Rorug06G0226800

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0018380
Physical Location & Seq
Forward (+)
36444335 .. 36445147
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0226800.1

Sequence Viewer

Length: 813 bp
ATGCTTCAAGTAGTTGAGAGGGAACCATCTGGGAGGGCAGAAACATTTAGGATGCATGCTCTTCTGCGAGAACTCTGCATTTCAATTCCGGAAGCTGAGAGATTTTGTACCATACACAATGACCAAGAACCAGCTAAAGAGAGCAAGGCTCGCCACATGTCAATGCAAACGAATTCTCGAGCACTAAGAAATCTGAAAGACATTTTGAAGGTTCGCACATTTTTCATGTTTGCTGCTTTTTCATTGAATTATCTGCCATCTGGTTTCCAACTGTTGAGAATCCTAGACGTCCTTGAAGATGTTCCGATTGTACAGTTGCGAGATGACATAGTGAACTGCTTCAACGTGATGTATTTAAATTTGAGAAGCACAAAAGTTAAAGAGCTTCCCAAAGACATTGGGAAACTCAGCAACTTAGAAATGCTAAACATAAGCAATTCGAAAATTAGATTGCTTCCGGTTGGGATTTTGAAGTTACAGAAGCTTAACCGTCTCTTGATTTTGCATTTTAATGATCAAAAGTTGTGTTCTTTTGATTGTCTTGACGGCCGCACGCAAGCTCCGCCGGGCATTTGTAAGCTAAAGAGCTCACAAGTTCTGGATGCAGTCAAGGTACGTGGAAGGCGAGTTGGTCAAATAACTTCAGTCCATGATGCAACTCACAAGACTAGATTTCCGAAAGCTCACCGAGTTGTTACTGCTCAACTTTTCTCAATTGTCTTTGATCATCACAGAGAAGGGAGTGATGCAGCCATTAAAGACACTGGATATATGGGAATGCATGCAGTTGAAGCAAATTCCTCAGGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

270

Amino Acids

30.67

Weight (kDa)

9.53

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 70 - 206 2.3e-17 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24630
rosa_chinensis RchiOBHm_Chr6g0292461
rosa_laevigata RLG00000012022
rosa_multiflora Rmu_sc0001354.1_g000024
rosa_roxburghii Rroxscaffold_7G00175040
rosa_rugosa Rorug06G0226800
rosa_samantha Rh6AG340100 Rh6BG347400 Rh6DG340200
rosa_wichuraiana Rw6G029610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
AccIII TCCGGA 1 cut(s) 88
AciI CCGC 2 cut(s) 550, 563
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 172, 358, 796
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 3 cut(s) 109, 312, 615
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 8 cut(s) 8, 84, 208, 247, 296, 343, 472, 791
AluBI AGCT 8 cut(s) 95, 134, 385, 484, 560, 580, 588, 683
AluI AGCT 8 cut(s) 95, 134, 385, 484, 560, 580, 588, 683
Alw21I GWGCWC 2 cut(s) 184, 590
Alw26I GTCTC 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 177
Aor13HI TCCGGA 1 cut(s) 88
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 2 cut(s) 233, 749
ApoI RAATTY 3 cut(s) 172, 358, 796
Asp700I GAANNNNTTC 2 cut(s) 300, 338
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 1 cut(s) 677
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 177
AxyI CCTNAGG 1 cut(s) 802
BanII GRGCYC 1 cut(s) 590
Bbv12I GWGCWC 2 cut(s) 184, 590
BbvI GCAGC 2 cut(s) 220, 761
BccI CCATC 2 cut(s) 34, 265
BceAI ACGGC 1 cut(s) 562
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BclI TGATCA 2 cut(s) 514, 724
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 2 cut(s) 284, 669
BisI GCNGC 3 cut(s) 234, 550, 750
BlsI GCNGC 3 cut(s) 235, 551, 751
Bme1390I CCNGG 1 cut(s) 567
BmeT110I CYCGRG 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 567
BmsI GCATC 4 cut(s) 42, 592, 643, 736
BplI GAGNNNNNCTC 2 cut(s) 133, 165
Bpu14I TTCGAA 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 617
BsaHI GRCGYC 1 cut(s) 288
BsaWI WCCGGW 2 cut(s) 88, 457
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bse1I ACTGG 1 cut(s) 769
Bse21I CCTNAGG 1 cut(s) 802
BseAI TCCGGA 1 cut(s) 88
BseGI GGATG 2 cut(s) 57, 607
BseMII CTCAG 2 cut(s) 87, 421
BseNI ACTGG 1 cut(s) 769
BseX3I CGGCCG 1 cut(s) 547
BseXI GCAGC 2 cut(s) 220, 761
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 184, 590
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 3 cut(s) 89, 458, 566
BsmAI GTCTC 1 cut(s) 497
BsmBI CGTCTC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 184, 590
Bsp13I TCCGGA 1 cut(s) 88
Bsp1407I TGTACA 1 cut(s) 310
Bsp143I GATC 2 cut(s) 514, 724
BspACI CCGC 2 cut(s) 550, 563
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 2 cut(s) 88, 420
BspEI TCCGGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 66
BspT104I TTCGAA 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 310
BsrI ACTGG 1 cut(s) 769
BssMI GATC 2 cut(s) 514, 724
BssNI GRCGYC 1 cut(s) 288
Bst4CI ACNGT 3 cut(s) 273, 315, 491
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 310
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 5 cut(s) 57, 151, 554, 558, 783
BstDEI CTNAG 5 cut(s) 96, 185, 407, 415, 802
BstF5I GGATG 2 cut(s) 57, 607
BstKTI GATC 2 cut(s) 517, 727
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 2 cut(s) 514, 724
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 3 cut(s) 150, 562, 791
BstNSI RCATGY 3 cut(s) 59, 160, 785
BstSCI CCNGG 1 cut(s) 565
BstV1I GCAGC 2 cut(s) 220, 761
BstZI CGGCCG 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 802
BsuRI GGCC 1 cut(s) 549
BtsCI GGATG 2 cut(s) 57, 607
BtsIMutI CAGTG 1 cut(s) 762
Cac8I GCNNGC 5 cut(s) 57, 151, 554, 558, 783
Csp6I GTAC 3 cut(s) 108, 311, 614
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 5 cut(s) 56, 157, 226, 650, 782
CviQI GTAC 3 cut(s) 108, 311, 614
DdeI CTNAG 5 cut(s) 96, 185, 407, 415, 802
DpnI GATC 2 cut(s) 516, 726
DpnII GATC 2 cut(s) 514, 724
DraI TTTAAA 1 cut(s) 357
EaeI YGGCCR 1 cut(s) 547
EagI CGGCCG 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EciI GGCGGA 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 588
EclXI CGGCCG 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 590
Eco52I CGGCCG 1 cut(s) 547
Eco53kI GAGCTC 1 cut(s) 588
Eco57I CTGAAG 1 cut(s) 627
Eco81I CCTNAGG 1 cut(s) 802
Eco88I CYCGRG 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 588
EcoRI GAATTC 1 cut(s) 172
EcoT22I ATGCAT 2 cut(s) 57, 783
EcoT38I GRGCYC 1 cut(s) 590
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 5 cut(s) 59, 160, 229, 653, 785
FatI CATG 5 cut(s) 55, 156, 225, 649, 781
FbaI TGATCA 2 cut(s) 514, 724
Fnu4HI GCNGC 3 cut(s) 234, 550, 750
FokI GGATG 2 cut(s) 64, 614
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 3 cut(s) 234, 550, 750
FspBI CTAG 2 cut(s) 284, 669
GluI GCNGC 3 cut(s) 234, 550, 750
HaeIII GGCC 1 cut(s) 549
HapII CCGG 3 cut(s) 89, 458, 566
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 5 cut(s) 59, 160, 229, 653, 785
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 1 cut(s) 279
HpaII CCGG 3 cut(s) 89, 458, 566
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 3 cut(s) 195, 306, 678
Hpy188III TCNNGA 5 cut(s) 89, 177, 496, 542, 599
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 3 cut(s) 202, 615, 731
HpyCH4III ACNGT 3 cut(s) 273, 315, 491
HpyCH4IV ACGT 3 cut(s) 288, 345, 616
HpyCH4V TGCA 9 cut(s) 55, 78, 166, 505, 605, 656, 749, 781, 785
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 562, 791
HpyF3I CTNAG 5 cut(s) 96, 185, 407, 415, 802
HpySE526I ACGT 3 cut(s) 288, 345, 616
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 5 cut(s) 59, 160, 229, 653, 785
Kpn2I TCCGGA 1 cut(s) 88
Ksp22I TGATCA 2 cut(s) 514, 724
Kzo9I GATC 2 cut(s) 514, 724
LguI GCTCTTC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 565
LpnPI CCDG 9 cut(s) 15, 102, 144, 246, 471, 579, 584, 750, 789
Lsp1109I GCAGC 2 cut(s) 220, 761
LweI GCATC 4 cut(s) 42, 592, 643, 736
MaeI CTAG 2 cut(s) 284, 669
MaeII ACGT 3 cut(s) 288, 345, 616
MaeIII GTNAC 2 cut(s) 474, 694
MalI GATC 2 cut(s) 516, 726
MboI GATC 2 cut(s) 514, 724
MboII GAAGA 2 cut(s) 53, 308
MfeI CAATTG 1 cut(s) 714
MhlI GDGCHC 2 cut(s) 184, 590
MluCI AATT 8 cut(s) 84, 172, 247, 358, 436, 444, 714, 796
MmeI TCCRAC 1 cut(s) 292
MnlI CCTC 3 cut(s) 12, 27, 811
Mph1103I ATGCAT 2 cut(s) 57, 783
MroI TCCGGA 1 cut(s) 88
MroXI GAANNNNTTC 2 cut(s) 300, 338
MseI TTAA 5 cut(s) 356, 378, 486, 510, 756
MslI CAYNNNNRTG 2 cut(s) 161, 510
MspI CCGG 3 cut(s) 89, 458, 566
MspR9I CCNGG 1 cut(s) 567
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 783
MwoI GCNNNNNNNGC 3 cut(s) 150, 562, 791
NciI CCSGG 1 cut(s) 567
NdeII GATC 2 cut(s) 514, 724
NlaIII CATG 5 cut(s) 59, 160, 229, 653, 785
NlaIV GGNNCC 1 cut(s) 24
NsiI ATGCAT 2 cut(s) 57, 783
NspI RCATGY 3 cut(s) 59, 160, 785
NspV TTCGAA 1 cut(s) 440
PaeI GCATGC 2 cut(s) 59, 785
PaeR7I CTCGAG 1 cut(s) 177
PciI ACATGT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 2 cut(s) 300, 338
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 3 cut(s) 235, 551, 751
Ppu21I YACGTR 1 cut(s) 617
PscI ACATGT 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 590
PspN4I GGNNCC 1 cut(s) 24
RsaI GTAC 3 cut(s) 109, 312, 615
RsaNI GTAC 3 cut(s) 108, 311, 614
RseI CAYNNNNRTG 2 cut(s) 161, 510
SacI GAGCTC 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 66
SaqAI TTAA 5 cut(s) 356, 378, 486, 510, 756
SatI GCNGC 3 cut(s) 234, 550, 750
Sau3AI GATC 2 cut(s) 514, 724
ScrFI CCNGG 1 cut(s) 567
SduI GDGCHC 2 cut(s) 184, 590
SfaNI GCATC 4 cut(s) 42, 592, 643, 736
Sfr274I CTCGAG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 177
SmiI ATTTAAAT 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 161, 510
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
SphI GCATGC 2 cut(s) 59, 785
Sse9I AATT 8 cut(s) 84, 172, 247, 358, 436, 444, 714, 796
SsiI CCGC 2 cut(s) 550, 563
SspMI CTAG 2 cut(s) 284, 669
SstI GAGCTC 1 cut(s) 590
StyD4I CCNGG 1 cut(s) 565
SwaI ATTTAAAT 1 cut(s) 357
TaaI ACNGT 3 cut(s) 273, 315, 491
TaiI ACGT 3 cut(s) 291, 348, 619
TaqI TCGA 2 cut(s) 178, 440
TasI AATT 8 cut(s) 84, 172, 247, 358, 436, 444, 714, 796
TatI WGTACW 1 cut(s) 310
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 5 cut(s) 356, 378, 486, 510, 756
Tru9I TTAA 5 cut(s) 356, 378, 486, 510, 756
TscAI CASTG 1 cut(s) 769
TseI GCWGC 2 cut(s) 233, 749
TspDTI ATGAA 2 cut(s) 214, 231
TspRI CASTG 1 cut(s) 769
XapI RAATTY 3 cut(s) 172, 358, 796
XceI RCATGY 3 cut(s) 59, 160, 785
XhoI CTCGAG 1 cut(s) 177
XmnI GAANNNNTTC 2 cut(s) 300, 338
XspI CTAG 2 cut(s) 284, 669
ZraI GACGTC 1 cut(s) 289
Zsp2I ATGCAT 2 cut(s) 57, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.