Rh5AG468000

gamma carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0020551
Physical Location & Seq
Reverse (-)
80304192 .. 80306563
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG468000.1

Sequence Viewer

Length: 390 bp
ATGACCATCAGAGGCGTCGATTTCAAATGGTGCGATGGATTCTTCTTGTCGATGCTCGCGACCGTGTCTAGGCATCGGACTCTTATGAATGTATTCGATAAAGCTCCTGTTGTTGACAAGGATGCATTTGTGGCCCCAAGTGCCTCCGTCGTTGGTGATGTTCAAGTGGGAAGAGGATCTTCTATTTGGTATGGATGTGTATTGAGTGATATCTATCTGAGCTACTCGGGGAGCATCACAAGCTCGGTCCATTCCTCCCTGTGCTACCGAATTGCTGCAGGTTCCCTCTATTTTTGCAAATGGTCAACGAGAGATTGGAGAGTGCTTAGCAAGCAAGGGCATTGGAGAACGTTGAAAAGAAAGGGCATTTCGTGGGGAATTAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

129

Amino Acids

14.48

Weight (kDa)

9.73

Isoelectric Point (pI)

13.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020551)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0071351
rosa_laevigata RLG00000002964
rosa_multiflora Rmu_sc0006990.1_g000002
rosa_roxburghii Rroxscaffold_1G00056810
rosa_samantha Rh5AG468000 Rh5CG510900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AccII CGCG 1 cut(s) 59
AclI AACGTT 1 cut(s) 350
AclWI GGATC 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 3 cut(s) 25, 164, 355
AluBI AGCT 3 cut(s) 104, 222, 243
AluI AGCT 3 cut(s) 104, 222, 243
AlwI GGATC 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 133, 247
AsuHPI GGTGA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 14, 29
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 1 cut(s) 276
BlpI GCTNAGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 277
Bme18I GGWCC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 133, 247
BmiI GGNNCC 2 cut(s) 135, 283
BmsI GCATC 4 cut(s) 42, 82, 112, 243
Bpu1102I GCTNAGC 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 213
BseGI GGATG 2 cut(s) 127, 200
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 134
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 226
Bsp143I GATC 1 cut(s) 176
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp68I TCGCGA 1 cut(s) 59
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 135, 283
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 1 cut(s) 184
BssMI GATC 1 cut(s) 176
BssNI GRCGYC 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 166
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 57, 332
BstDEI CTNAG 2 cut(s) 218, 326
BstF5I GGATG 2 cut(s) 127, 200
BstFNI CGCG 1 cut(s) 59
BstKTI GATC 1 cut(s) 179
BstMBI GATC 1 cut(s) 176
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 4 cut(s) 131, 140, 240, 331
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 1 cut(s) 134
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 127, 200
BtuMI TCGCGA 1 cut(s) 59
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 57, 332
Cfr13I GGNCC 2 cut(s) 133, 247
CseI GACGC 1 cut(s) 4
CviJI RGCY 4 cut(s) 104, 134, 222, 243
CviKI_1 RGCY 4 cut(s) 104, 134, 222, 243
DdeI CTNAG 2 cut(s) 218, 326
DpnI GATC 1 cut(s) 178
DpnII GATC 1 cut(s) 176
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 226
EcoRV GATATC 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 127
FaiI YATR 3 cut(s) 86, 192, 388
FalI AAGNNNNNCTT 2 cut(s) 163, 195
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 2 cut(s) 134, 207
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 69
GluI GCNGC 1 cut(s) 276
HaeIII GGCC 1 cut(s) 134
HgaI GACGC 1 cut(s) 4
Hin1I GRCGYC 1 cut(s) 15
HincII GTYRAC 2 cut(s) 115, 306
HindII GTYRAC 2 cut(s) 115, 306
HinfI GANTC 2 cut(s) 39, 79
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 115, 306
Hpy188I TCNGA 3 cut(s) 11, 78, 219
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 2 cut(s) 115, 306
Hpy99I CGWCG 2 cut(s) 20, 152
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 3 cut(s) 125, 278, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 140, 240, 331
HpyF3I CTNAG 2 cut(s) 218, 326
HpySE526I ACGT 1 cut(s) 350
Hsp92I GRCGYC 1 cut(s) 15
Kzo9I GATC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 109, 231
LpnPI CCDG 3 cut(s) 120, 264, 272
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 4 cut(s) 42, 82, 112, 243
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 350
MalI GATC 1 cut(s) 178
MboI GATC 1 cut(s) 176
MboII GAAGA 3 cut(s) 34, 171, 183
MflI RGATCY 1 cut(s) 176
MluCI AATT 2 cut(s) 270, 378
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 5 cut(s) 5, 154, 167, 265, 296
Mph1103I ATGCAT 1 cut(s) 127
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 1 cut(s) 381
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 4 cut(s) 131, 140, 240, 331
NdeII GATC 1 cut(s) 176
NlaIV GGNNCC 2 cut(s) 135, 283
NruI TCGCGA 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp1406I AACGTT 1 cut(s) 350
PspN4I GGNNCC 2 cut(s) 135, 283
PspPI GGNCC 2 cut(s) 133, 247
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 64
RruI TCGCGA 1 cut(s) 59
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 1 cut(s) 176
Sau96I GGNCC 2 cut(s) 133, 247
SchI GAGTC 1 cut(s) 73
SetI ASST 5 cut(s) 106, 224, 245, 283, 353
SfaNI GCATC 4 cut(s) 42, 82, 112, 243
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 247
Sse9I AATT 2 cut(s) 270, 378
SspMI CTAG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 353
TaqI TCGA 3 cut(s) 18, 50, 96
TaqII GACCGA 1 cut(s) 235
TasI AATT 2 cut(s) 270, 378
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.