Rh6AG340100

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018380
Physical Location & Seq
Forward (+)
53980487 .. 53981881
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG340100.1

Sequence Viewer

Length: 1395 bp
ATGATGAACGTAGCTCACGGATTCGTAAGAGGCGGTCTGCATTCGCTAGAAGCCGTTTGCAAGGCAGTCTGCCACTTCTTGTGGCAGCCGGCCGCCGTCGCCAGAGCTGCTTCTCTTGAAGGGCAGATTATTTCTCAGCCACAAAATCTTCTTCCCGCAAAACCAGGTAAATTCGAGAGTGTATTCTTCCTCCTAGATTGGATAACCGGAGGCGGTGGCGCCGATCAGGAGGTAGCCAAGGAGAAAGCAGTCGAAGCAAAGCCTCATCATCCGGACGATTTCCATGTGTGTGGTCCTCGCAATGTGTCACGCCCCAATTGGAGAGACCTGATCAGATCAAGTTGGAACGACGGCAAGTACAAAAGAACTGTGATTGCATGCTTCACACAAGCAATCTACTTGCTCGAGATGGACAGACAAGAGAAGAGAGATGCAGCTGCTGCTCTTGCTCCAAAGTGGTGGAAGCCCTTCAAGTATAAACTGACCCGAACTCTTGTTGACGAAAGAGATGGATCCATTTTTGGTGCAATTCTAGAATGGGATCAATCTGCAGCACTTGGTGATTTTGTTGTCATGAGACCAAGCGGCGCATCAAGAGCGGTTTTAGCTCTTAGAGGCACATTGCTGAAGTTCCCGACGATTAGAAGGGACATCGAGGATGACCTTCGGTACGTTGCTTGGGAGAGCTTGAAGGGTTCTGTTAGGTTTGGAGTAGCTTTGGAGGCTTTGAAATCAGTGGCCGAAAAGTATGGAAGTGACAATGTGTGCATTGCTGGCCATTCATTAGGAGCTGGTTTTGCTCTCCAAGTGGGAAAAGCATTAGCTAAACGAGGGATGTATGTAGAGACTCATTTGTTCAATCCGCCTTCGGTTTCGCTAGCAATGACCTTGAGGAATATTAGAGAGAAAGCAGGATTTGTTTGGAAAAGGTTTATGCCAATGCTTCATTTGAGGACTGATCAAAGTCATGTTAAGCACAGTGAAGTAATAGTTAGGGATAATGAGTGGACTAGTGGTTTTGGATTGAAGAACTGGATACCAAAATTTTCGAGTTTGAAGAATCGCGGTGTAGGTTTGGTAAAATGGTTGCCTCATTTGTATGTGAATAAAAGTGACTACATCTGTTGCTTTTATGCTGACGTGGACGACGGAAGAGAAGGAAGCAATGCTGGCGAAAAGGAGAATGTGTGTCGGAATTTAACAAATGGACAAGTAGCTGCGAAGCTGTTTGTGATGTCGAAGCCAAATCAAAGGTTTCTAGAGGCACATGGGTTACAGCAATGGTGGTCTGATGATTTGCAACTCCAGCTGGCTCTTAATAACAGTAAGCTTATCAGCAGGCAGCTGAGATCCTTGCATAGCCTTCCACGTCCTCAACTAACACTTGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

464

Amino Acids

52.13

Weight (kDa)

9.51

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24630
rosa_chinensis RchiOBHm_Chr6g0292461
rosa_laevigata RLG00000012022
rosa_multiflora Rmu_sc0001354.1_g000024
rosa_roxburghii Rroxscaffold_7G00175040
rosa_rugosa Rorug06G0226800
rosa_samantha Rh6AG340100 Rh6BG347400 Rh6DG340200
rosa_wichuraiana Rw6G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccBSI CCGCTC 1 cut(s) 599
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 8 cut(s) 33, 93, 156, 213, 585, 599, 863, 1065
AclWI GGATC 4 cut(s) 507, 520, 549, 1344
AcoI YGGCCR 3 cut(s) 90, 738, 775
AcsI RAATTY 3 cut(s) 170, 1043, 1195
AcuI CTGAAG 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 2 cut(s) 359, 671
AgsI TTSAA 7 cut(s) 119, 472, 691, 730, 859, 1027, 1057
AhlI ACTAGT 1 cut(s) 1010
AjiI CACGTC 2 cut(s) 1141, 1370
AjnI CCWGG 1 cut(s) 163
Alw26I GTCTC 3 cut(s) 318, 571, 839
AlwI GGATC 4 cut(s) 507, 520, 549, 1344
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 404
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 3 cut(s) 90, 738, 775
ApeKI GCWGC 8 cut(s) 85, 107, 434, 437, 440, 551, 1217, 1342
ApoI RAATTY 3 cut(s) 170, 1043, 1195
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 2 cut(s) 221, 590
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 572
AsuNHI GCTAGC 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 777
BamHI GGATCC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 218
BbvI GCAGC 8 cut(s) 94, 97, 424, 427, 446, 563, 1204, 1354
BccI CCATC 2 cut(s) 403, 503
BceAI ACGGC 3 cut(s) 38, 80, 367
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 1 cut(s) 1029
BclI TGATCA 2 cut(s) 330, 958
BcoDI GTCTC 3 cut(s) 318, 571, 839
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 7 cut(s) 47, 194, 533, 878, 1011, 1259, 1389
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 222
BfuI GTATCC 1 cut(s) 1029
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 2 cut(s) 1141, 1370
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 220, 514
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 421, 599
BmtI GCTAGC 1 cut(s) 881
BpmI CTGGAG 1 cut(s) 1289
BpuEI CTTGAG 1 cut(s) 910
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 318, 571
BsaJI CCNNGG 1 cut(s) 237
BsaWI WCCGGW 2 cut(s) 206, 271
BsaXI ACNNNNNCTCC 2 cut(s) 1172, 1202
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 1037
Bse3DI GCAATG 6 cut(s) 307, 620, 768, 888, 1171, 1286
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 3 cut(s) 268, 664, 840
BseMI GCAATG 6 cut(s) 307, 620, 768, 888, 1171, 1286
BseMII CTCAG 2 cut(s) 149, 1337
BseNI ACTGG 1 cut(s) 1037
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 8 cut(s) 94, 97, 424, 427, 446, 563, 1204, 1354
Bsh1236I CGCG 1 cut(s) 1065
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 92, 740, 777
BshNI GGYRCC 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 3 cut(s) 89, 207, 272
BslFI GGGAC 1 cut(s) 662
BsmAI GTCTC 3 cut(s) 318, 571, 839
BsmFI GGGAC 1 cut(s) 662
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 3 cut(s) 92, 740, 777
Bso31I GGTCTC 2 cut(s) 318, 571
BsoBI CYCGRG 1 cut(s) 404
Bsp13I TCCGGA 1 cut(s) 271
Bsp143I GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
BspACI CCGC 8 cut(s) 33, 93, 156, 213, 585, 599, 863, 1065
BspANI GGCC 3 cut(s) 92, 740, 777
BspCNI CTCAG 2 cut(s) 148, 1338
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 1 cut(s) 1065
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 220, 514
BspMAI CTGCAG 1 cut(s) 553
BspOI GCTAGC 1 cut(s) 881
BspPI GGATC 4 cut(s) 507, 520, 549, 1344
BspT107I GGYRCC 1 cut(s) 218
BspTNI GGTCTC 2 cut(s) 318, 571
BsrBI CCGCTC 1 cut(s) 599
BsrDI GCAATG 6 cut(s) 307, 620, 768, 888, 1171, 1286
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 3 cut(s) 370, 980, 1325
Bst6I CTCTTC 2 cut(s) 419, 1147
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 440
BstC8I GCNNGC 7 cut(s) 90, 379, 775, 879, 1171, 1311, 1340
BstDEI CTNAG 3 cut(s) 135, 611, 1346
BstF5I GGATG 3 cut(s) 268, 664, 840
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 590
BstKTI GATC 7 cut(s) 226, 333, 338, 515, 544, 961, 1352
BstMAI GTCTC 3 cut(s) 318, 571, 839
BstMBI GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
BstMCI CGRYCG 1 cut(s) 93
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 8 cut(s) 94, 97, 424, 427, 446, 563, 1204, 1354
BstX2I RGATCY 2 cut(s) 512, 1349
BstXI CCANNNNNNTGG 2 cut(s) 290, 459
BstYI RGATCY 2 cut(s) 512, 1349
BstZI CGGCCG 1 cut(s) 90
BsuI GTATCC 1 cut(s) 1029
BsuRI GGCC 3 cut(s) 92, 740, 777
BtrI CACGTC 2 cut(s) 1141, 1370
BtsCI GGATG 3 cut(s) 268, 664, 840
BtsIMutI CAGTG 2 cut(s) 741, 985
Cac8I GCNNGC 7 cut(s) 90, 379, 775, 879, 1171, 1311, 1340
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 1 cut(s) 573
CfoI GCGC 2 cut(s) 221, 590
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 293
CsiI ACCWGGT 1 cut(s) 163
Csp6I GTAC 2 cut(s) 358, 670
CviAII CATG 5 cut(s) 284, 378, 574, 968, 1268
CviQI GTAC 2 cut(s) 358, 670
DdeI CTNAG 3 cut(s) 135, 611, 1346
DinI GGCGCC 1 cut(s) 220
DpnI GATC 7 cut(s) 225, 332, 337, 514, 543, 960, 1351
DpnII GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
DraIII CACNNNGTG 1 cut(s) 560
EaeI YGGCCR 3 cut(s) 90, 738, 775
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 419, 1147
EarI CTCTTC 2 cut(s) 419, 1147
EciI GGCGGA 1 cut(s) 852
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 237
Eco31I GGTCTC 2 cut(s) 318, 571
Eco47I GGWCC 1 cut(s) 293
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 647
Eco88I CYCGRG 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 5 cut(s) 287, 381, 577, 971, 1271
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 283, 377, 573, 967, 1267
FauI CCCGC 1 cut(s) 163
FbaI TGATCA 2 cut(s) 330, 958
FokI GGATG 3 cut(s) 255, 671, 847
FspBI CTAG 7 cut(s) 47, 194, 533, 878, 1011, 1259, 1389
GlaI GCGC 2 cut(s) 220, 589
GsuI CTGGAG 1 cut(s) 1289
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 3 cut(s) 92, 740, 777
HapII CCGG 3 cut(s) 89, 207, 272
HhaI GCGC 2 cut(s) 221, 590
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 5 cut(s) 287, 381, 577, 971, 1271
Hin6I GCGC 2 cut(s) 219, 588
HinP1I GCGC 2 cut(s) 219, 588
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 1328
HinfI GANTC 3 cut(s) 21, 847, 1060
HpaII CCGG 3 cut(s) 89, 207, 272
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 3 cut(s) 499, 1008, 1144
Hpy188I TCNGA 3 cut(s) 335, 1194, 1291
Hpy8I GTNNAC 3 cut(s) 499, 1008, 1144
Hpy99I CGWCG 4 cut(s) 101, 353, 640, 1151
HpyAV CCTTC 8 cut(s) 113, 478, 639, 674, 685, 876, 1151, 1373
HpyCH4III ACNGT 3 cut(s) 370, 980, 1325
HpyCH4IV ACGT 4 cut(s) 9, 672, 1140, 1369
HpyCH4V TGCA 9 cut(s) 40, 60, 377, 434, 527, 551, 768, 1300, 1357
HpyF3I CTNAG 3 cut(s) 135, 611, 1346
HpySE526I ACGT 4 cut(s) 9, 672, 1140, 1369
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 5 cut(s) 287, 381, 577, 971, 1271
HspAI GCGC 2 cut(s) 219, 588
KasI GGCGCC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 271
KroI GCCGGC 1 cut(s) 88
KroNI GCCGGC 1 cut(s) 90
Ksp22I TGATCA 2 cut(s) 330, 958
Kzo9I GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
LmnI GCTCC 2 cut(s) 454, 788
Lsp1109I GCAGC 8 cut(s) 94, 97, 424, 427, 446, 563, 1204, 1354
LweI GCATC 2 cut(s) 421, 599
MabI ACCWGGT 1 cut(s) 163
MaeI CTAG 7 cut(s) 47, 194, 533, 878, 1011, 1259, 1389
MaeII ACGT 4 cut(s) 9, 672, 1140, 1369
MaeIII GTNAC 4 cut(s) 306, 755, 1112, 1272
MalI GATC 7 cut(s) 225, 332, 337, 514, 543, 960, 1351
MbiI CCGCTC 1 cut(s) 599
MboI GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
MboII GAAGA 7 cut(s) 140, 143, 178, 436, 1039, 1069, 1164
MfeI CAATTG 1 cut(s) 316
MflI RGATCY 2 cut(s) 512, 1349
MlsI TGGCCA 1 cut(s) 777
MluCI AATT 5 cut(s) 170, 316, 528, 1043, 1195
MluNI TGGCCA 1 cut(s) 777
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 2 cut(s) 323, 1172
Mox20I TGGCCA 1 cut(s) 777
MroI TCCGGA 1 cut(s) 271
MroNI GCCGGC 1 cut(s) 88
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 777
MseI TTAA 3 cut(s) 972, 1199, 1317
MslI CAYNNNNRTG 2 cut(s) 288, 1098
Msp20I TGGCCA 1 cut(s) 777
MspA1I CMGCKG 3 cut(s) 437, 1309, 1345
MspI CCGG 3 cut(s) 89, 207, 272
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 1065
NaeI GCCGGC 1 cut(s) 90
NarI GGCGCC 1 cut(s) 219
NdeII GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
NgoMIV GCCGGC 1 cut(s) 88
NheI GCTAGC 1 cut(s) 877
NlaIII CATG 5 cut(s) 287, 381, 577, 971, 1271
NlaIV GGNNCC 2 cut(s) 220, 514
NmuCI GTSAC 3 cut(s) 306, 755, 1112
NspI RCATGY 1 cut(s) 381
PaeI GCATGC 1 cut(s) 381
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 573
PctI GAATGC 1 cut(s) 40
PdiI GCCGGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 21, 1060
PleI GAGTC 1 cut(s) 841
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 220, 514
PspPI GGNCC 1 cut(s) 293
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 2 cut(s) 512, 1349
PvuII CAGCTG 3 cut(s) 437, 1309, 1345
RsaI GTAC 2 cut(s) 359, 671
RsaNI GTAC 2 cut(s) 358, 670
RseI CAYNNNNRTG 2 cut(s) 288, 1098
SaqAI TTAA 3 cut(s) 972, 1199, 1317
Sau3AI GATC 7 cut(s) 223, 330, 335, 512, 541, 958, 1349
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 1 cut(s) 165
SexAI ACCWGGT 1 cut(s) 163
SfaNI GCATC 2 cut(s) 421, 599
SfcI CTRYAG 1 cut(s) 549
SfoI GGCGCC 1 cut(s) 220
Sfr274I CTCGAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 288, 1098
SmlI CTYRAG 2 cut(s) 404, 889
SmoI CTYRAG 2 cut(s) 404, 889
SpeI ACTAGT 1 cut(s) 1010
SphI GCATGC 1 cut(s) 381
Sse9I AATT 5 cut(s) 170, 316, 528, 1043, 1195
SsiI CCGC 8 cut(s) 33, 93, 156, 213, 585, 599, 863, 1065
SspDI GGCGCC 1 cut(s) 218
SspI AATATT 1 cut(s) 898
SspMI CTAG 7 cut(s) 47, 194, 533, 878, 1011, 1259, 1389
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 3 cut(s) 370, 980, 1325
TaiI ACGT 4 cut(s) 12, 675, 1143, 1372
TaqI TCGA 6 cut(s) 174, 252, 405, 654, 1049, 1238
TasI AATT 5 cut(s) 170, 316, 528, 1043, 1195
TatI WGTACW 1 cut(s) 357
TauI GCSGC 2 cut(s) 95, 588
TfiI GAWTC 2 cut(s) 21, 1060
Tru1I TTAA 3 cut(s) 972, 1199, 1317
Tru9I TTAA 3 cut(s) 972, 1199, 1317
TscAI CASTG 2 cut(s) 741, 985
TseFI GTSAC 3 cut(s) 306, 755, 1112
TseI GCWGC 8 cut(s) 85, 107, 434, 437, 440, 551, 1217, 1342
Tsp45I GTSAC 3 cut(s) 306, 755, 1112
TspDTI ATGAA 3 cut(s) 20, 771, 935
TspGWI ACGGA 2 cut(s) 33, 1164
TspRI CASTG 2 cut(s) 741, 985
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 3 cut(s) 170, 1043, 1195
XbaI TCTAGA 2 cut(s) 532, 1258
XceI RCATGY 1 cut(s) 381
XhoI CTCGAG 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 7 cut(s) 47, 194, 533, 878, 1011, 1259, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.