RchiOBHm_Chr6g0252831

Endo-1,4-beta-xylanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020585
Physical Location & Seq
Reverse (-)
7955392 .. 7956275
884 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22669

Sequence Viewer

Length: 666 bp
ATGGACGCATATTGGAGAGCACATAATATTGTGAAGAGATACAAGGGCAAACTCATTGCTTGGAATGTTGTTAACGAAAATTTGCATTTTGATTTCTTTGAAAGAAGGATTGGTGCAGCAGCTTCAGGCATTATGTATAATTGGGCTCTTAAAGCTGATGGAGCAACCCCTTTATTCTTGAATGATTTCAATACCGTTGAGGATAGCAGAGATTGGAAAGCCTCGCCAAAAGCGTACATTAACAGGCTCAGAGGGATTAAAAGGGTAAAGGATAATTTCAGAGGCCGATTCGGGATTGGACTTGAGTCACATTTTGCATGGTACTTGGAGCAGATTCTAAGGGAGGTACATTCTCACCCACAAATTCAAGGAATTGTGATTTGGTCTGCATGGCAACCTCAAGGATGTTACAGAATGTGCTTAACCGATTACAATTTCAAAAACTTGGCCACTGGTAATGTTGTGGACAAGTTACTACATGAATGGGGTTTGTGGGCTTTCGATTCGACTTCTGCCACAACAGACGCTAATGGTTTCTTTGAGGCCTCCCTCTTCCACGGGGAATCGGGGATTATGAAGTTAACATTACTCACCCCTGTCACAAATTCCTCCTTGGTTCACAGATTCAATGTGGCACCAAAATCTGAGTCCCAGGAACCATTGTAG

Protein Analysis

221

Amino Acids

25.39

Weight (kDa)

8.83

Isoelectric Point (pI)

22.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_10 PF00331 8 - 106 3.6e-08 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020585)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0252831
rosa_laevigata RLG00000015062
rosa_rugosa Rorug05G0538900 Rorug05G0539000
rosa_samantha Rh6AG055000 Rh6BG049100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 634
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 3 cut(s) 79, 363, 604
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 3 cut(s) 236, 323, 348
AgsI TTSAA 6 cut(s) 101, 181, 190, 368, 439, 628
AjnI CCWGG 1 cut(s) 651
AjuI GAANNNNNNNTTGG 4 cut(s) 93, 125, 364, 396
AluBI AGCT 2 cut(s) 122, 155
AluI AGCT 2 cut(s) 122, 155
Alw21I GWGCWC 1 cut(s) 22
AoxI GGCC 3 cut(s) 283, 447, 543
ApeKI GCWGC 2 cut(s) 116, 119
ApoI RAATTY 3 cut(s) 79, 363, 604
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
Asp700I GAANNNNTTC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 347, 583
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 1 cut(s) 634
BanII GRGCYC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 2 cut(s) 128, 131
BccI CCATC 1 cut(s) 152
BciT130I CCWGG 1 cut(s) 653
BisI GCNGC 2 cut(s) 117, 120
BlsI GCNGC 2 cut(s) 118, 121
Bme1390I CCNGG 1 cut(s) 653
BmiI GGNNCC 2 cut(s) 636, 657
BmrFI CCNGG 1 cut(s) 653
BoxI GACNNNNGTC 1 cut(s) 304
BpuEI CTTGAG 2 cut(s) 323, 384
BsaJI CCNNGG 3 cut(s) 556, 612, 651
Bse1I ACTGG 1 cut(s) 457
Bse3DI GCAATG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 3 cut(s) 556, 612, 651
BseGI GGATG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 262, 636
BseNI ACTGG 1 cut(s) 457
BseXI GCAGC 2 cut(s) 128, 131
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 3 cut(s) 285, 449, 545
BshNI GGYRCC 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 22
BslFI GGGAC 1 cut(s) 634
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 3 cut(s) 285, 449, 545
Bsp1286I GDGCHC 2 cut(s) 22, 148
BspANI GGCC 3 cut(s) 285, 449, 545
BspCNI CTCAG 2 cut(s) 261, 637
BspLI GGNNCC 2 cut(s) 636, 657
BspT107I GGYRCC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 54
BsrI ACTGG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 556, 612, 651
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 2 cut(s) 29, 557
BstDEI CTNAG 3 cut(s) 248, 338, 645
BstDSI CCRYGG 1 cut(s) 556
BstF5I GGATG 1 cut(s) 410
BstMWI GCNNNNNNNGC 2 cut(s) 152, 161
BstNI CCWGG 1 cut(s) 653
BstPAI GACNNNNGTC 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 651
BstV1I GCAGC 2 cut(s) 128, 131
BsuRI GGCC 3 cut(s) 285, 449, 545
BtgI CCRYGG 1 cut(s) 556
BtsCI GGATG 1 cut(s) 410
BtsIMutI CAGTG 1 cut(s) 450
CseI GACGC 2 cut(s) 14, 533
Csp6I GTAC 3 cut(s) 235, 322, 347
CviAII CATG 3 cut(s) 318, 390, 479
CviJI RGCY 9 cut(s) 122, 146, 155, 221, 247, 285, 449, 497, 545
CviKI_1 RGCY 9 cut(s) 122, 146, 155, 221, 247, 285, 449, 497, 545
CviQI GTAC 3 cut(s) 235, 322, 347
DdeI CTNAG 3 cut(s) 248, 338, 645
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 29, 557
EarI CTCTTC 2 cut(s) 29, 557
Eco130I CCWWGG 1 cut(s) 612
Eco147I AGGCCT 1 cut(s) 545
Eco24I GRGCYC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 3 cut(s) 321, 393, 482
FaiI YATR 8 cut(s) 10, 24, 134, 138, 319, 391, 480, 575
FaqI GGGAC 1 cut(s) 634
FatI CATG 3 cut(s) 317, 389, 478
Fnu4HI GCNGC 2 cut(s) 117, 120
FokI GGATG 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 2 cut(s) 117, 120
GluI GCNGC 2 cut(s) 117, 120
HaeIII GGCC 3 cut(s) 285, 449, 545
HgaI GACGC 2 cut(s) 14, 533
Hin1II CATG 3 cut(s) 321, 393, 482
HincII GTYRAC 2 cut(s) 73, 582
HindII GTYRAC 2 cut(s) 73, 582
HinfI GANTC 7 cut(s) 288, 305, 334, 503, 563, 624, 647
HpaI GTTAAC 2 cut(s) 73, 582
HphI GGTGA 2 cut(s) 347, 583
Hpy166II GTNNAC 4 cut(s) 73, 466, 582, 619
Hpy188I TCNGA 3 cut(s) 251, 281, 646
Hpy188III TCNNGA 2 cut(s) 178, 292
Hpy8I GTNNAC 4 cut(s) 73, 466, 582, 619
HpyAV CCTTC 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 4 cut(s) 85, 116, 317, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 161
HpyF3I CTNAG 3 cut(s) 248, 338, 645
Hsp92II CATG 3 cut(s) 321, 393, 482
KspAI GTTAAC 2 cut(s) 73, 582
LmnI GCTCC 2 cut(s) 161, 328
LpnPI CCDG 5 cut(s) 111, 229, 438, 609, 638
Lsp1109I GCAGC 2 cut(s) 128, 131
MaeIII GTNAC 4 cut(s) 306, 407, 471, 598
MboII GAAGA 2 cut(s) 46, 544
MhlI GDGCHC 2 cut(s) 22, 148
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 7 cut(s) 79, 139, 274, 363, 372, 433, 604
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 2 cut(s) 314, 656
Mox20I TGGCCA 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 185
MscI TGGCCA 1 cut(s) 449
MseI TTAA 6 cut(s) 72, 150, 240, 258, 422, 581
Msp20I TGGCCA 1 cut(s) 449
MspR9I CCNGG 1 cut(s) 653
MvaI CCWGG 1 cut(s) 653
MwoI GCNNNNNNNGC 2 cut(s) 152, 161
NlaIII CATG 3 cut(s) 321, 393, 482
NlaIV GGNNCC 2 cut(s) 636, 657
NmuCI GTSAC 2 cut(s) 306, 598
PceI AGGCCT 1 cut(s) 545
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 5 cut(s) 288, 334, 503, 563, 624
PkrI GCNGC 2 cut(s) 118, 121
PleI GAGTC 2 cut(s) 313, 655
PpsI GAGTC 2 cut(s) 313, 655
PshAI GACNNNNGTC 1 cut(s) 304
Psp6I CCWGG 1 cut(s) 651
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 2 cut(s) 636, 657
RsaI GTAC 3 cut(s) 236, 323, 348
RsaNI GTAC 3 cut(s) 235, 322, 347
SaqAI TTAA 6 cut(s) 72, 150, 240, 258, 422, 581
SatI GCNGC 2 cut(s) 117, 120
SchI GAGTC 2 cut(s) 314, 656
ScrFI CCNGG 1 cut(s) 653
SduI GDGCHC 2 cut(s) 22, 148
SetI ASST 4 cut(s) 124, 157, 348, 400
SmlI CTYRAG 2 cut(s) 302, 399
SmoI CTYRAG 2 cut(s) 302, 399
Sse9I AATT 7 cut(s) 79, 139, 274, 363, 372, 433, 604
SseBI AGGCCT 1 cut(s) 545
SspI AATATT 1 cut(s) 28
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 651
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 501, 506
TasI AATT 7 cut(s) 79, 139, 274, 363, 372, 433, 604
TfiI GAWTC 5 cut(s) 288, 334, 503, 563, 624
Tru1I TTAA 6 cut(s) 72, 150, 240, 258, 422, 581
Tru9I TTAA 6 cut(s) 72, 150, 240, 258, 422, 581
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 2 cut(s) 306, 598
TseI GCWGC 2 cut(s) 116, 119
Tsp45I GTSAC 2 cut(s) 306, 598
TspDTI ATGAA 2 cut(s) 495, 590
TspRI CASTG 1 cut(s) 457
XapI RAATTY 3 cut(s) 79, 363, 604
XmnI GAANNNNTTC 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.