCWGC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 240
XceI RCATGY 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.