RchiOBHm_Chr6g0280491

Isocitrate dehydrogenase NAD regulatory subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020443
Physical Location & Seq
Reverse (-)
43924457 .. 43925219
763 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25152

Sequence Viewer

Length: 360 bp
ATGGTTGCCACTCCTAATCTTTATGGAAATCTAGTAGCAAATACAGCAGCTGGTATTGCTGGAGGCACTGGTGTCATGCCAGGAGGAAATGTGGGTGCTGATCATGCTGTTTTTGAGCAAGGTGCTTCAGCAGCAAATGTAGGGAATGAAAAGCTATGGGAACAAAAGACTGCCAATCCAGCTGCATTGCTCCTGTCATCAGCCATGATGTTGAGGCATCTCCAGTTTCCCTCATTTGCTGATCGACTTGAAACAGCCGTGAAACGTGTAATTTCAGAAGGCAAGTACCGTAACAAAGATCTTGGTGGAGTGAGCACTACCCAAGAGGTCGTTGACGCCGTTATTTCTCATCTGGACTGA

Protein Analysis

119

Amino Acids

12.38

Weight (kDa)

5.85

Isoelectric Point (pI)

26.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 2 - 114 1.7e-21 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020443)

Species Orthologous Gene IDs
pyrus_communis pycom10g11780 pycom15g20810
rosa_chinensis RchiOBHm_Chr6g0280491
rosa_multiflora Rmu_sc0004211.1_g000030
rosa_samantha Rh6AG243900 Rh6BG246600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 111
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 251
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 3 cut(s) 50, 154, 182
AluI AGCT 3 cut(s) 50, 154, 182
Alw21I GWGCWC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 47, 131, 182
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 3 cut(s) 59, 143, 169
BceAI ACGGC 2 cut(s) 242, 323
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 100
BfaI CTAG 1 cut(s) 32
BglII AGATCT 1 cut(s) 298
BisI GCNGC 3 cut(s) 48, 132, 183
BlsI GCNGC 3 cut(s) 49, 133, 184
Bme1390I CCNGG 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 226
BpmI CTGGAG 2 cut(s) 81, 206
BsaHI GRCGYC 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bse1I ACTGG 2 cut(s) 73, 223
Bse3DI GCAATG 1 cut(s) 185
BseBI CCWGG 1 cut(s) 81
BseMI GCAATG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 73, 223
BseXI GCAGC 3 cut(s) 59, 143, 169
BsiHKAI GWGCWC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 3 cut(s) 100, 241, 298
BsrDI GCAATG 1 cut(s) 185
BsrI ACTGG 2 cut(s) 73, 223
BssMI GATC 3 cut(s) 100, 241, 298
BssNI GRCGYC 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 336
BstKTI GATC 3 cut(s) 103, 244, 301
BstMBI GATC 3 cut(s) 100, 241, 298
BstMWI GCNNNNNNNGC 5 cut(s) 44, 56, 104, 131, 179
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 59, 143, 169
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BtsIMutI CAGTG 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 50
CseI GACGC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 286
CviAII CATG 3 cut(s) 76, 104, 205
CviJI RGCY 5 cut(s) 50, 154, 182, 203, 257
CviKI_1 RGCY 5 cut(s) 50, 154, 182, 203, 257
CviQI GTAC 1 cut(s) 286
DpnI GATC 3 cut(s) 102, 243, 300
DpnII GATC 3 cut(s) 100, 241, 298
Eco57I CTGAAG 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 79
FaeI CATG 3 cut(s) 79, 107, 208
FaiI YATR 5 cut(s) 24, 77, 105, 157, 206
FatI CATG 3 cut(s) 75, 103, 204
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 48, 132, 183
Fsp4HI GCNGC 3 cut(s) 48, 132, 183
FspBI CTAG 1 cut(s) 32
GluI GCNGC 3 cut(s) 48, 132, 183
GsuI CTGGAG 2 cut(s) 81, 206
HgaI GACGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 79, 107, 208
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 56, 104, 131, 179
HpySE526I ACGT 1 cut(s) 265
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 79, 107, 208
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 3 cut(s) 100, 241, 298
LmnI GCTCC 1 cut(s) 195
LpnPI CCDG 9 cut(s) 36, 45, 54, 66, 93, 192, 206, 236, 338
Lsp1109I GCAGC 3 cut(s) 59, 143, 169
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 1 cut(s) 290
MalI GATC 3 cut(s) 102, 243, 300
MboI GATC 3 cut(s) 100, 241, 298
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 1 cut(s) 270
MnlI CCTC 5 cut(s) 56, 77, 207, 241, 319
MspA1I CMGCKG 2 cut(s) 50, 182
MspR9I CCNGG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 5 cut(s) 44, 56, 104, 131, 179
NdeII GATC 3 cut(s) 100, 241, 298
NlaIII CATG 3 cut(s) 79, 107, 208
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PkrI GCNGC 3 cut(s) 49, 133, 184
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 298
PvuII CAGCTG 2 cut(s) 50, 182
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SatI GCNGC 3 cut(s) 48, 132, 183
Sau3AI GATC 3 cut(s) 100, 241, 298
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 317
SetI ASST 6 cut(s) 52, 124, 156, 184, 268, 330
SfaNI GCATC 1 cut(s) 226
Sse9I AATT 1 cut(s) 270
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 268
TaqI TCGA 1 cut(s) 244
TasI AATT 1 cut(s) 270
TscAI CASTG 1 cut(s) 73
TseI GCWGC 3 cut(s) 47, 131, 182
TspDTI ATGAA 1 cut(s) 162
TspRI CASTG 1 cut(s) 73
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.