Rw6G029610

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018380
Physical Location & Seq
Forward (+)
53684483 .. 53685886
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G029610.1

Sequence Viewer

Length: 1368 bp
ATGAACATAGCTCACGGATTCGTAAGAGGCGGTCTGCATTCGCTAGAAGCCGTTTGCAAGGCAGTCTGCCACTTCTTGTGGCAGCCGGCCGCCGTCGCCAGAGCTGCTTCTCTTGAAGGGCAGATTATTTCTCAGCCACAAAATCTTCTTCCCGCAAAACCAGGTAAATTCGAGAGTGTATTCTTCCTCCTAGATTGGATAACCGGAGGCGGCCGCCGAGGCGGTGGCGCCGATCAGGAGGTAGCCAAGGAGAAAGCAGTCGAAGCAAAGTCTCATCATCCGGACGATTTCCATGTGTGTGGTCCTCGCAATGTTTGGAACGACGGCAAGTACAAAAGAACTGTGATTGCATGCTTCACACAAGCAATCTACTTGCTCGAGATGGACAGACAAGAGAAGAGAGATGCAGCTGCTGCTCTTGCTCCAAAGTGGTGGAAGCCCTTCAAGTATAAACTGACCCGAACTCTTGTTGACGAAAGAGATGGATCCATTTTTGGTGCAATTCTAGAATGGGATCAATCTGCAGCACTTGGTGATTTTGTTGTCATGAGACCAAGCGGCGCATCAAGAGCGGTTTTAGCTCTTAGAGGCACATTGCTGAAGTTCCCGACGATTAGAAGGGACATCGAGGATGACCTTCGGTACGTTGCTTGGGAGAGCTTGAAGGGTTCTGTTAGGTTTGGAGTAGCTTTGGAGGCTTTGAAATCAGTGGCCGAAAAGTATGGAAGTGACAATGTGTGCATTGCTGGCCATTCATTAGGAGCTGGTTTTGCTCTCCAAGTGGGAAAAGCATTAGCTAAACGAGGGATGTATGTAGAGACTCATTTGTTCAATCCGCCTTCGGTTTCGCTAGCAATGACCTTGAGGAATATTAGAGAGAAAGCAGGATTTGTTTGGAAAAGGTTTATGCCAATGCTTCATTTGAGGACTGATCAAAGTCATGTTAAGCACAGTGAAGTAATAGTTAGGGATAATGAGTGGACTAGTGGTTTTGGATTGAAGAACTGGATACCAAAATTTTCGAGTTTGAAGAATCGCGGTGTAGGTTTGGTAAAATGGTTGCCTCATTTGTATGTGAATAAAAGTGACTACATCTGTTGCTTTTATGCTGACGTGGACGACGGAAGAGAAGGAAACAATGCTGGCGAAAAGGAGAATGTGTATCGGAATTTAACAAATGGACAAGTAGCTGCGAAGCTGTTTGTGATGTCGAAGCCAAATCAAAGGTTTCTAGAGGCACATGGGTTACAGCAATGGTGGTCTGATGATTTGCAACTCCAGCTGGCTCTTAATAACAGTAAGCTTATCAGCAGGCAGCTGAGATCCTTGCATAGCCTTCCACGTCCTCAACTAACACTTGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

455

Amino Acids

51.05

Weight (kDa)

9.54

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24630
rosa_chinensis RchiOBHm_Chr6g0292461
rosa_laevigata RLG00000012022
rosa_multiflora Rmu_sc0001354.1_g000024
rosa_roxburghii Rroxscaffold_7G00175040
rosa_rugosa Rorug06G0226800
rosa_samantha Rh6AG340100 Rh6BG347400 Rh6DG340200
rosa_wichuraiana Rw6G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 572
AccII CGCG 1 cut(s) 1038
AccIII TCCGGA 1 cut(s) 280
AclWI GGATC 4 cut(s) 480, 493, 522, 1317
AcoI YGGCCR 4 cut(s) 87, 211, 711, 748
AcsI RAATTY 3 cut(s) 167, 1016, 1168
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 1 cut(s) 228
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 2 cut(s) 332, 644
AgsI TTSAA 7 cut(s) 116, 445, 664, 703, 832, 1000, 1030
AhlI ACTAGT 1 cut(s) 983
AjiI CACGTC 2 cut(s) 1114, 1343
AjnI CCWGG 1 cut(s) 160
Alw26I GTCTC 3 cut(s) 276, 544, 812
AlwI GGATC 4 cut(s) 480, 493, 522, 1317
AlwNI CAGNNNCTG 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 280
AoxI GGCC 4 cut(s) 87, 211, 711, 748
ApeKI GCWGC 8 cut(s) 82, 104, 407, 410, 413, 524, 1190, 1315
ApoI RAATTY 3 cut(s) 167, 1016, 1168
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 2 cut(s) 230, 563
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 545
AsuNHI GCTAGC 1 cut(s) 850
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 750
BamHI GGATCC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 227
BbvI GCAGC 8 cut(s) 91, 94, 397, 400, 419, 536, 1177, 1327
BccI CCATC 2 cut(s) 376, 476
BceAI ACGGC 3 cut(s) 35, 77, 340
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 1002
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 3 cut(s) 276, 544, 812
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 7 cut(s) 44, 191, 506, 851, 984, 1232, 1362
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 231
BfuI GTATCC 1 cut(s) 1002
BglI GCCNNNNNGGC 1 cut(s) 219
Bme1390I CCNGG 1 cut(s) 162
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 2 cut(s) 1114, 1343
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 229, 487
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 394, 572
BmtI GCTAGC 1 cut(s) 854
BpmI CTGGAG 1 cut(s) 1262
BpuEI CTTGAG 1 cut(s) 883
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 217, 246
BsaWI WCCGGW 2 cut(s) 203, 280
BsaXI ACNNNNNCTCC 2 cut(s) 1145, 1175
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 1010
Bse3DI GCAATG 5 cut(s) 316, 593, 741, 861, 1259
BseAI TCCGGA 1 cut(s) 280
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 217, 246
BseGI GGATG 3 cut(s) 277, 637, 813
BseMI GCAATG 5 cut(s) 316, 593, 741, 861, 1259
BseMII CTCAG 2 cut(s) 146, 1310
BseNI ACTGG 1 cut(s) 1010
BseX3I CGGCCG 2 cut(s) 87, 211
BseXI GCAGC 8 cut(s) 91, 94, 397, 400, 419, 536, 1177, 1327
Bsh1236I CGCG 1 cut(s) 1038
Bsh1285I CGRYCG 2 cut(s) 90, 214
BshFI GGCC 4 cut(s) 89, 213, 713, 750
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 2 cut(s) 90, 214
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 3 cut(s) 86, 204, 281
BslFI GGGAC 1 cut(s) 635
BsmAI GTCTC 3 cut(s) 276, 544, 812
BsmFI GGGAC 1 cut(s) 635
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 4 cut(s) 89, 213, 713, 750
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 280
Bsp143I GATC 5 cut(s) 232, 485, 514, 931, 1322
BspANI GGCC 4 cut(s) 89, 213, 713, 750
BspCNI CTCAG 2 cut(s) 145, 1311
BspEI TCCGGA 1 cut(s) 280
BspFNI CGCG 1 cut(s) 1038
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 229, 487
BspMAI CTGCAG 1 cut(s) 526
BspOI GCTAGC 1 cut(s) 854
BspPI GGATC 4 cut(s) 480, 493, 522, 1317
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 572
BsrDI GCAATG 5 cut(s) 316, 593, 741, 861, 1259
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 1 cut(s) 1010
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 217, 246
BssMI GATC 5 cut(s) 232, 485, 514, 931, 1322
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 343, 953, 1298
Bst6I CTCTTC 2 cut(s) 392, 1120
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 7 cut(s) 87, 352, 748, 852, 1144, 1284, 1313
BstDEI CTNAG 3 cut(s) 132, 584, 1319
BstF5I GGATG 3 cut(s) 277, 637, 813
BstFNI CGCG 1 cut(s) 1038
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 2 cut(s) 230, 563
BstKTI GATC 5 cut(s) 235, 488, 517, 934, 1325
BstMAI GTCTC 3 cut(s) 276, 544, 812
BstMBI GATC 5 cut(s) 232, 485, 514, 931, 1322
BstMCI CGRYCG 2 cut(s) 90, 214
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 8 cut(s) 91, 94, 397, 400, 419, 536, 1177, 1327
BstX2I RGATCY 2 cut(s) 485, 1322
BstXI CCANNNNNNTGG 2 cut(s) 299, 432
BstYI RGATCY 2 cut(s) 485, 1322
BstZI CGGCCG 2 cut(s) 87, 211
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 4 cut(s) 89, 213, 713, 750
BtrI CACGTC 2 cut(s) 1114, 1343
BtsCI GGATG 3 cut(s) 277, 637, 813
BtsIMutI CAGTG 2 cut(s) 714, 958
Cac8I GCNNGC 7 cut(s) 87, 352, 748, 852, 1144, 1284, 1313
CaiI CAGNNNCTG 1 cut(s) 413
CciI TCATGA 1 cut(s) 546
CciNI GCGGCCGC 1 cut(s) 211
CfoI GCGC 2 cut(s) 230, 563
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 302
CsiI ACCWGGT 1 cut(s) 160
Csp6I GTAC 2 cut(s) 331, 643
CviAII CATG 5 cut(s) 293, 351, 547, 941, 1241
CviQI GTAC 2 cut(s) 331, 643
DdeI CTNAG 3 cut(s) 132, 584, 1319
DinI GGCGCC 1 cut(s) 229
DpnI GATC 5 cut(s) 234, 487, 516, 933, 1324
DpnII GATC 5 cut(s) 232, 485, 514, 931, 1322
DraIII CACNNNGTG 1 cut(s) 533
EaeI YGGCCR 4 cut(s) 87, 211, 711, 748
EagI CGGCCG 2 cut(s) 87, 211
Eam1104I CTCTTC 2 cut(s) 392, 1120
EarI CTCTTC 2 cut(s) 392, 1120
EciI GGCGGA 1 cut(s) 825
EclXI CGGCCG 2 cut(s) 87, 211
Eco130I CCWWGG 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 302
Eco52I CGGCCG 2 cut(s) 87, 211
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 5 cut(s) 296, 354, 550, 944, 1244
FaqI GGGAC 1 cut(s) 635
FatI CATG 5 cut(s) 292, 350, 546, 940, 1240
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 1 cut(s) 931
FokI GGATG 3 cut(s) 264, 644, 820
FspBI CTAG 7 cut(s) 44, 191, 506, 851, 984, 1232, 1362
GlaI GCGC 2 cut(s) 229, 562
GsuI CTGGAG 1 cut(s) 1262
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 4 cut(s) 89, 213, 713, 750
HapII CCGG 3 cut(s) 86, 204, 281
HhaI GCGC 2 cut(s) 230, 563
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 296, 354, 550, 944, 1244
Hin6I GCGC 2 cut(s) 228, 561
HinP1I GCGC 2 cut(s) 228, 561
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 1301
HinfI GANTC 3 cut(s) 18, 820, 1033
HpaII CCGG 3 cut(s) 86, 204, 281
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 3 cut(s) 472, 981, 1117
Hpy188I TCNGA 2 cut(s) 1167, 1264
Hpy8I GTNNAC 3 cut(s) 472, 981, 1117
Hpy99I CGWCG 4 cut(s) 98, 326, 613, 1124
HpyAV CCTTC 8 cut(s) 110, 451, 612, 647, 658, 849, 1124, 1346
HpyCH4III ACNGT 3 cut(s) 343, 953, 1298
HpyCH4IV ACGT 3 cut(s) 645, 1113, 1342
HpyCH4V TGCA 9 cut(s) 37, 57, 350, 407, 500, 524, 741, 1273, 1330
HpyF3I CTNAG 3 cut(s) 132, 584, 1319
HpySE526I ACGT 3 cut(s) 645, 1113, 1342
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 296, 354, 550, 944, 1244
HspAI GCGC 2 cut(s) 228, 561
KasI GGCGCC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 280
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 5 cut(s) 232, 485, 514, 931, 1322
LmnI GCTCC 2 cut(s) 427, 761
Lsp1109I GCAGC 8 cut(s) 91, 94, 397, 400, 419, 536, 1177, 1327
LweI GCATC 2 cut(s) 394, 572
MabI ACCWGGT 1 cut(s) 160
MaeI CTAG 7 cut(s) 44, 191, 506, 851, 984, 1232, 1362
MaeII ACGT 3 cut(s) 645, 1113, 1342
MaeIII GTNAC 3 cut(s) 728, 1085, 1245
MalI GATC 5 cut(s) 234, 487, 516, 933, 1324
MbiI CCGCTC 1 cut(s) 572
MboI GATC 5 cut(s) 232, 485, 514, 931, 1322
MboII GAAGA 7 cut(s) 137, 140, 175, 409, 1012, 1042, 1137
MflI RGATCY 2 cut(s) 485, 1322
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 4 cut(s) 167, 501, 1016, 1168
MluNI TGGCCA 1 cut(s) 750
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 1 cut(s) 814
Mox20I TGGCCA 1 cut(s) 750
MroI TCCGGA 1 cut(s) 280
MroNI GCCGGC 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 440
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 945, 1172, 1290
MslI CAYNNNNRTG 2 cut(s) 297, 1071
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 3 cut(s) 410, 1282, 1318
MspI CCGG 3 cut(s) 86, 204, 281
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 37
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 1038
NaeI GCCGGC 1 cut(s) 87
NarI GGCGCC 1 cut(s) 228
NdeII GATC 5 cut(s) 232, 485, 514, 931, 1322
NgoMIV GCCGGC 1 cut(s) 85
NheI GCTAGC 1 cut(s) 850
NlaIII CATG 5 cut(s) 296, 354, 550, 944, 1244
NlaIV GGNNCC 2 cut(s) 229, 487
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 728, 1085
NotI GCGGCCGC 1 cut(s) 211
NspI RCATGY 1 cut(s) 354
PaeI GCATGC 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 546
PctI GAATGC 1 cut(s) 37
PdiI GCCGGC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 2 cut(s) 18, 1033
PleI GAGTC 1 cut(s) 814
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 229, 487
PspPI GGNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 2 cut(s) 485, 1322
PvuII CAGCTG 3 cut(s) 410, 1282, 1318
RsaI GTAC 2 cut(s) 332, 644
RsaNI GTAC 2 cut(s) 331, 643
RseI CAYNNNNRTG 2 cut(s) 297, 1071
SaqAI TTAA 3 cut(s) 945, 1172, 1290
Sau3AI GATC 5 cut(s) 232, 485, 514, 931, 1322
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 814
ScrFI CCNGG 1 cut(s) 162
SexAI ACCWGGT 1 cut(s) 160
SfaNI GCATC 2 cut(s) 394, 572
SfcI CTRYAG 1 cut(s) 522
SfoI GGCGCC 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 2 cut(s) 297, 1071
SmlI CTYRAG 2 cut(s) 377, 862
SmoI CTYRAG 2 cut(s) 377, 862
SpeI ACTAGT 1 cut(s) 983
SphI GCATGC 1 cut(s) 354
Sse9I AATT 4 cut(s) 167, 501, 1016, 1168
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 1 cut(s) 871
SspMI CTAG 7 cut(s) 44, 191, 506, 851, 984, 1232, 1362
StyD4I CCNGG 1 cut(s) 160
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 3 cut(s) 343, 953, 1298
TaiI ACGT 3 cut(s) 648, 1116, 1345
TaqI TCGA 6 cut(s) 171, 261, 378, 627, 1022, 1211
TasI AATT 4 cut(s) 167, 501, 1016, 1168
TatI WGTACW 1 cut(s) 330
TauI GCSGC 4 cut(s) 92, 213, 216, 561
TfiI GAWTC 2 cut(s) 18, 1033
Tru1I TTAA 3 cut(s) 945, 1172, 1290
Tru9I TTAA 3 cut(s) 945, 1172, 1290
TscAI CASTG 2 cut(s) 714, 958
TseFI GTSAC 2 cut(s) 728, 1085
TseI GCWGC 8 cut(s) 82, 104, 407, 410, 413, 524, 1190, 1315
Tsp45I GTSAC 2 cut(s) 728, 1085
TspDTI ATGAA 3 cut(s) 17, 744, 908
TspGWI ACGGA 2 cut(s) 30, 1137
TspRI CASTG 2 cut(s) 714, 958
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 3 cut(s) 167, 1016, 1168
XbaI TCTAGA 2 cut(s) 505, 1231
XceI RCATGY 1 cut(s) 354
XhoI CTCGAG 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 7 cut(s) 44, 191, 506, 851, 984, 1232, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.