RLG00000013472

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018912
Physical Location & Seq
Forward (+)
32061586 .. 32062744
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013472

Sequence Viewer

Length: 483 bp
ATGCTCGAGGAAGATGGGGTTGCCGCAATCTCTGGAGTGTCTGCAACTGGCAGAGCCTCAGATGGGCTTGACTTCCGAGCGGCGAAGTCCAAGCAAACCGGTTCCGGCTTGCTGTTCAACCTGGCTCAACTGTGGGAGACACTGACGTCTACTCCACTTCCGGCGATTGTTTCAAAGTCGTCGTTGAATTCAAACCGGAAATGGAAACTGTTCGAGTGTGGCCAGAACTGGATTGGATTTTGGAGGAGCCGTAATCCGAAGGTGGAGATCGGGGAGATAGAGGAGAGAGAAACCTTAGATACTCGCATGAGTTTCATATCCCATTTATACCACGAGTTGCAATGCGTGCAAAGGATTAAAGCATTCAAGGATGATCAATCACTTGAAAGAGGGAGAACTATTTCAGAGCTCTTCAGGGCAATTGAAGAATCAAGGTTTGTGATTGTTTCTAGCATAACAATGCTTCTTCGATATGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

161

Amino Acids

18.07

Weight (kDa)

7.79

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 98 - 149 1.3e-09 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018912)

Species Orthologous Gene IDs
prunus_persica Prupe.2G113300_v2.0.a1 Prupe.8G053200_v2.0.a1 Prupe.8G053200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0064821
rosa_laevigata RLG00000013472
rosa_rugosa Rorug05G0339300 Rorug05G0366500
rosa_samantha Rh2AG342000 Rh2AG342100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 145
AatII GACGTC 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 149
AciI CCGC 2 cut(s) 24, 80
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 397
AcyI GRCGYC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 63
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 7 cut(s) 118, 174, 187, 192, 367, 386, 425
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 1 cut(s) 409
AluI AGCT 1 cut(s) 409
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 5
AoxI GGCC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 187
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 2 cut(s) 209, 400
AvaI CYCGRG 1 cut(s) 5
BalI TGGCCA 1 cut(s) 222
BanII GRGCYC 1 cut(s) 411
BauI CACGAG 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 411
BccI CCATC 2 cut(s) 8, 56
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 122
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 450
BisI GCNGC 2 cut(s) 24, 81
BlsI GCNGC 2 cut(s) 25, 82
Bme1390I CCNGG 1 cut(s) 122
BmeT110I CYCGRG 1 cut(s) 5
BmiI GGNNCC 2 cut(s) 103, 248
BmrFI CCNGG 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 98, 195
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 52, 233
Bse3DI GCAATG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMI GCAATG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 52, 233
BseRI GAGGAG 2 cut(s) 259, 296
BshFI GGCC 1 cut(s) 222
BshTI ACCGGT 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 411
BsiHKCI CYCGRG 1 cut(s) 5
BsiSI CCGG 4 cut(s) 99, 105, 161, 196
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 2 cut(s) 267, 373
BspACI CCGC 2 cut(s) 24, 80
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 103, 248
BspQI GCTCTTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 2 cut(s) 52, 233
BssAI RCCGGY 1 cut(s) 98
BssMI GATC 2 cut(s) 267, 373
BssNI GRCGYC 1 cut(s) 146
BssSI CACGAG 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 132, 210
Bst6I CTCTTC 1 cut(s) 416
BstACI GRCGYC 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 110, 347
BstDEI CTNAG 2 cut(s) 58, 295
BstF5I GGATG 1 cut(s) 376
BstKTI GATC 2 cut(s) 270, 376
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 267, 373
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 2 cut(s) 110, 347
Cfr10I RCCGGY 1 cut(s) 98
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 1 cut(s) 307
CviJI RGCY 7 cut(s) 56, 67, 108, 125, 222, 249, 409
CviKI_1 RGCY 7 cut(s) 56, 67, 108, 125, 222, 249, 409
DdeI CTNAG 2 cut(s) 58, 295
DpnI GATC 2 cut(s) 269, 375
DpnII GATC 2 cut(s) 267, 373
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 397
Eco88I CYCGRG 1 cut(s) 5
EcoICRI GAGCTC 1 cut(s) 409
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 411
FaeI CATG 1 cut(s) 310
FaiI YATR 5 cut(s) 308, 317, 328, 455, 474
FatI CATG 1 cut(s) 306
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 24, 81
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 24, 81
FspBI CTAG 1 cut(s) 450
GluI GCNGC 2 cut(s) 24, 81
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 222
HapII CCGG 4 cut(s) 99, 105, 161, 196
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 1 cut(s) 310
HinfI GANTC 1 cut(s) 428
HpaII CCGG 4 cut(s) 99, 105, 161, 196
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 4 cut(s) 61, 77, 258, 406
Hpy188III TCNNGA 1 cut(s) 33
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 132, 210
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 44, 340, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 2 cut(s) 58, 295
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 2 cut(s) 267, 373
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 1 cut(s) 246
MaeI CTAG 1 cut(s) 450
MaeII ACGT 1 cut(s) 146
MalI GATC 2 cut(s) 269, 375
MbiI CCGCTC 1 cut(s) 80
MboI GATC 2 cut(s) 267, 373
MboII GAAGA 4 cut(s) 23, 403, 437, 458
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 2 cut(s) 187, 420
MluNI TGGCCA 1 cut(s) 222
MnlI CCTC 4 cut(s) 67, 237, 274, 383
Mox20I TGGCCA 1 cut(s) 222
MroXI GAANNNNTTC 2 cut(s) 209, 400
MscI TGGCCA 1 cut(s) 222
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 4 cut(s) 99, 105, 161, 196
MspR9I CCNGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 346
NdeII GATC 2 cut(s) 267, 373
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 103, 248
PaeR7I CTCGAG 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 416
PctI GAATGC 1 cut(s) 362
PdmI GAANNNNTTC 2 cut(s) 209, 400
PfeI GAWTC 1 cut(s) 428
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 2 cut(s) 25, 82
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 103, 248
PspXI VCTCGAGB 1 cut(s) 5
RseI CAYNNNNRTG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 416
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 2 cut(s) 24, 81
Sau3AI GATC 2 cut(s) 267, 373
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 411
SetI ASST 6 cut(s) 123, 149, 264, 296, 411, 437
Sfr274I CTCGAG 1 cut(s) 5
SlaI CTCGAG 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 2 cut(s) 187, 420
SsiI CCGC 2 cut(s) 24, 80
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 132, 210
TaiI ACGT 1 cut(s) 149
TaqI TCGA 3 cut(s) 6, 213, 469
TasI AATT 2 cut(s) 187, 420
TauI GCSGC 2 cut(s) 26, 83
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 147
TspDTI ATGAA 1 cut(s) 304
TspRI CASTG 1 cut(s) 147
XapI RAATTY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XhoI CTCGAG 1 cut(s) 5
XmiI GTMKAC 1 cut(s) 149
XmnI GAANNNNTTC 2 cut(s) 209, 400
XspI CTAG 1 cut(s) 450
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.