Rh6BG246600

Isocitrate dehydrogenase NAD regulatory subunit 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0020443
Physical Location & Seq
Reverse (-)
45808344 .. 45826419
18076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG246600.1

Sequence Viewer

Length: 330 bp
ATGCTTGCAAAGACAGCAGCTGGTATTGCTGGAGGCACTGGTGTCATGCCAGGAGGAAATGTGGGTGCTGATCATGCTGTTTTTGAGCAAGGTGCTTCAGCAGCAAATGTAGGGAATGAAAAGCTTTGGGAGCAAAAGACTTCCAATCCAGTTGCATTGCTCCTGTCATCAGCCATGATGTTGAGGCATCTCCAGTTTCCCTCATTTGCTGATCGACTTGAAACAGCCGTGAAACGTGTAATTTCAGAAGGCAAGTACCGTACCAAAGATCTTGGTGGAGTGCTACCCAAGAGGTCGTTGACGCCGTTATTTCTCATCTGGACTGATTGA

Protein Analysis

109

Amino Acids

11.58

Weight (kDa)

9.52

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 2 - 95 4.3e-16 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020443)

Species Orthologous Gene IDs
pyrus_communis pycom10g11780 pycom15g20810
rosa_chinensis RchiOBHm_Chr6g0280491
rosa_multiflora Rmu_sc0004211.1_g000030
rosa_samantha Rh6AG243900 Rh6BG246600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 81
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 2 cut(s) 257, 262
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 2 cut(s) 20, 124
AluI AGCT 2 cut(s) 20, 124
AlwNI CAGNNNCTG 1 cut(s) 20
ApeKI GCWGC 2 cut(s) 17, 101
BbvI GCAGC 2 cut(s) 29, 113
BceAI ACGGC 2 cut(s) 212, 289
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 70
BglII AGATCT 1 cut(s) 268
BisI GCNGC 2 cut(s) 18, 102
BlsI GCNGC 2 cut(s) 19, 103
Bme1390I CCNGG 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 2 cut(s) 51, 176
BsaHI GRCGYC 1 cut(s) 302
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse1I ACTGG 3 cut(s) 43, 149, 193
Bse3DI GCAATG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 155
BseNI ACTGG 3 cut(s) 43, 149, 193
BseXI GCAGC 2 cut(s) 29, 113
Bsp143I GATC 3 cut(s) 70, 211, 268
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 3 cut(s) 43, 149, 193
BssMI GATC 3 cut(s) 70, 211, 268
BssNI GRCGYC 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 6
BstKTI GATC 3 cut(s) 73, 214, 271
BstMBI GATC 3 cut(s) 70, 211, 268
BstMWI GCNNNNNNNGC 5 cut(s) 14, 26, 74, 101, 130
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstV1I GCAGC 2 cut(s) 29, 113
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 20
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 256, 261
CviAII CATG 3 cut(s) 46, 74, 175
CviJI RGCY 4 cut(s) 20, 124, 173, 227
CviKI_1 RGCY 4 cut(s) 20, 124, 173, 227
CviQI GTAC 2 cut(s) 256, 261
DpnI GATC 3 cut(s) 72, 213, 270
DpnII GATC 3 cut(s) 70, 211, 268
Eco57I CTGAAG 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 49
FaeI CATG 3 cut(s) 49, 77, 178
FaiI YATR 3 cut(s) 47, 75, 176
FatI CATG 3 cut(s) 45, 73, 174
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 18, 102
Fsp4HI GCNGC 2 cut(s) 18, 102
GluI GCNGC 2 cut(s) 18, 102
GsuI CTGGAG 2 cut(s) 51, 176
HgaI GACGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 3 cut(s) 49, 77, 178
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 1 cut(s) 300
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 235
HpyCH4V TGCA 2 cut(s) 8, 155
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 26, 74, 101, 130
HpySE526I ACGT 1 cut(s) 235
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 3 cut(s) 49, 77, 178
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 3 cut(s) 70, 211, 268
LmnI GCTCC 2 cut(s) 130, 165
LpnPI CCDG 9 cut(s) 6, 15, 24, 36, 63, 162, 176, 206, 304
Lsp1109I GCAGC 2 cut(s) 29, 113
LweI GCATC 1 cut(s) 196
MaeII ACGT 1 cut(s) 235
MalI GATC 3 cut(s) 72, 213, 270
MboI GATC 3 cut(s) 70, 211, 268
MflI RGATCY 1 cut(s) 268
MluCI AATT 1 cut(s) 240
MnlI CCTC 5 cut(s) 26, 47, 177, 211, 285
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 14, 26, 74, 101, 130
NdeII GATC 3 cut(s) 70, 211, 268
NlaIII CATG 3 cut(s) 49, 77, 178
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PkrI GCNGC 2 cut(s) 19, 103
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 257, 262
RsaNI GTAC 2 cut(s) 256, 261
SatI GCNGC 2 cut(s) 18, 102
Sau3AI GATC 3 cut(s) 70, 211, 268
ScrFI CCNGG 1 cut(s) 51
SetI ASST 5 cut(s) 22, 94, 126, 238, 296
SfaNI GCATC 1 cut(s) 196
Sse9I AATT 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 1 cut(s) 238
TaqI TCGA 1 cut(s) 214
TasI AATT 1 cut(s) 240
TscAI CASTG 1 cut(s) 43
TseI GCWGC 2 cut(s) 17, 101
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.