RchiOBHm_Chr5g0071351

gamma carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020551
Physical Location & Seq
Reverse (-)
77221109 .. 77224370
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34645

Sequence Viewer

Length: 243 bp
ATGACCATCAGAGGCGTCGATTTCAAATGGTGCGATGGATTCTTCTTGTCGATGCTCGCGACCGTGTCTAGGCATCGGACTCTCATGAATGTATTCGATAAAGCTCCTGTTGTTGACAAGGATGCATTTGTGGCCCCAAGTGCCTCCATCGTTGGTGATGTTCAAGTGGGAAGAGGATCTTCTATTTGGTATGGATGTGTATTGAGTGTTAGCAAGCCTGAATCAAGCAATTTTCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

80

Amino Acids

8.77

Weight (kDa)

7.83

Isoelectric Point (pI)

17.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020551)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0071351
rosa_laevigata RLG00000002964
rosa_multiflora Rmu_sc0006990.1_g000002
rosa_roxburghii Rroxscaffold_1G00056810
rosa_samantha Rh5AG468000 Rh5CG510900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 59
AclWI GGATC 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 2 cut(s) 25, 164
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
AlwI GGATC 1 cut(s) 184
AoxI GGCC 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 167
BccI CCATC 3 cut(s) 14, 29, 155
BfaI CTAG 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 3 cut(s) 42, 82, 112
BsaHI GRCGYC 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 69
BseGI GGATG 2 cut(s) 127, 200
BseLI CCNNNNNNNGG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 176
Bsp68I TCGCGA 1 cut(s) 59
BspANI GGCC 1 cut(s) 134
BspFNI CGCG 1 cut(s) 59
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 1 cut(s) 184
BssMI GATC 1 cut(s) 176
BssNI GRCGYC 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 57, 215
BstF5I GGATG 2 cut(s) 127, 200
BstFNI CGCG 1 cut(s) 59
BstKTI GATC 1 cut(s) 179
BstMBI GATC 1 cut(s) 176
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 131, 140
BstUI CGCG 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 127, 200
BtuMI TCGCGA 1 cut(s) 59
Cac8I GCNNGC 2 cut(s) 57, 215
CciI TCATGA 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 1 cut(s) 4
CviAII CATG 2 cut(s) 85, 236
CviJI RGCY 3 cut(s) 104, 134, 217
CviKI_1 RGCY 3 cut(s) 104, 134, 217
DpnI GATC 1 cut(s) 178
DpnII GATC 1 cut(s) 176
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 127
FaeI CATG 2 cut(s) 88, 239
FaiI YATR 3 cut(s) 86, 192, 237
FalI AAGNNNNNCTT 2 cut(s) 163, 195
FatI CATG 2 cut(s) 84, 235
FokI GGATG 2 cut(s) 134, 207
FspBI CTAG 1 cut(s) 69
HaeIII GGCC 1 cut(s) 134
HgaI GACGC 1 cut(s) 4
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 2 cut(s) 88, 239
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 39, 79, 221
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 3 cut(s) 11, 78, 242
Hpy188III TCNNGA 2 cut(s) 58, 85
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4V TGCA 1 cut(s) 125
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 140
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 2 cut(s) 88, 239
Kzo9I GATC 1 cut(s) 176
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 2 cut(s) 120, 231
LweI GCATC 3 cut(s) 42, 82, 112
MaeI CTAG 1 cut(s) 69
MalI GATC 1 cut(s) 178
MboI GATC 1 cut(s) 176
MboII GAAGA 3 cut(s) 34, 171, 183
MflI RGATCY 1 cut(s) 176
MluCI AATT 1 cut(s) 229
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 3 cut(s) 5, 154, 167
Mph1103I ATGCAT 1 cut(s) 127
MroXI GAANNNNTTC 1 cut(s) 92
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 131, 140
NdeII GATC 1 cut(s) 176
NlaIII CATG 2 cut(s) 88, 239
NlaIV GGNNCC 1 cut(s) 135
NruI TCGCGA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 127
PagI TCATGA 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 39, 221
PflFI GACNNNGTC 1 cut(s) 64
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 64
RruI TCGCGA 1 cut(s) 59
Sau3AI GATC 1 cut(s) 176
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 1 cut(s) 73
SetI ASST 1 cut(s) 106
SfaNI GCATC 3 cut(s) 42, 82, 112
Sse9I AATT 1 cut(s) 229
SspMI CTAG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 3 cut(s) 18, 50, 96
TasI AATT 1 cut(s) 229
TfiI GAWTC 2 cut(s) 39, 221
TspDTI ATGAA 2 cut(s) 101, 224
Tth111I GACNNNGTC 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.