RchiOBHm_Chr5g0035851

Phosphoenolpyruvate carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020496
Physical Location & Seq
Forward (+)
29866905 .. 29868061
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31469

Sequence Viewer

Length: 408 bp
ATGCTGTCAAACTGTCTAGTCACAAAAGATGTCTCACTTTTATCTAGGTGGATGACTATTGATCTCTATATTCGAGAAGTGGATAGCCTGAAATTTGAACTATCCATGAATAGGTGCAGCGATATCTTGTCAAAACTGGCACAAGAGATTCTAGAACACTCAGAATCTGCATCTGAGGATCACCAAAGTTGGGATCATCAGTGTTATAATAGGAATCAGGTAAAGCAACAAGCTGCAACACTTCCAACACAACTTCCAGCTAGAGCTAATCAACCCTCTTGCACTGGTGAACATCCGATTATCCTCTTAAAACTACTTTTTAAAGAAAAAATTTATAATAAAATTGAGGATCTCGAAAGTTGTGTTACTTTTATATTATTTTCATATGCTGCCATCCTTATTGCATAA

Protein Analysis

135

Amino Acids

15.5

Weight (kDa)

5.51

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPcase PF00311 7 - 78 5.5e-06 Phosphoenolpyruvate carboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 207, 336
AclWI GGATC 3 cut(s) 186, 201, 357
AcsI RAATTY 2 cut(s) 92, 330
AfiI CCNNNNNNNGG 2 cut(s) 111, 190
AgsI TTSAA 1 cut(s) 98
AluBI AGCT 3 cut(s) 233, 260, 266
AluI AGCT 3 cut(s) 233, 260, 266
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 3 cut(s) 186, 201, 357
AlwNI CAGNNNCTG 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 117, 233, 389
ApoI RAATTY 2 cut(s) 92, 330
AsuHPI GGTGA 2 cut(s) 173, 299
BbvI GCAGC 3 cut(s) 129, 220, 376
BccI CCATC 1 cut(s) 401
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 4 cut(s) 17, 45, 152, 261
BisI GCNGC 3 cut(s) 118, 234, 390
BlsI GCNGC 3 cut(s) 119, 235, 391
BmsI GCATC 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 190
Bse1I ACTGG 2 cut(s) 141, 289
BseGI GGATG 3 cut(s) 57, 292, 393
BseLI CCNNNNNNNGG 2 cut(s) 111, 190
BseMII CTCAG 2 cut(s) 165, 174
BseNI ACTGG 2 cut(s) 141, 289
BseXI GCAGC 3 cut(s) 129, 220, 376
BsgI GTGCAG 1 cut(s) 136
BslI CCNNNNNNNGG 2 cut(s) 111, 190
BsmAI GTCTC 1 cut(s) 37
Bsp143I GATC 4 cut(s) 61, 178, 193, 349
BspCNI CTCAG 2 cut(s) 166, 173
BspPI GGATC 3 cut(s) 186, 201, 357
BsrI ACTGG 2 cut(s) 141, 289
BssMI GATC 4 cut(s) 61, 178, 193, 349
Bst4CI ACNGT 1 cut(s) 14
BstDEI CTNAG 2 cut(s) 160, 174
BstF5I GGATG 3 cut(s) 57, 292, 393
BstKTI GATC 4 cut(s) 64, 181, 196, 352
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 4 cut(s) 61, 178, 193, 349
BstV1I GCAGC 3 cut(s) 129, 220, 376
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BtsCI GGATG 3 cut(s) 57, 292, 393
BtsIMutI CAGTG 2 cut(s) 206, 282
CaiI CAGNNNCTG 1 cut(s) 167
CviAII CATG 1 cut(s) 106
CviJI RGCY 4 cut(s) 87, 233, 260, 266
CviKI_1 RGCY 4 cut(s) 87, 233, 260, 266
DdeI CTNAG 2 cut(s) 160, 174
DpnI GATC 4 cut(s) 63, 180, 195, 351
DpnII GATC 4 cut(s) 61, 178, 193, 349
DraI TTTAAA 1 cut(s) 322
Eco32I GATATC 1 cut(s) 124
EcoRV GATATC 1 cut(s) 124
FaeI CATG 1 cut(s) 109
FaiI YATR 8 cut(s) 69, 107, 207, 336, 374, 385, 387, 406
FatI CATG 1 cut(s) 105
FauNDI CATATG 1 cut(s) 385
Fnu4HI GCNGC 3 cut(s) 118, 234, 390
FokI GGATG 3 cut(s) 64, 279, 380
Fsp4HI GCNGC 3 cut(s) 118, 234, 390
FspBI CTAG 4 cut(s) 17, 45, 152, 261
GluI GCNGC 3 cut(s) 118, 234, 390
Hin1II CATG 1 cut(s) 109
HinfI GANTC 3 cut(s) 148, 164, 214
HphI GGTGA 2 cut(s) 173, 299
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 3 cut(s) 163, 175, 297
Hpy188III TCNNGA 3 cut(s) 74, 152, 353
Hpy8I GTNNAC 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 5 cut(s) 117, 170, 236, 282, 404
HpyF3I CTNAG 2 cut(s) 160, 174
Hsp92II CATG 1 cut(s) 109
Kzo9I GATC 4 cut(s) 61, 178, 193, 349
LpnPI CCDG 5 cut(s) 101, 122, 203, 270, 270
Lsp1109I GCAGC 3 cut(s) 129, 220, 376
LweI GCATC 1 cut(s) 179
MaeI CTAG 4 cut(s) 17, 45, 152, 261
MaeIII GTNAC 2 cut(s) 19, 364
MalI GATC 4 cut(s) 63, 180, 195, 351
MboI GATC 4 cut(s) 61, 178, 193, 349
MflI RGATCY 1 cut(s) 349
MluCI AATT 3 cut(s) 92, 330, 342
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 4 cut(s) 169, 286, 314, 340
MseI TTAA 2 cut(s) 308, 321
NdeI CATATG 1 cut(s) 385
NdeII GATC 4 cut(s) 61, 178, 193, 349
NlaIII CATG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 148, 164, 214
PkrI GCNGC 3 cut(s) 119, 235, 391
PsiI TTATAA 2 cut(s) 207, 336
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 349
SaqAI TTAA 2 cut(s) 308, 321
SatI GCNGC 3 cut(s) 118, 234, 390
Sau3AI GATC 4 cut(s) 61, 178, 193, 349
SetI ASST 6 cut(s) 50, 116, 222, 235, 262, 268
SfaNI GCATC 1 cut(s) 179
Sse9I AATT 3 cut(s) 92, 330, 342
SspMI CTAG 4 cut(s) 17, 45, 152, 261
TaaI ACNGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 73, 354
TasI AATT 3 cut(s) 92, 330, 342
TfiI GAWTC 3 cut(s) 148, 164, 214
Tru1I TTAA 2 cut(s) 308, 321
Tru9I TTAA 2 cut(s) 308, 321
TscAI CASTG 2 cut(s) 206, 289
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 3 cut(s) 117, 233, 389
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 122, 372
TspRI CASTG 2 cut(s) 206, 289
XapI RAATTY 2 cut(s) 92, 330
XbaI TCTAGA 1 cut(s) 151
XspI CTAG 4 cut(s) 17, 45, 152, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.