RLG00000012022

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018380
Physical Location & Seq
Reverse (-)
14614152 .. 14615588
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012022

Sequence Viewer

Length: 1437 bp
ATGATGAACATAGCTCACGGATTCGTAAGAGGCGGTCTGCATTCGCTAGAAGCCGTTTGCAAGGCAGTCTGCCACTTCGTGTGGCAACCGGCCGCCGTCGCCAGAGCTGCTTCTCTTGAAGGGCAGATTATTTCTCAGCCACAAAATCTTCTTCCCGCAAAACCAGGTAAATTCGAGAGTGTACTCTTCCTCCTAGATTGGATAACCGGAGGCGGCCGCCGCGGCGGTGGTGCCGATCAGGAGGTAGCCAAGGAGAAAGCAGTCGAAGCAAAGCCTCATCATCCGGACGATTTCCATGTGTGTGGTCCTCGCAATGTGTCACGCCCCAGTTGGAGAGACCTGATCAGATCAAGTTGGAACGACGGCAAGTACAAAAGAACTGTGATTGCATGCTTCACACAAGCAATCTACTTGCTCGAGATGGACAGACAAGAGAAGAGAGATGCAGCTGCTGCTCTTGCTCCAAAGTGGTGGAAGCCCTTCAAGTATAAACTGACCCGAACTCTTGTTGACGAAAGAGATGGATCCATTTTTGGTGCAATACTAGAATGGGATCAATCTGCAGCACTTGGTGATTTTGTTGTCATGAGACCAAGCGGCGCATCAAGAGCGGTTTTAGCTCTTAGAGGCACATTGCTGAAGTTCCCGACGATTAGAAGGGACATCGAGGATGACCTTCGGTACGTTGCTTGGGAGAGCTTGAAGGGTTCTGTTAGGTTTGGAGTAGCTTTGGAGGCTTTGAAATCAGTGGCCGAAAAGTATGGAAGTGACAATGTGTGCATTGCTGGCCATTCATTAGGAGCTGGTTTTGCTCTCCAAGTGGGAAAAGCATTCGCTAAACGAGGGGTGTATGTAGAGACTCATTTGTTCAATCCGCCTTCGGTTTCGCTAGCAATGACCTTGAGGAATATTAGAGAGAAAACAGGATTTGTTTGGAAAAGGTTTATGTCAATGCTTCATTTGAGGACTGATCAAAGTCATGTTAAGCACACTGAAGTAATAGTTAGGGATAATGAGTGGACTAGTGGTTTTGGATTGAAGAACTGGATACCAAAATTTTCGGGTTTGAAGAATCGCGGTGTAGGTTTGGTAAAATGGTTGCCTCATTTGTATGTGAATAAAAGTGACTACATCTGTTGCTTTTATGCTGACGTTGACGATGGAAGAGAAGGAAACAATTCTGGCGAAAAGGAGAATGTGTGTCGGAATTTAACAAATGGACAAGTAGCCGCGAAGCTGTTTGTGATGTCGAAGCCAAATCAAAGGTTTCTAGAGGCACATGGGTTACAGCAATGGTGGTCTGATGATTTGCAACTTCAGTTGGCTCTCAATAACAGTAAGCTTATCAGCAGGCAGCTGAGATCCTTGCATAGCCTTCCATGTCCTCAACTAACACTTGCTAATAACAAGGGCGGCTTGTTTTCATTGTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

479

Amino Acids

53.53

Weight (kDa)

9.46

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24630
rosa_chinensis RchiOBHm_Chr6g0292461
rosa_laevigata RLG00000012022
rosa_multiflora Rmu_sc0001354.1_g000024
rosa_roxburghii Rroxscaffold_7G00175040
rosa_rugosa Rorug06G0226800
rosa_samantha Rh6AG340100 Rh6BG347400 Rh6DG340200
rosa_wichuraiana Rw6G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 611
AccII CGCG 3 cut(s) 222, 1077, 1232
AccIII TCCGGA 1 cut(s) 283
AclWI GGATC 4 cut(s) 519, 532, 561, 1356
AcoI YGGCCR 4 cut(s) 90, 214, 750, 787
AcsI RAATTY 3 cut(s) 170, 1055, 1207
AcuI CTGAAG 3 cut(s) 659, 1014, 1301
AdeI CACNNNGTG 2 cut(s) 79, 572
AfaI GTAC 3 cut(s) 183, 371, 683
AgsI TTSAA 7 cut(s) 119, 484, 703, 742, 871, 1039, 1069
AhlI ACTAGT 1 cut(s) 1022
AjnI CCWGG 1 cut(s) 163
Alw26I GTCTC 3 cut(s) 330, 583, 851
AlwI GGATC 4 cut(s) 519, 532, 561, 1356
AlwNI CAGNNNCTG 1 cut(s) 452
Ama87I CYCGRG 1 cut(s) 416
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 4 cut(s) 90, 214, 750, 787
ApeKI GCWGC 6 cut(s) 107, 446, 449, 452, 563, 1354
ApoI RAATTY 3 cut(s) 170, 1055, 1207
Asp700I GAANNNNTTC 2 cut(s) 479, 1177
AspLEI GCGC 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 889
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 789
BamHI GGATCC 1 cut(s) 524
BanI GGYRCC 1 cut(s) 230
BbvI GCAGC 6 cut(s) 94, 436, 439, 458, 575, 1366
BccI CCATC 3 cut(s) 415, 515, 1154
BceAI ACGGC 3 cut(s) 38, 80, 379
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 1 cut(s) 1041
BclI TGATCA 2 cut(s) 342, 970
BcoDI GTCTC 3 cut(s) 330, 583, 851
BcuI ACTAGT 1 cut(s) 1022
BfaI CTAG 6 cut(s) 47, 194, 545, 890, 1023, 1271
BfmI CTRYAG 1 cut(s) 561
BfuI GTATCC 1 cut(s) 1041
BglI GCCNNNNNGGC 1 cut(s) 222
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 232, 526
BmrFI CCNGG 1 cut(s) 165
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 433, 611
BmtI GCTAGC 1 cut(s) 893
BmuI ACTGGG 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 922
BsaI GGTCTC 2 cut(s) 330, 583
BsaJI CCNNGG 2 cut(s) 220, 249
BsaWI WCCGGW 2 cut(s) 206, 283
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 1184, 1214
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 327, 1049
Bse3DI GCAATG 5 cut(s) 319, 632, 780, 900, 1298
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 220, 249
BseGI GGATG 2 cut(s) 280, 676
BseMI GCAATG 5 cut(s) 319, 632, 780, 900, 1298
BseMII CTCAG 2 cut(s) 149, 1349
BseNI ACTGG 2 cut(s) 327, 1049
BseX3I CGGCCG 2 cut(s) 90, 214
BseXI GCAGC 6 cut(s) 94, 436, 439, 458, 575, 1366
Bsh1236I CGCG 3 cut(s) 222, 1077, 1232
Bsh1285I CGRYCG 2 cut(s) 93, 217
BshFI GGCC 4 cut(s) 92, 216, 752, 789
BshNI GGYRCC 1 cut(s) 230
BsiEI CGRYCG 2 cut(s) 93, 217
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 89, 207, 284
BslFI GGGAC 1 cut(s) 674
BsmAI GTCTC 3 cut(s) 330, 583, 851
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 2 cut(s) 40, 830
BsnI GGCC 4 cut(s) 92, 216, 752, 789
Bso31I GGTCTC 2 cut(s) 330, 583
BsoBI CYCGRG 1 cut(s) 416
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
BspANI GGCC 4 cut(s) 92, 216, 752, 789
BspCNI CTCAG 2 cut(s) 148, 1350
BspEI TCCGGA 1 cut(s) 283
BspFNI CGCG 3 cut(s) 222, 1077, 1232
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 232, 526
BspMAI CTGCAG 1 cut(s) 565
BspOI GCTAGC 1 cut(s) 893
BspPI GGATC 4 cut(s) 519, 532, 561, 1356
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 2 cut(s) 330, 583
BsrBI CCGCTC 1 cut(s) 611
BsrDI GCAATG 5 cut(s) 319, 632, 780, 900, 1298
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 2 cut(s) 327, 1049
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 2 cut(s) 220, 249
BssMI GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 382, 1337
Bst6I CTCTTC 3 cut(s) 191, 431, 1159
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 4 cut(s) 391, 787, 891, 1352
BstDEI CTNAG 3 cut(s) 135, 623, 1358
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 2 cut(s) 280, 676
BstFNI CGCG 3 cut(s) 222, 1077, 1232
BstHHI GCGC 1 cut(s) 602
BstKTI GATC 7 cut(s) 238, 345, 350, 527, 556, 973, 1364
BstMAI GTCTC 3 cut(s) 330, 583, 851
BstMBI GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
BstMCI CGRYCG 2 cut(s) 93, 217
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 561
BstUI CGCG 3 cut(s) 222, 1077, 1232
BstV1I GCAGC 6 cut(s) 94, 436, 439, 458, 575, 1366
BstX2I RGATCY 2 cut(s) 524, 1361
BstXI CCANNNNNNTGG 2 cut(s) 302, 471
BstYI RGATCY 2 cut(s) 524, 1361
BstZI CGGCCG 2 cut(s) 90, 214
BsuI GTATCC 1 cut(s) 1041
BsuRI GGCC 4 cut(s) 92, 216, 752, 789
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 280, 676
BtsIMutI CAGTG 2 cut(s) 753, 990
Cac8I GCNNGC 4 cut(s) 391, 787, 891, 1352
CaiI CAGNNNCTG 1 cut(s) 452
CciI TCATGA 1 cut(s) 585
CciNI GCGGCCGC 1 cut(s) 214
CfoI GCGC 1 cut(s) 602
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 305
Cfr42I CCGCGG 1 cut(s) 223
CsiI ACCWGGT 1 cut(s) 163
Csp6I GTAC 3 cut(s) 182, 370, 682
CviAII CATG 6 cut(s) 296, 390, 586, 980, 1280, 1380
CviQI GTAC 3 cut(s) 182, 370, 682
DdeI CTNAG 3 cut(s) 135, 623, 1358
DpnI GATC 7 cut(s) 237, 344, 349, 526, 555, 972, 1363
DpnII GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
DraIII CACNNNGTG 2 cut(s) 79, 572
EaeI YGGCCR 4 cut(s) 90, 214, 750, 787
EagI CGGCCG 2 cut(s) 90, 214
Eam1104I CTCTTC 3 cut(s) 191, 431, 1159
EarI CTCTTC 3 cut(s) 191, 431, 1159
EciI GGCGGA 1 cut(s) 864
EclXI CGGCCG 2 cut(s) 90, 214
Eco130I CCWWGG 1 cut(s) 249
Eco31I GGTCTC 2 cut(s) 330, 583
Eco47I GGWCC 1 cut(s) 305
Eco52I CGGCCG 2 cut(s) 90, 214
Eco57I CTGAAG 3 cut(s) 659, 1014, 1301
Eco88I CYCGRG 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 6 cut(s) 299, 393, 589, 983, 1283, 1383
FalI AAGNNNNNCTT 2 cut(s) 1400, 1432
FaqI GGGAC 1 cut(s) 674
FatI CATG 6 cut(s) 295, 389, 585, 979, 1279, 1379
FauI CCCGC 1 cut(s) 163
FbaI TGATCA 2 cut(s) 342, 970
FokI GGATG 2 cut(s) 267, 683
FspBI CTAG 6 cut(s) 47, 194, 545, 890, 1023, 1271
GlaI GCGC 1 cut(s) 601
HaeIII GGCC 4 cut(s) 92, 216, 752, 789
HapII CCGG 3 cut(s) 89, 207, 284
HhaI GCGC 1 cut(s) 602
Hin1II CATG 6 cut(s) 299, 393, 589, 983, 1283, 1383
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HincII GTYRAC 2 cut(s) 511, 1156
HindII GTYRAC 2 cut(s) 511, 1156
HindIII AAGCTT 1 cut(s) 1340
HinfI GANTC 3 cut(s) 21, 859, 1072
HpaII CCGG 3 cut(s) 89, 207, 284
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 4 cut(s) 182, 511, 1020, 1156
Hpy188I TCNGA 3 cut(s) 347, 1206, 1303
Hpy188III TCNNGA 9 cut(s) 116, 175, 239, 284, 418, 586, 606, 646, 1271
Hpy8I GTNNAC 4 cut(s) 182, 511, 1020, 1156
Hpy99I CGWCG 3 cut(s) 101, 365, 652
HpyAV CCTTC 8 cut(s) 113, 490, 651, 686, 697, 888, 1163, 1385
HpyCH4III ACNGT 2 cut(s) 382, 1337
HpyCH4IV ACGT 2 cut(s) 684, 1152
HpyCH4V TGCA 9 cut(s) 40, 60, 389, 446, 539, 563, 780, 1312, 1369
HpyF3I CTNAG 3 cut(s) 135, 623, 1358
HpySE526I ACGT 2 cut(s) 684, 1152
Hsp92II CATG 6 cut(s) 299, 393, 589, 983, 1283, 1383
HspAI GCGC 1 cut(s) 600
Kpn2I TCCGGA 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 342, 970
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
LmnI GCTCC 2 cut(s) 466, 800
Lsp1109I GCAGC 6 cut(s) 94, 436, 439, 458, 575, 1366
LweI GCATC 2 cut(s) 433, 611
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 6 cut(s) 47, 194, 545, 890, 1023, 1271
MaeII ACGT 2 cut(s) 684, 1152
MaeIII GTNAC 4 cut(s) 318, 767, 1124, 1284
MalI GATC 7 cut(s) 237, 344, 349, 526, 555, 972, 1363
MbiI CCGCTC 1 cut(s) 611
MboI GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
MboII GAAGA 7 cut(s) 140, 143, 178, 448, 1051, 1081, 1176
MflI RGATCY 2 cut(s) 524, 1361
MlsI TGGCCA 1 cut(s) 789
MluCI AATT 4 cut(s) 170, 1055, 1177, 1207
MluNI TGGCCA 1 cut(s) 789
MlyI GAGTC 1 cut(s) 853
MmeI TCCRAC 3 cut(s) 311, 335, 1184
Mox20I TGGCCA 1 cut(s) 789
MroI TCCGGA 1 cut(s) 283
MroXI GAANNNNTTC 2 cut(s) 479, 1177
MscI TGGCCA 1 cut(s) 789
MseI TTAA 3 cut(s) 984, 1211, 1435
MslI CAYNNNNRTG 2 cut(s) 300, 1110
Msp20I TGGCCA 1 cut(s) 789
MspA1I CMGCKG 3 cut(s) 222, 449, 1357
MspI CCGG 3 cut(s) 89, 207, 284
MspR9I CCNGG 1 cut(s) 165
Mva1269I GAATGC 2 cut(s) 40, 830
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 3 cut(s) 222, 1077, 1232
NdeII GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
NheI GCTAGC 1 cut(s) 889
NlaIII CATG 6 cut(s) 299, 393, 589, 983, 1283, 1383
NlaIV GGNNCC 2 cut(s) 232, 526
NmuCI GTSAC 3 cut(s) 318, 767, 1124
NotI GCGGCCGC 1 cut(s) 214
NspI RCATGY 1 cut(s) 393
PaeI GCATGC 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 416
PagI TCATGA 1 cut(s) 585
PctI GAATGC 2 cut(s) 40, 830
PdmI GAANNNNTTC 2 cut(s) 479, 1177
PfeI GAWTC 2 cut(s) 21, 1072
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 232, 526
PspPI GGNCC 1 cut(s) 305
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 2 cut(s) 524, 1361
PvuII CAGCTG 2 cut(s) 449, 1357
RsaI GTAC 3 cut(s) 183, 371, 683
RsaNI GTAC 3 cut(s) 182, 370, 682
RseI CAYNNNNRTG 2 cut(s) 300, 1110
SacII CCGCGG 1 cut(s) 223
SaqAI TTAA 3 cut(s) 984, 1211, 1435
Sau3AI GATC 7 cut(s) 235, 342, 347, 524, 553, 970, 1361
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 1 cut(s) 165
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 2 cut(s) 433, 611
SfcI CTRYAG 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 416
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SinI GGWCC 1 cut(s) 305
SlaI CTCGAG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 300, 1110
SmlI CTYRAG 2 cut(s) 416, 901
SmoI CTYRAG 2 cut(s) 416, 901
SpeI ACTAGT 1 cut(s) 1022
SphI GCATGC 1 cut(s) 393
Sse9I AATT 4 cut(s) 170, 1055, 1177, 1207
SspI AATATT 1 cut(s) 910
SspMI CTAG 6 cut(s) 47, 194, 545, 890, 1023, 1271
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 382, 1337
TaiI ACGT 2 cut(s) 687, 1155
TaqI TCGA 5 cut(s) 174, 264, 417, 666, 1250
TasI AATT 4 cut(s) 170, 1055, 1177, 1207
TatI WGTACW 2 cut(s) 181, 369
TauI GCSGC 8 cut(s) 95, 216, 219, 222, 225, 600, 1232, 1416
TfiI GAWTC 2 cut(s) 21, 1072
Tru1I TTAA 3 cut(s) 984, 1211, 1435
Tru9I TTAA 3 cut(s) 984, 1211, 1435
TscAI CASTG 2 cut(s) 753, 997
TseFI GTSAC 3 cut(s) 318, 767, 1124
TseI GCWGC 6 cut(s) 107, 446, 449, 452, 563, 1354
Tsp45I GTSAC 3 cut(s) 318, 767, 1124
TspDTI ATGAA 4 cut(s) 20, 783, 947, 1413
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 2 cut(s) 753, 997
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 3 cut(s) 170, 1055, 1207
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 416
XmnI GAANNNNTTC 2 cut(s) 479, 1177
XspI CTAG 6 cut(s) 47, 194, 545, 890, 1023, 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.