RchiOBHm_Chr5g0035591

Phosphoenolpyruvate carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020496
Physical Location & Seq
Forward (+)
29575758 .. 29580377
4620 bp
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UTR
Exon/CDS
Intron
PRQ31444

Sequence Viewer

Length: 852 bp
ATGTGTGCTAGAGATACTTCTGTATGGCAAACAGATGAGCTTAGGCACCATAAACCCACACCAGGTGATGAAGCTAGGGCAGGTTTGAACATTGTGGAGCAGTCCCTTTGGAAAGCTGTACCTCATTATTTACGTCGTGTCAGCGATGCTTTAAAGAAGGGAGGGGGGTGTATTTGGTTTGATCGAGATGGAAATTCCAATGTGACAGCAAAGGTACTCGTGGGTCTTATTGATATTTTATTCGTAAATCTTCTCCTAATGGTATTATTTACTCAAATCTTGGAGAGGCCTTTCCTGTCAACAGAAGACCTGAAGCATGAACTAATCATAACTTTGGTATATATTAGATCTCTGTTATACATCCTTAGTTGTGTAAAGTGGATGACTATTGATCTCTATATTCGAGAAGTGGATAGCCTGAAATTTGAACTATCCTTGAATAGGTGCAGCGATATCTTGTCAAAACTGGCACAAGAGATTCTAGAACCCGAATCTGCATCTGACGATCACCAGAGTTGGGATCATCAGTGTTATAATAGGAATCAGGTAAAGCAACAAGCTGTAGCACTTCCAACACAACTTCCAGCTAGAGCTGATCAACCCTGTTGCACTGGTGAACATCCGATTATCCTCTTAAAATTGGGCACACACAGTGAGTGGGATGAAGAAAAGAAACTGGAATTTCTAACAAGAGAGCTCAAAGGAAAAAGACCGCTAGTCCCACTTAGCATGGAGGCAAGTGACATCCTTGCTGTGGAGCTTTTGCAAAAAGATGCACGACTTGCCGTTAGTGGAGAGATAGGAAAGCCGTGTCCTGGTGGAACGGAAGCTGCGTGTGGTTCCTTTGTTTGA

Protein Analysis

283

Amino Acids

31.89

Weight (kDa)

5.66

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPcase PF00311 7 - 74 1.9e-12 Phosphoenolpyruvate carboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 45
AciI CCGC 1 cut(s) 713
AclWI GGATC 1 cut(s) 528
AcsI RAATTY 3 cut(s) 193, 422, 680
AcuI CTGAAG 1 cut(s) 332
AdeI CACNNNGTG 2 cut(s) 65, 653
AfaI GTAC 2 cut(s) 120, 216
AfiI CCNNNNNNNGG 5 cut(s) 62, 441, 517, 754, 815
AgsI TTSAA 3 cut(s) 88, 428, 439
AhdI GACNNNNNGTC 1 cut(s) 716
AjnI CCWGG 2 cut(s) 61, 814
AluBI AGCT 9 cut(s) 40, 74, 116, 560, 587, 593, 697, 760, 830
AluI AGCT 9 cut(s) 40, 74, 116, 560, 587, 593, 697, 760, 830
Alw21I GWGCWC 1 cut(s) 699
AlwI GGATC 1 cut(s) 528
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 2 cut(s) 447, 830
ApoI RAATTY 3 cut(s) 193, 422, 680
AsuHPI GGTGA 3 cut(s) 77, 500, 626
BaeGI GKGCMC 1 cut(s) 647
BaeI ACNNNNGTAYC 1 cut(s) 39
BanI GGYRCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 699
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 2 cut(s) 459, 817
BccI CCATC 1 cut(s) 182
BceAI ACGGC 2 cut(s) 770, 793
BciT130I CCWGG 2 cut(s) 63, 816
BclI TGATCA 1 cut(s) 595
BfaI CTAG 5 cut(s) 9, 75, 482, 588, 716
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 71
BglII AGATCT 1 cut(s) 347
BisI GCNGC 2 cut(s) 448, 831
BlsI GCNGC 2 cut(s) 449, 832
Bme1390I CCNGG 2 cut(s) 63, 816
BmeRI GACNNNNNGTC 1 cut(s) 716
BmiI GGNNCC 2 cut(s) 47, 841
BmrFI CCNGG 2 cut(s) 63, 816
BmsI GCATC 3 cut(s) 136, 506, 763
BpiI GAAGAC 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 41
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 441, 517, 754, 815
Bse1I ACTGG 3 cut(s) 471, 616, 681
BseBI CCWGG 2 cut(s) 63, 816
BseGI GGATG 5 cut(s) 360, 387, 619, 667, 744
BseLI CCNNNNNNNGG 5 cut(s) 62, 441, 517, 754, 815
BseNI ACTGG 3 cut(s) 471, 616, 681
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 2 cut(s) 459, 817
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 1 cut(s) 289
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 699
BslFI GGGAC 2 cut(s) 88, 704
BslI CCNNNNNNNGG 5 cut(s) 62, 441, 517, 754, 815
BsmFI GGGAC 2 cut(s) 88, 704
BsnI GGCC 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 647, 699
Bsp143I GATC 6 cut(s) 181, 347, 391, 505, 520, 595
BspACI CCGC 1 cut(s) 713
BspANI GGCC 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 47, 841
BspMI ACCTGC 1 cut(s) 71
BspPI GGATC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 45
BsrI ACTGG 3 cut(s) 471, 616, 681
BssMI GATC 6 cut(s) 181, 347, 391, 505, 520, 595
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 63, 816
Bst4CI ACNGT 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 782
BstDEI CTNAG 3 cut(s) 41, 365, 725
BstENI CCTNNNNNAGG 1 cut(s) 439
BstF5I GGATG 5 cut(s) 360, 387, 619, 667, 744
BstKTI GATC 6 cut(s) 184, 350, 394, 508, 523, 598
BstMBI GATC 6 cut(s) 181, 347, 391, 505, 520, 595
BstMWI GCNNNNNNNGC 1 cut(s) 782
BstNI CCWGG 2 cut(s) 63, 816
BstSCI CCNGG 2 cut(s) 61, 814
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 647
BstV1I GCAGC 2 cut(s) 459, 817
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
BsuRI GGCC 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 5 cut(s) 360, 387, 619, 667, 744
BtsIMutI CAGTG 3 cut(s) 533, 609, 658
BveI ACCTGC 1 cut(s) 71
CsiI ACCWGGT 1 cut(s) 61
Csp6I GTAC 2 cut(s) 119, 215
CviAII CATG 2 cut(s) 317, 730
CviQI GTAC 2 cut(s) 119, 215
DdeI CTNAG 3 cut(s) 41, 365, 725
DpnI GATC 6 cut(s) 183, 349, 393, 507, 522, 597
DpnII GATC 6 cut(s) 181, 347, 391, 505, 520, 595
DraI TTTAAA 1 cut(s) 153
DraIII CACNNNGTG 2 cut(s) 65, 653
DriI GACNNNNNGTC 1 cut(s) 716
Eam1105I GACNNNNNGTC 1 cut(s) 716
Ecl136II GAGCTC 1 cut(s) 697
Eco147I AGGCCT 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 454
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 61, 814
EcoRV GATATC 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 699
FaeI CATG 2 cut(s) 320, 733
FaqI GGGAC 2 cut(s) 88, 704
FatI CATG 2 cut(s) 316, 729
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 2 cut(s) 448, 831
FokI GGATG 5 cut(s) 347, 394, 606, 674, 731
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 2 cut(s) 448, 831
FspBI CTAG 5 cut(s) 9, 75, 482, 588, 716
GluI GCNGC 2 cut(s) 448, 831
HaeIII GGCC 1 cut(s) 289
Hin1II CATG 2 cut(s) 320, 733
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 3 cut(s) 478, 491, 541
HphI GGTGA 3 cut(s) 77, 500, 626
Hpy166II GTNNAC 2 cut(s) 300, 617
Hpy188I TCNGA 2 cut(s) 502, 624
Hpy188III TCNNGA 3 cut(s) 185, 404, 482
Hpy8I GTNNAC 2 cut(s) 300, 617
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 653
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 5 cut(s) 447, 497, 609, 766, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 782
HpyF3I CTNAG 3 cut(s) 41, 365, 725
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 2 cut(s) 320, 733
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 181, 347, 391, 505, 520, 595
LmnI GCTCC 2 cut(s) 97, 757
Lsp1109I GCAGC 2 cut(s) 459, 817
LweI GCATC 3 cut(s) 136, 506, 763
MabI ACCWGGT 1 cut(s) 61
MaeI CTAG 5 cut(s) 9, 75, 482, 588, 716
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 202, 740
MalI GATC 6 cut(s) 183, 349, 393, 507, 522, 597
MboI GATC 6 cut(s) 181, 347, 391, 505, 520, 595
MboII GAAGA 3 cut(s) 242, 317, 677
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 2 cut(s) 647, 699
MluCI AATT 4 cut(s) 193, 422, 638, 680
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 5 cut(s) 132, 155, 279, 641, 727
MseI TTAA 2 cut(s) 152, 635
MspR9I CCNGG 2 cut(s) 63, 816
MvaI CCWGG 2 cut(s) 63, 816
MwoI GCNNNNNNNGC 1 cut(s) 782
NdeII GATC 6 cut(s) 181, 347, 391, 505, 520, 595
NlaIII CATG 2 cut(s) 320, 733
NlaIV GGNNCC 2 cut(s) 47, 841
NmuCI GTSAC 2 cut(s) 202, 740
PceI AGGCCT 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 830
PfeI GAWTC 3 cut(s) 478, 491, 541
PkrI GCNGC 2 cut(s) 449, 832
PsiI TTATAA 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 61, 814
PspGI CCWGG 2 cut(s) 61, 814
PspN4I GGNNCC 2 cut(s) 47, 841
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 2 cut(s) 120, 216
RsaNI GTAC 2 cut(s) 119, 215
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 2 cut(s) 152, 635
SatI GCNGC 2 cut(s) 448, 831
Sau3AI GATC 6 cut(s) 181, 347, 391, 505, 520, 595
ScrFI CCNGG 2 cut(s) 63, 816
SduI GDGCHC 2 cut(s) 647, 699
SexAI ACCWGGT 1 cut(s) 61
SfaNI GCATC 3 cut(s) 136, 506, 763
SfcI CTRYAG 1 cut(s) 561
Sse9I AATT 4 cut(s) 193, 422, 638, 680
SseBI AGGCCT 1 cut(s) 289
SsiI CCGC 1 cut(s) 713
SspMI CTAG 5 cut(s) 9, 75, 482, 588, 716
SstI GAGCTC 1 cut(s) 699
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 2 cut(s) 61, 814
TaaI ACNGT 1 cut(s) 653
TaiI ACGT 1 cut(s) 136
TaqI TCGA 2 cut(s) 184, 403
TasI AATT 4 cut(s) 193, 422, 638, 680
TfiI GAWTC 3 cut(s) 478, 491, 541
Tru1I TTAA 2 cut(s) 152, 635
Tru9I TTAA 2 cut(s) 152, 635
TscAI CASTG 3 cut(s) 533, 616, 658
TseFI GTSAC 2 cut(s) 202, 740
TseI GCWGC 2 cut(s) 447, 830
Tsp45I GTSAC 2 cut(s) 202, 740
TspDTI ATGAA 3 cut(s) 84, 333, 678
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 3 cut(s) 533, 616, 658
XagI CCTNNNNNAGG 1 cut(s) 439
XapI RAATTY 3 cut(s) 193, 422, 680
XbaI TCTAGA 1 cut(s) 481
XspI CTAG 5 cut(s) 9, 75, 482, 588, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.