RchiOBHm_Chr5g0018121

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020542
Physical Location & Seq
Reverse (-)
12632559 .. 12632967
409 bp
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UTR
Exon/CDS
Intron
PRQ29834

Sequence Viewer

Length: 315 bp
ATGCAAGTCAATTCAGTAGCCGAATACCTCACTCTGGTTCAGATCGAAGGAGTTAACTGTCAGGCTGAAGCTGTGTGGTATGCTGGTAAGCGCATGGCCTTTTTCTACAGGGCTAAGGTGATGAAAAATGGGAGCTATTACCACTGCATTTGGGGAAAGGTTTCCAGGCCTCATCGTAACAGTGGTACTGTTCGTGCAAAGTTCAAGTCCAGTTTGCCTCCCAAATACTTGGGTGTAAGGATGAGGGTGTTTATGTATCCCATGCCAGCAATATCTAAGCTAGGTACTTGTTCTTTACTTTGTTCGACATGTTGA

Protein Analysis

104

Amino Acids

11.81

Weight (kDa)

9.89

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35Ae PF01247 8 - 84 1.3e-24 Ribosomal protein L35Ae
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020542)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0018121
rosa_multiflora Rmu_sc0010280.1_g000023
rosa_samantha Rh5BG130000 Rh5CG144000 Rh5DG133800
rosa_wichuraiana Rw5G011620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 2 cut(s) 187, 286
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 3 cut(s) 71, 135, 280
AluI AGCT 3 cut(s) 71, 135, 280
AoxI GGCC 2 cut(s) 96, 167
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 160
AspLEI GCGC 1 cut(s) 93
AsuHPI GGTGA 1 cut(s) 130
BciT130I CCWGG 1 cut(s) 166
BciVI GTATCC 1 cut(s) 267
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 106
BfuI GTATCC 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 166
BmrFI CCNGG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 166
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 210
BshFI GGCC 2 cut(s) 98, 169
BslI CCNNNNNNNGG 1 cut(s) 34
BsnI GGCC 2 cut(s) 98, 169
Bsp143I GATC 1 cut(s) 42
BspANI GGCC 2 cut(s) 98, 169
BsrI ACTGG 1 cut(s) 210
BssMI GATC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 3 cut(s) 59, 182, 190
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 2 cut(s) 114, 276
BstF5I GGATG 1 cut(s) 246
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 106
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 2 cut(s) 98, 169
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 2 cut(s) 142, 187
Cac8I GCNNGC 1 cut(s) 267
CfoI GCGC 1 cut(s) 93
Csp6I GTAC 2 cut(s) 186, 285
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviAII CATG 3 cut(s) 94, 262, 309
CviJI RGCY 8 cut(s) 20, 65, 71, 98, 113, 135, 169, 280
CviKI_1 RGCY 8 cut(s) 20, 65, 71, 98, 113, 135, 169, 280
CviQI GTAC 2 cut(s) 186, 285
DdeI CTNAG 2 cut(s) 114, 276
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 164
FaeI CATG 3 cut(s) 97, 265, 312
FaiI YATR 5 cut(s) 81, 95, 254, 263, 310
FatI CATG 3 cut(s) 93, 261, 308
FokI GGATG 1 cut(s) 253
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 92
HaeIII GGCC 2 cut(s) 98, 169
HhaI GCGC 1 cut(s) 93
Hin1II CATG 3 cut(s) 97, 265, 312
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HpaI GTTAAC 1 cut(s) 55
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 42
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 59, 182, 190
HpyCH4V TGCA 3 cut(s) 4, 147, 197
HpyF3I CTNAG 2 cut(s) 114, 276
Hsp92II CATG 3 cut(s) 97, 265, 312
HspAI GCGC 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 1 cut(s) 132
LpnPI CCDG 8 cut(s) 20, 47, 69, 94, 151, 178, 223, 279
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MluCI AATT 1 cut(s) 10
MnlI CCTC 4 cut(s) 38, 180, 228, 237
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 166
MvaI CCWGG 1 cut(s) 166
NdeII GATC 1 cut(s) 42
NlaIII CATG 3 cut(s) 97, 265, 312
NspI RCATGY 1 cut(s) 312
PceI AGGCCT 1 cut(s) 169
PciI ACATGT 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 160
PscI ACATGT 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
RsaI GTAC 2 cut(s) 187, 286
RsaNI GTAC 2 cut(s) 186, 285
SaqAI TTAA 1 cut(s) 54
Sau3AI GATC 1 cut(s) 42
ScrFI CCNGG 1 cut(s) 166
SetI ASST 7 cut(s) 30, 73, 120, 137, 162, 282, 286
SfcI CTRYAG 1 cut(s) 106
Sse9I AATT 1 cut(s) 10
SseBI AGGCCT 1 cut(s) 169
SspMI CTAG 1 cut(s) 281
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 164
TaaI ACNGT 3 cut(s) 59, 182, 190
TaqI TCGA 2 cut(s) 45, 305
TasI AATT 1 cut(s) 10
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 2 cut(s) 149, 187
TspDTI ATGAA 1 cut(s) 137
TspRI CASTG 2 cut(s) 149, 187
XceI RCATGY 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.