RchiOBHm_Chr6g0292461

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0018380
Physical Location & Seq
Forward (+)
55352414 .. 55353817
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26245

Sequence Viewer

Length: 1404 bp
ATGAACATAGCTCACGGATTCGTAAGAGGCGGTCTGCATTCGCTAGAAGCCGTTTGCAAGGCAGTCTGCAACTTCTTGTGGCAGCCGGCCGCCGTCGCCAGAGCTGCTTCTCTTGAAGGGCAGATTATTTCTCAGCCACAAAATCTTCTTCCCGCAAAACCAGGTAAATTCGAGAGTGTATTCTTCCTCCTAGATTGGATAACCGGAGGCGGCCGCCGAGGCGGTGGCGCCGATCAGGAGGTAGCCAAGGGGAAAGCAGTCGAAGCAAAGCCTCATCATCCGGACGATTTCCATGTGTGTGGTCCTCGCAATGTGTCACGCCCCAATTGGAGAGACCTGATCAGATCAAGTTGGAACGACGGCAAGTACAAAAGAACTGTGATTGCATGCTTCACACAAGCAATCTACTTGCTCGAGATGGACAGACAAGACAAGAGAGATGCAGCTGCTGCTCTTGCTCCAAAGTGGTGGAAGCCCTTCAAGTATAAACTGACCCGAACTCTTGTTGACGAAAGAGATGGATCCATTTTTGGTGCAATTCTAGAATGGGATCAATCTGCAGCACTTGGTGATTTTGTTGTCATGAGACCAAGCGGCGCATCAAGAGCGGTTTTAGCTCTTAGAGGCACATTGCTGAAGTTCCCGACGATTAGAAGGGACATCGAGGATGACCTTCGGTACGTTGCTTGGGAGAGCTTGAAGGGTTCTGTTAGGTTTGGAGTTGCTTTGGAGGCTTTGAAATCAGTGGCCGAAAAGTATGGAAGTGACAATGTGTGCATTGCTGGCCATTCATTAGGAGCTGGTTTTGCTCTCCAAGTGGGAAAAGCATTAGCTAAACGAGGGATGTATGTAGAGACTCATTTGTTCAATCCGCCTTCGGTTTCGCTAGCAATGACCTTGAGGAATATTAGAGAGAAAGCAGGATTTGTTTGGAAAAGGTTTATGCCAATGCTTCATTTGACGACTGATCAAAGTCATGTTAAGCACAGTGAAGTAATAGTTAGGGATAATGAGTGGACTAGTGGTTTTGGATTGAAGAACTGGATACCAAAATTTTCGAGATTGAAGAATCGCGGTGTAGGTTTGGTAAAATGGTTGCCTCATTTGTATGTGAATAAAAGTGACTACATCTGTTGCTTTTATGCTGACGTGGACGACGGAAGAGAAGGAAACAATGCTGGCGAAAAGGAGAATGAGTGTCGGAATTTAACAAATGGACAAGTAGCTGCGAAGCTGTTTGTGATGTCGAAGCCAAATCAAAGGTTTCTAGAGGCACATGGGTTGCAGCAATGGTGGTCTGATGATTTGCAACTCCAGCTGGCTCTTAATAACAGTAAGCTTATCAGCAGGCAGCTGAGATCCTTGCATAGCCTTCCACGTCCTCAACTAACACTTGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

467

Amino Acids

52.4

Weight (kDa)

9.6

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24630
rosa_chinensis RchiOBHm_Chr6g0292461
rosa_laevigata RLG00000012022
rosa_multiflora Rmu_sc0001354.1_g000024
rosa_roxburghii Rroxscaffold_7G00175040
rosa_rugosa Rorug06G0226800
rosa_samantha Rh6AG340100 Rh6BG347400 Rh6DG340200
rosa_wichuraiana Rw6G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 608
AccII CGCG 1 cut(s) 1074
AccIII TCCGGA 1 cut(s) 280
AclWI GGATC 4 cut(s) 516, 529, 558, 1353
AcoI YGGCCR 4 cut(s) 87, 211, 747, 784
AcsI RAATTY 3 cut(s) 167, 1052, 1204
AcuI CTGAAG 1 cut(s) 656
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 2 cut(s) 368, 680
AgsI TTSAA 7 cut(s) 116, 481, 700, 739, 868, 1036, 1066
AhlI ACTAGT 1 cut(s) 1019
AjiI CACGTC 2 cut(s) 1150, 1379
AjnI CCWGG 1 cut(s) 160
Alw26I GTCTC 3 cut(s) 327, 580, 848
AlwI GGATC 4 cut(s) 516, 529, 558, 1353
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 280
AoxI GGCC 4 cut(s) 87, 211, 747, 784
ApeKI GCWGC 9 cut(s) 82, 104, 443, 446, 449, 560, 1226, 1285, 1351
ApoI RAATTY 3 cut(s) 167, 1052, 1204
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 2 cut(s) 230, 599
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 581
AsuNHI GCTAGC 1 cut(s) 886
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 786
BamHI GGATCC 1 cut(s) 521
BanI GGYRCC 1 cut(s) 227
BbvI GCAGC 9 cut(s) 91, 94, 433, 436, 455, 572, 1213, 1297, 1363
BccI CCATC 2 cut(s) 412, 512
BceAI ACGGC 3 cut(s) 35, 77, 376
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 1038
BclI TGATCA 2 cut(s) 339, 967
BcoDI GTCTC 3 cut(s) 327, 580, 848
BcuI ACTAGT 1 cut(s) 1019
BfaI CTAG 7 cut(s) 44, 191, 542, 887, 1020, 1268, 1398
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 231
BfuI GTATCC 1 cut(s) 1038
BglI GCCNNNNNGGC 1 cut(s) 219
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 413
BmgBI CACGTC 2 cut(s) 1150, 1379
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 229, 523
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 430, 608
BmtI GCTAGC 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 1298
BpuEI CTTGAG 1 cut(s) 919
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 2 cut(s) 327, 580
BsaJI CCNNGG 2 cut(s) 217, 246
BsaWI WCCGGW 2 cut(s) 203, 280
BsaXI ACNNNNNCTCC 2 cut(s) 1181, 1211
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 1046
Bse3DI GCAATG 5 cut(s) 316, 629, 777, 897, 1295
BseAI TCCGGA 1 cut(s) 280
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 217, 246
BseGI GGATG 3 cut(s) 277, 673, 849
BseMI GCAATG 5 cut(s) 316, 629, 777, 897, 1295
BseMII CTCAG 2 cut(s) 146, 1346
BseNI ACTGG 1 cut(s) 1046
BseX3I CGGCCG 2 cut(s) 87, 211
BseXI GCAGC 9 cut(s) 91, 94, 433, 436, 455, 572, 1213, 1297, 1363
Bsh1236I CGCG 1 cut(s) 1074
Bsh1285I CGRYCG 2 cut(s) 90, 214
BshFI GGCC 4 cut(s) 89, 213, 749, 786
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 2 cut(s) 90, 214
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 3 cut(s) 86, 204, 281
BslFI GGGAC 1 cut(s) 671
BsmAI GTCTC 3 cut(s) 327, 580, 848
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 4 cut(s) 89, 213, 749, 786
Bso31I GGTCTC 2 cut(s) 327, 580
BsoBI CYCGRG 1 cut(s) 413
Bsp13I TCCGGA 1 cut(s) 280
Bsp143I GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
BspANI GGCC 4 cut(s) 89, 213, 749, 786
BspCNI CTCAG 2 cut(s) 145, 1347
BspEI TCCGGA 1 cut(s) 280
BspFNI CGCG 1 cut(s) 1074
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 2 cut(s) 229, 523
BspMAI CTGCAG 1 cut(s) 562
BspOI GCTAGC 1 cut(s) 890
BspPI GGATC 4 cut(s) 516, 529, 558, 1353
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 2 cut(s) 327, 580
BsrBI CCGCTC 1 cut(s) 608
BsrDI GCAATG 5 cut(s) 316, 629, 777, 897, 1295
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 1 cut(s) 1046
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 217, 246
BssMI GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 379, 989, 1334
Bst6I CTCTTC 1 cut(s) 1156
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 7 cut(s) 87, 388, 784, 888, 1180, 1320, 1349
BstDEI CTNAG 3 cut(s) 132, 620, 1355
BstF5I GGATG 3 cut(s) 277, 673, 849
BstFNI CGCG 1 cut(s) 1074
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 2 cut(s) 230, 599
BstKTI GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
BstMAI GTCTC 3 cut(s) 327, 580, 848
BstMBI GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
BstMCI CGRYCG 2 cut(s) 90, 214
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 1 cut(s) 1074
BstV1I GCAGC 9 cut(s) 91, 94, 433, 436, 455, 572, 1213, 1297, 1363
BstX2I RGATCY 2 cut(s) 521, 1358
BstXI CCANNNNNNTGG 2 cut(s) 299, 468
BstYI RGATCY 2 cut(s) 521, 1358
BstZI CGGCCG 2 cut(s) 87, 211
BsuI GTATCC 1 cut(s) 1038
BsuRI GGCC 4 cut(s) 89, 213, 749, 786
BtrI CACGTC 2 cut(s) 1150, 1379
BtsCI GGATG 3 cut(s) 277, 673, 849
BtsIMutI CAGTG 2 cut(s) 750, 994
Cac8I GCNNGC 7 cut(s) 87, 388, 784, 888, 1180, 1320, 1349
CaiI CAGNNNCTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 582
CciNI GCGGCCGC 1 cut(s) 211
CfoI GCGC 2 cut(s) 230, 599
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 302
CsiI ACCWGGT 1 cut(s) 160
Csp6I GTAC 2 cut(s) 367, 679
CviAII CATG 5 cut(s) 293, 387, 583, 977, 1277
CviQI GTAC 2 cut(s) 367, 679
DdeI CTNAG 3 cut(s) 132, 620, 1355
DinI GGCGCC 1 cut(s) 229
DpnI GATC 7 cut(s) 234, 341, 346, 523, 552, 969, 1360
DpnII GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
DraIII CACNNNGTG 1 cut(s) 569
EaeI YGGCCR 4 cut(s) 87, 211, 747, 784
EagI CGGCCG 2 cut(s) 87, 211
Eam1104I CTCTTC 1 cut(s) 1156
EarI CTCTTC 1 cut(s) 1156
EciI GGCGGA 1 cut(s) 861
EclXI CGGCCG 2 cut(s) 87, 211
Eco130I CCWWGG 1 cut(s) 246
Eco31I GGTCTC 2 cut(s) 327, 580
Eco47I GGWCC 1 cut(s) 302
Eco52I CGGCCG 2 cut(s) 87, 211
Eco57I CTGAAG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 5 cut(s) 296, 390, 586, 980, 1280
FaqI GGGAC 1 cut(s) 671
FatI CATG 5 cut(s) 292, 386, 582, 976, 1276
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 2 cut(s) 339, 967
FokI GGATG 3 cut(s) 264, 680, 856
FspBI CTAG 7 cut(s) 44, 191, 542, 887, 1020, 1268, 1398
GlaI GCGC 2 cut(s) 229, 598
GsuI CTGGAG 1 cut(s) 1298
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 4 cut(s) 89, 213, 749, 786
HapII CCGG 3 cut(s) 86, 204, 281
HhaI GCGC 2 cut(s) 230, 599
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 296, 390, 586, 980, 1280
Hin6I GCGC 2 cut(s) 228, 597
HinP1I GCGC 2 cut(s) 228, 597
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 1337
HinfI GANTC 3 cut(s) 18, 856, 1069
HpaII CCGG 3 cut(s) 86, 204, 281
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 3 cut(s) 508, 1017, 1153
Hpy188I TCNGA 3 cut(s) 344, 1203, 1300
Hpy8I GTNNAC 3 cut(s) 508, 1017, 1153
Hpy99I CGWCG 4 cut(s) 98, 362, 649, 1160
HpyAV CCTTC 8 cut(s) 110, 487, 648, 683, 694, 885, 1160, 1382
HpyCH4III ACNGT 3 cut(s) 379, 989, 1334
HpyCH4IV ACGT 3 cut(s) 681, 1149, 1378
HpyF3I CTNAG 3 cut(s) 132, 620, 1355
HpySE526I ACGT 3 cut(s) 681, 1149, 1378
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 296, 390, 586, 980, 1280
HspAI GCGC 2 cut(s) 228, 597
KasI GGCGCC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 280
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 2 cut(s) 339, 967
Kzo9I GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
LmnI GCTCC 2 cut(s) 463, 797
Lsp1109I GCAGC 9 cut(s) 91, 94, 433, 436, 455, 572, 1213, 1297, 1363
LweI GCATC 2 cut(s) 430, 608
MabI ACCWGGT 1 cut(s) 160
MaeI CTAG 7 cut(s) 44, 191, 542, 887, 1020, 1268, 1398
MaeII ACGT 3 cut(s) 681, 1149, 1378
MaeIII GTNAC 3 cut(s) 315, 764, 1121
MalI GATC 7 cut(s) 234, 341, 346, 523, 552, 969, 1360
MbiI CCGCTC 1 cut(s) 608
MboI GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
MboII GAAGA 6 cut(s) 137, 140, 175, 1048, 1078, 1173
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 2 cut(s) 521, 1358
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 5 cut(s) 167, 325, 537, 1052, 1204
MluNI TGGCCA 1 cut(s) 786
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 1 cut(s) 850
MmeI TCCRAC 2 cut(s) 332, 1181
Mox20I TGGCCA 1 cut(s) 786
MroI TCCGGA 1 cut(s) 280
MroNI GCCGGC 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 1 cut(s) 786
MseI TTAA 3 cut(s) 981, 1208, 1326
MslI CAYNNNNRTG 2 cut(s) 297, 1107
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 3 cut(s) 446, 1318, 1354
MspI CCGG 3 cut(s) 86, 204, 281
MspR9I CCNGG 1 cut(s) 162
MunI CAATTG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 37
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 1074
NaeI GCCGGC 1 cut(s) 87
NarI GGCGCC 1 cut(s) 228
NdeII GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
NgoMIV GCCGGC 1 cut(s) 85
NheI GCTAGC 1 cut(s) 886
NlaIII CATG 5 cut(s) 296, 390, 586, 980, 1280
NlaIV GGNNCC 2 cut(s) 229, 523
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 3 cut(s) 315, 764, 1121
NotI GCGGCCGC 1 cut(s) 211
NspI RCATGY 1 cut(s) 390
PaeI GCATGC 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 1 cut(s) 582
PctI GAATGC 1 cut(s) 37
PdiI GCCGGC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 18, 1069
PleI GAGTC 1 cut(s) 850
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 850
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 229, 523
PspPI GGNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 521, 1358
PvuII CAGCTG 3 cut(s) 446, 1318, 1354
RsaI GTAC 2 cut(s) 368, 680
RsaNI GTAC 2 cut(s) 367, 679
RseI CAYNNNNRTG 2 cut(s) 297, 1107
SaqAI TTAA 3 cut(s) 981, 1208, 1326
Sau3AI GATC 7 cut(s) 232, 339, 344, 521, 550, 967, 1358
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 850
ScrFI CCNGG 1 cut(s) 162
SexAI ACCWGGT 1 cut(s) 160
SfaNI GCATC 2 cut(s) 430, 608
SfcI CTRYAG 1 cut(s) 558
SfoI GGCGCC 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 297, 1107
SmlI CTYRAG 2 cut(s) 413, 898
SmoI CTYRAG 2 cut(s) 413, 898
SpeI ACTAGT 1 cut(s) 1019
SphI GCATGC 1 cut(s) 390
Sse9I AATT 5 cut(s) 167, 325, 537, 1052, 1204
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 1 cut(s) 907
SspMI CTAG 7 cut(s) 44, 191, 542, 887, 1020, 1268, 1398
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 379, 989, 1334
TaiI ACGT 3 cut(s) 684, 1152, 1381
TaqI TCGA 6 cut(s) 171, 261, 414, 663, 1058, 1247
TasI AATT 5 cut(s) 167, 325, 537, 1052, 1204
TatI WGTACW 1 cut(s) 366
TauI GCSGC 4 cut(s) 92, 213, 216, 597
TfiI GAWTC 2 cut(s) 18, 1069
Tru1I TTAA 3 cut(s) 981, 1208, 1326
Tru9I TTAA 3 cut(s) 981, 1208, 1326
TscAI CASTG 2 cut(s) 750, 994
TseFI GTSAC 3 cut(s) 315, 764, 1121
TseI GCWGC 9 cut(s) 82, 104, 443, 446, 449, 560, 1226, 1285, 1351
Tsp45I GTSAC 3 cut(s) 315, 764, 1121
TspDTI ATGAA 3 cut(s) 17, 780, 944
TspGWI ACGGA 2 cut(s) 30, 1173
TspRI CASTG 2 cut(s) 750, 994
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 3 cut(s) 167, 1052, 1204
XbaI TCTAGA 2 cut(s) 541, 1267
XceI RCATGY 1 cut(s) 390
XhoI CTCGAG 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 7 cut(s) 44, 191, 542, 887, 1020, 1268, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.