RchiOBHm_Chr5g0064821

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018912
Physical Location & Seq
Forward (+)
70749862 .. 70752950
3089 bp
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UTR
Exon/CDS
Intron
PRQ34075

Sequence Viewer

Length: 330 bp
ATGGCTTTGAGCACCCACAGGGCCTCCTCATCTCGTGATTGGGCTGAACCACCTCCCCAGTGGAATTTCGATGTTTTCTTGAGTTTCAGGGGTGAAGACACCCGCACGGGTTTCGTCTCCCATTTATTCCGCGAACTGCAGTACCGGAAAGTATTTAAGACTTTCAAGGACGACCGAGAGCTTGAGATCGGAAGGGTTTTTAGAGCTTTCCAAGTGTTTTGTAGATTATGCCAAAGGGCTTCCATTAGCTCTAAAAACTCTGGGAAGTTCTTTACCGGAGGTGCAAAAGGCTTGAACAATTACCGGATCTCCCATCGAATGAGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

109

Amino Acids

12.82

Weight (kDa)

10.63

Isoelectric Point (pI)

16.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 66 1.5e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018912)

Species Orthologous Gene IDs
prunus_persica Prupe.2G113300_v2.0.a1 Prupe.8G053200_v2.0.a1 Prupe.8G053200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0064821
rosa_laevigata RLG00000013472
rosa_rugosa Rorug05G0339300 Rorug05G0366500
rosa_samantha Rh2AG342000 Rh2AG342100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 132
AciI CCGC 2 cut(s) 103, 130
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 1 cut(s) 64
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 2 cut(s) 166, 295
AluBI AGCT 3 cut(s) 181, 206, 249
AluI AGCT 3 cut(s) 181, 206, 249
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 314
AoxI GGCC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 64
AspS9I GGNCC 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 104
BaeI ACNNNNGTAYC 2 cut(s) 125, 158
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 1 cut(s) 321
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BcoDI GTCTC 1 cut(s) 121
BfmI CTRYAG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 21
BmrI ACTGGG 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 100, 203
BsaWI WCCGGW 3 cut(s) 144, 275, 303
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 293, 323
Bse1I ACTGG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 3 cut(s) 145, 276, 304
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 2 cut(s) 186, 306
BspACI CCGC 2 cut(s) 103, 130
BspANI GGCC 1 cut(s) 23
BspFNI CGCG 1 cut(s) 132
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 1 cut(s) 314
BsrI ACTGG 1 cut(s) 58
BssMI GATC 2 cut(s) 186, 306
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 2 cut(s) 189, 309
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 186, 306
BstMCI CGRYCG 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 137
BstUI CGCG 1 cut(s) 132
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuRI GGCC 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 142
CviJI RGCY 8 cut(s) 5, 23, 44, 181, 206, 239, 249, 291
CviKI_1 RGCY 8 cut(s) 5, 23, 44, 181, 206, 239, 249, 291
CviQI GTAC 1 cut(s) 142
DpnI GATC 2 cut(s) 188, 308
DpnII GATC 2 cut(s) 186, 306
EcoO109I RGGNCCY 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 121
FaiI YATR 1 cut(s) 229
FauI CCCGC 1 cut(s) 110
HaeIII GGCC 1 cut(s) 23
HapII CCGG 3 cut(s) 145, 276, 304
HpaII CCGG 3 cut(s) 145, 276, 304
HphI GGTGA 1 cut(s) 104
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 2 cut(s) 35, 79
HpyAV CCTTC 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 139, 284
Kzo9I GATC 2 cut(s) 186, 306
LpnPI CCDG 7 cut(s) 4, 71, 73, 158, 246, 289, 317
MalI GATC 2 cut(s) 188, 308
MboI GATC 2 cut(s) 186, 306
MboII GAAGA 1 cut(s) 107
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 2 cut(s) 64, 298
MnlI CCTC 5 cut(s) 34, 37, 63, 272, 315
MseI TTAA 2 cut(s) 156, 328
MspI CCGG 3 cut(s) 145, 276, 304
MvnI CGCG 1 cut(s) 132
NdeII GATC 2 cut(s) 186, 306
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 156, 328
Sau3AI GATC 2 cut(s) 186, 306
Sau96I GGNCC 1 cut(s) 21
SduI GDGCHC 1 cut(s) 14
SetI ASST 5 cut(s) 55, 183, 208, 251, 283
SfcI CTRYAG 1 cut(s) 137
SmlI CTYRAG 2 cut(s) 79, 182
SmoI CTYRAG 2 cut(s) 79, 182
Sse9I AATT 2 cut(s) 64, 298
SsiI CCGC 2 cut(s) 103, 130
TaqI TCGA 2 cut(s) 69, 316
TaqII GACCGA 1 cut(s) 189
TasI AATT 2 cut(s) 64, 298
Tru1I TTAA 2 cut(s) 156, 328
Tru9I TTAA 2 cut(s) 156, 328
TscAI CASTG 1 cut(s) 65
TspRI CASTG 1 cut(s) 65
XapI RAATTY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.