RchiOBHm_Chr6g0304281

Salutaridinol 7-O-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0018386
Physical Location & Seq
Reverse (-)
63636503 .. 63637936
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27338

Sequence Viewer

Length: 1434 bp
ATGAAGGTATTGCAGGTAGAAGTTTTTTCTGAAGAGAAGATTAAACCATCTTTCCCAACACCCTCTCACCTCAGAACCTTCAAGCTTTCCCTTCTTGATCAGCTCATACCAGCTCCCTATGCTCCCATTATTCTCTTTTATGATCCCATTACTACCATTGCCACCACCAAAAGACTAGATGTGCTGAAGGCATCTTTATCTGAGGCCTTGACTGAGTTCTACCCCCTTGCCGGGAAGCTCAAAGATGATGAGCTATCTGTCGAGTGCAACGATGAAGGAGCTGCCTTCATCGAAGCCAGAGTTAAGTCCTTTTCCCTTCACGAGTTTCTCAGCCGTCCTGAACTTTTTGATTCGTTGCTGAACCAATTCCTCCCTGGAAATATTACTCATACGAGTAGTAGCAATTTTATTACAAACATCCAAGCAAATGTGTTCAAGTGCGGTGGCATTGCCATTGGCCTATGCATCTCACACAAGCTCCTTGATGGTGCTGCCTTGTGCACTTTCCTCAAGTCATGGACTGCTATTGGTCGTCGACTAGTATCAGGATCATCGGAGAAAGCAGCACCACCGCGTATATCCCCCAACTTATTTGCTGCGGCTTCTTTGTTCCCCACAAATGGCGATCTGTGGCTTAGAGATTCCTCTCTTCACATGTGGGGCTCCTTGTTCATAAAAGGCAAGGAGTCTAGTGTCACAAAAAGATTTGTGTTTGATGCCATGGCTATAGCCACCCTCAAGCTCAACGCTCGAATGGTTCGAAAGAAACCACCCACGCGCGTTGAGGTGGTTTCGGCTTTCTTGTGGCAGTGCTGCATGGCTGTCTCTAAAGAGAAACATGGCGTCCAAAGGCCTTCACTGCTGACACATATTGTGAACCTCCGCTCAAAACTACGGACTGCAATGGATGATTCCAATTCCAATCTTGAGCACTCTACCGGAAACCTCCTTTGGATGGCAGTCGCACGATCTTATATCGGAGATGTCGATGATGATGAGGAGGAGGAGGAGCAGCAGCAGAACTTGGTCTTGCCAGGCTTGGTGAGTGAGTTGAGTAATGCATTGTCGAAGATTGATGCTGATTTTGCCAAAGAAATCCGAGGGAAGCAAGGGAAGTCTCTAATGATTGATTCTCTGGAGGGAATCATGAACAGTGATGGGTTGGATTTAGATTATTATGGGTTTAGCAGTTGGTGCAAGTTTGGGTGGTACGAATCTGCTGATTTTGGGTGGGGGAAGCCTGCATGGGTGAGCAGCATTGGTTCCAATAGTGCTCCTCACTTCATGAATCTGATCATTTTGGTGGACACAAAGTCCGGTGACGGAATTGAAGCATGGGTCACCTTGGATCAGCGTCAGATGGCTCTACTGGAAGGCAACTCCAACCTTCAGAAATTGGTTTCAGTGGATCCTAGTCCTTTGATCTTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

477

Amino Acids

52.77

Weight (kDa)

5.8

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 460 2.2e-72 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g37440
malus_domestica MD15G1001600.v1.1
prunus_persica Prupe.1G354400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0304281
rosa_laevigata RLG00000010995
rosa_multiflora Rmu_sc0001652.1_g000008
rosa_roxburghii Rroxscaffold_7G00163880
rosa_rugosa Rorug06G0329600
rosa_samantha Rh6BG459400
rosa_wichuraiana Rw6G038440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 885
AccI GTMKAC 1 cut(s) 535
AccII CGCG 3 cut(s) 574, 778, 780
AciI CCGC 4 cut(s) 441, 572, 599, 883
AclWI GGATC 5 cut(s) 137, 556, 1356, 1403, 1416
AcuI CTGAAG 3 cut(s) 51, 206, 1373
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 1 cut(s) 1211
AfiI CCNNNNNNNGG 4 cut(s) 230, 231, 620, 955
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 3 cut(s) 82, 436, 1331
AhdI GACNNNNNGTC 1 cut(s) 1312
AhlI ACTAGT 1 cut(s) 538
AjnI CCWGG 2 cut(s) 373, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 934, 966
AluBI AGCT 8 cut(s) 85, 103, 113, 238, 253, 281, 478, 742
AluI AGCT 8 cut(s) 85, 103, 113, 238, 253, 281, 478, 742
Alw21I GWGCWC 3 cut(s) 503, 933, 1276
Alw26I GTCTC 2 cut(s) 829, 1122
Alw44I GTGCAC 1 cut(s) 499
AlwI GGATC 5 cut(s) 137, 556, 1356, 1403, 1416
AoxI GGCC 3 cut(s) 204, 457, 851
ApaLI GTGCAC 1 cut(s) 499
ApeKI GCWGC 8 cut(s) 281, 491, 563, 596, 813, 1012, 1015, 1254
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 780
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 5 cut(s) 59, 1054, 1261, 1331, 1333
AsuII TTCGAA 1 cut(s) 760
BaeGI GKGCMC 1 cut(s) 503
BamHI GGATCC 1 cut(s) 1408
BanII GRGCYC 1 cut(s) 665
BauI CACGAG 1 cut(s) 320
Bbv12I GWGCWC 3 cut(s) 503, 933, 1276
BbvI GCAGC 8 cut(s) 268, 478, 575, 583, 800, 1024, 1027, 1266
BccI CCATC 5 cut(s) 55, 479, 949, 1151, 1354
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 2 cut(s) 375, 1035
BclI TGATCA 2 cut(s) 97, 1293
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 829, 1122
BcuI ACTAGT 1 cut(s) 538
BfaI CTAG 4 cut(s) 176, 539, 690, 1413
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 9 cut(s) 282, 492, 564, 597, 600, 814, 1013, 1016, 1255
BlsI GCNGC 9 cut(s) 283, 493, 565, 598, 601, 815, 1014, 1017, 1256
Bme1390I CCNGG 3 cut(s) 232, 375, 1035
BmeRI GACNNNNNGTC 1 cut(s) 1312
BmiI GGNNCC 3 cut(s) 664, 1264, 1410
BmrFI CCNGG 3 cut(s) 232, 375, 1035
BmsI GCATC 4 cut(s) 200, 474, 706, 1066
BpmI CTGGAG 1 cut(s) 1157
Bpu14I TTCGAA 1 cut(s) 760
BpuEI CTTGAG 3 cut(s) 494, 722, 947
BpuMI CCSGG 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 843
BsaJI CCNNGG 4 cut(s) 373, 720, 1099, 1344
BsaWI WCCGGW 2 cut(s) 938, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 231, 620, 955
Bse1I ACTGG 1 cut(s) 1374
Bse3DI GCAATG 3 cut(s) 156, 447, 909
BseBI CCWGG 2 cut(s) 375, 1035
BseDI CCNNGG 4 cut(s) 373, 720, 1099, 1344
BseGI GGATG 3 cut(s) 417, 913, 960
BseLI CCNNNNNNNGG 4 cut(s) 230, 231, 620, 955
BseMI GCAATG 3 cut(s) 156, 447, 909
BseMII CTCAG 4 cut(s) 85, 192, 204, 343
BseNI ACTGG 1 cut(s) 1374
BseRI GAGGAG 5 cut(s) 1013, 1016, 1019, 1022, 1266
BseSI GKGCMC 1 cut(s) 503
BseXI GCAGC 8 cut(s) 268, 478, 575, 583, 800, 1024, 1027, 1266
Bsh1236I CGCG 3 cut(s) 574, 778, 780
BshFI GGCC 3 cut(s) 206, 459, 853
BsiHKAI GWGCWC 3 cut(s) 503, 933, 1276
BsiSI CCGG 3 cut(s) 231, 939, 1317
BslI CCNNNNNNNGG 4 cut(s) 230, 231, 620, 955
BsmAI GTCTC 2 cut(s) 829, 1122
BsnI GGCC 3 cut(s) 206, 459, 853
Bsp119I TTCGAA 1 cut(s) 760
Bsp1286I GDGCHC 4 cut(s) 503, 665, 933, 1276
Bsp143I GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 4 cut(s) 441, 572, 599, 883
BspANI GGCC 3 cut(s) 206, 459, 853
BspCNI CTCAG 4 cut(s) 84, 193, 205, 342
BspFNI CGCG 3 cut(s) 574, 778, 780
BspHI TCATGA 2 cut(s) 1146, 1284
BspLI GGNNCC 3 cut(s) 664, 1264, 1410
BspMI ACCTGC 1 cut(s) 4
BspPI GGATC 5 cut(s) 137, 556, 1356, 1403, 1416
BspT104I TTCGAA 1 cut(s) 760
BsrBI CCGCTC 1 cut(s) 885
BsrDI GCAATG 3 cut(s) 156, 447, 909
BsrI ACTGG 1 cut(s) 1374
BssECI CCNNGG 4 cut(s) 373, 720, 1099, 1344
BssMI GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
BssNI GRCGYC 1 cut(s) 843
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 2 cut(s) 720, 1344
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 2 cut(s) 375, 1035
Bst4CI ACNGT 1 cut(s) 1154
Bst6I CTCTTC 2 cut(s) 27, 654
BstACI GRCGYC 1 cut(s) 843
BstAPI GCANNNNNTGC 1 cut(s) 1194
BstBI TTCGAA 1 cut(s) 760
BstC8I GCNNGC 1 cut(s) 1242
BstDEI CTNAG 5 cut(s) 71, 201, 213, 329, 635
BstDSI CCRYGG 1 cut(s) 720
BstEII GGTNACC 1 cut(s) 1339
BstF5I GGATG 3 cut(s) 417, 913, 960
BstFNI CGCG 3 cut(s) 574, 778, 780
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 9 cut(s) 100, 145, 551, 628, 971, 1296, 1351, 1411, 1425
BstMAI GTCTC 2 cut(s) 829, 1122
BstMBI GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
BstMWI GCNNNNNNNGC 4 cut(s) 119, 859, 1085, 1194
BstNI CCWGG 2 cut(s) 375, 1035
BstNSI RCATGY 1 cut(s) 658
BstPI GGTNACC 1 cut(s) 1339
BstSCI CCNGG 3 cut(s) 230, 373, 1033
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 1 cut(s) 503
BstUI CGCG 3 cut(s) 574, 778, 780
BstV1I GCAGC 8 cut(s) 268, 478, 575, 583, 800, 1024, 1027, 1266
BstX2I RGATCY 1 cut(s) 1408
BstYI RGATCY 1 cut(s) 1408
BsuRI GGCC 3 cut(s) 206, 459, 853
BtgI CCRYGG 1 cut(s) 720
BtsCI GGATG 3 cut(s) 417, 913, 960
BtsI GCAGTG 2 cut(s) 815, 857
BtsIMutI CAGTG 4 cut(s) 815, 857, 1159, 1410
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 1 cut(s) 1242
CciI TCATGA 2 cut(s) 1146, 1284
CfoI GCGC 1 cut(s) 780
CseI GACGC 2 cut(s) 832, 1343
Csp6I GTAC 1 cut(s) 1210
CspCI CAANNNNNGTGG 4 cut(s) 424, 459, 763, 798
CviAII CATG 9 cut(s) 516, 655, 721, 817, 839, 1147, 1245, 1285, 1335
CviQI GTAC 1 cut(s) 1210
DdeI CTNAG 5 cut(s) 71, 201, 213, 329, 635
DpnI GATC 9 cut(s) 99, 144, 550, 627, 970, 1295, 1350, 1410, 1424
DpnII GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
DriI GACNNNNNGTC 1 cut(s) 1312
Eam1104I CTCTTC 2 cut(s) 27, 654
Eam1105I GACNNNNNGTC 1 cut(s) 1312
EarI CTCTTC 2 cut(s) 27, 654
Eco130I CCWWGG 2 cut(s) 720, 1344
Eco147I AGGCCT 2 cut(s) 206, 853
Eco24I GRGCYC 1 cut(s) 665
Eco57I CTGAAG 3 cut(s) 51, 206, 1373
Eco91I GGTNACC 1 cut(s) 1339
EcoO65I GGTNACC 1 cut(s) 1339
EcoRII CCWGG 2 cut(s) 373, 1033
EcoT14I CCWWGG 2 cut(s) 720, 1344
EcoT22I ATGCAT 2 cut(s) 467, 1063
EcoT38I GRGCYC 1 cut(s) 665
ErhI CCWWGG 2 cut(s) 720, 1344
FaeI CATG 9 cut(s) 519, 658, 724, 820, 842, 1150, 1248, 1288, 1338
FatI CATG 9 cut(s) 515, 654, 720, 816, 838, 1146, 1244, 1284, 1334
FbaI TGATCA 2 cut(s) 97, 1293
FblI GTMKAC 1 cut(s) 535
Fnu4HI GCNGC 9 cut(s) 282, 492, 564, 597, 600, 814, 1013, 1016, 1255
FokI GGATG 3 cut(s) 404, 920, 967
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 9 cut(s) 282, 492, 564, 597, 600, 814, 1013, 1016, 1255
FspBI CTAG 4 cut(s) 176, 539, 690, 1413
GlaI GCGC 1 cut(s) 779
GluI GCNGC 9 cut(s) 282, 492, 564, 597, 600, 814, 1013, 1016, 1255
GsuI CTGGAG 1 cut(s) 1157
HaeIII GGCC 3 cut(s) 206, 459, 853
HapII CCGG 3 cut(s) 231, 939, 1317
HgaI GACGC 2 cut(s) 832, 1343
HhaI GCGC 1 cut(s) 780
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 9 cut(s) 519, 658, 724, 820, 842, 1150, 1248, 1288, 1338
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 8 cut(s) 350, 641, 686, 911, 1130, 1143, 1214, 1288
HpaII CCGG 3 cut(s) 231, 939, 1317
HphI GGTGA 5 cut(s) 59, 1054, 1261, 1331, 1333
Hpy166II GTNNAC 4 cut(s) 501, 536, 877, 1306
Hpy188I TCNGA 9 cut(s) 31, 74, 202, 556, 980, 1100, 1293, 1359, 1392
Hpy188III TCNNGA 8 cut(s) 95, 320, 338, 546, 926, 1136, 1147, 1285
Hpy8I GTNNAC 4 cut(s) 501, 536, 877, 1306
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 9 cut(s) 88, 101, 181, 269, 295, 326, 864, 1367, 1397
HpyCH4III ACNGT 1 cut(s) 1154
HpyCH4V TGCA 9 cut(s) 13, 267, 465, 501, 816, 902, 1061, 1197, 1244
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 859, 1085, 1194
HpyF3I CTNAG 5 cut(s) 71, 201, 213, 329, 635
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 9 cut(s) 519, 658, 724, 820, 842, 1150, 1248, 1288, 1338
HspAI GCGC 1 cut(s) 778
Ksp22I TGATCA 2 cut(s) 97, 1293
Kzo9I GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
LmnI GCTCC 7 cut(s) 118, 127, 278, 483, 668, 1009, 1279
Lsp1109I GCAGC 8 cut(s) 268, 478, 575, 583, 800, 1024, 1027, 1266
LweI GCATC 4 cut(s) 200, 474, 706, 1066
MaeI CTAG 4 cut(s) 176, 539, 690, 1413
MaeIII GTNAC 3 cut(s) 694, 1319, 1339
MalI GATC 9 cut(s) 99, 144, 550, 627, 970, 1295, 1350, 1410, 1424
MbiI CCGCTC 1 cut(s) 885
MboI GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
MboII GAAGA 4 cut(s) 44, 49, 641, 1081
MflI RGATCY 1 cut(s) 1408
MhlI GDGCHC 4 cut(s) 503, 665, 933, 1276
MluCI AATT 6 cut(s) 365, 403, 916, 1326, 1394, 1429
MlyI GAGTC 1 cut(s) 695
MmeI TCCRAC 2 cut(s) 1143, 1407
Mph1103I ATGCAT 2 cut(s) 467, 1063
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 4 cut(s) 42, 303, 1427, 1432
MslI CAYNNNNRTG 1 cut(s) 1301
MspI CCGG 3 cut(s) 231, 939, 1317
MspR9I CCNGG 3 cut(s) 232, 375, 1035
MvaI CCWGG 2 cut(s) 375, 1035
MvnI CGCG 3 cut(s) 574, 778, 780
MwoI GCNNNNNNNGC 4 cut(s) 119, 859, 1085, 1194
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 720
NdeII GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
NlaIII CATG 9 cut(s) 519, 658, 724, 820, 842, 1150, 1248, 1288, 1338
NlaIV GGNNCC 3 cut(s) 664, 1264, 1410
NmuCI GTSAC 3 cut(s) 694, 1319, 1339
NsiI ATGCAT 2 cut(s) 467, 1063
NspI RCATGY 1 cut(s) 658
NspV TTCGAA 1 cut(s) 760
PagI TCATGA 2 cut(s) 1146, 1284
PceI AGGCCT 2 cut(s) 206, 853
PciI ACATGT 1 cut(s) 654
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 757, 984
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 7 cut(s) 350, 641, 911, 1130, 1143, 1214, 1288
PkrI GCNGC 9 cut(s) 283, 493, 565, 598, 601, 815, 1014, 1017, 1256
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PscI ACATGT 1 cut(s) 654
Psp6I CCWGG 2 cut(s) 373, 1033
PspEI GGTNACC 1 cut(s) 1339
PspGI CCWGG 2 cut(s) 373, 1033
PspN4I GGNNCC 3 cut(s) 664, 1264, 1410
PsuI RGATCY 1 cut(s) 1408
RsaI GTAC 1 cut(s) 1211
RsaNI GTAC 1 cut(s) 1210
RseI CAYNNNNRTG 1 cut(s) 1301
SalI GTCGAC 1 cut(s) 534
SaqAI TTAA 4 cut(s) 42, 303, 1427, 1432
SatI GCNGC 9 cut(s) 282, 492, 564, 597, 600, 814, 1013, 1016, 1255
Sau3AI GATC 9 cut(s) 97, 142, 548, 625, 968, 1293, 1348, 1408, 1422
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 3 cut(s) 232, 375, 1035
SduI GDGCHC 4 cut(s) 503, 665, 933, 1276
SfaNI GCATC 4 cut(s) 200, 474, 706, 1066
SfcI CTRYAG 1 cut(s) 726
SfuI TTCGAA 1 cut(s) 760
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 3 cut(s) 509, 737, 926
SmoI CTYRAG 3 cut(s) 509, 737, 926
SpeI ACTAGT 1 cut(s) 538
Sse9I AATT 6 cut(s) 365, 403, 916, 1326, 1394, 1429
SseBI AGGCCT 2 cut(s) 206, 853
SsiI CCGC 4 cut(s) 441, 572, 599, 883
SspI AATATT 1 cut(s) 382
SspMI CTAG 4 cut(s) 176, 539, 690, 1413
StuI AGGCCT 2 cut(s) 206, 853
StyD4I CCNGG 3 cut(s) 230, 373, 1033
StyI CCWWGG 2 cut(s) 720, 1344
TaaI ACNGT 1 cut(s) 1154
TaqI TCGA 7 cut(s) 261, 291, 535, 751, 760, 987, 1067
TasI AATT 6 cut(s) 365, 403, 916, 1326, 1394, 1429
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 7 cut(s) 350, 641, 911, 1130, 1143, 1214, 1288
Tru1I TTAA 4 cut(s) 42, 303, 1427, 1432
Tru9I TTAA 4 cut(s) 42, 303, 1427, 1432
TscAI CASTG 4 cut(s) 815, 864, 1159, 1410
TseFI GTSAC 3 cut(s) 694, 1319, 1339
TseI GCWGC 8 cut(s) 281, 491, 563, 596, 813, 1012, 1015, 1254
Tsp45I GTSAC 3 cut(s) 694, 1319, 1339
TspDTI ATGAA 7 cut(s) 17, 277, 288, 661, 1163, 1273, 1301
TspGWI ACGGA 2 cut(s) 910, 1338
TspRI CASTG 4 cut(s) 815, 864, 1159, 1410
VneI GTGCAC 1 cut(s) 499
XceI RCATGY 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 4 cut(s) 176, 539, 690, 1413
Zsp2I ATGCAT 2 cut(s) 467, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.