Rorug05G0366500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003424
Physical Location & Seq
Forward (+)
48832422 .. 48841266
8845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0366500.1

Sequence Viewer

Length: 948 bp
ATGGCGTCGTCTTCAGCTCTCTCAAGACTGTCCAGTATCAGCAGACACTTCAATCCCTCCCACGCAAACAAGGACGGTGTGGCCTTAATCAAGATTTCTCCAGAGGTTTCCGAAGCTTTGTCAAATGGGCGTGCCGTCGTTGCTCTTGAATCCACCATTATTTCCCATGGGATGCCGTATCCCAAGAATTTGGAGACTGCAAAGGAGGTTGAGGCAATTGTCAGGGAAAATGGGGCTGTTCCTGCCACCATTGCTATCTTGGATGGCACACCTTGCATAGGTCTAACTATAGAAGAACTGGAGAGGCTTGCCAGATTGGGTCCAAGAGCTCAGAAGACAGCTCGAAGAGACATTGCACATGTTGTGGCAACCAGAGGGAACGGTGCAACTACTGTTTCTGCAACCATGATTTTTGCTCATATGGTTGGCATCCCATTGTTTGTAACAGGAGGAATTGGAGGAGTACATCAACATGGCGAGCATACAATGGACATATCTTCTGATCTTACTGAGCTTGGAAGGACACCTGTAGCTGTCATCTCTGCTGGTGTAAAATCAATTTTGGATATCCCAAGGACCCTCGAATATTTGGAAACACAGGGAGTTTGTGTAGCTGCTTACAAGACCAATGAATTTCCAGCATTTTTCACAGAAGCAAGTGGCTGCAAGGTGCCTTGCCGTGTAGATACCCCAGATGATTGTGCTCGGCTTATAGATGCAAACCTTAAACTTGAGCTTGGAACTGGAATGTTGATTGCTGTTCCAATTCCTAAAGAACACTCGGCTTCTGGAAATTTAATTGAGTCTGCAATACAGACAGCTCTTAGAGAAGCTAGGGATAAGAACATAACAGGCAATGCTGCTACTCCATTCTTGCTTGCAAGAGTAAATGAACTAACTGGAGGAGCATCTTTGGCTTCGATTAGACATTGCCCTGGTGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

315

Amino Acids

33.18

Weight (kDa)

6.08

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 35 - 307 3.6e-124 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AcsI RAATTY 3 cut(s) 187, 632, 793
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 2 cut(s) 52, 149
AjnI CCWGG 1 cut(s) 934
Alw21I GWGCWC 2 cut(s) 331, 706
Alw26I GTCTC 2 cut(s) 188, 342
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 3 cut(s) 614, 663, 860
ApoI RAATTY 3 cut(s) 187, 632, 793
AspS9I GGNCC 2 cut(s) 320, 576
AvaII GGWCC 2 cut(s) 320, 576
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 3, 341
Bbv12I GWGCWC 2 cut(s) 331, 706
BbvI GCAGC 3 cut(s) 601, 650, 847
BccI CCATC 1 cut(s) 257
BceAI ACGGC 3 cut(s) 119, 160, 663
BciT130I CCWGG 1 cut(s) 936
BciVI GTATCC 1 cut(s) 189
BcoDI GTCTC 2 cut(s) 188, 342
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 2 cut(s) 288, 528
BfuI GTATCC 1 cut(s) 189
BisI GCNGC 3 cut(s) 615, 664, 861
BlsI GCNGC 3 cut(s) 616, 665, 862
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 2 cut(s) 320, 576
BmgT120I GGNCC 2 cut(s) 320, 576
BmiI GGNNCC 3 cut(s) 321, 578, 672
BmrFI CCNGG 1 cut(s) 936
BmsI GCATC 4 cut(s) 162, 438, 706, 917
BpiI GAAGAC 2 cut(s) 3, 341
BpmI CTGGAG 3 cut(s) 84, 320, 921
BpuEI CTTGAG 2 cut(s) 7, 752
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 166, 572, 934
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 4 cut(s) 33, 303, 748, 904
Bse3DI GCAATG 4 cut(s) 249, 351, 862, 928
BseBI CCWGG 1 cut(s) 936
BseDI CCNNGG 3 cut(s) 166, 572, 934
BseGI GGATG 3 cut(s) 177, 268, 429
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMI GCAATG 4 cut(s) 249, 351, 862, 928
BseMII CTCAG 2 cut(s) 344, 501
BseNI ACTGG 4 cut(s) 33, 303, 748, 904
BseRI GAGGAG 2 cut(s) 474, 918
BseXI GCAGC 3 cut(s) 601, 650, 847
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 670
BsiHKAI GWGCWC 2 cut(s) 331, 706
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 188, 342
BsnI GGCC 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 331, 706
Bsp143I GATC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 166
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 2 cut(s) 343, 502
BspLI GGNNCC 3 cut(s) 321, 578, 672
BspT107I GGYRCC 1 cut(s) 670
BsrDI GCAATG 4 cut(s) 249, 351, 862, 928
BsrI ACTGG 4 cut(s) 33, 303, 748, 904
BssECI CCNNGG 3 cut(s) 166, 572, 934
BssMI GATC 1 cut(s) 502
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 166, 572
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 4 cut(s) 30, 77, 383, 394
Bst6I CTCTTC 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 132, 309, 479, 879
BstDEI CTNAG 3 cut(s) 330, 510, 824
BstDSI CCRYGG 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 177, 268, 429
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 2 cut(s) 188, 342
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 5 cut(s) 140, 242, 251, 273, 914
BstNI CCWGG 1 cut(s) 936
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 934
BstSFI CTRYAG 2 cut(s) 288, 528
BstV1I GCAGC 3 cut(s) 601, 650, 847
BstV2I GAAGAC 2 cut(s) 3, 341
BstXI CCANNNNNNTGG 1 cut(s) 190
BsuI GTATCC 1 cut(s) 189
BsuRI GGCC 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 3 cut(s) 177, 268, 429
Cac8I GCNNGC 4 cut(s) 132, 309, 479, 879
Cfr13I GGNCC 2 cut(s) 320, 576
Csp6I GTAC 1 cut(s) 464
CviAII CATG 4 cut(s) 167, 359, 406, 473
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 3 cut(s) 330, 510, 824
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 166, 572
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 320, 576
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 576
EcoRII CCWGG 1 cut(s) 934
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 166, 572
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 2 cut(s) 166, 572
FaeI CATG 4 cut(s) 170, 362, 409, 476
FatI CATG 4 cut(s) 166, 358, 405, 472
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 3 cut(s) 615, 664, 861
FokI GGATG 3 cut(s) 184, 275, 416
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 3 cut(s) 615, 664, 861
FspBI CTAG 1 cut(s) 834
GluI GCNGC 3 cut(s) 615, 664, 861
GsuI CTGGAG 3 cut(s) 84, 320, 921
HaeIII GGCC 1 cut(s) 83
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 170, 362, 409, 476
HindIII AAGCTT 1 cut(s) 114
HinfI GANTC 2 cut(s) 149, 803
Hpy188I TCNGA 3 cut(s) 112, 333, 502
Hpy188III TCNNGA 5 cut(s) 24, 91, 101, 146, 789
Hpy99I CGWCG 2 cut(s) 10, 140
HpyAV CCTTC 1 cut(s) 513
HpyCH4III ACNGT 4 cut(s) 30, 77, 383, 394
HpyCH4V TGCA 9 cut(s) 200, 276, 356, 386, 401, 666, 719, 809, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 242, 251, 273, 914
HpyF3I CTNAG 3 cut(s) 330, 510, 824
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 170, 362, 409, 476
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 1 cut(s) 905
Lsp1109I GCAGC 3 cut(s) 601, 650, 847
LweI GCATC 4 cut(s) 162, 438, 706, 917
MaeI CTAG 1 cut(s) 834
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 5 cut(s) 3, 305, 346, 357, 489
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 331, 706
MluCI AATT 8 cut(s) 187, 216, 453, 558, 632, 765, 793, 798
MlyI GAGTC 1 cut(s) 812
MseI TTAA 3 cut(s) 86, 726, 797
MslI CAYNNNNRTG 1 cut(s) 471
MspR9I CCNGG 1 cut(s) 936
MunI CAATTG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 936
MwoI GCNNNNNNNGC 5 cut(s) 140, 242, 251, 273, 914
NcoI CCATGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 420
NdeII GATC 1 cut(s) 502
NlaIII CATG 4 cut(s) 170, 362, 409, 476
NlaIV GGNNCC 3 cut(s) 321, 578, 672
NmeAIII GCCGAG 2 cut(s) 685, 761
NspI RCATGY 1 cut(s) 362
PciI ACATGT 1 cut(s) 358
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 3 cut(s) 616, 665, 862
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 576
PscI ACATGT 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 934
PspGI CCWGG 1 cut(s) 934
PspN4I GGNNCC 3 cut(s) 321, 578, 672
PspPI GGNCC 2 cut(s) 320, 576
PspPPI RGGWCCY 1 cut(s) 576
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 86, 726, 797
SatI GCNGC 3 cut(s) 615, 664, 861
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 2 cut(s) 320, 576
SchI GAGTC 1 cut(s) 812
ScrFI CCNGG 1 cut(s) 936
SduI GDGCHC 2 cut(s) 331, 706
SfaNI GCATC 4 cut(s) 162, 438, 706, 917
SfcI CTRYAG 2 cut(s) 288, 528
SinI GGWCC 2 cut(s) 320, 576
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 22, 731
SmoI CTYRAG 2 cut(s) 22, 731
Sse9I AATT 8 cut(s) 187, 216, 453, 558, 632, 765, 793, 798
SspI AATATT 1 cut(s) 587
SspMI CTAG 1 cut(s) 834
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 934
StyI CCWWGG 2 cut(s) 166, 572
TaaI ACNGT 4 cut(s) 30, 77, 383, 394
TaqI TCGA 3 cut(s) 343, 582, 920
TasI AATT 8 cut(s) 187, 216, 453, 558, 632, 765, 793, 798
TatI WGTACW 1 cut(s) 463
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 3 cut(s) 86, 726, 797
Tru9I TTAA 3 cut(s) 86, 726, 797
TseI GCWGC 3 cut(s) 614, 663, 860
TspDTI ATGAA 2 cut(s) 645, 906
VpaK11BI GGWCC 2 cut(s) 320, 576
XagI CCTNNNNNAGG 1 cut(s) 276
XapI RAATTY 3 cut(s) 187, 632, 793
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.