AT2G28910

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003424
Physical Location & Seq
Reverse (-)
12415668 .. 12417615
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28910.2

Sequence Viewer

Length: 999 bp
ATGCCGGCGACAGCAGGAAGAGTGCGTATGCCCGCAAACAATCGGGTGCATAGTAGTGCAGCGCTTCAGACTCATGGAATATGGCAGAGTGCGATTGGTTATGATCCTTATGCACCAACTAGTAAAGAAGAGCCTAAGACGACACAACAGAAAACCGAAGATCCCGAGAACTCGTACGCGAGTTTTCAGGGACTTTTGGCTCTTGCTAGGATTACGGGTTCGAATAACGATGAGGCTCGTGGTTCTTGTAAGAAGTGTGGCCGTGTTGGGCATTTGACGTTTCAGTGTAGGAATTTCTTGAGTACTAAGGAGGATAAGGAGAAAGATCCTGGTGCGATTGAAGCTGCGGTTTTGTCGGGGTTGGAGAAGATTAGGAGAGGTGTTGGTAAAGGTGAGGTTGAGGAGGTGAGTAGTGAAGAGGAAGAGGAGAGTGAGAGTTCTGATTCTGATGTTGATTCTGAGATGGAGAGGATTATTGCTGAGAGGTTTGGGAAGAAGAAAGGTGGGAGTAGTGTTAAGAAGACGAGTTCGGTTAGGAAGAAGAAGAAGCGTGTTTCTGATGAGTCTGATTCTGATTCGGATTCTGGAGATAGGAAGAGAAGGCGGAGGTCGATGAAGAAGCGGAGTTCGCATAAGAGAAGGAGTTTGAGTGAGTCTGAGGATGAGGAAGAGGGAAGGAGTAAGAGAAGAAAGGAGAGGAGAGGAAGAAAGAGAGATGAAGATGATTCTGATGAGTCTGAGGATGAAGATGACAGACGTGTGAAGAGAAAGAGTAGAAAGGAGAAGAGGAGAAGGAGAAGCCGTCGTAATCATTCTGATGATTCTGATTCAGAGTCTTCTGAAGATGATAGAAGACAGAAGCGGAGGAACAAAGTCGCAGCTTCTTCTGACTCAGAAGCAAATGTTTCAGGCGATGATGTTTCACGCGTTGGACGTGGTTCTTCTAAGCGGTCTGAGAAGAAGAGCAGGAAACGTCATCACAGGAAAGAGCGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCHC_3

Protein Analysis

332

Amino Acids

37.83

Weight (kDa)

9.74

Isoelectric Point (pI)

97.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_3 PF13917 79 - 98 7.5e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 179, 927
AciI CCGC 6 cut(s) 33, 347, 604, 622, 862, 949
AclWI GGATC 3 cut(s) 98, 155, 320
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 2 cut(s) 50, 861
AfaI GTAC 2 cut(s) 176, 304
AfeI AGCGCT 1 cut(s) 63
AflIII ACRYGT 2 cut(s) 757, 925
AgsI TTSAA 1 cut(s) 341
AhlI ACTAGT 1 cut(s) 119
AjiI CACGTC 2 cut(s) 758, 935
AjnI CCWGG 1 cut(s) 328
AloI GAACNNNNNNTCC 2 cut(s) 202, 234
AluBI AGCT 2 cut(s) 344, 881
AluI AGCT 2 cut(s) 344, 881
AlwI GGATC 3 cut(s) 98, 155, 320
Ama87I CYCGRG 1 cut(s) 164
Aor51HI AGCGCT 1 cut(s) 63
AoxI GGCC 1 cut(s) 259
ApeKI GCWGC 3 cut(s) 59, 344, 878
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 1 cut(s) 64
AsuHPI GGTGA 2 cut(s) 404, 418
AsuII TTCGAA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 164
BauI CACGAG 1 cut(s) 237
BbsI GAAGAC 3 cut(s) 527, 828, 859
BbvI GCAGC 3 cut(s) 71, 331, 890
BccI CCATC 1 cut(s) 457
BceAI ACGGC 2 cut(s) 246, 786
BciT130I CCWGG 1 cut(s) 330
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 2 cut(s) 120, 207
BfoI RGCGCY 1 cut(s) 65
BisI GCNGC 3 cut(s) 60, 345, 879
BlsI GCNGC 3 cut(s) 61, 346, 880
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 330
BmeT110I CYCGRG 1 cut(s) 164
BmgBI CACGTC 2 cut(s) 758, 935
BmrFI CCNGG 1 cut(s) 330
BpiI GAAGAC 3 cut(s) 527, 828, 859
BpmI CTGGAG 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 319
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 787, 817
Bse118I RCCGGY 1 cut(s) 4
BseBI CCWGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 667, 748
BseMII CTCAG 6 cut(s) 450, 471, 648, 729, 906, 945
BseRI GAGGAG 4 cut(s) 416, 440, 712, 802
BseXI GCAGC 3 cut(s) 71, 331, 890
BsgI GTGCAG 1 cut(s) 78
Bsh1236I CGCG 2 cut(s) 179, 927
BshFI GGCC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 1 cut(s) 5
BsiWI CGTACG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 204
BsnI GGCC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 221
Bsp143I GATC 3 cut(s) 103, 160, 325
BspACI CCGC 6 cut(s) 33, 347, 604, 622, 862, 949
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 6 cut(s) 451, 472, 649, 730, 905, 946
BspFNI CGCG 2 cut(s) 179, 927
BspPI GGATC 3 cut(s) 98, 155, 320
BspQI GCTCTTC 2 cut(s) 123, 956
BspT104I TTCGAA 1 cut(s) 221
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 3 cut(s) 103, 160, 325
BssSI CACGAG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 330
Bst6I CTCTTC 9 cut(s) 13, 123, 411, 417, 590, 663, 758, 779, 956
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 6, 33
BstDEI CTNAG 9 cut(s) 135, 306, 459, 480, 657, 738, 892, 945, 954
BstF5I GGATG 2 cut(s) 667, 748
BstFNI CGCG 2 cut(s) 179, 927
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 3 cut(s) 106, 163, 328
BstMBI GATC 3 cut(s) 103, 160, 325
BstMWI GCNNNNNNNGC 2 cut(s) 341, 628
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BstUI CGCG 2 cut(s) 179, 927
BstV1I GCAGC 3 cut(s) 71, 331, 890
BstV2I GAAGAC 3 cut(s) 527, 828, 859
BstX2I RGATCY 2 cut(s) 160, 325
BstYI RGATCY 2 cut(s) 160, 325
BsuRI GGCC 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 927
BtrI CACGTC 2 cut(s) 758, 935
BtsCI GGATG 2 cut(s) 667, 748
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 6, 33
CfoI GCGC 1 cut(s) 64
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 2 cut(s) 175, 303
CviAII CATG 1 cut(s) 74
CviJI RGCY 7 cut(s) 133, 200, 236, 261, 344, 801, 881
CviKI_1 RGCY 7 cut(s) 133, 200, 236, 261, 344, 801, 881
CviQI GTAC 2 cut(s) 175, 303
DdeI CTNAG 9 cut(s) 135, 306, 459, 480, 657, 738, 892, 945, 954
DpnI GATC 3 cut(s) 105, 162, 327
DpnII GATC 3 cut(s) 103, 160, 325
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 9 cut(s) 13, 123, 411, 417, 590, 663, 758, 779, 956
EarI CTCTTC 9 cut(s) 13, 123, 411, 417, 590, 663, 758, 779, 956
EciI GGCGGA 1 cut(s) 619
Eco47III AGCGCT 1 cut(s) 63
Eco57I CTGAAG 2 cut(s) 50, 861
Eco88I CYCGRG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 1 cut(s) 77
FaiI YATR 7 cut(s) 29, 51, 75, 82, 102, 111, 633
FaqI GGGAC 1 cut(s) 204
FatI CATG 1 cut(s) 73
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 60, 345, 879
FokI GGATG 2 cut(s) 674, 755
Fsp4HI GCNGC 3 cut(s) 60, 345, 879
FspBI CTAG 2 cut(s) 120, 207
GlaI GCGC 1 cut(s) 63
GluI GCNGC 3 cut(s) 60, 345, 879
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 261
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 64
Hin1II CATG 1 cut(s) 77
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HpaII CCGG 1 cut(s) 5
HphI GGTGA 2 cut(s) 404, 418
Hpy188III TCNNGA 3 cut(s) 164, 298, 585
Hpy99I CGWCG 1 cut(s) 807
HpyAV CCTTC 4 cut(s) 594, 633, 669, 786
HpyCH4IV ACGT 4 cut(s) 278, 757, 934, 973
HpyCH4V TGCA 3 cut(s) 49, 59, 113
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 628
HpyF3I CTNAG 9 cut(s) 135, 306, 459, 480, 657, 738, 892, 945, 954
HpySE526I ACGT 4 cut(s) 278, 757, 934, 973
Hsp92II CATG 1 cut(s) 77
HspAI GCGC 1 cut(s) 62
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 103, 160, 325
LguI GCTCTTC 2 cut(s) 123, 956
LpnPI CCDG 8 cut(s) 18, 173, 315, 342, 570, 894, 952, 967
Lsp1109I GCAGC 3 cut(s) 71, 331, 890
MaeI CTAG 2 cut(s) 120, 207
MaeII ACGT 4 cut(s) 278, 757, 934, 973
MalI GATC 3 cut(s) 105, 162, 327
MboI GATC 3 cut(s) 103, 160, 325
MflI RGATCY 2 cut(s) 160, 325
MluCI AATT 1 cut(s) 292
MluI ACGCGT 1 cut(s) 925
MlyI GAGTC 6 cut(s) 64, 572, 662, 743, 842, 884
MmeI TCCRAC 2 cut(s) 342, 910
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 2 cut(s) 54, 816
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 2 cut(s) 179, 927
MwoI GCNNNNNNNGC 2 cut(s) 341, 628
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 3 cut(s) 103, 160, 325
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 77
NspV TTCGAA 1 cut(s) 221
PciSI GCTCTTC 2 cut(s) 123, 956
PcsI WCGNNNNNNNCGW 1 cut(s) 931
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 8 cut(s) 443, 455, 569, 575, 581, 725, 821, 827
Pfl23II CGTACG 1 cut(s) 174
PkrI GCNGC 3 cut(s) 61, 346, 880
PleI GAGTC 6 cut(s) 64, 571, 661, 742, 841, 884
PpsI GAGTC 6 cut(s) 64, 571, 661, 742, 841, 884
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspLI CGTACG 1 cut(s) 174
PsuI RGATCY 2 cut(s) 160, 325
RsaI GTAC 2 cut(s) 176, 304
RsaNI GTAC 2 cut(s) 175, 303
RseI CAYNNNNRTG 2 cut(s) 54, 816
SapI GCTCTTC 2 cut(s) 123, 956
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 3 cut(s) 60, 345, 879
Sau3AI GATC 3 cut(s) 103, 160, 325
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 6 cut(s) 64, 572, 662, 743, 842, 884
ScrFI CCNGG 1 cut(s) 330
SfuI TTCGAA 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 54, 816
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
SpeI ACTAGT 1 cut(s) 119
Sse9I AATT 1 cut(s) 292
SsiI CCGC 6 cut(s) 33, 347, 604, 622, 862, 949
SspMI CTAG 2 cut(s) 120, 207
StyD4I CCNGG 1 cut(s) 328
TaiI ACGT 4 cut(s) 281, 760, 937, 976
TaqI TCGA 2 cut(s) 221, 611
TasI AATT 1 cut(s) 292
TatI WGTACW 1 cut(s) 302
TfiI GAWTC 8 cut(s) 443, 455, 569, 575, 581, 725, 821, 827
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 1 cut(s) 290
TseI GCWGC 3 cut(s) 59, 344, 878
TspDTI ATGAA 3 cut(s) 629, 732, 759
TspRI CASTG 1 cut(s) 290
XapI RAATTY 1 cut(s) 292
XspI CTAG 2 cut(s) 120, 207
ZrmI AGTACT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.